RESUMO
Oroxylum indicum is one of the valuable Dai pharmaceuticals; the dry seeds and bark of O. indicum were used to treat acute cough, sore throat and so on. Of the seven compounds from O. indicum were determined and obtained using the bioassay-guided method. Among them, compound 7 was obtained from the plant for the first time. Eight bacterial strains and one yeast fungi were exposed to the compounds. Minimum inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) or minimum fungicidal concentrations were determined according to the standard broth microdilution method. Baicalein (2) exhibited relative strong antibacterial activities with MIC of 8 µg ml-1 and MBC of 16 µg ml-1 against three MRSA strains of Staphylococcus aureus of SCCmec III type, whereas flavonoids 3, 5 and 7 showed some degree of activities against methicillin-susceptible S. aureus (MSSA, ATCC 25923). The findings may offer new evidence that why O. indicum was used widely in Dai peoples' life.
Assuntos
Antibacterianos , Staphylococcus aureus Resistente à Meticilina , Extratos Vegetais/farmacologia , Antibacterianos/farmacologia , Bignoniaceae/química , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade MicrobianaRESUMO
AIM: To investigate the effects of 2-hydroxy-ethyl methacrylate (HEMA) on cytotoxicity and cyclooxygenase-2 (COX-2) protein expression in human osteoblasts. METHODOLOGY: Cytotoxicity was judged using an Alamar Blue reduction assay on human osteoblast cell line U2OS. Western blot was used to evaluate the expression of COX-2 protein by HEMA. To determine whether glutathione (GSH) levels were important in cytotoxicity and COX-2 expression of HEMA, cells were pre-treated with the GSH precursor, 2-oxothiazolidine-4-carboxylic acid (OTZ), to boost thiol levels, or buthionine sulfoximine (BSO) to deplete GSH. Paired Student's t-tests were applied for the statistical analysis of the results. RESULTS: HEMA demonstrated a cytotoxic effect to U2OS cells in a dose-dependent manner (P < 0.05). The 50% inhibition concentration of HEMA was approximately 3 mmol L(-1) . HEMA was found to induce COX-2 protein expression in U2OS cells (P < 0.05). The addition of OTZ acted as a protective effect on HEMA-induced cytotoxicity and COX-2 expression (P < 0.05). In contrast, the addition of BSO enhanced HEMA-induced cytotoxicity and COX-2 expression (P < 0.05). CONCLUSION: Taken together, the levels of HEMA that were tested inhibited cell growth on U2OS cells. HEMA has a significant potential for periapical toxicity. The activation of COX-2 protein expression may be one of the mechanisms of HEMA-induced periapical inflammation. These inhibitory effects were associated with intracellular GSH levels.
Assuntos
Ciclo-Oxigenase 2/metabolismo , Glutationa/metabolismo , Metacrilatos/metabolismo , Osteoblastos/metabolismo , HumanosRESUMO
AIM: To evaluate the expression of tumour necrosis factor-α and surface antigens by bisphenol A-glycidyl-methacrylate (BisGMA) on murine macrophage cell line RAW264.7. METHODOLOGY: Cytotoxicity was measured by tetrazolium bromide reduction assay. Tumour necrosis factor (TNF)-α was analysed by enzyme-linked immunosorbent assay. Cell surface antigens were investigated by flowcytometry. Statistical analyses were performed using anova followed by the Bonferroni's t-test for multigroup comparisons. RESULTS: BisGMA exhibited cytotoxicity to RAW264.7 in a dose-dependent manner (P < 0.05) during 2-h incubation period. BisGMA was found to increase TNF-α secretion in a dose-dependent manner (P < 0.05). In addition, CD11, CD14, CD45, CD54, CD40, CD80, and MHC II were significantly stimulated by BisGMA in a dose-dependent manner (P < 0.05). However, MHC I expression was not affected by BisGMA (P > 0.05). CONCLUSIONS: Taken together, the ability of macrophages to induce an appropriate immune response when exposed to BisGMA has the potential to upregulate TNF-α production and expression of surface antigens.
