Bladder cancer hunting: A microfluidic paper-based analytical device.

Bladder cancer is the fourth most common cancer in men, and it is becoming a prevalent malignancy. Most of the regular clinical examinations are prompt evaluations with cystoscopy, renal function testing, which require high-precision instrument, well-trained operators, and high cost. In this study, a microfluidic paper-based analytical device (µPAD) was fabricated to detect nuclear matrix protein 22 (NMP22) and bladder cancer antigen (BTA) from the urine samples. Urine samples were collected from 11 bladder cancer patients and 10 well-beings as experiment and control groups, respectively, to verify the working efficiency of µPAD. A remarkable checkout efficiency of up to 90.91% was found from the results. Meanwhile, this method is feasible for home-based self-detection from urine samples within 10 min for the total process, which provides a new way for quick, economical, and convenient tumor diagnosis, prognosis evaluation, and drug response.

Non-Invasive On-Off Fluorescent Biosensor for Endothelial Cell Detection.

For rapid and convenient detection of living endothelial cells (ECs) specifically without immunostaining, we developed a biosensor based on turn-on fluorescent protein, named LV-EcpG. It includes a high-affinity peptide E12P obtained through phage display technology for specifically recognizing ECs and a turn-on EGFP fused with two linker peptides. The "on-off" switching mechanism of this genetically encoded fluorescent protein-based biosensor (FPB) ensured that fluorescence signals were activated only when binding with ECs, thus enabling these FPB characters for direct, visual, and non-invasive detection of ECs. Its specificity and multicolor imaging capability established LV-EcpG as a powerful tool for live EC research, with significant potential for diagnosing and treating cardiovascular diseases and tumor angiogenesis.

Loop-Mediated Isothermal Amplification (LAMP): Potential Point-of-Care Testing for Vulvovaginal Candidiasis.

PURPOSE: The aim of this study is to establish a loop-mediated isothermal amplification (LAMP) method for the rapid detection of vulvovaginal candidiasis (VVC). METHODS: We developed and validated a loop-mediated isothermal amplification (LAMP) method for detecting the most common Candida species associated with VVC, including C. albicans, N. glabratus, C. tropicalis, and C. parapsilosis. We evaluated the specificity, sensitivity, positive predictive value (PPV), negative predictive value (NPV), and Kappa value of the LAMP method to detect different Candida species, using the conventional culture method and internal transcribed spacer (ITS) sequencing as gold standards and smear Gram staining and real-time Rolymerase Chain Reaction (PCR) as controls. RESULTS: A total of 202 cases were enrolled, of which 88 were VVC-positive and 114 were negative. Among the 88 positive patients, the fungal culture and ITS sequencing results showed that 67 cases (76.14%) were associated with C. albicans, 13 (14.77%) with N. glabratus, 5 (5.68%) with C. tropicalis, and 3 (3.41%) with other species. Regarding the overall detection rate, the LAMP method presented sensitivity, specificity, PPV, NPV, and Kappa values of 90.91%, 100%, 100%, 93.4%, and 0.919, respectively. Moreover, the LAMP had a specificity of 100% for C. albicans, N. glabratus, and C. tropicalis, with a sensitivity of 94.03%, 100%, and 80%, respectively. Moreover, the microscopy evaluation had the highest sensitivity, while the real-time PCR was less specific for C. albicans than LAMP. In addition, CHROMagar Candida was inferior to LAMP in detecting non-albicans Candida (NAC) species. CONCLUSIONS: Based on the cost-effective, rapid, and inexpensive characteristics of LAMP, coupled with the high sensitivity and specificity of our VVC-associated Candida detection method, we provided a possibility for the point-of-care testing (POCT) of VVC, especially in developing countries and some laboratories with limited resources.

A Study on Improving the Efficacy of Nanoparticle-Based Photothermal Therapy: From Nanoscale to Micron Scale to Millimeter Scale.

