RESUMO
OBJECTIVES: Maternal health behaviours (MHBs) can influence pregnancy outcomes. Despite efforts internationally to encourage positive MHBs, women often fail to comply with pregnancy guidelines. International studies show differences in MHBs between nationalities and an effect of time spent in the host country. There is limited Irish data in this area, with no previous research relating to the effect of time in Ireland. STUDY DESIGN: This study is a cross-sectional analysis of the Growing Up in Ireland infant cohort, a nationally representative longitudinal study. METHODS: Examination of the MHBs of non-Irish nationals during pregnancy and the effect of time in Ireland on the said behaviours. RESULTS: An association was found between time spent in Ireland and increased alcohol consumption prevalence. Those living in Ireland for ≤5 years were 60.8% less likely to consume alcohol during pregnancy (0.000) and 29.3% less likely to take folic acid before conception (0.021). Those who smoked during pregnancy were 98.6% more likely to consume alcohol (0.000) and those who consumed alcohol were 95.2% more likely to smoke during pregnancy (0.000). CONCLUSIONS: The results demonstrate differences in MHBs and the influence of time living in Ireland. These findings are of relevance for policy and intervention planning to optimise pregnancy outcomes among non-nationals.
Assuntos
Emigrantes e Imigrantes/psicologia , Emigração e Imigração/estatística & dados numéricos , Comportamentos Relacionados com a Saúde , Gestantes/psicologia , Aculturação , Adulto , Consumo de Bebidas Alcoólicas/epidemiologia , Estudos Transversais , Emigrantes e Imigrantes/estatística & dados numéricos , Feminino , Ácido Fólico , Humanos , Irlanda/epidemiologia , Estudos Longitudinais , Gravidez , Resultado da Gravidez , Prevalência , Fumar/epidemiologia , Fatores de TempoRESUMO
NHS Blood and Transplant Tissue and Eye Services banks and issues, cut, shaped and washed bone from deceased donors. The bone is cut/shaped prior to washing and then processed to remove up to 99.9% of blood, bone marrow and associated cells. The processed bone is then sterilised by gamma irradiation with or without a freeze-drying step. Removal of donor blood and bone marrow has been reported to aid incorporation of allograft bone without affecting the biomechanical properties of the bone. However, cut and shaped bone is not suitable for some orthopaedic procedures and some orthopaedic surgeons do not wish to use irradiated bone. Therefore, Tissue and Eye Services have also developed a method for washing intact femoral head bone, from living and deceased donors. We have observed that processing of intact femoral head bone does not always result in removal of 99% (or above) of marrow components and can be as low as 93% removal. We have examined washed femoral head bone and found the presence of internal fluid-filled cysts within subchondral cancellous bone in bone from living donors. The cysts have been identified as geodes and we suggest that these geodes may be responsible for the reduction in bone marrow component removal in living donor bone during processing.
Assuntos
Medula Óssea/patologia , Cistos/patologia , Cabeça do Fêmur/patologia , Doadores de Tecidos , Adulto , Idoso , Idoso de 80 Anos ou mais , DNA/isolamento & purificação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas/isolamento & purificaçãoRESUMO
This study was carried out to investigate leakage/transport across the bag material of six outer cryopreservation bags in common use within NHS Blood and Transplant. In order to do this two different leak testing procedures; coloured dye and hydrogen tracer gas, were used. The data obtained show that a coloured dye cannot permeate through the materials both at room temperature and following storage at liquid nitrogen temperature (- 196 °C). In addition, when filled with the smallest elemental molecule, hydrogen, in the form of a tracer gas, all of the bags only allowed trace amounts of hydrogen to escape, either through the seal or the bag material. The data indicated that each of the bag materials tested would be capable of preventing bacterial or viral cross-contamination as long as the material remained intact.
