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Am J Hum Genet ; 94(5): 695-709, 2014 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-24746958

RESUMO

With recent rapid advances in genomic technologies, precise delineation of structural chromosome rearrangements at the nucleotide level is becoming increasingly feasible. In this era of "next-generation cytogenetics" (i.e., an integration of traditional cytogenetic techniques and next-generation sequencing), a consensus nomenclature is essential for accurate communication and data sharing. Currently, nomenclature for describing the sequencing data of these aberrations is lacking. Herein, we present a system called Next-Gen Cytogenetic Nomenclature, which is concordant with the International System for Human Cytogenetic Nomenclature (2013). This system starts with the alignment of rearrangement sequences by BLAT or BLAST (alignment tools) and arrives at a concise and detailed description of chromosomal changes. To facilitate usage and implementation of this nomenclature, we are developing a program designated BLA(S)T Output Sequence Tool of Nomenclature (BOSToN), a demonstrative version of which is accessible online. A standardized characterization of structural chromosomal rearrangements is essential both for research analyses and for application in the clinical setting.


Assuntos
Aberrações Cromossômicas/classificação , Análise Citogenética/classificação , Software , Terminologia como Assunto , Sequência de Bases , Análise Mutacional de DNA , Genoma Humano/genética , Humanos , Dados de Sequência Molecular , Alinhamento de Sequência
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