Assuntos
Bis-Fenol A-Glicidil Metacrilato/toxicidade , Macrófagos/efeitos dos fármacos , Fator de Necrose Tumoral alfa/biossíntese , Animais , Antígenos de Superfície/biossíntese , Antígenos de Superfície/genética , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Macrófagos/metabolismo , Complexo Principal de Histocompatibilidade/efeitos dos fármacos , Camundongos , Fator de Necrose Tumoral alfa/genética , Regulação para CimaRESUMO
AIM: To evaluate the potential toxicological implications of BisGMA on murine macrophage cell line RAW264.7. METHODOLOGY: Lactate dehydrogenase release, flow cytometry, Western blot and fluorometric assays were used to detect cell viability, mode of cell death and caspase activities, respectively. In addition, alkaline single-cell gel electrophoresis and cytokinesis-block micronucleus assays were applied to detect genotoxicity. Statistical analyses were performed using anova followed by the Bonferroni's t-test for multi-group comparisons test. RESULTS: BisGMA demonstrated a cytotoxic effect on RAW264.7 cells in a dose-dependent and a time-dependent manner (P < 0.05). BisGMA was found to induce two modes of cell death. The mode of cell death changed from apoptosis to necrosis as the concentrations of BisGMA elevated. Caspase-3, caspase-8 and caspase-9 activities were significantly induced by BisGMA in a dose-dependent manner (P < 0.05). Moreover, BisGMA exhibited genotoxicity via a dose-related increase in the numbers of micronucleus and DNA strand breaks (P < 0.05). CONCLUSIONS: Cytotoxicity and genotoxicity induced by BisGMA are mediated by DNA damage and caspase activation.
Assuntos
Bis-Fenol A-Glicidil Metacrilato/toxicidade , Caspases/efeitos dos fármacos , Citotoxinas/toxicidade , Dano ao DNA , Materiais Dentários/toxicidade , Macrófagos/efeitos dos fármacos , Mutagênicos/toxicidade , Animais , Apoptose/efeitos dos fármacos , Western Blotting , Caspase 3/efeitos dos fármacos , Caspase 8/efeitos dos fármacos , Caspase 9/efeitos dos fármacos , Técnicas de Cultura de Células , Morte Celular/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Relação Dose-Resposta a Droga , Ativação Enzimática/efeitos dos fármacos , Ativadores de Enzimas/toxicidade , Citometria de Fluxo , Fluorometria , L-Lactato Desidrogenase/efeitos dos fármacos , Camundongos , Testes para Micronúcleos , Necrose , Fatores de TempoRESUMO
AIM: To evaluate ex vivo the mechanisms of cytotoxicity of dentine bonding agents in human pulp cells in vitro. METHODOLOGY: Human pulp cells were obtained from impacted third molars with informed consent and then cultured using an explant technique. Set specimens from Clearfil SE Bond (CB), Prime & Bond 2.1 (PB), and Single Bond (SB) were eluted with culture medium. Cytotoxicity was judged using an assay of tetrazolium bromide reduction. To determine whether glutathione (GSH) levels were important in the cytotoxicity of dentine bonding agents, cells were pretreated with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or buthionine sulfoximine (BSO) to deplete GSH. Three replicates of each dentine bonding agents were performed in each test. All assays were repeated three times to ensure reproducibility. Statistical analysis was by one-way analysis of variance (anova). Tests of differences of the treatments were analysed by Duncan's test. RESULTS: Clearfil SE Bond, PB, and SB were cytotoxic to pulp cells in a concentration-dependent manner (P<0.05). The cytotoxicity was upregulated by dentine bonding agents in the following order: PB>SB>CB. Addition of OTZ extracellularly protected the pulp cells from dentine bonding agents-induced cytotoxicity (P<0.05). Addition of BSO enhanced pulp cell death on dentine bonding agents-induced cytotoxicity (P<0.05). CONCLUSIONS: Dentine bonding agents have significant potential for pulpal toxicity. GSH depletion could be the mechanism for dentine bonding agents-induced cytotoxicity.