Assuntos
Armazenamento de Sangue , Preservação de Sangue , Criopreservação , Embalagem de Medicamentos , Armazenamento de Sangue/métodos , Preservação de Sangue/instrumentação , Preservação de Sangue/métodos , Corantes/análise , Criopreservação/instrumentação , Criopreservação/métodos , Embalagem de Medicamentos/instrumentação , Embalagem de Medicamentos/métodos , Desenho de Equipamento , Humanos , Hidrogênio/análise , Permeabilidade , Embalagem de Produtos , TemperaturaRESUMO
The aims of this study were to develop a biological large diameter vascular graft by decellularisation of native human aorta to remove the immunogenic cells whilst retaining the essential biomechanical, and biochemical properties for the ultimate benefit of patients with infected synthetic grafts. Donor aortas (n = 6) were subjected to an adaptation of a propriety decellularisation process to remove the cells and acellularity assessed by histological analysis and extraction and quantification of total DNA. The biocompatibility of the acellular aortas was determined using standard contact cytotoxicity tests. Collagen and denatured collagen content of aortas was determined and immunohistochemistry was used to determine the presence of specific extracellular matrix proteins. Donor aortas (n = 6) were divided into two, with one half subject to decellularisation and the other half retained as native tissue. The native and decellularised aorta sections were then subject to uniaxial tensile testing to failure [axial and circumferential directions] and suture retention testing. The data was compared using a paired t-test. Histological evaluation showed an absence of cells in the treated aortas and retention of histoarchitecture including elastin content. The decellularised aortas had less than 15 ng mg-1 total DNA per dry weight (mean 94% reduction) and were biocompatible as determined by in vitro contact cytotoxicity tests. There were no gross changes in the histoarchitecture [elastin and collagen matrix] of the acellular aortas compared to native controls. The decellularisation process also reduced calcium deposits within the tissue. The uniaxial tensile and suture retention testing revealed no significant differences in the material properties (p > 0.05) of decellularised aorta. The decellularisation procedure resulted in minimal changes to the biological and biomechanical properties of the donor aortas. Acellular donor aorta has excellent potential for use as a large diameter vascular graft.
Assuntos
Aorta/química , Aorta/ultraestrutura , Bioprótese , Prótese Vascular , Alicerces Teciduais/química , Células A549 , Aorta/citologia , Materiais Biocompatíveis/química , Fenômenos Biomecânicos , Colágeno/análise , DNA/análise , Matriz Extracelular/química , Matriz Extracelular/ultraestrutura , Humanos , Teste de Materiais , Resistência à Tração , Engenharia Tecidual/métodosRESUMO
Decellularised tissue allografts have been used in reconstructive surgical applications and transplantation for many years. Some of the current methods of sterilisation have a detrimental effect on the tissue graft structure and function. The anti-microbial activity of cupric ions and hydrogen peroxide (H2O2) are well known however their combined application is not currently utilised as a decontamination agent in the tissue banking world sector. The aim of this study was to determine the combined concentrations of copper chloride (CuCl2) and H2O2 that have the optimal bactericidal and sporicidal activity on decellularised (dCELL) human dermis. The first part of this study established the decimal reduction time (D-value) of CuCl2 (0.1 mg/L and 1 mg/L) together with H2O2 (0.01, 0.1, 0.5 and 1%) for Staphylococcus epidermidis, Escherichia coli and Bacillus subtilis spores. The second part of this study identified the most effective CuCl2 and H2O2 concentration that decontaminated dCELL human dermis inoculated with these pathogens. Of all the concentrations tested, 0.1 mg/L CuCl2 in combination with 1% H2O2 had the shortest D-value; S. epidermidis D = 3.15 min, E. coli D = 2.62 min and B. subtilis spores D = 18.05 min. However when adsorbed onto dCELL dermis, S. epidermidis and E. coli were more susceptible to 1 mg/L CuCl2 together with 0.5% H2O2. These studies show promise of CuCl2-H2O2 formulations as potential sterilants for decellularised dermal allografts.
