[The role of atopic background in the genesis of allergic manifestations in intestinal parasitoses]. / Rolul terenului atopic în geneza manifestarilor alergice din parazitozele intestinale.

The previous literature and personal observations showed the predominant role of the atopic background in the appearance of the allergic manifestation in the parasitic infestation and especially in giardiasis. The lot investigated was made of 52 patients with allergic manifestations, also infested with Giardia intestinalis or with Ascaris lumbricoides. The hereditary collateral and personal allergic antecedents and the clearly deficient histaminopexy (1/20) were present in 1/2 of the patients. Summation of the various parameters of atopic background shows its presence in about 2/3 of the patients investigated. Association of the clinical involvement of trophallergens was present in 1/2 of the patients. The results show that the allergic manifestation in the intestinal parasitoses have a multifactorial determination. The atopic background is predominant in their development. These manifestations are often a result of alimentary allergy due to the absorption of alimentary antigens through the enteral mucous membrane affected by the parasite.

Some aspects of cellular and humoral immunity in bronchial asthma and allergic rhinitis.

Blood lymphocyte subpopulations were investigated in 50 patients with allergic diseases (38 with allergic rhinitis and 12 with allergic bronchial asthma). The total T lymphocyte (Tt) count (E rosette test), the active T lymphocyte (Ta) count (active E rosette test) and the B lymphocyte count (EAC rosette test) were determined and the results were correlated with: 1) lymphocyte sensitization in vitro to different allergens and to PPD; 2) circulating immune complexes; 3) serum histaminopexic capacity. In allergic rhinites a decrease of Tt and B lymphocytes and an increase of Ta lymphocytes was observed. In allergic bronchial asthma the Tt lymphocyte were found reduced. Lymphocyte reactivity to PPD in vitro was present in 100% of the cases and in vitro lymphocyte sensitivity to several allergens was observed in most of the cases. The histaminopexic capacity was absent or low in 75% of the patients with allergic rhinitis and in 66.6% of the patients with allergic bronchial asthma. The involvement of cellular mediated immunity in allergic diseases is discussed.

Test of leukocyte migration inhibition. II. Comparative study of the techniques in capillary tubes and in agarose.

The object of the paper was to estimate the advantages and disadvantages of two techniques of the leukocyte migration inhibition (LMI) test (in capillary tubes and in agarose) for the in vitro testing of delayed type hypersensitization. The capillary technique was carried out according to Soborg and Bendixen and the agarose technique according to Clausen. The antigen used in both techniques was PPD in a concentration of 10 micrograms/ml. The techniques were performed in parallel in two groups of individuals: 15 healthy controls and 20 patients with chronic hepatitis. It was observed that in the control group sensitization to tuberculin in vitro was detected by the capillary technique in 7 of the 15 subjects (47%) and by the agarose technique in 4 of the 15 subjects (27%). In chronic hepatitis sensitization to PPD was detected, by the capillary method, in 11 of the 20 patients (55%) and by the agarose method, in 9 of the 20 patients (45%). The results were consistent by the two methods in 18 of the 20 patients. In conclusion the LMI test in capillary tubes is preferable to that in agarose being simpler, more rapid, more sensitive and less expensive.