Assuntos
Materiais Dentários/toxicidade , Polpa Dentária/efeitos dos fármacos , Adesivos Dentinários/toxicidade , Glutationa/metabolismo , Acetona/toxicidade , Análise de Variância , Bis-Fenol A-Glicidil Metacrilato/toxicidade , Butionina Sulfoximina/farmacologia , Células Cultivadas , Polpa Dentária/citologia , Polpa Dentária/metabolismo , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/farmacologia , Glutationa/efeitos dos fármacos , Humanos , Concentração Inibidora 50 , Ácidos Polimetacrílicos/toxicidade , Ácido Pirrolidonocarboxílico/farmacologia , Cimentos de Resina/toxicidade , Tiazolidinas/farmacologiaRESUMO
BACKGROUND AND OBJECTIVE: Cystatin C is a 13 kDa non-glycosylated, basic protein belonging to the cystatin family. It is consistently and dramatically upregulated in a variety of fibrotic diseases. However, little is known about the correlation between cystatin C and cyclosporine A-induced gingival overgrowth. The aim of this study was to compare cystatin C expression in normal, healthy gingival tissues and cyclosporine A-induced gingival overgrowth specimens and further explore the potential mechanism that may result in cystatin C expression. MATERIAL AND METHODS: Fifteen cyclosporine A-induced gingival overgrowth specimens and five normal gingival tissues were examined by immunohistochemistry. Three human gingival fibroblast (HGF) strains were established from crown-lengthening surgery. The reverse transcriptase-polymerase chain reaction was used to investigate the effects on HGFs exposed to cyclosporine A. In addition, predominant periodontal pathogens (Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis) and proinflammatory cytokines (interleukin-1alpha and tumor necrosis factor-alpha) were added to seek the possible regulatory mechanisms of cystatin C expression. RESULTS: The cystatin C staining in gingival tissue was stronger in the cyclosporine A-induced gingival overgrowth group than in the normal gingival group (p < 0.05). Intensive staining for cystatin C expression was observed mainly in the cytoplasm of fibroblasts, epithelial cells and inflammatory cells. Moreover, cystatin C expression was significantly higher in cyclosporine A-induced gingival overgrowth specimens with higher levels of inflammatory infiltrates (p < 0.05). A concentration of 200 ng/mL cyclosporine A was found to increase cystatin C expression in HGFs in a time-dependent manner (p < 0.05). The addition of periodontal pathogens and proinflammatory cytokines significantly increased the expression of cystatin C compared with cyclosporine A alone (p < 0.05). CONCLUSION: The increased ability of protein accumulation by cystatin C is one of several factors mediating cyclosporine A-induced gingival overgrowth. In addition, cyclosporine A may predispose to gingival overgrowth in inflammatory environments.
Assuntos
Ciclosporina/efeitos adversos , Cistatina C/metabolismo , Fibroblastos/metabolismo , Gengiva/metabolismo , Crescimento Excessivo da Gengiva/metabolismo , Imunossupressores/efeitos adversos , Regulação para Cima , Aggregatibacter actinomycetemcomitans/fisiologia , Células Cultivadas , Cistatina C/análise , Citoplasma/metabolismo , Citoplasma/ultraestrutura , Relação Dose-Resposta a Droga , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/patologia , Gengiva/efeitos dos fármacos , Gengiva/patologia , Crescimento Excessivo da Gengiva/induzido quimicamente , Crescimento Excessivo da Gengiva/patologia , Humanos , Mediadores da Inflamação/farmacologia , Interleucina-1beta/farmacologia , Porphyromonas gingivalis/fisiologia , Fatores de Tempo , Fator de Necrose Tumoral alfa/farmacologiaRESUMO
BACKGROUND AND OBJECTIVE: Cigarette smoking is a major risk factor in the development and further progression of periodontal diseases. However, little is known about how nicotine influences the expression of osteolytic mediators in cigarette smoking-associated periodontal diseases. The aim of this study was to investigate the expression of interleukin-1, interleukin-8, receptor activator of nuclear factor-kappaB ligand (RANKL), gelatinases and tissue-type plasminogen activator in U2OS cells (from the human osteosarcoma cell line) stimulated with nicotine. MATERIAL AND METHODS: Differences in the expression of interleukin-1, interleukin-8 and RANKL mRNAs, in response to exposure to various concentrations of nicotine (0, 0.125, 0.25, 0.5 and 1 mm) were evaluated in U2OS cells using the reverse transcription-polymerase chain reaction.In addition, the levels of interleukin-1, interleukin-8 and RANKL proteins were determined using enzyme-linked immunosorbent assays. The gelatinolytic and caseinolytic activities in nicotine treated-U2OS cells were demonstrated using gelatin and casein zymography, respectively. RESULTS: Nicotine was found to increase the expression of interleukin-1, interleukin-8 and RANKL mRNA and protein in U2OS cells (p < 0.05). The gelatin zymograms revealed that matrix metalloproteinase (MMP)-2 and MMP-9 were secreted by U2OS cells. The secretion of MMP-2 and MMP-9 occurred in a dose-dependent manner that was dependent on the concentration of nicotine (p < 0.05). Casein zymography exhibited a caseinolytic band with a molecular weight of 70 kDa, indicative of the presence of tissue-type plasminogen activator. Tissue-type plasminogen activator was also found to be up-regulated by nicotine in a dose-dependent manner (p < 0.05). CONCLUSION: Taken together, the results of the present study indicated that smoking modulation of bone destruction in periodontal disease may involve various osteolytic mediators, such as interleukin-1, interleukin-8, RANKL, MMP-2, MMP-9, and tissue-type plasminogen activator.
