In vivo inhibitory activity of andrographolide derivative ADN-9 against liver cancer and its mechanisms involved in inhibition of tumor angiogenesis.

It is well known that liver cancer is a highly aggressive malignancy with poor prognosis. Andrographolide (AD), a major bioactive component of Andrographis paniculata (Burm. F.), is a potential anti-cancer pharmacophore and the synthesis of AD derivatives with better cytotoxicity to cancer cells has attracted considerable attentions. In the present study, we evaluated the in vivo inhibitory effects of ADN-9, a 15-benzylidene substituted derivative of AD, on the growth and metastasis of murine hepatoma H22 using an orthotopic xenograft model and a subcutaneous xenograft model, and we further studied the anti-angiogenic action and the related mechanisms of ADN-9 in vivo and in vitro. Importantly, ADN-9 remarkably suppressed the growth and metastasis of both orthotopic and subcutaneous xenograft tumors, and the serum AFP level in orthotopic hepatoma-bearing mice treated with 100mg/kg ADN-9 (ig.) was decreased to the normal level. We also found that ADN-9 showed stronger abilities than AD in shrinking tumors, suppressing the invasion and metastasis of H22 cells, decreasing the MVD and promoting tumor cell apoptosis in subcutaneous xenograft of mice. Additionally, ADN-9 exhibited stronger inhibitory activity than AD against the migration and VEGF-induced capillary-like tube formation in HUVECs, which was further proved to be associated with attenuating VEGF/VEGFR2/AKT signaling pathway. The present research provides the first evidence that a 15-substituted AD derivative is more promising than the parent compound in therapeutic treatment of liver cancer.

A facile fabrication of conjugated fluorescent nanoparticles and micro-scale patterned encryption via high resolution inkjet printing.

Conjugated fluorescent materials are getting more and more attention in the biomedical arena due to their high fluorescence intensity, non-bleaching and good biocompatibility. However, conjugated fluorescent materials are still not widely used in the field of anti-counterfeiting and pattern encryption due to their extremely low solubility and enormous difficulties in processing. Here, we use a facile approach to fabricate conjugated polymer fluorescent nanoparticles through a classic micro-emulsion method to address these issues. The particle size, loading materials and fluorescence intensity can be tuned as demanded. Later, these particles are transformed into invisible inks for inkjet printers to achieve micro-scale pattern encryption. These patterns show an ultra-high accuracy of around 30 micrometres. They can be used as QR codes for information encryption with 3 times more information encryption and great anti-counterfeiting ability. Finally, we establish an identification recognition system to check their validity. The scenario is the patient identification system of a hospital. The results show that these tags can be read in less than 3 seconds and they can last for 12 months at least. This facile approach holds great potential and bright prospects in the field of privacy protection, information encryption and anti-counterfeiting.

An invisible private 2D barcode design and implementation with tunable fluorescent nanoparticles.

The popularity of 2D barcodes is playing a key role in simplifying people's daily life activities, such as identification, quick payment, checking in and checking out, etc. However, relevant issues have emerged as their popularity has soared. The most urgent and representative problem is decryption, which may lead to serious information leakage and substantial damage to organizations, such as governments and international enterprises. This issue is mainly due to the visibility of 2D barcodes. In order to prevent potential privacy violation and sensitive information leakage through easy access of those visible 2D barcodes, we have designed and fabricated invisible 2D barcodes that will only be visible under UV illumination. This approach provides a promising solution to address the previous problem by transferring 2D barcodes into an invisible state. We have employed a typical micro-emulsion method to fabricate polystyrene (PS) fluorescent nanoparticles due to its simplicity. The invisible patterns can and will only be accessed and recognized under UV light illumination to protect personal private information. These invisible 2D barcodes provide a feasible solution for personal information protection and fit with a patient's privacy protection scenario very well, as we have demonstrated.