RESUMO
Anatomic visualization and molecular typing of metastatic regional lymph nodes in breast cancer patients are a serious clinical challenge in modern oncology. According to the results of previous studies, [99mTc]Tc-(HE)3-G3 has proven to be a promising diagnostic agent in differentiating the HER2/neu receptor status in primary breast tumors (p < 0.05, Mann-Whitney test). In this regard, the purpose of this study is to explore the possibilities of using [99mTc]Tc-(HE)3-G3 to determine the HER2/neu receptor status in the metastatic axillary lymph nodes (mALNs) of breast cancer patients. The study was conducted using clinical material from 20 breast cancer patients (T2-4N1-3M0-1) before systemic therapy (10 patients with positive and 10 patients with negative HER2/neu expression in mALNs) who underwent SPECT/CT scan 4 h after the administration of [99mTc]Tc-(HE)3-G3. Morphological and immunohistochemical studies of mALNs with assessment of the HER2/neu status were performed on all patients. We found that mALN-to-background and mALN-to-latissimus dorsi muscle ratios for [99mTc]Tc-(HE)3-G3 uptake 4 h after its administration may be used for typing of the HER2/neu status in mALNs of breast cancer patients (p < 0.05, Mann-Whitney test). In that case, sensitivity and specificity for the mALN-to-background ratio were identical at 80%, with the threshold value being > 12.25.
RESUMO
The aim of our research was to study the apoptotic index and the representativeness of CD95-receptor in the effector cells, in addition to estimation of the content of tumor necrosis factor-alpha in the induced sputum supernatant observed in patients with bronchial asthma and chronic obstructive bronchitis. These indices were evaluated in the disease dynamics and involves various treatment programs. The object of our research was induced sputum. The analysis has proven that apoptotic index of effector cell nuclei, representativeness of CD95-receptor and the content of tumor necrosis factor-alpha in the induced sputum differed, respectively, in patients with bronchial asthma and chronical obstructive bronchitis, and depended on the period of disease and employed treatment. The induced sputum analysis results can be used for the airway inflammation activity monitoring, with regard to these diseases and to treatment efficiency monitoring.
Assuntos
Apoptose/imunologia , Asma/patologia , Bronquite Crônica/patologia , Escarro/citologia , Adulto , Asma/tratamento farmacológico , Asma/imunologia , Bronquite Crônica/tratamento farmacológico , Bronquite Crônica/imunologia , Técnica Indireta de Fluorescência para Anticorpo , Glucocorticoides/uso terapêutico , Humanos , Microscopia de Fluorescência , Pessoa de Meia-Idade , Escarro/imunologia , Fator de Necrose Tumoral alfa/imunologia , Receptor fas/imunologiaRESUMO
AIM: To examine the content of CD16, CD25, CD95, and HLA-DR antigenic determinants in induced sputum (IS) of patients with bronchial asthma (BA) and chronic obstructive bronchitis (COB). MATERIALS AND METHODS: The study enrolled 36 patients with BA and 15 with COB whose age was 21 to 58 years. The cellular precipitate of IS served as a material for the study. The studied receptors were determined by immunofluorescence using the "Clonospectrum" monoclonal antibodies. RESULTS: There was an increase in the content of CD16, CD25, HLA-DR, CD95 whose expression was decreased when glucocorticosteroids were used. At remission of asthma, the level of these receptors approximated that in the control group. On an exacerbation of COB, the content of these markers was significantly less than that in the controls and the use of antibacterial agents and methylxanthines led to its rise. At remission of COB, CD16 and CD25 cells were not recorded. CONCLUSION: The levels of membrane markers in IM of patients with BA and COB depend on the period of the disease and used therapy.