Clinical efficacy of laparoscopic surgery combined with drug therapy for endometriosis: A meta-analysis.

OBJECTIVE: To systematically investigate the clinical efficacy and prognosis of laparoscopic surgery combined with drug therapy for endometriosis. METHODS: Clinical randomized controlled trials regarding laparoscopic surgery combined with drug therapy for endometriosis and published between 2010 and 2022 were retrieved from PubMed, Web of Science and Embase, China National Knowledge Infrastructure, and Wanfang databases. Meta-analysis and sensitivity tests were performed using the Stata16.0 software. RESULTS: A total of 1332 articles were retrieved. Of them, 11 studies were included for final analysis, comprising of 521 cases in the treatment group (laparoscopic surgery combined with drug therapy) and 530 cases in the control group (laparoscopic surgery alone). Meta-analysis results showed that compared with the control group, the combined treatment had higher treatment effective rate [OR = 4.542, 95% CI (3.062, 6.736), P < 0.001], pregnancy rate [OR = 3.341, 95% CI (2.336, 4.780), P < 0.001], and lower incidence of adverse reactions [OR = 0.348, 95% CI (0.208, 0.583), P < 0.001] and recurrence rate [OR = 0.187, 95% CI (0.106, 0.328), P < 0.001]. In addition, after treatment, estradiol [SMD = -3.417, 95% CI (-4.631, -2.203), P < 0.001] and follicle-stimulating hormone [SMD = -1.976, 95% CI (-3.202, -0.751), P = 0.002] in the treatment group were significantly lower than the control group. CONCLUSION: Compared with laparoscopic surgery alone, laparoscopic surgery combined with drug therapy demonstrated better clinical efficacy and improved postoperative infertility and recurrence rate for treating endometriosis.

Gu-Ben-Zhi-Ke-Zhong-Yao Alleviated PM2.5-Induced Lung Injury via HMGB1/NF-κB Axis.

Background: Inhalation of particles with a diameter of less than 2.5 µm (PM2.5) among air pollutants may cause lung damage. Gu-Ben-Zhi-Ke-Zhong-Yao (GBZK) is a traditional Chinese medicine prescription that has a beneficial effect on the treatment of chronic obstructive pulmonary disease (COPD). However, the effect of GBZK on PM2.5-induced lung injury remains to be elucidated. Methods: We constructed a mice lung injury model through PM2.5 stimulation and simultaneously performed GBZK gavage treatment. After 4 weeks, the lung tissues of the mice were collected for pathological staining to analyze the degree of damage. The activities of myeloperoxidase (MPO), malondialdehyde (MDA), and oxidative stress-related factors (superoxide dismutase, SOD; glutathione peroxidase, GSH-Px) were detected by commercial kit in lung tissue. Furthermore, the number of neutrophils and related inflammatory factors (interleukin-1, IL-1ß; tumor necrosis factor α, TNF-α; interleukin-6, IL-6) in bronchoalveolar lavage fluid (BALF) and serum were collected and tested to evaluate the effect of GBZK on inflammation. Masson staining was used to detect the level of lung fibrosis in mice. The activation of HMGB1 (high-mobility group protein 1) and NFκBp65 (nucleus factor kappa B) in lung tissue was evaluated by immunohistochemistry and western blot. Results: The result revealed that PM2.5 induces lung damage, and GBZK gavage treatment could reduce the degree of injury in a concentration-dependent manner in mice. After GBZK treatment, the MPO activity, MDA content, and oxidative stress level in the lung tissues of mice decreased. And after GBZK treatment, the expression levels of inflammatory cytokines in BALF and blood were decreased. GBZK treatment also improved pulmonary fibrosis in mice. In addition, we also found that GBZK prevented the up-regulation of the HMGB1/NF-κB axis in the lungs of mice. Conclusion: These results indicated that GBZK might protect mice from PM2.5-induced lung injury by inhibiting the HMGB1/NFκB pathway, thus repressing inflammation and pulmonary fibrosis.

[Development and application of a mobile positive pressure clean chamber].

During the epidemic of coronavirus disease 2019, due to the need to collect a large number of nucleic acid samples, the staff are under great pressure. For this reason, the medical staff of China-Japan Friendship Hospital developed a mobile positive pressure clean chamber and applied for a national utility model patent (application number: 202021173605.8). The equipment is composed of a cabin body, an operation hole equipped with rubber gloves, an interactive channel with two electric doors, an environmental control unitandanair-conditioner. When in use, the medical staff are located inside the cabin, and their hands are protruded by two operating holes to calculate and sample for the tested personnel. Then the samples are placed on the table outside the cabin waiting for inspection. The clean chamber can be used in hospitals, communities and other places, while achieving the goal of efficient sampling, and the risk of infection in the process is reduced by effectively blocking the contact between medical staff and the source of infection.