Assuntos
Aloenxertos/efeitos dos fármacos , Antibacterianos/farmacologia , Peróxido de Hidrogênio/farmacologia , Esterilização , Descontaminação/métodos , Escherichia coli/patogenicidade , Humanos , Esporos Bacterianos/efeitos dos fármacos , Esterilização/métodosRESUMO
NHS Blood and Transplant Tissue and Eye Services (TES) and Scottish National Blood Transfusion Services Tissues and Cells Directorate (TCD) currently bank whole, frozen femoral head bone from living donors who are undergoing primary hip replacement surgery. When required, the bone is issued to a surgeon still frozen on dry ice (- 79 °C). Consequently, the femoral head bone is not processed, is not sterilised and at the time of issue, it contains donor blood, bone marrow and associated cells. We have previously shown that, cut, shaped and washed bone from deceased donors can be processed to remove up to 99.9% of blood, bone marrow and associated cells (Eagle et al. 2015). However, cut and shaped bone is not suitable for some orthopaedic procedures and some orthopaedic surgeons do not wish to use irradiated bone; therefore in this report, a method has been developed in which whole femoral heads can be washed to remove donor blood and bone marrow components. Processing results in excess of 99% bone marrow component removal-soluble protein, haemoglobin and DNA; the procedure is performed inside a closed system, thereby eliminating the need for terminal sterilisation by irradiation. In addition, uniaxial testing demonstrated no difference in compressive strength between washed and unwashed bone. We suggest that this washed bone may be capable of improving incorporation after grafting without disturbing biomechanical properties of the graft.
Assuntos
Artroplastia de Quadril , Transplante Ósseo/instrumentação , Cabeça do Fêmur/citologia , Doadores Vivos , Esterilização , Adulto , Artroplastia de Quadril/instrumentação , Artroplastia de Quadril/métodos , Transplante Ósseo/métodos , DNA , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Esterilização/instrumentação , Transplante Homólogo/instrumentação , Transplante Homólogo/métodosRESUMO
In the "liquidus tracking" (LT) approach to cryopreservation both the temperature and the concentration of cryoprotectant (CPA) are controlled such that solution composition "tracks" the liquidus (melting point) line for that system. Ice crystal formation is prevented but the tissue is not exposed to CPA concentrations exceeding those experienced by cells during conventional cryopreservation. This approach is particularly appropriate for articular cartilage because chondrocytes in situ are exquisitely susceptible to damage by the crystallisation of ice. This project aimed to develop a suitable process for tissue to be used in the surgical repair of damaged human knee joints. A high proportion of the chondrocytes should be alive. Human articular cartilage was obtained from deceased donors and dimethyl sulphoxide (DMSO) was used as the CPA, cooling was at 0.14°C/min and warming at 0.42°C/min. The vehicle solution was CPTes2. A program of increasing DMSO concentration was developed for cooling and this gave satisfactory tissue concentrations but reduction of DMSO concentration during warming was inadequate, resulting in higher tissue concentrations than required. Biomechanical testing indicated a compressive modulus of 9.5±1.3 MPa in LT-processed cartilage, with control values of 11.6±0.8 MPa (p>0.05, Student's t-test). Measurement of GAG synthesis sometimes approached 65% or 85% of control, but the variability of replicate data prevented firm conclusions. Ideally allograft tissue should score 1A or above on the Noyes scale and the donor age should be less than 46 years but the cartilage used in this study did not meet these standards.
Assuntos
Aloenxertos/cirurgia , Cartilagem Articular/cirurgia , Criopreservação/métodos , Articulação do Joelho/cirurgia , Adulto , Idoso , Condrócitos/fisiologia , Crioprotetores/farmacologia , Cristalização , Dimetil Sulfóxido/farmacologia , Feminino , Humanos , Traumatismos do Joelho/cirurgia , Masculino , Pessoa de Meia-Idade , TemperaturaRESUMO
Innate B and T lymphocytes are a subset of lymphocytes that express a restricted set of semi-invariant, germ-line-encoded, autoreactive antigen receptors. Although they have long been set apart from mainstream immunological thought, they now seem to represent a distinct immune-recognition strategy that targets conserved stress-induced self-structures, rather than variable foreign antigens. Innate lymphocytes regulate a range of infectious, tumour and autoimmune conditions. New studies have shed light on the principles and mechanisms that drive their unique development and function, and show their resemblance to another subset of innate lymphocytes, the natural killer cells.
