Remote temperature sensing by the compact thermal imager from the International Space Station.

A systematic calibration approach is presented to correlate the digital output of an infrared camera and the scene temperature. Aided by the optoelectronic properties of the camera, as few as two experimental data points are needed to establish this correlation. This approach can readily include the effects of atmospheric transmission, scene emissivity, and different background subtractions. Hence, the temperature conversion in flight can be reliably obtained from laboratory calibration. The conversion function can also be used to identify the camera's thermal sensitivity and temperature resolution, which are important information in different space missions. In applying this calibration procedure to a laboratory camera and the compact thermal imager onboard the International Space Station, its validity is confirmed.

Mental health: who is more vulnerable to high work intensity? Evidence from Australian longitudinal data.

AIM: The adverse impacts of exposure to work intensity on mental health have been widely studied. However, there is a lack of research examining who is most vulnerable in terms of position on the mental health distribution. The current study aims to: (a) initially estimate the average impacts of work intensity on workers' mental health in Australia, and then (b) estimate the extent to which this effect varies across the mental health distribution. MATERIALS AND METHODS: The current study uses data from waves 2005--2017 of the Household Income and Labour Dynamics in Australia (HILDA) survey. It first employs Average Treatment Effect (ATE) to provide a baseline/average treatment effect for the whole population, and then applies Quantile Regression fixed effects models for various quantiles on the mental health distribution. DISCUSSION AND CONCLUSION: The baseline estimates show that there are significantly negative effects of work intensity on mental health for the whole population, but importantly the quantile fixed effect estimates show that these adverse effects are substantially stronger for those with the poorest mental health (i.e. at the bottom of the distribution). When ATE alone is estimated, the significant effect is averaged over the mental health distribution, missing important information regarding the heterogeneity of the effect. The findings have important implications for understanding and reducing mental health inequality, particularly inequality driven by workplace stress. First, they align with existing research demonstrating the importance of reducing psychosocial job stressors. Second, given workers with mental health problems were most susceptible to the adverse effects of work intensity, there is a need to offer additional support (and be sensitive of workloads) for this group in particular.

Strained-layer-superlattice-based compact thermal imager for the International Space Station.

The compact thermal imager (CTI) is a dual-band, strained-layer-superlattice (SLS) detector-based instrument that was installed on the exterior of the International Space Station (ISS) in conjunction with the third Robotic Refueling Mission 3 (RRM3) in 2018. The CTI serves as a pathfinder for future thermal infrared capability on Landsat. The CTI incorporates an SLS hybrid, a dual-band 3-5 and 8-10 µm, electrically switchable, 320×256 array with 30 µm2 pixels, bonded to an Indigo ISC0903 Readout Integrated Circuit (ROIC). The telescope was built around an integrated detector cryocooler assembly developed under a NASA Small Business Innovative Research award with QmagiQ, LLC. The cooler is a Ricor K508 and the front-end optics is a custom-designed, doublet lens telescope with a 150 mm focal length. The ground resolution is 80 meters/pixel from the ISS altitude of 400 km. A filter creates two spectral channels from the dual bands, 3.3-5.4 and 7.8-10.2 µm. The detector hybrid control electronics is a custom-developed system based on the Teledyne Imaging Systems SIDECAR Application-Specific Integrated Circuit. This module provides the electronic interface from the RRM3 SpaceCube on-board processor to the detector/ROIC assembly. The primary goal of this mission was to perform a technology demonstration of the SLS technology and the commercial cooler technology elevating the Technology Readiness Level (TRL) to TRL 9 on a bare-bones budget and relatively fast development cycle. Some science objectives include locating fires, approximating land surface temperatures, and monitoring evapotranspiration, sea ice, and glacier dynamics. In this paper, we will present the design of the focal plane, optics, electronics, and mechanical structure of the CTI. We will also describe the operation and qualification tests that were performed to bring the CTI to the NASA TRL 6 in preparation for the launch on a SpaceX Dragon from the Kennedy Space Center.

Shear Conditioning of Adipose Stem Cells for Reduced Platelet Binding to Engineered Vascular Grafts.

Conferring antithrombogenicity to tissue-engineered vascular grafts remains a major challenge, especially for urgent bypass grafting that excludes approaches based on expanding autologous endothelial cells (ECs) that requires weeks of cell culture. Adipose-derived stem cells (ASCs) are available from most patients in sufficient number for coronary bypass graft seeding and may be effective as allogeneic cells. We thus compared the adhesion and platelet binding of human ASCs that were shear conditioned with constant and pulsatile shear stress (SS) after seeding the cells on a biologically engineered matrix suitable for arterial grafts. A monolayer of cells was maintained up to 15 dyn/cm2 constant SS and up to 15 dyn/cm2 mean pulsatile SS for 6 days of shear flow. Platelet binding was reduced from 83% to 6% of surface area and nitric oxide production was increased 23-fold with 7.5-15 dyn/cm2 constant SS, but not pulsatile SS, relative to cells cultured statically on the matrix for 6 days. The reduction in platelet binding varied from no reduction to maximum reduction over a constant shear range of ∼2 to 4 dyn/cm2, respectively. Collectively, the study supports the potential use of ASCs to seed the luminal surface of a vascular graft made from this biologically engineered matrix to confer an antithrombogenic surface during the development of an endothelium from the seeded cells or the surrounding blood and tissue.

Optical imaging predicts mechanical properties during decellularization of cardiac tissue.

Decellularization of xenogeneic hearts offers an acellular, naturally occurring, 3D scaffold that may aid in the development of an engineered human heart tissue. However, decellularization impacts the structural and mechanical properties of the extracellular matrix (ECM), which can strongly influence a cell response during recellularization. We hypothesized that multiphoton microscopy (MPM), combined with image correlation spectroscopy (ICS), could be used to characterize the structural and mechanical properties of the decellularized cardiac matrix in a noninvasive and nondestructive fashion. Whole porcine hearts were decellularized for 7 days by four different solutions of Trypsin and/or Triton. The compressive modulus of the cardiac ECM decreased to < 20% of that of the native tissue in three of the four conditions (range 2-8 kPa); the modulus increased by -150% (range 125-150 kPa) in tissues treated with Triton only. The collagen and elastin content decreased steadily over time for all four decellularization conditions. The ICS amplitude of second harmonic generation (SHG, ASHG) collagen images increased in three of the four decellularization conditions characterized by a decrease in fiber density; the ICS amplitude was approximately constant in tissues treated with Triton only. The ICS ratio (R(SHG), skew) of collagen images increased significantly in the two conditions characterized by a loss of collagen crimping or undulations. The ICS ratio of two-photon fluorescence (TPF, R(TPF)) elastin images decreased in three of the four conditions, but increased significantly in Triton-only treated tissue characterized by retention of densely packed elastin fibers. There were strong linear relationships between both the log of A(SHG) (R(2) = 0.86) and R(TPF) (R(2) = 0.92) with the compressive modulus. Using these variables, a linear model predicts the compressive modulus: E=73.9 × Log(A(SHG))+70.1 × R(TPF) - 131 (R(2) = 0.94). This suggests that the collagen content and elastin alignment determine the mechanical properties of the ECM. We conclude that MPM and ICS analysis is a noninvasive, nondestructive method to predict the mechanical properties of the decellularized cardiac ECM.