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1.
Adv Exp Med Biol ; 911: 23-32, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26987327

RESUMO

Phase I enzymes, including cytochrome P450, family 1, subfamily A, and polypeptide 2 (CYP1A2), are involved in the activation of carcinogens to reactive intermediates that are capable of binding covalently to DNA to form DNA adducts, potentially initiating the carcinogenic process. The aim of present study was to investigate the association of CYP1A2 gene polymorphisms and haplotypes with lung cancer risk. A case-control study was carried out on 105 lung cancer patients and 189 controls. To investigate three CYP1A2 polymorphisms: rs2472299, rs2470890, rs11072508 we used a high resolution melting analysis. We found significant allele associations (rs2470890 and rs2422299) with lung cancer risk. We searched for meaningful associations for all variants in the dominant, recessive, and additive genetic models. Genotype associations in the recessive model were of marginal significance for the same single nucleotide polymorphisms. A haplotype analysis included five variants with the frequency higher than 1 %. The haplotype "acc", present with the highest frequency, was associated with increased lung cancer risk (38.7 % vs. 31.5 %; OR 1.38; 95 %CI 0.95-2.01). On the contrary, rare haplotype "gtc" was significantly associated with decreased lung cancer risk in the Slovak population. In conclusion, the present study identified the risk alleles and haploid genotype associations of the CYP1A2 gene in lung cancer.


Assuntos
Citocromo P-450 CYP1A2/genética , Predisposição Genética para Doença , Haplótipos/genética , Neoplasias Pulmonares/genética , Polimorfismo de Nucleotídeo Único/genética , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Feminino , Seguimentos , Genótipo , Humanos , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Fatores de Risco
2.
Physiol Res ; 72(5): 543-555, 2023 11 28.
Artigo em Inglês | MEDLINE | ID: mdl-38015754

RESUMO

The development of methods for measuring blood pressure (BP) in newborns and small children has a rich history. Methods for BP measuring in adults had to be adapted to this age group. For measuring BP in direct invasive way, a suitable approach had to be found to access the arterial circulation through the umbilical and later radialis artery. Currently, results obtained from direct invasive BP measurement are considered the "gold standard". The development of non-invasive methods for BP measuring in newborns and children began with the use of von Basch's sphygmomanometer (1880). In 1899, Gustav Gärtner constructed the device, which was the basis for the flush method. After the discovery of the palpation and auscultation methods, these methods were also used for BP measurement in newborns and children, however, the BP values obtained in these ways were typically underestimated using excessively wide cuffs. From the auscultation method, methods utilizing ultrasound and infrasound to detect arterial wall movement and blood flow were later developed. The oscillometric method for BP measurement was introduced by E. J. Marey so early as in 1876. In 1912, P. Balard used the oscillometric technique to measure blood pressure in a large group of newborns. Through different types of oscillometers using various methods for detecting vascular oscillations (such as xylol method, impedance and volume plethysmography, etc.), the development has continued to assessment of vascular oscillations by modern sensor technology and software. For continuous non-invasive blood pressure measurement, the volume-clamp method, first described by Jan Penáz in 1968, was developed. After modification for use in newborns, application of the cuff to the wrist instead of the finger, it is primarily used in clinical physiological studies to evaluate beat-to-beat BP and heart rate pressure variability, such as in the determination of the baroreflex sensitivity.


Assuntos
Pressão Arterial , Determinação da Pressão Arterial , Adulto , Criança , Humanos , Recém-Nascido , Lactente , Pressão Sanguínea , Determinação da Pressão Arterial/métodos , Artérias , Dedos
3.
Physiol Res ; 72(S1): S1-S9, 2023 06 09.
Artigo em Inglês | MEDLINE | ID: mdl-37294113