Assuntos
Autoimunidade , Subpopulações de Linfócitos B/imunologia , Subpopulações de Linfócitos T/imunologia , Animais , Autoantígenos , Humanos , Modelos Imunológicos , Receptores de Antígenos de Linfócitos B/metabolismo , Receptores de Antígenos de Linfócitos T/metabolismo , Tolerância a Antígenos PrópriosRESUMO
Human tissue is shipped to surgeons in the UK in either a freeze-dried or frozen state. To ensure quality and safety of the tissue, frozen tissue must be shipped in insulated containers such that tissue is maintained at an appropriate temperature. UK Blood Transfusion Service regulations state "Transportation systems must be validated to show maintenance of the required storage temperature" and also state that frozen, non-cryopreserved tissue "must be transported at -20 °C or lower" (Guidelines for the Blood Transfusion Services in the United Kingdom, 8th Edn. 2013). To maintain an expiry date for frozen tissue longer than 6 months, the tissue must be maintained at a temperature of -40 °C or below. The objective of this study was to evaluate and validate the capability of a commercially available insulated polystyrene carton (XPL10), packed with dry ice, to maintain tissue temperature below -40 °C. Tissue temperature of a single frozen femoral head or a single frozen Achilles tendon, was recorded over a 4-day period at 37 °C, inside a XPL10 carton with dry ice as refrigerant. The data demonstrate that at 37 °C, the XPL10 carton with 9.5 kg of dry ice maintained femoral head and tendon tissue temperature below -55 °C for at least 48 h; tissue temperature did not rise above -40 °C until at least 70 h. Data also indicated that at a storage temperature lower than 37 °C, tissue temperature was maintained for longer periods.
Assuntos
Temperatura Corporal/fisiologia , Criopreservação/métodos , Embalagem de Produtos/métodos , Tendões/fisiologia , Bancos de Tecidos/normas , Meios de Transporte/instrumentação , Criopreservação/instrumentação , Criopreservação/normas , Gelo-Seco , Desenho de Equipamento , Análise de Falha de Equipamento , Cabeça do Fêmur/fisiologia , Cabeça do Fêmur/transplante , Humanos , Masculino , Pessoa de Meia-Idade , Política Organizacional , Embalagem de Produtos/normas , Tendões/transplante , Meios de Transporte/métodos , Reino UnidoRESUMO
Demineralised bone matrix (DBM) is produced by grinding cortical bone into a powder, sieving the powder to obtain a desired size range and then demineralising the powder using acid. Protocols for the production of DBM powder have been published since 1965 and the powder can be used in lyophilised form or it can be mixed with a carrier to produce a paste or putty. The powder is generally produced from cortical bone which has been processed to remove blood, bone marrow and bone marrow components, including fat. Removal of fat is accomplished by incorporating incubation in an organic solvent, often chloroform, chloroform/methanol or acetone. The use of organic solvents in a clean room environment in a human tissue bank is problematic and involves operator exposure and the potential for the solvent to be trapped in air filters or recirculated throughout the clean room suite. Consequently, in this study, we have developed a cortical bone washing step which removes fat/lipid without the use of an organic solvent. Bone was prepared from six femoral shafts from three donors by dissecting soft tissue and bisecting the shaft, the shafts were then cut into ~9-10 cm lengths. These struts were then taken through a series of hot water washes at 56-59 °C, centrifugation and decontamination steps. Washed cortical struts were then lyophilised before being ground with a compressed air milling machine. The ground bone was sieved, demineralised, freeze-dried and terminally sterilised with a target dose of 25 kGy gamma irradiation. The DBM powder was evaluated for residual calcium content, in vitro cytotoxicity and osteoinductivity by implantation into the muscle of an athymic mouse. Data indicated that in addition to removing in excess of 97% DNA and extractable soluble protein, the washing protocol reduced lipid 10,000-fold. The processed bone was easily ground without clogging the grinder; the sterilised DBM powder was not cytotoxic but was osteoinductive in the animal model. Therefore, we have developed a method of producing osteoinductive DBM without the need to use organic solvents.