Photothermal therapy based on nanoparticles is a promising method for cancer treatment. However, there are still many limits in practical application. During photothermal therapy, improving therapeutic effect is contradictory to reducing overheating in healthy tissues. We should make the temperature distribution more uniform and reduce the damage of healthy tissue caused by overheating. In the present work, we develop a simple computational method to analyze the temperature distribution during photothermal therapy at three levels (nanoscale, micron scale, and millimeter scale), and investigate the effects of nanoparticle size, volume fraction, light intensity, and irradiation shape on temperature distribution. We find that it is difficult to achieve good therapeutic effect just by adjusting the volume fraction of nanoparticles and light intensity. To achieve good therapeutic effect, we propose a new irradiation shape, spot array light. This method can achieve a better temperature distribution by easily regulating the positions of spots for the tumor with a large aspect ratio or a small one. In addition, the method of irradiation with spot array light can better reduce the overheating at the bottom and top of the tumor than the full-coverage light or others such as ring light. This theoretical work presents a simple method to investigate the effects of irradiation shape on therapy and provides a far more controlled way to improve the efficacy of photothermal therapy.

Doxorubicin-induced toxicity to 3D-cultured rat ovarian follicles on a microfluidic chip.

Doxorubicin (DOX) has dose-dependent toxicity on ovarian follicles (OFs), and the inhibition of different signaling molecules along with the DOX application for enhancing its efficacy can also upsurge this toxicity. Therefore, it is strongly required to explore the mechanism of DOX-induced toxicity in 3D culture systems for protecting the OFs. A microfluidic chip was used to culture a single OF to identify the potential signaling molecules and their combined effects on OFs dynamically. The chip offers better 3D biomimetic microenvironment to the growing OF than 2D culture systems. The OFs cultured on the chip were treated with DOX and the inhibitors of Src, Ca2+, and PIM. Their mutual effects were studied on OFs growth and 17ß-estradiol secretion. Besides, the RNA levels of B4GALT2 and UNC5C genes of DOX-exposed OFs were detected by RT-qPCR, and TUNEL staining experiments were conducted to check the OF apoptosis. The results showed that DOX application reduced the OFs growth and hormone secretion and induced apoptosis in the OFs. Moreover, the DOX-induced toxic effects were enriched by Src and PIM inhibition, while reduced by the ER-Ca2+ channel inhibitor. This study specifically demonstrates the synergistic effects of some signaling molecules on DOX-mediated cellular functions of OFs and demands some meditative measures to decipher this toxicity for supporting the female endocrine and reproductive functions.

[Controlled observation of the efficacy on motion sickness in the prevention and treatment with jianpixingnao needling therapy].

OBJECTIVE: To verify the efficacy of Jianpi-xingniao needling therapy on prevention and treatment of motion sickness. METHODS: Sixty volunteers of motion sickness were randomized into an acupuncture group and a delayed acupuncture group, 30 cases in each one. In the acupuncture group, acupuncture was given at Baihui (GV 20), Sishencong (EX-HN 1), Neiguan (PC 6), Zusanli (ST 36) and Hegu (LI 4). The needles were retained for 20 min. The treatment was given twice a week and 10 treatments were required. In the delayed acupuncture group, acupuncture was postponed, meaning no acupuncture during observation stage. Graybel scale was adopted to observe the score of symptoms and physical signs of the subjects of motion sickness before and after intervention. The efficacy was compared between the two groups. RESULTS: Twenty-five cases in the acupuncture group and 22 cases in the delayed acupuncture group were included in the statistical analysis. The score of symptoms and physical signs of motion sickness was reduced significantly after treatment in the acupuncture group as compared with that before treatment (10.12 +/- 3.37 vs 0.92 +/- 0.40, P < 0.05). The score in the acupuncture group was lower apparently than that in the delayed acupuncture group (0.92 +/- 0.40 vs 9.86 +/- 2.53, P < 0.05). The difference was not significant before and after treatment in the self-comparison of the delayed acupuncture group (P > 0.05). The total effective rate was 96.0% (24/25) in the acupuncture group, which was significantly better than 0.0% (0/22) in the delayed acupuncture group (P < 0.01). CONCLUSION: Jianpixingniao needling therapy relieves the symptoms of motion sickness in the patients and achieves a better clinical efficacy.