Non-surgical management and obstetric outcomes of heterotopic interstitial pregnancies.

Objective: To assess the effectiveness and safety of non-surgical management for six heterotopic interstitial pregnancies.Material and methods: We retrospectively analyzed the data of six women diagnosed with heterotopic interstitial pregnancies who underwent non-surgical treatment at the CHA Bundang Medical Center between January 2007 and December 2017. Three heterotopic interstitial pregnancies were treated with sono-guided potassium chloride (KCl) injections. Two cases were managed expectantly. One heterotopic quadruplet pregnancy with twin, left interstitial, and tubal pregnancy was treated by sono-guided KCl injection and laparoscopic left salpingectomy. Complications and outcomes were measured.Results: Three cases were treated with sono-guided KCl injection and the intrauterine pregnancy continued to term. Intrauterine pregnancies were vaginally delivered without complications. One case that was treated expectantly was delivered at full term, while the other case resulted in spontaneous abortion. Quadruplet heterotopic pregnancy was successfully managed with sono-guided KCl injection and laparoscopic salpingectomy. Intrauterine twin pregnancy was successfully delivered by elective cesarean section at 37 + 0 weeks of gestation with healthy babies. Conclusions: KCl injection under ultrasonographic guidance could be a safer and more effective treatment option than surgical treatment in hemodynamically stable patients with fetal cardiac activity in interstitial pregnancy. Expectant management could be an option for patients with no fetal cardiac activity.

Maternal predictors of children's mental health in low-income families: A structural equation model.

Low-income populations are particularly susceptible to mental health problems, and the susceptibilities of family members may be interconnected. In particular, maternal factors are known to be linked to their children's outcomes. This study aims to investigate how maternal cognition, depression, and the mother-child relationship, as well as children's cognition, predict the mental health of children in low-income families. Pairs of mothers and children from families receiving governmental assistance were surveyed between January 2018 and March 2019. Korean versions of the following instruments were used: Strengths and Difficulties Questionnaire (children's mental health problems), Cognitive Triad Inventory for Children (children's cognition), Kerns' Security Scale (mother-child relationship), Center for Epidemiologic Studies Depression Scale (maternal depression), and Automatic Thoughts Questionnaire-Negative (maternal cognition). A structural equation model was used to examine how maternal cognition, depression, the mother-child relationship, and children's cognition predict children's mental health. Maternal negative cognition and depression mediated by the children's relationships with their mothers negatively predicted their cognition and mental health problems. Enhancing maternal mental health and a mother-child relationship can help improve positive cognition and mental health of children from low-income families.

Complete Genome Sequences of Two Apple Stem Grooving Viruses in Cnidium officinale in Korea.

We determined the whole-genome sequences of two apple stem grooving viruses (ASGV) detected in infected Cnidium officinale plants. The analyzed ASGV genomes were 6,494 nucleotides long and encoded two overlapping open reading frames. Phylogenetic analysis revealed the two ASGV isolates to be most closely related to the ASGV isolate Xinjiang-3.

Association between chronotype, tobacco use or alcohol use, and high-risk drinking by age group: The Seventh Korea National Health and Nutrition Examination Survey (VII: 2016-2017).

OBJECTIVES: To analyze the association between chronotype, tobacco use or alcohol use, and high-risk drinking by age group and examine the differences across groups. METHODS: A cross-sectional descriptive study design was adopted. Data from 12,189 participants aged ≥ 12 years from 2016 to 2017, excluding shift workers, were gathered from the Korea Disease Control and Prevention Agency's Seventh Korea National Health and Nutrition Examination Survey. A general linear model was used to determine the aforementioned associations and differences across age groups. Chronotype was measured using the Munich Chronotype Questionnaire. It was divided into quintiles. We used current tobacco use, monthly alcohol use, and high-risk drinking as indexes of tobacco use and alcohol use. These data were extracted from the national representative data. RESULTS: Among adolescents and adults, both tobacco use and alcohol use showed a linear increasing trend, although it was greatest among adolescents. In older adults, tobacco use showed a linear increasing trend with delayed chronotype. When the chronotypes of all age groups were aggregated, high-risk drinking was higher in both extreme types, Quintiles 1 and 5. CONCLUSIONS: Tobacco use and alcohol use were mostly associated with delayed chronotype. Therefore, interventions to address delayed chronotype at all ages are needed. As the trend of increase in tobacco use and alcohol use in adolescents was higher than in other age groups, a special strategy is required aimed at this population. Moreover, both extreme chronotypes should be considered risk factors for high-risk drinking.