Assuntos
Técnica de Desmineralização Óssea/métodos , Desenvolvimento Ósseo/efeitos dos fármacos , Matriz Óssea/química , Substitutos Ósseos/administração & dosagem , Substitutos Ósseos/síntese química , Osteogênese/efeitos dos fármacos , Adulto , Idoso , Animais , Humanos , Masculino , Teste de Materiais , Camundongos , Camundongos Nus , Pessoa de Meia-Idade , Compostos Orgânicos/química , Pós , Solventes/químicaRESUMO
NHSBT Tissue Services issues bone to surgeons in the UK in two formats, fresh-frozen unprocessed bone from living donors and processed bone from deceased donors. Processed bone may be frozen or freeze dried and all processed bone is currently subjected to a washing protocol to remove blood and bone marrow. In this study we have improved the current bone washing protocol for cancellous bone and assessed the success of the protocol by measuring the removal of the bone marrow components: soluble protein, DNA and haemoglobin at each step in the process, and residual components in the bone at the end of the process. The bone washing protocol is a combination of sonication, warm water washes, centrifugation and chemical (ethanol and hydrogen peroxide) treatments. We report that the bone washing protocol is capable of removing up to 99.85 % soluble protein, 99.95 % DNA and 100 % of haemoglobin from bone. The new bone washing protocol does not render any bone cytotoxic as shown by contact cytotoxicity assays. No microbiological cell growth was detected in any of the wash steps. This process is now in use for processed cancellous bone issued by NHSBT.
Assuntos
Osso e Ossos , Cadáver , Desinfecção , Doadores de Tecidos , DNA/análise , Hemoglobinas/análise , HumanosRESUMO
Many of the decellularised dermis products on the market at present are aspectically produced. NHS Blood and Transplant Tissue Services have developed a method of producing a dCELL human dermis which has been terminally sterilised by gamma irradiation. The terminally sterilised decellularised dermis was compared with cellular tissue and examined for histology, residual DNA content, biomechanical and biochemical properties, in vitro cytotoxicity and in vivo implantation in a mouse model. No alterations in morphology as viewed by light microscopy were observed and DNA removal was 99%. There were no significant changes in ultimate tensile stress or evidence for collagen denaturation or cytotoxicity. The in vivo studies did not indicate any adverse tissue reactions in the mouse model and demonstrated incorporation of dCELL human dermis into the host. Decellularisation, followed by terminal sterilisation with gamma irradiation, is an appropriate method to produce a human dermis allograft material suitable for transplantation.
Assuntos
Derme Acelular/microbiologia , Colágeno/metabolismo , Derme/fisiologia , Derme/transplante , Esterilização/métodos , Engenharia Tecidual/métodos , Animais , Derme/microbiologia , Módulo de Elasticidade , Teste de Materiais , Camundongos , Resistência à TraçãoRESUMO
Osteochondral allografting techniques are limited by the availability of suitable donor tissue; there is an urgent need for effective cryopreservation. A fundamental requirement is the need to establish initial conditions of exposure to cryoprotectant that the chondrocytes will tolerate and that load the tissue with an adequate concentration of cryoprotectant. Three vehicle solutions to transport DMSO into the tissue were studied. Knee joints were obtained from deceased donors with appropriate consent. Whole condyles were treated with 20% w/w DMSO in each of three vehicle solutions and chondrocyte function and tissue CPA content measured. The results showed that exposure to 20% DMSO in each vehicle solution for 2 hours at 0 degrees C was tolerated without loss of GAG synthetic activity. It was observed that penetration of DMSO increased little after 1 hour of CPA exposure at 0 degrees C but the final tissue concentration of CPA was markedly lower than that in the medium.
