RESUMO
A headspace-solid phase microextraction-gas chromatography-mass spectrometry methodï¼HS-SPME-GC-MSï¼ was adopted for the quantitative study of 4-allylanisole, methyl eugenol, 2,3,5-trimethoxytoluene, 3,4,5-trimethoxytoluene, sarisan, 3,5-dimethoxytoluene and safrole in mice brain, liver tissues and blood after intragastric administration of Asari Radix et Rhizoma. A VF-WAXms (30 m×0.25 mm, 0.25 µm film thickness) capillary column and SPME fiber coated with 65 µm polydimethylsiloxane/divinylbenzene (PDMS/DVB) were used. The calibration curves of seven volatile constituents were established to validate the method's stability (RSD<15%), repeatability (RSD<9.5%), accuracy (RSD<22%), relative recovery (87.0%-108%) and extraction recovery (74.9%-102%). The validated HS-SPME-GC-MS assay was applied to determine the concentrations of seven constituents in liver, brain and blood. The detected contents were 0.22,0.14 µgâ¢g⻹,0.25 mgâ¢L⻹ (4-allylanisole), 1.1, 0.39 µgâ¢g⻹, 0.69 mgâ¢L⻹ (methyl eugenol), 0.45, 0.13 µgâ¢g⻹, 0.54 mgâ¢L⻹ (2,3,5-trimethoxytoluene), 0.51, 0.15 µgâ¢g⻹, 0.45 mgâ¢L⻹ (3,4,5-trimethoxytoluene), 0.48, 0.039 µgâ¢g⻹, 0.69 mgâ¢L ⻹ (sarisan), 2.2, 1.2 µgâ¢g⻹, 1.5 mgâ¢L⻹ (3,5-dimethoxytoluene) and 1.3, 0.67 µgâ¢g⻹, 1.1 mgâ¢L⻹ (safrole) respectively. This HS-SPME-GC-MS method is rapid and convenient, with a small sample size, and applicable for the analysis and determination of volatile constituents in traditional Chinese medicines, which provides scientific data for further studies on effective substances and toxic substances in Asari Radix et Rhizoma.