Assuntos
Nicotina/farmacologia , Agonistas Nicotínicos/farmacologia , Osteólise/fisiopatologia , Osteossarcoma/fisiopatologia , Regulação para Cima , Caseínas/efeitos dos fármacos , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Gelatinases/efeitos dos fármacos , Humanos , Interleucina-1/análise , Interleucina-8/efeitos dos fármacos , Metaloproteinase 2 da Matriz/efeitos dos fármacos , Metaloproteinase 9 da Matriz/efeitos dos fármacos , Peso Molecular , Nicotina/administração & dosagem , Agonistas Nicotínicos/administração & dosagem , Ligante RANK/efeitos dos fármacos , Fatores de Tempo , Ativador de Plasminogênio Tecidual/efeitos dos fármacosRESUMO
AIM: To compare oncostatin M (OSM) expression in clinically healthy and inflamed specimened human pulp tissue. METHODOLOGY: Thirty pulpal tissue specimens (15 clinically healthy and 15 inflamed) were obtained from extracted third molars with informed consent from patients. The levels of OSM were compared between clinically healthy pulp and inflamed pulp tissues using the semi-quantitative reverse-transcriptase polymerase chain reaction. In addition, immunohistochemistry was used to identify the in situ localization of OSM expression in pulp specimens. For testing of differences in the OSM between the clinically healthy and inflamed human dental pulps, the Wilcoxon-Mann-Whitney rank sum test was applied. Differences in OSM expression between tissue with low and high levels of inflammation were subsequently analysed by Fisher's exact test. RESULTS: Inflamed pulps exhibited significantly higher OSM mRNA gene expression than clinically healthy pulps (P < 0.05). Immunohistochemistry demonstrated that OSM expression was significantly higher in inflamed than clinically healthy pulps (P < 0.05). OSM staining was detected in odontoblasts, fibroblasts, inflammatory infiltrates and endothelial cells. CONCLUSIONS: Oncostatin M expression was elevated in inflamed pulp tissue. OSM is potentially involved in the disease process of pulpal inflammation.
Assuntos
Polpa Dentária/metabolismo , Inibidores do Crescimento/metabolismo , Oncostatina M/metabolismo , Pulpite/metabolismo , Regulação para Cima , Citoplasma/metabolismo , Citoplasma/patologia , Polpa Dentária/patologia , Células Endoteliais/metabolismo , Células Endoteliais/patologia , Endotélio Vascular/metabolismo , Endotélio Vascular/patologia , Fibroblastos/metabolismo , Fibroblastos/patologia , Inibidores do Crescimento/análise , Humanos , Imuno-Histoquímica , Odontoblastos/metabolismo , Odontoblastos/patologia , Oncostatina M/análise , Pulpite/patologia , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
AIM: To investigate the receptor activator of nuclear factor-kappa B (NF-kappaB) ligand (RANKL) in osteoblastic cells stimulated with inflammatory mediators. METHODOLOGY: The expression of RANKL in human osteoblastic cell line U2OS stimulated by pro-inflammatory cytokine interleukin (IL)-1alpha and black-pigmented bacteria Porphyromonas endodontalis was investigated by Western blot and enzyme-linked immunosorbent assay (ELISA). The significance of the results obtained from control and treated groups was statistically analysed by the paired Student's t-test. RESULTS: IL-1alpha was found to upregulate RANKL production in U2OS cells (P < 0.05). Investigations of the time dependence of RANKL expression in IL-1alpha-treated cells revealed a rapid accumulation of RANKL protein after 1 h of exposure; it remained elevated throughout the 24-h incubation period shown by Western blot and ELISA. In addition, P. endodontalis also increased RANKL expression in U2OS cells after 4-h incubation period demonstrated by Western blot and ELISA (P < 0.05). CONCLUSIONS: IL-1alpha and P. endodontalis may be involved in developing apical periodontitis through the stimulation of RANKL production.
