Leaching characteristics and hazard evaluation of bottom ash generated from common biomedical waste incinerators.

India has more than 202 biomedical waste incinerators, however, knowledge on the chemical characteristics of incinerator ash is lacking. The objective of this study was to evaluate the lecahablility characteristics of bottom ash and to study the levels of incineration by-products viz. polycyclic aromatic hydrocarbons (PAHs), and polychlorinated biphenyls (PCBs). Bottom ash samples from 13 common biomedical waste treatment facilities (CBMWTF) were colleted and subjected to leachig test, sequential extraction procedure (SEP) and PAHs and PCBs analysis. Among metals, cadmium, chromium, manganese, lead and zinc were found higher than the regulatory limits indicating its hazardous nature. SEP showed that substantial fraction of Cd (30%) and Zn (25%) were associated with leachable fractions, whereas metals such as Cr, Fe, Mn, and Ni were mainly associated with reducible, organics and residual fractions. Concentrations of USEPA 16 priority PAHs ranged between 0.17-12.67 mg kg-1 and the total toxic equivalents (TEQ) were in the range of 0.9-421.9 ng TEQ/g. PAHs with 4-rings dominated all the samples and accounted for 68% to total PAHs concentrations. Concentration of Σ19 PCB congeners ranged from 420.4 to 724.3 µg kg-1. PCBs homologue pattern was dominated by mono- to tetra chlorinated congeners (60-86%). The findings indicate the need for segregation of plastics from biomedical waste, improvement of combustion efficiency, and efficient air pollution control devices for the existing incinerators in CBMWTFs.

Enrichment and isolation of endosulfan degrading and detoxifying bacteria.

In the present study, degradation of endosulfan by a mixed culture isolated from a pesticide-contaminated soil was studied in batch experiments. After two weeks of incubation, the mixed culture was able to degrade 73% and 81% of alpha and beta endosulfan respectively. Endodiol was identified by GC/MS as degradation intermediate. The toxicity studies of endosulfan before and after degradation were carried out using micronucleus assay on human polymorphonuclear cells. The findings suggested that the metabolism of endosulfan isomers by the mixed culture was accompanied by significant reduction in the toxicity. Studies were also carried out to quantify the degradation potential of the individual species in the mixed bacterial culture. Two cultures identified by 16S rRNA as Stenotrophomonas maltophilia and Rhodococcus erythropolis were found to be responsible for majority of the degradation by the mixed culture. S. maltophilia showed better degradation efficiency compared to that by R. erythropolis. This is the first report of endosulfan degradation using the above-mentioned organisms.