RESUMO
An enantiomeric pair of C2-chiral bifunctionalised spin labels having a pyrrolidine nitroxide moiety, whose configurations were determined by X-ray crystal diffraction analysis, was prepared and applied to troponin C whose binding mode of double disulfide linkage was proved by EPR spectroscopy.
Assuntos
Carbono/química , Marcadores de Spin , Troponina C/química , Animais , Galinhas , Espectroscopia de Ressonância de Spin Eletrônica , Modelos Moleculares , Estrutura Terciária de Proteína , Estereoisomerismo , Troponina C/síntese químicaRESUMO
A novel ZMP analog 3 was synthesized from inosine in 10 steps, and exhibited small but significant inhibitory activity toward protein kinase C.
Assuntos
Aminoimidazol Carboxamida/análogos & derivados , Metano/análogos & derivados , Ribonucleotídeos/química , Aminoimidazol Carboxamida/síntese química , Aminoimidazol Carboxamida/química , Hidrocarbonetos , Espectroscopia de Ressonância Magnética , Metano/química , Estrutura Molecular , Ribonucleotídeos/síntese químicaRESUMO
The stereochemical course of the reaction catalyzed by a copper amine oxidase from Arthrobacter globiformis has been investigated using 2-phenylethylamine stereospecifically deuterium-labeled at the C1 position. Measurements of deuterium content in the product, phenylacetaldehyde, by gas chromatography-mass spectrometry revealed stereospecific abstraction of the pro-S hydrogen during the enzymatic oxidation, as predicted from the structure modeling for the enzyme-bound substrate.