RESUMO

During phototherapy of jaundiced newborns, vasodilation occurs in the skin circulation compensated by vasoconstriction in the renal and mesenteric circulation. Furthermore, there is a slight decrease in cardiac systolic volume, and blood pressure, as well as an increase in heart rate and discrete changes in the heart rate variability (HRV). The primary change during phototherapy is the skin vasodilation mediated by multiple mechanisms: 1) Passive vasodilation induced by direct skin heating effect of the body surface and subcutaneous blood vessels, modified by myogenic autoregulation. 2) Active vasodilation mediated via the mechanism provided by axon reflexes through nerve C-fibers and humoral mechanism via nitric oxide (NO) and endothelin 1 (ET-1). During and after phototherapy is a rise in the NO:ET-1 ratio. 3) Regulation of the skin circulation through the sympathetic nerves is unique, but their role in skin vasodilation during phototherapy was not studied. 4) Special mechanism is a photorelaxation independent of the skin heating. Melanopsin (opsin 4) - is thought to play a major role in systemic vascular photorelaxation. Signalling cascade of the photorelaxation is specific, independent of endothelium and NO. The increased skin blood flow during phototherapy is enabled by the restriction of blood flow in the renal and mesenteric circulation. An increase in heart rate indicates activation of the sympathetic system as is seen in the measures of the HRV. High-pressure, as well as low-pressure baroreflexes, may play important role in these adaptation responses. The integrated complex and specific mechanism responsible for the hemodynamic changes during phototherapy confirm adequate and functioning regulation of the neonatal cardiovascular system, including baroreflexes.


Assuntos
Coração , Hiperbilirrubinemia , Recém-Nascido , Humanos , Coração/fisiologia , Fototerapia , Pele/irrigação sanguínea , Vasodilatação/fisiologia , Óxido Nítrico
4.
Physiol Res ; 71(S2): S179-S186, 2022 12 31.
Artigo em Inglês | MEDLINE | ID: mdl-36647906

RESUMO

Phototherapy is the most effective non-invasive method of neonatal hyperbilirubinemia treatment. Application of this method can be associated with side effects including changes in the cardiovascular system. During phototherapy, the primary effects in the cardiovascular system include cutaneous vasodilation leading to skin hyperperfusion and subsequent redistribution of blood. The increased blood flow through the skin is associated with increased transepidermal water loss. Further effects include an increase in cerebral blood flow. Redistribution of blood to the cutaneous bed is compensated by hypoperfusion in the splanchnic area (mostly postprandial) and a significant reduction of the renal blood flow. Regarding closure/reopening of the ductus arteriosus, the results suggest that that phototherapy does not affect ductal patency. During phototherapy the cardiac output can be slightly reduced due to a decreased stroke volume, especially in preterm newborns. Systemic blood pressure is decreased and heart rate is elevated in both preterm and term newborns during phototherapy. The heart rate variability is slightly reduced. Symbolic dynamics analysis of the short-term HRV showed that during phototherapy the activity of the ANS regulating the heart rate is shifted towards the dominancy of the sympathetic activity. The responses in the cardiovascular system of premature/mature newborns without other pathology confirm a well physiologically functioning control of this system, even under specific conditions of phototherapy.


Assuntos
Permeabilidade do Canal Arterial , Coração , Recém-Nascido , Humanos , Coração/fisiologia , Permeabilidade do Canal Arterial/etiologia , Débito Cardíaco , Fototerapia/efeitos adversos , Fototerapia/métodos
5.
Physiol Res ; 70(S3): S349-S356, 2021 12 31.
Artigo em Inglês | MEDLINE | ID: mdl-35099253

RESUMO

To characterize the differences in baroreflex sensitivity (BRS), blood pressure (BP), heart rate (HR) and respiration rate (RR) in preterm infants with a similar postconceptional age reached by various combinations of gestational and postnatal ages. To detect potential sex differences in assessed cardiovascular parameters. The study included 49 children (24 boys and 25 girls), postconceptional age 34.6+/-1.9 weeks. Two subgroups of infants were selected with the similar postconceptional age (PcA) and current weight, but differing in gestational (GA) and postnatal (PnA) ages, as well as two matched subgroups of boys and girls. Blood pressure (BP) was recorded continuously using Portapres device (FMS). A stationary segment of 250 beat-to-beat BP values was analyzed for each child. Baroreflex sensitivity (BRS) was calculated by cross-correlation sequence method. Despite the same PcA age and current weight, children with longer GA had higher BRS, diastolic and mean BP than children with shorter GA and longer PnA age. Postconceptional age in preterm infants is a parameter of maturation better predicting baroreflex sensitivity and blood pressure values compared to postnatal age. Sex related differences in BRS, BP, HR and RR were not found in our group of preterm infants.


