Synthetic algocyanobacterial consortium as an alternative to chemical fertilizers.

The use of unregulated pesticides and chemical fertilizers can have detrimental effects on biodiversity and human health. This problem is exacerbated by the growing demand for agricultural products. To address these global challenges and promote food and biological security, a new form of agriculture is needed that aligns with the principles of sustainable development and the circular economy. This entails developing the biotechnology market and maximizing the use of renewable and eco-friendly resources, including organic fertilizers and biofertilizers. Phototrophic microorganisms capable of oxygenic photosynthesis and assimilation of molecular nitrogen play a crucial role in soil microbiota, interacting with diverse microflora. This suggests the potential for creating artificial consortia based on them. Microbial consortia offer advantages over individual organisms as they can perform complex functions and adapt to variable conditions, making them a frontier in synthetic biology. Multifunctional consortia overcome the limitations of monocultures and produce biological products with a wide range of enzymatic activities. Biofertilizers based on such consortia present a viable alternative to chemical fertilizers, addressing the issues associated with their usage. The described capabilities of phototrophic and heterotrophic microbial consortia enable effective and environmentally safe restoration and preservation of soil properties, fertility of disturbed lands, and promotion of plant growth. Hence, the utilization of algo-cyano-bacterial consortia biomass can serve as a sustainable and practical substitute for chemical fertilizers, pesticides, and growth promoters. Furthermore, employing these bio-based organisms is a significant stride towards enhancing agricultural productivity, which is an essential requirement to meet the escalating food demands of the growing global population. Utilizing domestic and livestock wastewater, as well as CO2 flue gases, for cultivating this consortium not only helps reduce agricultural waste but also enables the creation of a novel bioproduct within a closed production cycle.

Potential of cyanobacteria in the conversion of wastewater to biofuels.

Environmental and energy security has now become a serious global problem, requiring a lot of research to find and implement its cost-effective and environmentally friendly alternatives. The development and use of renewable energy sources is necessary and important in order to avoid the emergence of a global economic crisis. One of the solution to prevent a future crisis caused by energy shortages is to introduce biofuels into the fuel market. Despite the fact that various forms of renewable energy are currently used, the prospects for the production of biofuels from cyanobacteria are quite high due to their unique properties, such as a high lipid content and a suitable fatty acid (FA) composition for the production of biofuels, their suitability for growing open water and the ability to grow on wastewater. The purpose of this article is to provide a comprehensive overview of the potential of cyanobacteria in the conversion of wastewater into biofuels. The article covers comparative data on the accumulation of lipids and the content of fatty acids in various representatives of cyanobacteria and their possibilities in the remediation of wastewater. Various approaches to the extraction of lipids from phototrophic microorganisms that are currently available, their advantages and disadvantages, and the results of the monitoring of the main key points of the development of the technology for converting cyanobacterial biomass into biofuels, with an emphasis on the existing barriers, effects and solutions, are also considered. Further research in this field is required for the successful implementation of this technology on an industrial scale.

Reducing Heavy Metal Contamination in Soil and Water Using Phytoremediation.

The increase in industrialization has led to an exponential increase in heavy metal (HM) soil contamination, which poses a serious threat to public health and ecosystem stability. This review emphasizes the urgent need to develop innovative technologies for the environmental remediation of intensive anthropogenic pollution. Phytoremediation is a sustainable and cost-effective approach for the detoxification of contaminated soils using various plant species. This review discusses in detail the basic principles of phytoremediation and emphasizes its ecological advantages over other methods for cleaning contaminated areas and its technical viability. Much attention has been given to the selection of hyperaccumulator plants for phytoremediation that can grow on heavy metal-contaminated soils, and the biochemical mechanisms that allow these plants to isolate, detoxify, and accumulate heavy metals are discussed in detail. The novelty of our study lies in reviewing the mechanisms of plant-microorganism interactions that greatly enhance the efficiency of phytoremediation as well as in discussing genetic modifications that could revolutionize the cleanup of contaminated soils. Moreover, this manuscript discusses potential applications of phytoremediation beyond soil detoxification, including its role in bioenergy production and biodiversity restoration in degraded habitats. This review concludes by listing the serious problems that result from anthropogenic environmental pollution that future generations still need to overcome and suggests promising research directions in which the integration of nano- and biotechnology will play an important role in enhancing the effectiveness of phytoremediation. These contributions are critical for environmental scientists, policy makers, and practitioners seeking to utilize phytoremediation to maintain the ecological stability of the environment and its restoration.

Assessing the Efficacy of Cyanobacterial Strains as Oryza sativa Growth Biostimulants in Saline Environments.

Soil salinity, which affects plant photosynthesis mechanisms, significantly limits plant productivity. Soil microorganisms, including cyanobacteria, can synthesize various exometabolites that contribute to plant growth and development in several ways. These microorganisms can increase plant tolerance to salt stress by secreting various phytoprotectants; therefore, it is highly relevant to study soil microorganisms adapted to high salinity and investigate their potential to increase plant resistance to salt stress. This study evaluated the antioxidant activity of four cyanobacterial strains: Spirulina platensis Calu-532, Nostoc sp. J-14, Trichormus variabilis K-31, and Oscillatoria brevis SH-12. Among these, Nostoc sp. J-14 presented the highest antioxidant activity. Their growth-stimulating effects under saline conditions were also assessed under laboratory conditions. These results indicate that Nostoc sp. J-14 and T. variabilis K-31 show significant promise in mitigating the harmful effects of salinity on plant size and weight. Both strains notably enhanced the growth of Oryza sativa plants under saline conditions, suggesting their potential as biostimulants to improve crop productivity in saline environments. This research underscores the importance of understanding the mechanisms by which cyanobacteria increase plant tolerance to salt stress, paving the way for sustainable agricultural practices in saline areas.