Characterization of Extended-Spectrum Cephalosporin (ESC) Resistance in Salmonella Isolated from Chicken and Identification of High Frequency Transfer of blaCMY-2 Gene Harboring Plasmid In Vitro and In Vivo.

A total of 136 Salmonella isolates from chicken feces and meat samples of the top 12 integrated chicken production companies throughout Korea were collected. Among the 17 ESC-resistant Salmonella; blaCTX-M-15 was the most prevalent gene and two strains carried blaTEM-1/blaCTX-M-15 and blaCMY-2, respectively. The transferable blaCTX-M-15 gene was carried by IncFII plasmid in three isolates and the blaCMY-2 gene carried by IncI1 plasmid in one isolate. blaCMY-2 gene-harboring strain was selected as the donor based on the high frequency of blaCMY-2 gene transfer in vitro and its transfer frequencies were determined at 10-3 transconjugants per recipient. The transfer of blaCMY-2 gene-harboring plasmid derived from chicken isolate into a human pathogen; enteroinvasive Escherichia coli (EIEC), presented in mouse intestine with about 10-1 transfer frequency without selective pressure. From the competition experiment; blaCMY-2 gene-harboring transconjugant showed variable fitness burden depends on the parent strains. Our study demonstrated direct evidence that the blaCMY-2 gene harboring Salmonella from chicken could frequently transfer its ESC-resistant gene to E. coli in a mouse intestine without antimicrobial pressure; resulting in the emergence of multidrug resistance in potentially virulent EIEC isolates of significance to human health; which can increase the risk of therapeutic inadequacy or failures.

Longitudinal Study of the Distribution of Antimicrobial-Resistant Campylobacter Isolates from an Integrated Broiler Chicken Operation.

The aim of this study was to analyze the prevalence, antimicrobial resistance, and genetic diversity of Campylobacter isolates that were obtained from whole chicken production stages in Korea. A total of 1348 samples were collected from 10 production lines. The prevalence of Campylobacter in breeder farm, broiler farm, slaughterhouse, and retail meat products was 50.0%, 3.3%, 13.4%, and 68.4%, respectively, and Campylobacter was not detected at the hatchery stage. Resistance to quinolones/fluoroquinolones was the most prevalent at all stages. Among the multidrug-resistant isolates, 16 isolates (19.8%) from breeder farm were resistant to both azithromycin and ciprofloxacin. A total of 182 isolates were subdivided into 82 pulsed-field gel electrophoresis (PFGE) genotypes with 100% similarity. Diverse genotypes were presented with discontinuous patterns along the whole production chain. Thirty percent of Campylobacter-free flocks became positive after slaughtering. An identical genotype was simultaneously detected from both breeder farm and retail meat, even from different production lines. This study reveals that antimicrobial-resistant Campylobacter contamination can occur at all stages of the chicken supply chain. In particular, the breeder farm and slaughterhouse should be the main control points, as they are the potential stages at which antimicrobial-resistant Campylobacter could spread to retail meat products by horizontal transmission.

The Detection of Plant Viruses in Korean Ginseng (Panax ginseng) through RNA Sequencing.

Korean ginseng (Panax ginseng) is a dicotyledonous, medicinal, perennial plant belonging to the genus Panax of the family Araliaceae. We investigated the occurrence and incidence of plant viruses in Panax ginseng in Korea. A total of 656 leaf samples were combined into one and total RNA was extracted from the polled sample, using RNA sequencing (RNA-Seq), a metatranscriptome analysis of the plant virome was conducted. The virus present in Panax ginseng was confirmed by reverse transcription polymerase chain reaction (RT-PCR) assay using virus-specific primers. In RNA-Seq data analysis, the multiplication protein of four viral contigs including Aristotelia chilensis virus 1 (AcV1), Turnip mosaic virus (TuMV), Watermelon mosaic virus (WMV), and Tobamovirus multiplication protein were discovered. From our metatranscriptome analysis and RT-PCR assay, TuMV and WMV were detected, whereas the three viruses reported in China such as tomato yellow leaf curl China virus; panax notoginseng virus A; and panax virus Y were not found in this study. The distribution of domestic ginseng viruses seems different from that recorded in China. Overall, this is the first plant virome analysis of Panax ginseng in Korea.