Degradation of bio-based film plastics in soil under natural conditions.

The degradation of bio-based plastic materials in field soil under natural conditions was investigated in this study. Three bio-based plastics materials, which contained polylactide (PLA) with polybutylene adipate terephthalate and additives (PLA_1), PLA-based polyester blend with mineral filler (PLA_2), and polybutylene succinate with mineral filler (PBS_1) in the form of the film, were subjected to soil burial biodegradation processes. The experiments were carried out in a climate with an average annual temperature of 9.4 °C, in winter and summer periods for one year. The degradation of the materials was evaluated by macro- and microscopic observations, weight loss, thermogravimetric analysis, and tensile test. Macroscopic observation indicated that changes in the color of film surface were visible for samples PBS_1 after 12 months of degradation. Using microscopic inspection the erosion of surface samples PLA_1 and PBS_1 after 12 months was observed. Mass loss of samples PLA_1 and PLA_2 after one year of degradation were below 0.6 %. Moreover, for PBS_1 sample, mass loss was equal to 4.3 %. Based on the obtained results of the mass loss, a description of the degradation kinetics was proposed, showing the changes in the thickness of the tested polymer over time. The thermal stability of the samples PLA_1 and PLA_2 decreased during the degradation process by 16.1 and 2.6 °C, respectively, and for PBS_1 increased by 1.7 °C. Tensile strength at break after 12 months of degradation decreased for sample PLA_1 and PLA_2 by 27.3 and 5.8 %, respectively, and increased for sample PBS_1 by 28.2 % compare to unexposed sample.

Influence of initial pH on the production of volatile fatty acids and hydrogen during dark fermentation of kitchen waste.

The purpose of this work was to determine the effect of initial pH on the production of volatile fatty acids (VFA) and hydrogen (H2) in the dark fermentation processes of kitchen waste. The study was conducted in batch bioreactors of working volume 1 L for different initial pH in the range from 5.5 to 9.0. The dark fermentation processes were carried out for 4 days at 37°C. Initial organic load of the kitchen waste in all bioreactors amounted to 25.5 gVS/L. Buffering of pH during the fermentation process was carried out with the use of ammonia contained mainly in digested sludge. The optimal conditions for the production of VFA and H2 were achieved at the initial pH of 8. Production of VFA and H2 in these conditions was, respectively, 13.9 g/L and 72.4 mL/gVS. The main produced components of VFA were acetic and butyric acids. The production of ethanol and lactic acid was at very low levels due to the high ratio of the volatile fatty acids to total organic content of 0.86. With the optimal initial pH of 8 the yield of CO2 production was 0.30 gC/gC. High initial pH value (above 8) extended the lag phase duration in the course of H2 production. The dominant groups of micro-organisms at the most favourable initial pH of 8 for the production of VFA and H2 were Bacteroidetes, Firmicutes, Spirochaetes and Waste Water of Evry 1 (WWE1) at the phylum level.

Production of volatile fatty acids and H2 for different ratio of inoculum to kitchen waste.

The aim of this study was to evaluate the effect of different inoculum ratio on the dark fermentation of kitchen waste in terms of volatile fatty acids (VFAs) and H2 production. The experiments were performed in batch bioreactors of effective volume 1 L without pH regulation. The ratio between the DS and KW was being increased from 0.11 to 0.51 on a volatile solids (VS) basis, while the initial content of KW was equal to 34.1 g VS/L. Increase of the DS/KW ratio from 0.11 to 0.28 resulted in the rise of VFAs and H2 production. Further increase in the amount of added DS did not cause a significant change in the production of VFAs and H2. In the bioreactor with the DS/KW ratio of 0.28, the production of VFAs and H2 was equal to 16.0 g/L and 68.1 mL/g VS, respectively. Acetic and butyric acids were produced in the largest amount and their content, for DS/KW ratio of 0.28, were equal 37% and 43%, respectively. At the ratio of DS/KW above 0.4, the caproic acid content attained the level of 25%. Based on the DS and KW microbiological analysis, it was observed that dominant bacteria were Bacteroidetes, Firmicutes, Proteobacteria, Spirochaetes and WWE1 at the phylum level.

The effect of initial organic load of the kitchen waste on the production of VFA and H2 in dark fermentation.

Dark fermentation of kitchen wastes was studied in batch bioreactors, with no pH adjustment, to evaluate the effect of the initial organic load on the process performance in terms of volatile fatty acids and H2 production. Initial organic load of the kitchen wastes ranged from 4.1 to 48.2gVS/L. Acetic and butyric acids were produced in the largest amount. At the initial organic load of 48.2KWgVS/L the highest concentration of volatile fatty acids was 9.81g/L. The maximum production yield of H2 (76.1mL/gVS) was found for the initial organic load of kitchen wastes at 14.3gVS/L. The carbon balance calculation showed that the maximum CO2 yield of 0.34 gC/gC was attained in the bioreactor with the initial organic load of 14.3gVS/L. The microbiological analysis revealed that the predominant microorganisms in the dark fermentation process were Bacteroidetes, Firmicutes, Spirochaetes and WWE1 at phyla level.

Degradation of municipal solid waste in simulated landfill bioreactors under aerobic conditions.

In this study the municipal solid waste degradation processes in simulated landfill bioreactors under aerobic and anaerobic conditions is investigated. The effect of waste aeration on the dynamics of the aerobic degradation processes in lysimeters as well as during anaerobic processes after completion of aeration is presented. The results are compared with the anaerobic degradation process to determine the stabilization stage of waste in both experimental modes. The experiments in aerobic lysimeters were carried out at small aeration rate (4.41⋅10(-3)lmin(-1)kg(-1)) and for two recirculation rates (24.9 and 1.58lm(-3)d(-1)). The change of leachate and formed gases composition showed that the application of even a small aeration rate favored the degradation of organic matter. The amount of CO2 and CH4 released from anaerobic lysimeter was about 5 times lower than that from the aerobic lysimeters. Better stabilization of the waste was obtained in the aerobic lysimeter with small recirculation, from which the amount of CO2 produced was larger by about 19% in comparison with that from the aerobic lysimeter with large leachate recirculation.

Influence of pretreatment with Fenton's reagent on biogas production and methane yield from lignocellulosic biomass.

Biomass from Miscanthus giganteus, Sida hermaphrodita and Sorghum Moensch was treated with Fenton's reagent for 2 hours under optimal conditions (pH=3, mass ratio of [Fe(2+)]:[H(2)O(2)] equals 1:25 for Miscanthus and Sorghum and 1:15 for Sida). The degrees of delignification were 30.3%, 62.3% and 48.1% for the three plant species, respectively. The volatile fatty acids concentration after chemical pretreatment was high enough for production of biogas with a high methane content. Combined chemical oxidation and enzymatic hydrolysis with cellulase and cellobiase led to glucose contents of above 4 g/L. Among the tested plants, the highest biogas production (25.2 Ndm(3)/kg TS fed) with a 75% methane content was obtained with Sorghum Moensch. The results of the three-step process of biomass degradation show the necessity of applying a chemical pretreatment such as oxidation with Fenton's reagent. Moreover, the coagulation of residual Fe(3+) ions is not required for high biogas production.