Assuntos
Barorreflexo , Pressão Sanguínea , Frequência Cardíaca , Recém-Nascido Prematuro , Nascimento Prematuro/fisiopatologia , Fatores Etários , Feminino , Idade Gestacional , Humanos , Lactente , Recém-Nascido , Masculino , Taxa Respiratória , Fatores Sexuais , Fatores de Tempo
6.
Physiol Res ; 69(Suppl 3): S443-S454, 2020 12 31.
Artigo em Inglês | MEDLINE | ID: mdl-33471544

RESUMO

Matrix metalloproteinases (MMPs) are a family of zinc-dependent metalloendopeptidases that degrades extracellular matrix (ECM) components. MMPs are associated with venous wall remodelling, proliferation, migration, phenotypic and functional transformation of vascular smooth muscle cells and ECM organization under the physiological and pathophysiological conditions. We investigated possible association of genetic promoter polymorphisms of MMP2 (rs243866), MMP8 (rs11225395), MMP9 (rs3918242) and TIMP2 (rs8179090) to varicose veins development in the Slovak population. Genomic DNA from 276 Slovak individuals (138 cases, 138 controls) was genotyped for selected SNPs (rs243866, rs11225395, rs3918242 and rs8179090) using the PCR-RFLP analysis. The data were analysed by chi-squared (chi2) test, logistic regression, and Mann-Whitney test. The risk of varicose veins development was evaluated in dominant, codominant and recessive genetic models. The statistical evaluation of selected polymorphisms in patients in all three genetic models has not shown a significant risk of varicose veins development. Our study has not shown the association between selected polymorphisms and increased risk of varicose veins development in Slovak population. More evidence with broaden sample size is needed.


Assuntos
Metaloproteinases da Matriz/genética , Inibidor Tecidual de Metaloproteinase-2/genética , Varizes/genética , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Masculino , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 8 da Matriz/genética , Metaloproteinase 9 da Matriz/genética , Pessoa de Meia-Idade , Projetos Piloto , Polimorfismo de Nucleotídeo Único , Regiões Promotoras Genéticas , Eslováquia/epidemiologia , Varizes/epidemiologia , Varizes/patologia , Adulto Jovem
7.
Eur J Med Res ; 14 Suppl 4: 280-3, 2009 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-20626117

RESUMO

OBJECTIVE: Although the emotion regulatory difficulties in patients with major depressive disorder (MDD) are predicted to associate with impaired cardiovascular autonomic regulation, the changes of cardiac vagal regulation MDD are incompletely understood. The aim of the study was to evaluate the respiratory sinus arrhythmia (as an index of cardiac vagal regulation) using the spectral analysis in high frequency band of the heart rate variability and the indices of deep breathing test in adolescent patients with major depressive disorder. MATERIAL AND METHOD: Twenty-eight adolescent girls were examined - 14 patients with major depressive disorder without pharmacological treatment (average age: 16.4 +/- 0.2 yr) and 14 healthy probands (control group) matched for age and gender. The respiratory sinus arrhythmia was evaluated using the spectral analysis in high frequency band of the heart rate variability (HF-HRV) and the parameters of deep breathing test (I-E, I/E). In addition, mean R-R interval was calculated. RESULTS: The adolescent patients with MDD has significantly reduced spectral activity in the HF-HRV and lower I/E, I-E parameters compared to marched health subjects (P<0.05). CONCLUSIONS: We conclude that the adolescents girls with MDD have reduced respiratory sinus arrhythmia indicating cardiac vagal dysregulation. Since impaired cardiac vagal regulation is associated with increased risk of cardiovascular morbidity, this finding underscores the importance of impaired autonomic neuro-cardiac integrity already in adolescents with major depressive disorder without pharmacological treatment.


Assuntos
Arritmia Sinusal/etiologia , Transtorno Depressivo Maior/fisiopatologia , Coração/inervação , Adolescente , Feminino , Frequência Cardíaca , Humanos , Nervo Vago/fisiopatologia
8.
Physiol Meas ; 30(1): 29-41, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19039163

RESUMO

The purpose of this paper is to investigate the effect of orthostatic challenge on recurrence plot based complexity measures of heart rate and blood pressure variability (HRV and BPV). HRV and BPV complexities were assessed in 28 healthy subjects over 15 min in the supine and standing positions. The complexity of HRV and BPV was assessed based on recurrence quantification analysis. HRV complexity was reduced along with the HRV magnitude after changing from the supine to the standing position. In contrast, the BPV magnitude increased and BPV complexity decreased upon standing. Recurrence quantification analysis (RQA) of HRV and BPV is sensitive to orthostatic challenge and might therefore be suited to assess changes in autonomic neural outflow to the cardiovascular system.


