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1.
Nature ; 432(7019): 910-3, 2004 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-15602564

RESUMO

Since the recognition of prokaryotes as essential components of the oceanic food web, bacterioplankton have been acknowledged as catalysts of most major biogeochemical processes in the sea. Studying heterotrophic bacterioplankton has been challenging, however, as most major clades have never been cultured or have only been grown to low densities in sea water. Here we describe the genome sequence of Silicibacter pomeroyi, a member of the marine Roseobacter clade (Fig. 1), the relatives of which comprise approximately 10-20% of coastal and oceanic mixed-layer bacterioplankton. This first genome sequence from any major heterotrophic clade consists of a chromosome (4,109,442 base pairs) and megaplasmid (491,611 base pairs). Genome analysis indicates that this organism relies upon a lithoheterotrophic strategy that uses inorganic compounds (carbon monoxide and sulphide) to supplement heterotrophy. Silicibacter pomeroyi also has genes advantageous for associations with plankton and suspended particles, including genes for uptake of algal-derived compounds, use of metabolites from reducing microzones, rapid growth and cell-density-dependent regulation. This bacterium has a physiology distinct from that of marine oligotrophs, adding a new strategy to the recognized repertoire for coping with a nutrient-poor ocean.


Assuntos
Adaptação Fisiológica/genética , Genoma Bacteriano , Plâncton/genética , Plâncton/fisiologia , Roseobacter/genética , Roseobacter/fisiologia , Água do Mar/microbiologia , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Genes Bacterianos/genética , Biologia Marinha , Dados de Sequência Molecular , Oceanos e Mares , Filogenia , Plâncton/classificação , RNA Ribossômico 16S/genética , Roseobacter/classificação
2.
Genome Res ; 15(9): 1284-91, 2005 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16109971

RESUMO

Rice (Oryza sativa L.) chromosome 3 is evolutionarily conserved across the cultivated cereals and shares large blocks of synteny with maize and sorghum, which diverged from rice more than 50 million years ago. To begin to completely understand this chromosome, we sequenced, finished, and annotated 36.1 Mb ( approximately 97%) from O. sativa subsp. japonica cv Nipponbare. Annotation features of the chromosome include 5915 genes, of which 913 are related to transposable elements. A putative function could be assigned to 3064 genes, with another 757 genes annotated as expressed, leaving 2094 that encode hypothetical proteins. Similarity searches against the proteome of Arabidopsis thaliana revealed putative homologs for 67% of the chromosome 3 proteins. Further searches of a nonredundant amino acid database, the Pfam domain database, plant Expressed Sequence Tags, and genomic assemblies from sorghum and maize revealed only 853 nontransposable element related proteins from chromosome 3 that lacked similarity to other known sequences. Interestingly, 426 of these have a paralog within the rice genome. A comparative physical map of the wild progenitor species, Oryza nivara, with japonica chromosome 3 revealed a high degree of sequence identity and synteny between these two species, which diverged approximately 10,000 years ago. Although no major rearrangements were detected, the deduced size of the O. nivara chromosome 3 was 21% smaller than that of japonica. Synteny between rice and other cereals using an integrated maize physical map and wheat genetic map was strikingly high, further supporting the use of rice and, in particular, chromosome 3, as a model for comparative studies among the cereals.


Assuntos
Cromossomos de Plantas/genética , Oryza/genética , Poaceae/genética , Arabidopsis/genética , Mapeamento Cromossômico , Cromossomos Artificiais Bacterianos/genética , Genes de Plantas , Repetições Minissatélites , Dados de Sequência Molecular , Oryza/classificação , Mapeamento Físico do Cromossomo , Poaceae/classificação , Proteoma , Especificidade da Espécie , Zea mays/classificação , Zea mays/genética
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