Assuntos
Cartilagem Articular/metabolismo , Condrócitos/efeitos dos fármacos , Crioprotetores/farmacocinética , Dimetil Sulfóxido/farmacocinética , Adulto , Transporte Biológico , Cartilagem Articular/citologia , Cartilagem Articular/efeitos dos fármacos , Condrócitos/citologia , Condrócitos/metabolismo , Criopreservação , Crioprotetores/administração & dosagem , Crioprotetores/farmacologia , Dimetil Sulfóxido/administração & dosagem , Dimetil Sulfóxido/farmacologia , Humanos , Veículos Farmacêuticos/químicaRESUMO
The aim of the study was to determine the added value of stroke protocol MRI following negative initial CT brain in the acute stroke setting. A retrospective study was performed over a 6 month period in a tertiary referral stroke centre. Patients were selected from the stroke and radiology databases. Inclusion criteria: clinical stroke syndrome, negative initial CT with subsequent MRI study with diffusion weighted sequences. Ninety two patients were reviewed and 73 (M:F of 39:34, mean age 62.1 ± 14.0 years) met the inclusion criteria. Twenty MRI studies (27.4%) were positive for acute/subacute ischaemia in the setting of a normal initial CT. The average time interval between initial CT and MRI brain imaging was 4.7 ± 2.6 days. Whilst CT continues to be the first line imaging investigation for acute stroke, MRI has substantial added value following negative initial CT in the diagnosis of stroke.
Assuntos
Imagem de Difusão por Ressonância Magnética/estatística & dados numéricos , Acidente Vascular Cerebral/diagnóstico , Idoso , Protocolos Clínicos , Reações Falso-Negativas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Atenção Terciária à Saúde/estatística & dados numéricos , Tomografia Computadorizada por Raios XRESUMO
OBJECTIVE: To examine the extent to which early child nutrition, maternal antenatal lifestyle behaviours and child diet and lifestyle explain social class inequalities in the risk of rapid weight gain between birth and 3 years and obesity at age 3 years. DESIGN: A longitudinal and prospective birth cohort study. SUBJECTS: Nationally representative sample of 11,134 children and their parents followed from 9 months of age until 3 years. Child weight and maternal height and weight were measured at 9 months and 3 years and child birth weight was extracted from hospital records. Other predictors of child growth and obesity were collected by maternal report at 9 months and 3 years. RESULTS: Although born lighter on average, children of unskilled manual parents were 274 g heavier than children of professional parents by 3 years of age. The fully adjusted model of rapid growth from birth to 3 years of age and obesity at 3 years of age accounted for all social class differentials. Breastfeeding and age at the introduction of solids were associated with the largest average reduction (41%) in the odds ratio (OR) of rapid growth in the first 9 months of life for each class relative to the professional class. In the period from 9 months to 3 years of age, the class differential in rapid growth was reduced most by measures of the child's diet and lifestyle. However, the impact of the groups of predictors varied by social class. For early life growth, among the non-manual classes the proportionate reductions are largest when adjusted for early infant nutrition, whereas maternal prenatal smoking is more important for the manual social classes. CONCLUSION: Preventative interventions to reduce levels of childhood obesity should be multi-dimensional but different dimensions should be given more or less significance depending on socio-economic group.