Assuntos
Pressão Sanguínea , Tontura/fisiopatologia , Frequência Cardíaca , Adolescente , Feminino , Humanos , Masculino , Decúbito Dorsal/fisiologia , Adulto Jovem
9.
Physiol Meas ; 29(7): 817-28, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18583725

RESUMO

Multiscale entropy (MSE) analysis provides information about complexity on various time scales. The aim of this study was to test whether MSE is able to detect autonomic dysregulation in young patients with diabetes mellitus (DM). We analyzed heart rate (HR) oscillations, systolic (SBP) and diastolic blood pressure (DBP) signals in 14 patients with DM type 1 and 14 age- and sex-matched healthy controls. SampEn values (scales 1-10) and linear measures were computed. HR: among the linear measures of heart rate variability significant differences between groups were only found for RMSSD (p = 0.043). MSE was significantly reduced on scales 2 and 3 in DM (p = 0.023 and 0.010, respectively). SBP and DBP: no significant differences were detected with linear measures. In contrast, MSE analysis revealed significantly lower SampEn values in DM on scale 3 (p = 0.039 for SBP; p = 0.015 for DBP). No significant correlations were found between MSE and linear measures. In conclusion, MSE analysis of HR, SBP and DBP oscillations is able to detect subtle abnormalities in cardiovascular control in young patients with DM and is independent of standard linear measures.


Assuntos
Pressão Sanguínea , Diabetes Mellitus Tipo 1/fisiopatologia , Entropia , Frequência Cardíaca , Adulto , Feminino , Humanos , Masculino
10.
J Physiol Pharmacol ; 58 Suppl 5(Pt 2): 781-90, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18204192

RESUMO

The present study was undertaken to evaluate the effects of hypercapnia on the cardiorespiratory control in normovolemic and hypovolemic rabbits during exogenous hyperthermia. Hypovolemia was induced by administration of furosemide, the body temperature (Tb) was gradually elevated to 42 degrees C by body surface heating. Subsequently, Tb was lowered back to the initial values by gradual cooling. Recordings were done at normothermia (TN=38 degrees C), during heating at 40 degrees C (TH1) and 42 degrees C (TH2), and in the course of Tb reverting toward the baseline values back at 40 degrees C (TC1), and finally after full return to the initial temperature value (TC2). The hypercapnic ventilatory response (HCVR) was estimated as the slope of minute volume (VE) on end-tidal CO2 (ETCO2) curves. We found that heating caused an increase in the VE slope in the normovolemic (NV), but not in hypovolemic (HV), rabbits. Between-group comparison revealed a significant increase in HCVR at TH2 and TC1 in NV vs. HV rabbits. Hypercapnia in hyperthermia (at TC2) was accompanied by a significant decrease in heart rate only in the hypovolemic group. Recovery of Tb was unaccompanied by appreciable changes in HCVR in either NV or HV groups. In the course of cooling, a decrease in heart rate during hypercapnic challenge was present in both group, in HV the drop was less prominent. We conclude that hypercapnia during heat stress in both normovolemic and hypovolemic rabbits is associated with altered cardiorespiratory responses. HCVR during exogenous hyperthermia is augmented in normovolemic, but not in hypovolemic, rabbits.


Assuntos
Febre/fisiopatologia , Hipercapnia/fisiopatologia , Hipovolemia/fisiopatologia , Mecânica Respiratória/fisiologia , Animais , Gasometria , Pressão Sanguínea/fisiologia , Volume Sanguíneo/efeitos dos fármacos , Temperatura Corporal/fisiologia , Diuréticos/farmacologia , Feminino , Furosemida/farmacologia , Frequência Cardíaca/fisiologia , Hematócrito , Concentração de Íons de Hidrogênio , Masculino , Coelhos
11.
Physiol Res ; 66(Suppl 2): S215-S226, 2017 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-28937236

RESUMO

The normal retinal development is interrupted by preterm birth and a retinopathy of prematurity (ROP) may develop as its consequence. ROP is characterized by aberrant vessel formation in the retina as a response to multiple risk factors influencing the process of retinal angiogenesis. Insulin-like growth factor I (IGF-1) and vascular endothelial growth factor (VEGF) play an important role in the process of normal retinal vascularization. Insufficient nutrition during the first 4 postnatal weeks results in low serum levels of IGF-1, which is essential for correct retinal vessels formation, ensuring survival of the newly formed endothelial cells. Low IGF-1 level results in stop of angiogenesis in the retina, leaving it avascular and prompting the onset of ROP. Keeping the newborns in a positive energetic balance by providing enough nutrients and energy has a beneficial impact on their growth, neurodevelopment and decreased incidence of ROP. The best way to achieve this is the early parenteral nutrition with the high content of nutrients combined with early enteral feeding by the own mother´s breast milk. Multiple studies confirmed the safety and efficacy of early aggressive nutrition but information about its long-term effects on the metabolism, growth and development is still needed.