Assuntos
Interleucina-1alfa/metabolismo , Osteoblastos/metabolismo , Periodontite Periapical/metabolismo , Porphyromonas endodontalis/química , Ligante RANK/biossíntese , Perda do Osso Alveolar/metabolismo , Western Blotting , Linhagem Celular Tumoral , Meios de Cultivo Condicionados/farmacologia , Ensaio de Imunoadsorção Enzimática , Expressão Gênica , Humanos , Mediadores da Inflamação/metabolismo , Interleucina-1alfa/farmacologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/microbiologia , Periodontite Periapical/imunologia , Periodontite Periapical/microbiologia , Ligante RANK/análise , Regulação para CimaRESUMO
AIM: To investigate the in situ location of oncostatin M (OSM) in epithelialized apical periodontitis lesions. METHODOLOGY: Thirty periapical lesions of pulpal origin were collected with informed consent from patients at the time of apical surgery. Tissue specimens were fixed with 10% buffered formalin overnight, dehydrated in an ascending series of graded alcohol, and embedded in paraffin. Five micron sections from formalin-fixed, paraffin-embedded specimens were examined by immunohistochemistry. In addition, another section from each specimen was stained with haematoxylin and eosin to assess the presence of inflammatory infiltrates. Differences in OSM expression between tissue with low and high levels of inflammation were subsequently analyzed by Fisher's exact test. RESULTS: Based on histological examination of haematoxylin and eosin stained sections, all specimens revealed the morphology of epithelialized apical periodontitis lesions. The results from immunohistochemistry demonstrated that OSM stain was detected in the inflammatory infiltrates, epithelium, connective tissue, and endothelium. The OSM signal was mainly expressed in endothelial cells (100%) followed by inflammatory cells (93.33%), epithelial cells (53.33%), and fibroblasts (16.67%). In addition, OSM expression was significantly higher in epithelialized apical periodontitis with higher levels of inflammatory infiltrates (P < 0.001). CONCLUSIONS: Oncostatin M was found to be expressed in epithelialized apical periodontitis lesions and would form part of the cytokine network involved in the disease process of apical periodontitis.
Assuntos
Oncostatina M/biossíntese , Periodontite Periapical/metabolismo , Perda do Osso Alveolar/metabolismo , Distribuição de Qui-Quadrado , Células Endoteliais/metabolismo , Células Epiteliais/metabolismo , Fibroblastos/metabolismo , Humanos , Técnicas Imunoenzimáticas , Oncostatina M/análise , Periodontite Periapical/patologia , Linfócitos T/metabolismoRESUMO
To date there has been very little data on the cytotoxic response of different cell lines to root canal sealers. The objective of this study was to determine the cytocompatibility of three different extracts of root canal sealers and to compare the cytotoxic response of these materials on two different primary human oral fibroblasts (derived from gingiva and buccal mucosa) and one permanent hamster cell line (V79 cells). Cytotoxicity was judged using an assay of tetrazolium bromide reduction. The results showed that root canal sealers (AH plus, Canals, and N2) were cytotoxic to primary human oral fibroblast cultures and V79 cells. It was found that N2 was the most toxic root canal sealer among those tested in all cultures. The toxicity decreased in an order of N2 > AH plus approximately = Canals. The sensitivity of toxicity depended on the materials tested and the cell culture system used.
