The heart of an acrobatic bird.

The courtship behavior of some species of birds can be energetically demanding, but it is unknown if cardiovascular specializations enable such behaviors. While performing a highly acrobatic courtship dance, heart rate in male golden-collared manakins increases briefly to 1300 beats per minute, among the highest heart rates recorded in any bird or mammal. We hypothesize that male manakins have enhanced cardiovascular capabilities to meet these demands on the heart. Using histological and molecular techniques, we examined manakin heart structure as well as expression of genes involved in Ca2+ handling, action potential duration, steroidal signaling and cardiac growth. These measures were also made on the hearts of zebra finches, a similar-sized bird with limited cardiovascular demands. Compared to the zebra finch, the manakin had a significantly thicker left ventricular (LV) muscle (cross-sectional thickness of the free LV wall and septum) with a smaller LV chamber. In addition, compared to zebra finches, manakin hearts had significantly greater gene expression of ryanodine receptors as well as androgen receptors. Testosterone (T) treatment of non-breeding manakins (with low T) increased gene expression of the Ca2+ pump SERCA. These observations suggest that hearts of breeding male manakins require specialized Ca2+ handling and androgens may facilitate manakin cardiovascular function.

Region-specific neural corticosterone patterns differ from plasma in a male songbird.

The adrenal hormone corticosterone (CORT) acts on brain to mediate physiology and behavior. In songbirds, behavioral effects of CORT vary across species, environmental conditions, and life history stage, with several mechanisms proposed to account for these divergent results. Although blood CORT levels are well characterized, few studies measure CORT within the brain itself. Here we used in vivo microdialysis to measure CORT in two regions of the zebra finch brain, the hippocampus (HP) and caudal nidopallium (cNp). Our results show that we can successfully measure physiological levels of CORT in brain within 15- to 30-minute intervals of dialysate collection. Moreover, we found that levels in the cNp were generally lower than levels in the HP. Surprisingly, whereas plasma CORT levels increased in response to a standard stressor, no stress-induced surge was detected in the HP or cNp. In addition, although a diel CORT rhythm was observed in plasma, the rhythm in brain was attenuated and only observed when levels were integrated over a 4-hour time period. Regional differences in brain CORT levels were reflected in local mRNA expression levels of the CORT-inactivating enzyme 11ß-hydroxysteroid dehydrogenase type 2 with levels elevated in the cNp relative to the HP. Region-specific CORT metabolism may therefore play a role in buffering the brain from CORT fluctuations.