Assuntos
Ingestão de Energia/fisiologia , Recém-Nascido Prematuro/metabolismo , Leite Humano/metabolismo , Estado Nutricional/fisiologia , Retina/crescimento & desenvolvimento , Retina/metabolismo , Animais , Nutrição Enteral/tendências , Humanos , Recém-Nascido , Fator de Crescimento Insulin-Like I/metabolismo , Retinopatia da Prematuridade/dietoterapia , Retinopatia da Prematuridade/metabolismo
12.
Physiol Res ; 66(Suppl 2): S257-S264, 2017 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-28937240

RESUMO

At present, there are insufficient information about baroreflex sensitivity (BRS) and factors that determine BRS in premature newborns. The objective of this study was to determine the relationship between BRS and the characteristics that reflecting the intrauterine development (gestational age and birth weight), as well as postnatal development (postconception age and the actual weight of the child at the time of measurement). We examined 57 premature infants, who were divided into groups according to gestational age and postconception age as well as birth weight, and weight at the time of measurement. Continuous and noninvasive registration of peripheral blood pressure (BP) was performed in every child within 2-5 min under standard conditions using a Portapres (FMS) device. The results showed a close correlation of baroreflex sensitivity, heart rate and respiratory rate with gestational age, postconception age, birth weight and actual weight at the time of measurement premature newborns. An increase in the characteristics (ages and weights) resulted in increased BRS and diastolic arterial pressure (DAP), and in decreased heart and respiratory rates. Baroreflex sensitivity in the first week was in the group of very premature newborns the lowest (4.11 ms/mmHg) and in the light premature newborns was almost double (8.12 ms/mmHg). BRS increases gradually in relation to postnatal (chronological) and to postconception age as well as to birth and actual weight. The multifactor analysis of BRS identified birth weight and postconception age as the best BRS predictors. The two independent variables together explained 40 % of interindividual BRS variability.


Assuntos
Barorreflexo/fisiologia , Peso ao Nascer/fisiologia , Pressão Sanguínea/fisiologia , Idade Gestacional , Frequência Cardíaca/fisiologia , Recém-Nascido Prematuro/fisiologia , Determinação da Pressão Arterial/métodos , Feminino , Humanos , Recém-Nascido , Masculino
13.
Physiol Res ; 66(Suppl 2): S203-S214, 2017 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-28937235

RESUMO

Heart rate (HR) and heart rate variability (HRV) in newborns is influenced by genetic determinants, gestational and postnatal age, and other variables. Premature infants have a reduced HRV. In neonatal HRV evaluated by spectral analysis, a dominant activity can be found in low frequency (LF) band (combined parasympathetic and sympathetic component). During the first postnatal days the activity in the high frequency (HF) band (parasympathetic component) rises, together with an increase in LF band and total HRV. Hypotrophy in newborn can cause less mature autonomic cardiac control with a higher contribution of sympathetic activity to HRV as demonstrated by sequence plot analysis. During quiet sleep (QS) in newborns HF oscillations increase - a phenomenon less expressed or missing in premature infants. In active sleep (AS), HRV is enhanced in contrast to reduced activity in HF band due to the rise of spectral activity in LF band. Comparison of the HR and HRV in newborns born by physiological vaginal delivery, without (VD) and with epidural anesthesia (EDA) and via sectio cesarea (SC) showed no significant differences in HR and in HRV time domain parameters. Analysis in the frequency domain revealed, that the lowest sympathetic activity in chronotropic cardiac chronotropic regulation is in the VD group. Different neonatal pathological states can be associated with a reduction of HRV and an improvement in the health conditions is followed by changes in HRV what can be use as a possible prognostic marker. Examination of heart rate variability in neonatology can provide information on the maturity of the cardiac chronotropic regulation in early postnatal life, on postnatal adaptation and in pathological conditions about the potential dysregulation of cardiac function in newborns, especially in preterm infants.