Assuntos
Fibroblastos/efeitos dos fármacos , Gengiva/efeitos dos fármacos , Mucosa Bucal/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/toxicidade , Análise de Variância , Animais , Linhagem Celular/efeitos dos fármacos , Colorimetria , Corantes , Cricetinae , Cricetulus , Combinação de Medicamentos , Resinas Epóxi/toxicidade , Eugenol/toxicidade , Formaldeído/toxicidade , Gengiva/citologia , Humanos , Mucosa Bucal/citologia , Sais de Tetrazólio , Tiazóis , Óxido de Zinco/toxicidade , Cimento de Óxido de Zinco e Eugenol/toxicidadeRESUMO
Phenolic compounds are widely used in clinical dentistry as sedatives for the dental pulp, as disinfectants for caries, and as root canal medications. The pathobiological effects of various phenolic compounds on human dental pulp fibroblasts were investigated with Hoechst 33258 fluorescence assay and DNA precipitation assay. All phenolic compounds showed cytotoxicity in Hoechst 33258 fluorescence assay by inhibiting cellular DNA in a concentration-dependent manner. The 50% inhibition concentrations required to decrease the cellular DNA contents by guaiacol, phenol, eugenol, and thymol were 9.8, 4.5, 0.9, and 0.5 mM, respectively. However these phenolic compounds did not cause DNA single-strand breaks in cultured human pulp fibroblasts. These results indicate that phenolic compounds are cytotoxic agents but are without genotoxic effects on human pulp fibroblasts in vitro. However care should be taken to reduce the possibility of pulpal as well as periapical irritations from inadvertent extrusion of these substances in clinical usage.
Assuntos
Polpa Dentária/efeitos dos fármacos , Fenóis/toxicidade , Irrigantes do Canal Radicular/toxicidade , Bisbenzimidazol , Células Cultivadas , Dano ao DNA , Replicação do DNA/efeitos dos fármacos , Polpa Dentária/citologia , Relação Dose-Resposta a Droga , Eugenol/toxicidade , Fibroblastos/efeitos dos fármacos , Corantes Fluorescentes , Guaiacol/toxicidade , Humanos , Concentração Inibidora 50 , Timol/toxicidadeRESUMO
An intracanal medicine is often required because microorganisms in the dentinal tubes may be difficult to eliminate completely by instrumentation. Phenolic compounds are widely used in dental treatment as sedatives for the dental pulp or as disinfectants for caries and the root canal. In this study, propidium iodide fluorescence and DNA precipitation assay were used to investigated the cytotoxic and genotoxic effects of camphorated phenol and camphorated parachlorophenol on cultured human pulp fibroblasts in vitro. Both medicines reduced the content of double-stranded polynucleic acid of fibroblasts over a 24-h culture period in a concentration-dependent manner. Camphorated parachlorophenol was more cytotoxic than camphorated phenol. But, both medicines did not cause genotoxicity on pulp cells. The advantage of these experimental methods are simplicity and rapidity. Furthermore, this experimental system may be useful for preliminary cytotoxicity and genotoxicity screening of various dental medicines in vitro.
Assuntos
Anti-Infecciosos Locais/toxicidade , Cânfora/toxicidade , Clorofenóis/toxicidade , Polpa Dentária/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fenóis/toxicidade , Irrigantes do Canal Radicular/toxicidade , Células Cultivadas , Dano ao DNA , Polpa Dentária/citologia , Combinação de Medicamentos , Humanos , Testes de Mutagenicidade , Estatísticas não ParamétricasRESUMO
OBJECTIVE: The objective of this study was to examine the effects of sodium hypochlorite (NaOCl) and chlorhexidine (CHx) on cultured human periodontal ligament (PDL) cells in vitro. STUDY DESIGN: The effects of irrigation solutions on human PDL cells were evaluated by propidium iodide fluorescence cytotoxicity assay, protein synthesis assay, and mitochondrial activity. RESULTS: Both NaOCl and CHx were cytotoxic to human PDL cells in a concentration- and contact time-dependent manner. In addition, CHx inhibited protein synthesis in human PDL cells. Although NaOCl displayed cellular cytotoxicity, it showed no protein inhibition in the PDL cells. Furthermore, both NaOCl and CHx exhibited an inhibitory effect on mitochondrial activity on human PDL cells. CONCLUSIONS: This study suggests that these irrigation fluids may cause detrimental effects on vital tissues. Its clinical significance, however, needs to be evaluated further because concentration used, exposure time to the agent, and exposure surface area are important factors affecting the resulting effect.