Assuntos
Aleitamento Materno/estatística & dados numéricos , Comportamento Materno , Obesidade Infantil/epidemiologia , Fumar/epidemiologia , Classe Social , Aumento de Peso , Adulto , Análise de Variância , Peso ao Nascer , Índice de Massa Corporal , Pré-Escolar , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Lactente , Estudos Longitudinais , Masculino , Razão de Chances , Obesidade Infantil/prevenção & controle , Estudos Prospectivos , Fatores de Risco , DesmameRESUMO
UNLABELLED: Indoor fine particles (FPs) are a combination of ambient particles that have infiltrated indoors, and particles that have been generated indoors from activities such as cooking. The objective of this paper was to estimate the infiltration factor (Finf ) and the ambient/non-ambient components of indoor FPs. To do this, continuous measurements were collected indoors and outdoors for seven consecutive days in 50 non-smoking homes in Halifax, Nova Scotia in both summer and winter using DustTrak (TSI Inc) photometers. Additionally, indoor and outdoor gravimetric measurements were made for each 24-h period in each home, using Harvard impactors (HI). A computerized algorithm was developed to remove (censor) peaks due to indoor sources. The censored indoor/outdoor ratio was then used to estimate daily Finfs and to determine the ambient and non-ambient components of total indoor concentrations. Finf estimates in Halifax (daily summer median = 0.80; daily winter median = 0.55) were higher than have been reported in other parts of Canada. In both winter and summer, the majority of FP was of ambient origin (daily winter median = 59%; daily summer median = 84%). Predictors of the non-ambient component included various cooking variables, combustion sources, relative humidity, and factors influencing ventilation. This work highlights the fact that regional factors can influence the contribution of ambient particles to indoor residential concentrations. PRACTICAL IMPLICATIONS: Ambient and non-ambient particles have different risk management approaches, composition, and likely toxicity. Therefore, a better understanding of their contribution to the indoor environment is important to manage the health risks associated with fine particles (FPs) effectively. As well, a better understanding of the factors Finf can help improve exposure assessment and contribute to reduced exposure misclassification in epidemiologic studies.
Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar em Ambientes Fechados/análise , Exposição Ambiental/análise , Monitoramento Ambiental/métodos , Material Particulado/análise , Habitação , Humanos , Nova Escócia , Estações do Ano , Inquéritos e Questionários , População UrbanaRESUMO
Application of a high-level decontamination or sterilisation procedure and cell removal technique to tendon allograft can reduce the concerns of disease transmission, immune reaction, and may improve remodelling of the graft after implantation. The decellularised matrix can also be used as a matrix for tendon tissue engineering. One such sterilisation factor, Peracetic acid (PAA) has the advantage of not producing harmful reaction residues. The aim of this study was to evaluate the effects of PAA treatment and a cell removal procedure on the production of tendon matrix. Human patellar tendons, thawed from frozen were treated respectively as: Group 1, control with no treatment; Group 2, sterilised with PAA (0.1 % (w/v) PAA for 3 h) Group 3, decellularised (incubation successively in hypotonic buffer, 0.1 % (w/v) sodium dodecyl sulphate, and a nuclease solution); Group 4, decellularised and PAA sterilised. Histological analysis showed that no cells were visible after the decellularisation treatment. The integrity of tendon structure was maintained after decellularisation and PAA sterilisation, however, the collagen waveform was slightly loosened. No contact cytotoxicity was found in any of the groups. Determination of de-natured collagen showed no significant increase when compared with the control. This suggested that the decellularisation and sterilisation processing procedures did not compromise the major properties of the tendon. The sterilised, decellularised tendon could be suitable for clinical use.
Assuntos
Aloenxertos/crescimento & desenvolvimento , Esterilização/métodos , Tendões/crescimento & desenvolvimento , Engenharia Tecidual/métodos , Aloenxertos/citologia , Aloenxertos/efeitos dos fármacos , Materiais Biocompatíveis/farmacologia , Morte Celular/efeitos dos fármacos , Linhagem Celular , Colágeno/metabolismo , Humanos , Desnaturação Proteica/efeitos dos fármacos , Solubilidade , Tendões/citologia , Tendões/efeitos dos fármacosRESUMO
The objective of this study was to design and test a protocol for the validation of banking methodologies for arterial allografts. A series of in vitro biomechanical and biological assessments were derived, and applied to paired fresh and banked femoral arteries. The ultimate tensile stress and strain, suture pullout stress and strain, expansion/rupture under hydrostatic pressure, histological structure and biocompatibility properties of disinfected and cryopreserved femoral arteries were compared to those of fresh controls. No significant differences were detected in any of the test criteria. This validation protocol provides an effective means of testing and validating banking protocols for arterial allografts.