RESUMO
The successful bone marrow transplantation presents the highest demands on the compatibility between donor and recipient in the antigen products of HLA system, namely in transplantations performed in unrelated individuals. Traditionally used serotyping gives rather exact results in class I HLA antigen determination, but when typing of class II antigens, at least 25% errors occur. Our papers presents a comparison of different molecular genetics methods used in class II HLA antigen typing. First results of class II HLA typing using RFLP technique, as performed in the Institute of Hematology and Blood Transfusion, are summarized.
Assuntos
Alelos , Antígenos HLA-D/genética , Teste de Histocompatibilidade , Impressões Digitais de DNA , Teste de Histocompatibilidade/métodos , Humanos , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de RestriçãoRESUMO
New findings on the pseudoautosomal area of X and Y chromosomes potentiate the importance of this part of the human genome for direct and indirect DNA diagnosis. The submitted paper is focused on the characteristic features of the given area with special emphasis on loci used in the Institute of Haematology and Blood Transfusion during DNA monitoring of patients after allogeneic transplantation of bone marrow and which thus provide important information with a major clinical impact. The authors indicate trends of future research focused on investigation of the polymorphism of the receptor of the haematopoietic factor GM CSF in relation to its function and the influence on the pathogenesis of AML.