RESUMO
74 individuals of "cambute" (Strombus gracilior), were collected in Playa Panamá (10 degrees 35' 45"N, 85 degrees 40' 09" W), Costa Rica. The sex ratio was 1:1 and the size range 70-80mm (total length). The yield was 80% of edible meat. The average values of the macroconstituents to both sizes were: water 72.6% males and 70.9% females; 19.09g% protein in males and 26.89g% in females; carbohydrates 1.85g% males and 1.35g% females; 0.44g% crude fiber both males and females; within ashes there wre variations (2.30-3.71g%) among size and sex. Fat reached a maximum value of 0.94 and 0.91g% (length 80mm) for males and females respectively. In minerals, maximum values for the Na and K of 1440.88 and 1898.33 micrograms/g respectively were obtained for 80mm males; females of the same size showed values of 1376.97 micrograms/g for the Na and of 2393.09 micrograms/g for K; the other minerals (Cu, Mg, Fe) had lower values in relation to the above but the normal according to the literature.
Assuntos
Carne/análise , Valor Nutritivo , Frutos do Mar , Fatores Etários , Análise de Variância , Animais , Costa Rica , Feminino , MasculinoRESUMO
Complete electronic DNA profiles of 2006 randomly selected Costa Ricans, typed for 7 PCR-based loci, are presented. Such data may prove valuable for anthropological and forensic studies of the Costa Rican population.
Assuntos
Impressões Digitais de DNA , Genética Populacional , Reação em Cadeia da Polimerase/métodos , Costa Rica , Genótipo , HumanosRESUMO
Band 3 (AE1) is one of the most abundant proteins in the membrane of the human erythrocyte. This protein works as an anionic CI - and HCO3- exchanger and it also functions as an anchor for several proteins of the erythrocyte's cytoesqueleton. Several mutations have been described and many polymorphic variants have been associated to hereditary spherocytosis. The identification of a genetic marker at position 5' of the AEl gene could be associated to several molecular defects of the erythrocyte. This genetic marker is a restriction fragment length polymorphism (RFLP) produced by restriction enzime Pst I. For this polymorphism a total of 216 individuals belonging to seven different populations were analyzed: one from the Central Valley, two African descendants (Lim6n and Guanacaste) and four Amerindians (Bribri, Cabecar, Maleku and Guaymi). The most frequent allele in the Amerindian population was no 1. No significant differences were found with respect to the Hardy-Weinberg equilibrium in six of the populations, although the Guaymi group does present significative differences
Assuntos
Proteína 1 de Troca de Ânion do Eritrócito/genética , Frequência do Gene/genética , Genética Populacional , Polimorfismo Genético/genética , População Negra , Costa Rica , Marcadores Genéticos , Genótipo , Humanos , Indígenas Centro-Americanos/genética , Fenótipo , Polimorfismo de Fragmento de Restrição , População BrancaRESUMO
Nicaraguans have become the most numerous and fastest increasing minority in Costa Rica: at present they represent around 6% of the total population of the country. We have analyzed the allele and genotype frequencies of six PCR-based genetic markers (LDLR, GYPA, HBGG, D7S8, GC, and HLA-DQA1) in 100 unrelated Nicaraguans living in Costa Rica. All loci studied were in Hardy-Weinberg equilibrium. Some statistical parameters of forensic interest were also calculated (h, PD and CE). Allele frequencies of the markers HLA-DQA1 and GYPA were found to be significantly different between the populations of Nicaragua and Costa Rica. Nevertheless, genetic distances showed that Nicaragua is close to other Hispanic-admixed populations like those from Argentina, Chile, Colombia, Costa Rica, and USA Hispanics. The loci set was assessed to be useful for paternity testing and individual identification in the Nicaraguan population residing in Costa Rica.
Assuntos
Frequência do Gene , Marcadores Genéticos , Adulto , Alelos , Costa Rica , Feminino , Antropologia Forense , Genótipo , Humanos , Masculino , Nicarágua/etnologia , Paternidade , Reação em Cadeia da Polimerase , Polimorfismo GenéticoRESUMO
Complete electronic DNA profiles of 2006 randomly selected Costa Ricans, typed for 7 PCR-based loci, are presented. Such data may prove valuable for anthropological and forensic studies of the Costa Rican population.
Assuntos
Humanos , Genética Populacional , Impressões Digitais de DNA , Reação em Cadeia da Polimerase/métodos , Costa Rica , GenótipoRESUMO
Band 3 (AE1) is one of the most abundant proteins in the membrane of the human erythrocyte. This protein works as an anionic CI - and HCO3- exchanger and it also functions as an anchor for several proteins of the erythrocyte's cytoesqueleton. Several mutations have been described and many polymorphic variants have been associated to hereditary spherocytosis. The identification of a genetic marker at position 5' of the AEl gene could be associated to several molecular defects of the erythrocyte. This genetic marker is a restriction fragment length polymorphism (RFLP) produced by restriction enzime Pst I. For this polymorphism a total of 216 individuals belonging to seven different populations were analyzed: one from the Central Valley, two African descendants (Lim6n and Guanacaste) and four Amerindians (Bribri, Cabecar, Maleku and Guaymi). The most frequent allele in the Amerindian population was no 1. No significant differences were found with respect to the Hardy-Weinberg equilibrium in six of the populations, although the Guaymi group does present significative differences.