Spectral insights: Navigating the frontiers of biomedical and microbiological exploration with Raman spectroscopy.

Raman spectroscopy (RS), a powerful analytical technique, has gained increasing recognition and utility in the fields of biomedical and biological research. Raman spectroscopic analyses find extensive application in the field of medicine and are employed for intricate research endeavors and diagnostic purposes. Consequently, it enjoys broad utilization within the realm of biological research, facilitating the identification of cellular classifications, metabolite profiling within the cellular milieu, and the assessment of pigment constituents within microalgae. This article also explores the multifaceted role of RS in these domains, highlighting its distinct advantages, acknowledging its limitations, and proposing strategies for enhancement.

Raman Spectroscopy and Its Modifications Applied to Biological and Medical Research.

Nowadays, there is an interest in biomedical and nanobiotechnological studies, such as studies on carotenoids as antioxidants and studies on molecular markers for cardiovascular, endocrine, and oncological diseases. Moreover, interest in industrial production of microalgal biomass for biofuels and bioproducts has stimulated studies on microalgal physiology and mechanisms of synthesis and accumulation of valuable biomolecules in algal cells. Biomolecules such as neutral lipids and carotenoids are being actively explored by the biotechnology community. Raman spectroscopy (RS) has become an important tool for researchers to understand biological processes at the cellular level in medicine and biotechnology. This review provides a brief analysis of existing studies on the application of RS for investigation of biological, medical, analytical, photosynthetic, and algal research, particularly to understand how the technique can be used for lipids, carotenoids, and cellular research. First, the review article shows the main applications of the modified Raman spectroscopy in medicine and biotechnology. Research works in the field of medicine and biotechnology are analysed in terms of showing the common connections of some studies as caretenoids and lipids. Second, this article summarises some of the recent advances in Raman microspectroscopy applications in areas related to microalgal detection. Strategies based on Raman spectroscopy provide potential for biochemical-composition analysis and imaging of living microalgal cells, in situ and in vivo. Finally, current approaches used in the papers presented show the advantages, perspectives, and other essential specifics of the method applied to plants and other species/objects.

Influence of Mo and Fe on Photosynthetic and Nitrogenase Activities of Nitrogen-Fixing Cyanobacteria under Nitrogen Starvation.

The potential of cyanobacteria to perform a variety of distinct roles vital for the biosphere, including nutrient cycling and environmental detoxification, drives interest in studying their biodiversity. Increasing soil erosion and the overuse of chemical fertilizers are global problems in developed countries. The option might be to switch to organic farming, which entails largely the use of biofertilisers. Cyanobacteria are prokaryotic, photosynthetic organisms with considerable potential, within agrobiotechnology, to produce biofertilisers. They contribute significantly to plant drought resistance and nitrogen enrichment in the soil. This study sought, isolated, and investigated nitrogen-fixing cyanobacterial strains in rice fields, and evaluated the effect of Mo and Fe on photosynthetic and nitrogenase activities under nitrogen starvation. Cyanobacterial isolates, isolated from rice paddies in Kazakhstan, were identified as Trichormus variabilis K-31 (MZ079356), Cylindrospermum badium J-8 (MZ079357), Nostoc sp. J-14 (MZ079360), Oscillatoria brevis SH-12 (MZ090011), and Tolypothrix tenuis J-1 (MZ079361). The study of the influence of various concentrations of Mo and Fe on photosynthetic and nitrogenase activities under conditions of nitrogen starvation revealed the optimal concentrations of metals that have a stimulating effect on the studied parameters.

Prospects for the creation of a waste-free technology for wastewater treatment and utilization of carbon dioxide based on cyanobacteria for biodiesel production.

Current fresh water and energy shortage determines the need to study the possibilities of using living objects in bioenergy and environmental purification technologies. The development of waste-free technologies allows waste recycling, which saves raw materials and energy, in turn, reducing waste generation. The effect of different carbon dioxide concentrations and wastewater from households on the growth of cyanobacteria was studied in order to determine their capabilities in the purification processes. It was found that the optimal CO2 concentration for the cultivation of cyanobacteria Cyanobacterium sp. IPPAS B-1200 and Desertifilum sp. IPPAS B-1220 was 10 %, and for the Cyanobacterium aponinum IPPAS B-1201 - 5%. It was revealed that the cultivation of the cyanobacterium Cyanobacterium sp. IPPASB-1200 on wastewater from the water storage reduces the concentration of organic pollutants and, accordingly, improves the physicochemical properties of water. The cleaning percentage for selected pollutants was 68-100 %. It was shown that the most optimal ratio of wastewater to nutrient media for cyanobacteria cultivation were 25:75 and 50:50. The lipid content (%/dry weight) in the biomass of the studied strains of cyanobacteria ranges from 15 to 22% after cultivation in wastewater. It was determined that the strains of Cyanobacterium genus were the most suitable for the production of biodiesel according to their fatty acids composition. It was determined that lipids were composed of only saturated and monounsaturated fatty acids. As a result of the studies, the optimal conditions for the growth of Cyanobacterium sp. IPPAS B-1200 were determined. This microorganism has a good potential to produce biodiesel as a producer of saturated and monounsaturated middle-chain-length fatty acids.