Simple Protein Analysis by Droplet Paper Spray Ionization Mass Spectrometry with Polyolefin Silica-Based Paper.

Paper spray ionization mass spectrometry (PSI MS) has emerged as a notable method for the rapid analysis of biological samples. However, the typical cellulose-based paper tip is incompatible with protein detection due to the strong interaction between cellulose hydroxyl groups and proteins. In this study, we utilized a commercially available polyolefin-based synthetic paper, Teslin®, as an alternative PSI substrate for simple protein analysis. We have named this method "droplet PSI" MS, as the aqueous protein solution droplet retains its shape on the Teslin® paper tip. For droplet PSI, no further chemical pretreatment was necessary for the Teslin® substrate; the only required preparation was shaping the Teslin® paper into a triangular tip. In droplet PSI MS, protein ion signals were instantly detected from a protein solution droplet upon applying a spray solvent in situ along with high voltage (HV). When compared with conventional PSI MS, our method demonstrated superior sensitivity. The droplet PSI MS utilizing Teslin® also showcased flexibility in real-time observation of protein alterations induced by an acid additive. Additionally, the effects of spray solvent composition and the application method were discussed.

A Rectangular Notch-Band UWB Antenna with Controllable Notched Bandwidth and Centre Frequency.

This paper presents the design and realization of a compact ultra-wideband (UWB) antenna with a rectangular notch wireless area network (WLAN) band that has controllable notched bandwidth and center frequency. The UWB characteristics of the antenna are achieved by truncating the lower ends of the rectangular microstrip patch, and the notch characteristics are obtained by using electromagnetic bandgap (EBG) structures. EBGs consist of two rectangular metallic conductors loaded on the back of the radiator, which is connected to the patch by shorting pins. A rectangular notch at the WLAN band with high selectivity is realized by tuning the individual resonant frequencies of the EBGs and merging them. Furthermore, the results show that the bandwidth and frequency of the rectangular notch band could be controlled according to the on-demand rejection band applications. In the demonstration, the rectangular notch band was shifted to X-band satellite communication by tuning the EBG parameters. The simulated and measured results show that the proposed antenna has an operational bandwidth from 3.1-12.5 GHz for |S11| < -10 with a rectangular notch band from 5-6 GHz, thus rejecting WLAN band signals. The antenna also has additional advantages: the overall size of the compact antenna is 16 × 25 × 1.52 mm3 and it has stable gain and radiation patterns.