Assuntos
Adaptação Fisiológica/fisiologia , Frequência Cardíaca/fisiologia , Recém-Nascido Prematuro/fisiologia , Animais , Barorreflexo/fisiologia , Idade Gestacional , Humanos , Recém-Nascido , Sono/fisiologia
14.
APMIS ; 113(10): 720-3, 2005 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16309434

RESUMO

Cystic dysplasia of the rete testis (CDRT) is a very rare cause of a paediatric scrotal mass often associated with renal and other genitourinary tract anomalies. These complex malformations are probably due to a developmental defect of the mesometanephric system during embryogenesis. A case of asymptomatic scrotal swelling in a 4-year-old boy is presented. Ultrasonography, showed a cystic lesion of the left testis associated with absence of the left kidney. Orchiectomy was performed because of extensive gonad involvement. Pathologic examination revealed multiple, anastomosing, irregular cystic spaces of varying sizes and shapes predominantly located in the region of the rete testis. The cysts had spread irregularly, displacing the testicular parenchyma, which was subsequently compressed under the tunica albuginea. Preoperative diagnosis of CDRT is easy if age, precise localisation, characteristic ultrasonographic features and other genitourinary malformations are considered. Other paediatric cystic lesions should be included in the differential diagnosis. It is possible to cure CDRT by orchiectomy or by conservative treatment. Nowadays the later option is preferred, but diagnosis of CDRT must be precisely established and followed by careful monitoring.


Assuntos
Cistos/diagnóstico , Rede do Testículo/patologia , Doenças Testiculares/diagnóstico , Pré-Escolar , Cistos/patologia , Cistos/cirurgia , Humanos , Masculino , Orquiectomia , Doenças Testiculares/congênito , Doenças Testiculares/patologia , Doenças Testiculares/cirurgia
15.
Bratisl Lek Listy ; 106(12): 412-5, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16642667

RESUMO

BACKGROUND: Severe impairment of lung functions in meconium aspiration syndrome (MAS) often needs the application of combined therapeutic approach. In our recent study, surfactant lung lavage during asymmetric high-frequency jet ventilation (HFJV) removed more meconium than surfactant lavage during conventional ventilation, however, after the lavage excessive CO2 elimination was observed during HFJV. OBJECTIVES: We hypothesized that the combination of asymmetric HFJV during surfactant lung lavage and conventional ventilation in the post-lavage period may be of benefit in a rabbit model of MAS. METHODS: Suspension of human meconium in saline (25 mg/ml, 4 ml/kg) was instilled into the tracheal tube of conventionally ventilated (frequency, f, 30/min, inspiration time, Ti, 50%) anesthetized rabbits to cause a respiratory failure. Animals were then lavaged (10 ml/kg in 3 portions) with diluted surfactant (Curosurf, 100 mg of phospholipids/ml) or saline during asymmetric HFJV (f, 300/min, Ti, 70%). After the lavage, animals were ventilated conventionally (f, 30/min, Ti, 50%) for next 1 hour. RESULTS: Surfactant lung lavage during asymmetric HFJV removed more meconium pigments and solids than saline with HFJV (p < 0.05 or p < 0.01, respectively). Moreover, application of asymmetric HFJV facilitated the lavage fluid removal in both groups. In the post-lavage period, improved oxygenation, lung compliance, right-to-left pulmonary shunts, and reduced ventilatory requirements were found in the surfactant group (p < 0.05), while pCO2 was kept in the normal range. CONCLUSIONS: Surfactant lung lavage by asymmetric HFJV followed by conventional ventilation is advantageous combination in rabbits with MAS and may be tested in neonatal MAS (Tab. 2, Fig. 2, Ref. 12).


Assuntos
Produtos Biológicos/administração & dosagem , Lavagem Broncoalveolar , Ventilação em Jatos de Alta Frequência , Síndrome de Aspiração de Mecônio/terapia , Fosfolipídeos/administração & dosagem , Surfactantes Pulmonares/administração & dosagem , Respiração Artificial , Animais , Humanos , Recém-Nascido , Coelhos
16.
Physiol Res ; 51(3): 227-38, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12234114