Assuntos
Anti-Infecciosos Locais/toxicidade , Clorexidina/toxicidade , Ligamento Periodontal/efeitos dos fármacos , Irrigantes do Canal Radicular/toxicidade , Hipoclorito de Sódio/toxicidade , Células Cultivadas , Relação Dose-Resposta a Droga , Fluorescência , Humanos , Mitocôndrias/efeitos dos fármacos , Ligamento Periodontal/citologia , Inibidores da Síntese de Proteínas/toxicidadeRESUMO
The half-life of an insecticide on a treated object can be used as a crucial test for evaluating the residual effect. On cotton and nylon bednets, the half-life of deltamethrin was 65.6 and 55.4 days, whereas the half-life of permethrin on cotton and nylon bednets was 35.0 and 27.4 days. A deltamethrin-impregnated bednet (10 mg/m2) is effective for 6-7 months and costs less than DDT residual spraying (2g/m2) on the inner walls of rooms.
Assuntos
Roupas de Cama, Mesa e Banho , Inseticidas , Controle de Mosquitos , Piretrinas , Animais , Anopheles , Culex , Cobaias , Meia-Vida , Nitrilas , PermetrinaRESUMO
The sex-linkage inheritance of skin color was observed in some local breeds of chicken. The results showed that there was a pair of melanin genes PP in autosomes in Taihe Silky Fowl, an Id gene in sex chromosome in Xianju Fowl, Xiaoshan Fowl and Beijing You Fowl, and an id gene in sex chromosome in Langshan Fowl, Gushi Fowl and Taihe Silky Fowl, respectively. The offspring produced by crossing of Taihe Silky Fowl(Male) with the fowl(Female) carrying Id gene (such as Xianju Fowl, Xiaoshan Fowl and Beijing You Fowl) can autosexingly determinate by skin color, male chicken has yellow skin, female chicken has black skin.
Assuntos
Galinhas/genética , Cor , Ligação Genética , Cromossomos Sexuais , Pele , Animais , Feminino , MasculinoRESUMO
The surgical corrective result of complete blepharoptosis is often uncertain. With suspension by Frontalis aponeurosis flap under the brow, the author treated 73 cases satisfactorily, the results conforming to Fox's criteria of a normal eyelid. The incision is made in the skin and Orbicularis oculi at the double-fold of the eyelid, and a muscle flap 2 cm wide is separated from under the Orbicularis oculi at the brow in the conjunction of Orbicularis oculi and Frontalis. The flap is fixed to the tarsus, and a strip of redundant skin 2-3 mm wide excised. The incision is closed with intermittent sutures.
Assuntos
Blefaroptose/cirurgia , Retalhos Cirúrgicos/métodos , Adolescente , Adulto , Blefaroptose/congênito , Criança , Pré-Escolar , Pálpebras/cirurgia , HumanosRESUMO
BACKGROUND AND OBJECTIVE: Cyclosporin A is used as an immunosuppressive agent and its prominent side-effect is the induction of fibrous gingival overgrowth. The progression of fibrous gingival overgrowth results from the accumulation of extracellular matrix. Type I plasminogen activator inhibitor (PAI-1) acts as the inhibitor of extracellular matrix degradation and is involved in some fibrotic diseases. However, little is known about the correlation between PAI-1 and cyclosporin A-induced gingival overgrowth. The aim of this study was to investigate the effects of cyclosporin A on the expression of PAI-1 mRNA and protein in human gingival fibroblasts human gingival fibroblasts in vitro and to compare PAI-1 expression in normal healthy gingival tissues and cyclosporin A-induced gingival overgrowth specimens in vivo. MATERIAL AND METHODS: Quantitative reverse transcription-polymerase chain reaction and western blot assay were used to investigate the effects on human gingival fibroblasts exposed to cyclosporin A. In addition, 10 cyclosporin A-induced gingival overgrowth specimens and five normal gingival tissues were examined by immunohistochemistry. RESULTS: Investigations of the time dependence of PAI-1 mRNA expression in human gingival fibroblasts treated with 200 ng/ml of cyclosporin A revealed a rapid accumulation of the transcript: a significant signal was first detectable after 1 h of exposure and the signal remained elevated throughout the 24-h incubation period (p < 0.05). Cyclosporin A was also found to up-regulate PAI-1 protein in a time-dependent manner (p < 0.05). The PAI-1 staining in gingival tissue was stronger in the cyclosporin A-induced gingival overgrowth group than in the normal gingival group (p < 0.05). In the cyclosporin A-induced gingival overgrowth group, intensive staining for PAI-1 expression was observed mainly in the cytoplasm of fibroblasts, endothelial cells and inflammatory cells. CONCLUSION: These findings suggest that the up-regulation of PAI-1 may play an important part in the molecular pathogenesis of cyclosporin A-induced gingival overgrowth.
