RESUMO
Isoelectric focussing in a stable pH-gradient of ampholines, serum and fraction 4-4, containing hepatitis B antigen demonstrated that the antigen occupied the pI 4.31--6.03 zone with the maximum activity at pI 4.31--5.56. The activity of antibodies to the hepatitis B antigen was revealed in a donor serum and in the gamma-globulin fraction in the pI 7.76--8.4 zone with the activity maximum at pI 8.03--8.23. It was demonstrated immunochemically that antibodies against hepatitis B antigen belonged to immunoglobulin G.
Assuntos
Anticorpos Antivirais/análise , Antígenos da Hepatite B/análise , Humanos , Imunoquímica , Imunoglobulina G , Focalização Isoelétrica , gama-Globulinas/análiseRESUMO
Immunochemical analysis in combination with gel filtration and isoelectric focusing made it possible to state that in blood serum of healthy people 81.3 +/- 0.5% of administered trypsin is bound with alpha 1-antitrypsin and 18.7 +/- 0.6%--with alpha 2-macroglobulin. The latter is functionally heterogeneous, only 40% of it is bound with trypsin and in the formed complex the antigenic properties of trypsin and alpha 2-macroglobulin are lost. A great number of blood serum alpha 1-antitrypsin cannot fix trypsin. The content of such alpha 1-antitrypsin rises sharply with pathology available. In the immunochemical estimation of the organism inhibitory potential relative to proteolytic enzymes not only the amount of the inhibitor but also its functional activity should be taken into account. The data of immunochemical research of the blood serum isoelectrophoregrams show that the most considerable changes under conditions of pathology occur in alpha 2-macroglobulin.
Assuntos
Tripsina/metabolismo , alfa 1-Antitripsina/metabolismo , alfa-Macroglobulinas/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Cinética , Valores de Referência , alfa 1-Antitripsina/isolamento & purificação , alfa-Macroglobulinas/isolamento & purificaçãoRESUMO
By means of radial immunodiffusion the quantitative content of blood serum proteins was studied in healthy persons and in patients with the following impairments:gastric ulcer, duodenal ulcer, carcinoma of the stomach, disease of the operated stomach, hepatic echinococcosis. In blood sera of healthy persons the patterns of the proteins studied were found within the limits of normal values reported by other investigators. As compared with the normal values distinct quantitative alterations were observed in content of proteins under the above-mentioned pathological states.