RESUMO

The aim of this study was to ascertain the persistence of heart rate and blood pressure oscillations at the onset of voluntary apnea in humans and to assess the dependence of the fluctuations parameters on the chemoreceptor activity. In 24 young subjects (10 males, 14 females, mean age 20.4 years) heart rate (represented by its reciprocal value--RR-intervals), systolic blood pressure (SBP) and diastolic blood pressure (DBP) during controlled breathing (CB) of atmospheric air and oxygen followed by apnea were recorded continuously. The cosine functions were then fitted by nonlinear regression analysis to the heart rate, SBP and DBP oscillations during CB and at the onset of apnea. The parameters of oscillations were different during atmospheric air breathing compared to oxygen breathing. During oxygen breathing there was an increase of the RR-interval oscillations--relative bradycardia and enhanced magnitude of respiratory sinus arythmia. During apnea, the base level of the blood pressure oscillations was higher after breathing of atmospheric air compared to oxygen breathing. At least one cosine-like wave oscillation was present at the onset of apnea in the heart rate, SBP and DBP and the second wave was present in all assessed parameters in at least 70% of recordings. The oscillations in RR-intervals are, to some extent, independent of blood pressure oscillations. No significant gender differences were found either in the duration of breath holding or in the RR and SBP oscillations parameters.


Assuntos
Apneia/fisiopatologia , Arritmia Sinusal/fisiopatologia , Pressão Sanguínea/fisiologia , Frequência Cardíaca/fisiologia , Periodicidade , Adolescente , Adulto , Feminino , Humanos , Masculino , Oxigênio/administração & dosagem , Reflexo/fisiologia , Respiração , Volição/fisiologia
17.
Physiol Res ; 45(6): 439-47, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-9085374

RESUMO

The effects of different body temperature (BT) on the respiratory and cardiovascular parameters and respiratory reflexes were studied in 33 anaesthetized adult rabbits. Hyperthermia elicited panting with mean panting respiratory rate 199 +/- 14 x min-1 in all anaesthetized rabbits. Significant correlations between BT and frequency of breathing (positive), heart rate (positive) or tidal volume (negative) were found. Cooling was accompanied by considerable arterial hypotension. Duration of the Hering-Breuer reflex (HB) was reduced by the rise of BT. Intensity of the reflex (assessed as the ratio of the apnoeic pause to the mean duration of the previous 5 breaths) was unchanged up to the body temperature eliciting panting (41.15 +/- 0.08 degrees C) when it was greatly diminished. Defensive airway reflexes were also changed in hyperthermia. The duration as well as the intensity of nasal apnoea (Kratschmer's reflex) and laryngeal chemoreflex apnoea were decreased. The intensities of respiratory efforts in sneezing and laryngeal coughing were reduced. The expulsive reactions evoked by mechanical stimulation of the larynx were replaced by very shortlasting inhibition of breathing during panting. The results indicate that reflex control of breathing via the Hering-Breuer reflex and the ability to eliminate irritants from the airways are diminished during hyperthermia and panting in anaesthetized rabbits.


Assuntos
Febre/fisiopatologia , Hemodinâmica/fisiologia , Reflexo/fisiologia , Mecânica Respiratória/fisiologia , Animais , Apneia/fisiopatologia , Gasometria , Pressão Sanguínea/fisiologia , Temperatura Corporal/fisiologia , Chinchila , Tosse/fisiopatologia , Frequência Cardíaca/fisiologia , Laringe/fisiopatologia , Coelhos
18.
Physiol Res ; 46(4): 271-8, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9728492

RESUMO

In experiments on 51 healthy anaesthetized and paralyzed rabbits the changes in parameters of mechanics of breathing during high frequency jet ventilation (HFJV) were determined and the mechanisms responsible for these changes were investigated. In the first series of experiments with two groups of animals ventilated by HFJV with relative inspiratory time ti=0.5 and ti=0.7 airway resistance (Raw) after 5 h of HFJV in the ti=0.5 group increased from 1.14+/-0.05 to 2.31+/-0.09 kPal(-1) x s (P < or = 0.001), in the ti=0.7 group from 1.22+/-0.04 to 1.78+/-0.08 kPal(-1) x s (P < or = 0.01). Dynamic compliance (Cdyn) decreased in the ti=0.5 group from 0.041+/-0.004 to 0.017+/-0.001 l x kPa(-1) (P < or = 0.01) and in the ti=0.7 group from 0.034+/-0.003 to 0.022+/-0.002 l x kPa(-1) (P < or = 0.01). In the second series of experiments a group of animals was ventilated by HFJV after cervical vagotomy. The deterioration of Raw and Cdyn was significantly reduced in vagotomized rabbits in comparison to the controls without vagotomy. Finally, the study of phospholipid content in bronchoalveolar lavage fluid revealed no significant differences after 5 h of artificial ventilation or spontaneous breathing. These data indicate that HFJV results in changes in the parameters of mechanics of breathing in healthy lungs, which may be attenuated, but not fully eliminated, by bilateral cervical vagotomy. The decrease in Cdyn and increase in Raw are probably not due to changes in the pulmonary surfactant content.


