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1.
Int Microbiol ; 2023 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-37971657

RESUMO

The increase in simultaneous exposure to magnetic fields and other hazardous compounds released from industrial applications poses multiple stress conditions on the ecosystems and public human health. In this work, we investigated the effects of co-exposure to a static magnetic field (SMF) and silver ions (AgNO3) on biochemical parameters and antioxidant enzyme activities in the yeast Saccharomyces cerevisiae. Sub-chronic exposure to AgNO3 (0.5 mM) for 9 h resulted in a significant decrease in antioxidant enzyme activity, including glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and glutathione transferase (GST). The total glutathione (GSH) level increased in yeast cells exposed to Ag. Additionally, a notable elevation in malondialdehyde (MDA) levels and protein carbonyl content was observed in both the AgNP and AgNO3 groups compared to the control group. Interestingly, the SMF alleviated the oxidative stress induced by silver nitrate, normalizing antioxidant enzyme activities by reducing cellular ROS formation, MDA levels, and protein carbonylation (PCO) concentrations.

2.
Sci Rep ; 11(1): 20078, 2021 10 11.
Artigo em Inglês | MEDLINE | ID: mdl-34635721

RESUMO

The bacteriocidal properties of silver nanoparticles (AgNPs) depend on their average diameter (toxicity increases with decreasing diameter). In the present work, we describe novel green chemistry biosynthesis of AgNPs from AgNO3 added to cell-free culture medium of baker's yeast, Saccharomyces cerevisiae, yielding nanoparticles in the range 11-25 nm. However, when yeast was grown in a moderate static magnetic field, AgNPs obtained from the resulting cell-free culture medium, were significantly smaller (2-12 nm) than those obtained without magnetic field. These latter nanoparticles were highly crystalline, stable and near-uniform shape. Furthermore, the antibacterial activity of AgNPs obtained from static magnetic fields were greater than those from control cultures. Static magnetic fields show a promising ability to generate biocidal nanoparticles via this novel green chemistry approach.


Assuntos
Antibacterianos/administração & dosagem , Bactérias/efeitos dos fármacos , Química Verde/métodos , Nanopartículas Metálicas/administração & dosagem , Saccharomyces cerevisiae/química , Prata/química , Antibacterianos/química , Sistema Livre de Células , Nanopartículas Metálicas/química
3.
PLoS One ; 14(1): e0209843, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30608963

RESUMO

Exposure to static magnetic fields (SMF) can cause changes in microorganism metabolism altering key subcellular functions. The purpose of this study was to investigate whether an applied SMF could induce biological effects on growth of Saccharomyces cerevisiae, and then to probe biochemical and bio-molecular responses. We found a decrease in growth and viability under SMF (250mT) after 6h with a significant decrease in colony forming units followed by an increase between 6 h and 9 h. Moreover, measurements of antioxidant enzyme activities (catalase, superoxide dismutase, glutathione peroxidase) demonstrated a particular profile suggesting oxidative stress. For instance, SOD and catalase activities increased in magnetized cultures after 9 h compared with unexposed samples. However, SMF exposure caused a decrease in glutathione peroxidase activity. Finally, SMF caused an increase in MDA levels as well as the content of protein carbonyl groups after 6 and 9 h of exposure.


Assuntos
Campos Magnéticos/efeitos adversos , Estresse Oxidativo/fisiologia , Saccharomyces cerevisiae/metabolismo , Antioxidantes/metabolismo , Catalase/metabolismo , Glutationa Peroxidase/metabolismo , Malondialdeído/metabolismo , Oxirredução , Saccharomyces cerevisiae/crescimento & desenvolvimento , Superóxido Dismutase/metabolismo
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