Assuntos
Ciclosporina/farmacologia , Fibroblastos/efeitos dos fármacos , Gengiva/efeitos dos fármacos , Imunossupressores/farmacologia , Inibidor 1 de Ativador de Plasminogênio/metabolismo , Regulação para Cima/efeitos dos fármacos , Células Cultivadas , Corantes , Citoplasma/efeitos dos fármacos , Citoplasma/metabolismo , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Fibroblastos/metabolismo , Gengiva/citologia , Crescimento Excessivo da Gengiva/metabolismo , Humanos , Inflamação , Fatores de TempoRESUMO
AIM: To evaluate the mechanisms of cytotoxicity of eugenol in human osteoblastic cells in vitro. METHODOLOGY: Cytotoxicity and cell proliferation assays were performed to elucidate the toxic effects of eugenol on the human osteoblastic cell line U2OS. Furthermore, the effects of antioxidants catalase (scavenger of H2O2), superoxide dismutase (SOD, an extracellular superoxide free radical scavenger) and N-acetyl-L-cysteine (NAC, a cell-permeable glutathione precursor) were added to discover the possible mechanisms of eugenol-induced cytotoxicity. Paired Student's t-test was applied for the statistical analysis of the results. RESULTS: Eugenol demonstrated a cytotoxic effect to U2OS cells in a dose-dependent manner (P < 0.05). The 50% inhibition concentration of eugenol was approximately 0.75 mmol L(-1). Eugenol also inhibited cell proliferation during a 4-day culture period (P < 0.05). Addition of NAC extracellularly protected the cells from eugenol-induced cytotoxicity (P < 0.05). Neither, SOD nor catalase provided any protective effects on eugenol-induced cytotoxicity (P > 0.05). CONCLUSIONS: The levels of eugenol tested inhibited growth and proliferation of U2OS cells. Eugenol has significant potential for periapical toxicity. These inhibitory effects were associated with glutathione levels.
Assuntos
Materiais Dentários/toxicidade , Eugenol/toxicidade , Osteoblastos/efeitos dos fármacos , Acetilcisteína/farmacologia , Antioxidantes/farmacologia , Catalase/farmacologia , Morte Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Sequestradores de Radicais Livres/farmacologia , Humanos , Teste de Materiais , Substâncias Protetoras/farmacologia , Superóxido Dismutase/farmacologia , Fatores de TempoRESUMO
AIM: To investigate the tissue type plasminogen activator (t-PA) activity in human pulp cells stimulated with Porphyromonas endodontalis (P. endodontalis) in the absence or presence of p38 inhibitor SB203580, mitogen-activated protein kinase kinase (MEK) inhibitor U0126 and phosphatidylinositaol 3-kinase (PI3K) inhibitor LY294002. METHODOLOGY: The supernatants of P. endodontalis were used to evaluate t-PA activity in human pulp cells using casein zymography and enzyme-linked immunosorbent assay (ELISA). Furthermore, to search for possible signal transduction pathways, SB203580, U0126 and LY294002 were added to test how they modulated the t-PA activity. RESULTS: The main casein secreted by human pulp cells migrated at 70 kDa and represented t-PA. Secretion of t-PA was found to be stimulated with P. endodontalis during 2-day cultured period (P < 0.05). From the results of casein zymography and ELISA, SB203580 and U0126 significantly reduced the P. endodontalis stimulated t-PA production respectively (P < 0.05). However, LY294002 lacked the ability to change the P. endodontalis stimulated t-PA production (P > 0.05). CONCLUSIONS: Porphyromonas endodontalis enhances t-PA production in human pulp cells, and the signal transduction pathways p38 and MEK are involved in the inhibition of t-PA.