Assuntos
Ventilação em Jatos de Alta Frequência , Mecânica Respiratória , Animais , Lavagem Broncoalveolar , Fosfolipídeos/análise , Coelhos , Tensão Superficial
19.
Braz J Med Biol Res ; 35(8): 991-1000, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12185393

RESUMO

Physical exercise is associated with parasympathetic withdrawal and increased sympathetic activity resulting in heart rate increase. The rate of post-exercise cardiodeceleration is used as an index of cardiac vagal reactivation. Analysis of heart rate variability (HRV) and complexity can provide useful information about autonomic control of the cardiovascular system. The aim of the present study was to ascertain the association between heart rate decrease after exercise and HRV parameters. Heart rate was monitored in 17 healthy male subjects (mean age: 20 years) during the pre-exercise phase (25 min supine, 5 min standing), during exercise (8 min of the step test with an ascending frequency corresponding to 70% of individual maximal power output) and during the recovery phase (30 min supine). HRV analysis in the time and frequency domains and evaluation of a newly developed complexity measure - sample entropy - were performed on selected segments of heart rate time series. During recovery, heart rate decreased gradually but did not attain pre-exercise values within 30 min after exercise. On the other hand, HRV gradually increased, but did not regain rest values during the study period. Heart rate complexity was slightly reduced after exercise and attained rest values after 30-min recovery. The rate of cardiodeceleration did not correlate with pre-exercise HRV parameters, but positively correlated with HRV measures and sample entropy obtained from the early phases of recovery. In conclusion, the cardiodeceleration rate is independent of HRV measures during the rest period but it is related to early post-exercise recovery HRV measures, confirming a parasympathetic contribution to this phase.


Assuntos
Exercício Físico/fisiologia , Frequência Cardíaca/fisiologia , Sistema Nervoso Parassimpático/fisiologia , Adulto , Análise de Variância , Entropia , Tolerância ao Exercício/fisiologia , Humanos , Masculino , Descanso/fisiologia
20.
J Pediatr Endocrinol Metab ; 12(3): 423-31, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10821222

RESUMO

The aim of this study was to obtain information about parameters of heart rate variability (HRV) in three frequency bands (high frequency - HF, low frequency - LF and very low frequency - VLF), the sensitivity of cardiovascular tests, and subjective feelings depending on autonomic nervous system balance in a group of young patients with diabetes mellitus type 1 (IDDM). Sixty-four subjects were examined: 32 patients with diabetes with a mean age of 16.1 +/- 0.7 years and a mean duration of IDDM of 6.3 +/- 0.8 years, and 32 healthy controls matched for age, sex and BMI. Shorter R-R intervals and abnormal values reflecting HRV were found in the diabetic group. In particular, parameters of total power, and HF and LF bands were reduced. The ratio VLF/HF power revealed predominance of sympathetic tone in the diabetic subjects. Although relative power VLF was increased in the supine position, the reactive rise of the VLF band activity in orthostasis was lower in the IDDM group. Using cardiovascular tests (deep breathing, Valsalva, orthostasis), significant differences in reactions were found only in the deep breathing test. Evaluation of sympathetic:parasympathetic:indifferent subjective feelings by questionnaire did not reveal any differences between the diabetic and healthy groups.


Assuntos
Doenças do Sistema Nervoso Autônomo/fisiopatologia , Diabetes Mellitus Tipo 1/fisiopatologia , Frequência Cardíaca/fisiologia , Coração/fisiopatologia , Adolescente , Adulto , Doenças do Sistema Nervoso Autônomo/diagnóstico , Pré-Escolar , Feminino , Humanos , Hipotensão Ortostática/fisiopatologia , Lactente , Masculino , Sistema Nervoso Parassimpático/fisiopatologia , Postura/fisiologia , Mecânica Respiratória/fisiologia , Decúbito Dorsal/fisiologia , Sistema Nervoso Simpático/fisiopatologia , Manobra de Valsalva/fisiologia
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