Exposure to diesel exhaust alters the functional metagenomic composition of the airway microbiome in former smokers.

The lung microbiome plays a crucial role in airway homeostasis, yet we know little about the effects of exposures such as air pollution therein. We conducted a controlled human exposure study to assess the impact of diesel exhaust (DE) on the human airway microbiome. Twenty-four participants (former smokers with mild to moderate COPD (N = 9), healthy former smokers (N = 7), and control healthy never smokers (N = 8)) were exposed to DE (300 µg/m3 PM2.5) and filtered air (FA) for 2 h in a randomized order, separated by a 4-week washout. Endobronchial brushing samples were collected 24 h post-exposure and sequenced for the 16S microbiome, which was analyzed using QIIME2 and PICRUSt2 to examine diversity and metabolic functions, respectively. DE exposure altered airway microbiome metabolic functions in spite of statistically stable microbiome diversity. Affected functions included increases in: superpathway of purine deoxyribonucleosides degradation (pathway differential abundance 743.9, CI 95% 201.2 to 1286.6), thiazole biosynthesis I (668.5, CI 95% 139.9 to 1197.06), and L-lysine biosynthesis II (666.5, CI 95% 73.3 to 1257.7). There was an exposure-by-age effect, such that menaquinone biosynthesis superpathways were the most enriched function in the microbiome of participants aged >60, irrespective of smoking or health status. Moreover, exposure-by-phenotype analysis showed metabolic alterations in former smokers after DE exposure. These observations suggest that DE exposure induced substantial changes in the metabolic functions of the airway microbiome despite the absence of diversity changes.

Effect of a rapid maxillary expansion on snoring and sleep in children: a pilot study.

AIM: The aim of this study was to assess the efficacy of the McNamara rapid palatal expansion device for the treatment of sleep disorders in children. METHODS: The sample enrolled 12 children aged 4-11 years. Children with snoring and bruxism whose parents did not agree to tonsil surgery were included in the study. During the initial evaluation, a questionnaire addressing sleep was administered, and plaster models were made for the construction of the McNamara rapid maxillary expansion device. The expansion period was 7-15 days, and the McNamara device was removed after 6-8 months. The same questionnaire was administered again after 30 days of use of the orthopedic appliance. The data were analyzed using the McNemar test, with the level of significance set to 5% (P<0.05). RESULTS: Significant improvements were found in tiredness upon waking (P=0.002), mood (P=0.008), lip seal (P=0.031), drooling during sleep (P=0.031), snoring (P=0.001), and bruxism (P=0.0062). CONCLUSION: The use of non-invasive methods, such as rapid maxillary expansion, can be an effective treatment for snoring and other undesirable sleep behaviors in children.

Smoking among hospitalized patients in a general hospital.

OBJECTIVE: To determine the frequency of smoking among hospitalized patients in a general hospital, and to evaluate their profile. METHODS: A random representative sample of 111 patients, classified as nonsmokers, former smokers or smokers, was evaluated. The smokers were submitted to the Fagerström test and measurement of expired carbon monoxide. Expired carbon monoxide higher than 6 ppm was considered a significant indicator of recent smoking. RESULTS: Of the 111 patients in the sample, 60 (54%) were female. The mean age was 70 years. Of the 111 patients, 56 (51%) had never smoked, 36 (32%) were former smokers, and 19 (17%) were smokers. All of the smokers were male. The smokers were younger (58 +/- 17 years) than the nonsmokers (68 +/- 14 years) and the former smokers (73 +/- 14 years)--ANOVA: F = 6.57 (p = 0.002). Among the smokers, the mean tobacco intake was 43 pack-years and the mean Fagerström score was 5.0. Of the 19 smokers, 11 (58%) had respiratory symptoms and 3 had withdrawal symptoms. The mean expired carbon monoxide in the smokers was 5.0 ppm. Expired carbon monoxide levels were higher than 6 ppm in 8 (42%) of the smokers. There was a higher prevalence of smokers in some wards: 70% of all smokers were hospitalized on only five wards. CONCLUSIONS: In a large tertiary hospital, 17% of the hospitalized patients were smokers, and 7% had smoked within the last 8 hours. The smokers were younger men, hospitalized on specific wards.

Tabagismo em pacientes internados em um hospital geral / Smoking among hospitalized patients in a general hospital

OBJETIVO: Determinar a freqüência do tabagismo em pacientes internados em um hospital geral, e caracterizar o perfil dos fumantes hospitalizados. MÉTODOS: Foi avaliada uma amostra representativa e aleatória de 111 pacientes internados, classificados como não-fumantes, ex-fumantes e fumantes. Nos fumantes foi aplicado o questionário de Fagerström e obtidas medidas de monóxido de carbono no ar expirado. Valores acima de 6 ppm de monóxido de carbono no ar expirado foram considerados significantes para tabagismo recente. RESULTADOS: Dos 111 pacientes, 60 (54%) eram do sexo feminino. A média de idade foi de 70 anos. Do total, 56 pacientes (51%) nunca fumaram, 36 (32%) eram ex-fumantes e 19 (17%) eram fumantes atuais. Todos os fumantes eram do sexo masculino. Os fumantes tinham menor idade (58 ± 17 anos), em comparação aos não-fumantes (68 ± 14 anos) e ex-fumantes (73 ± 14 anos)-ANOVA: F = 6,57 (p = 0,002). A carga tabágica média dos fumantes foi de 43 anos-maço. A média do escore de Fagerström foi de 5,0. Dos 19 fumantes, 11 (58%) tinham sintomas respiratórios, e 3 tinham sintomas de abstinência. A média de monóxido de carbono no ar expirado dos fumantes foi 5,0 ppm. Oito (42%) dos fumantes tinham níveis de monóxido de carbono no ar expirado acima de 6 ppm. Houve maior prevalência de tabagistas em algumas clinicas: 70% dos fumantes se encontravam em apenas cinco enfermarias. CONCLUSÕES: Em um grande hospital terciário, 17% dos pacientes internados eram fumantes e destes 7% fumaram nas últimas 8 horas. Os fumantes eram pacientes mais jovens do sexo masculino, internados em determinadas clínicas.

OBJECTIVE: To determine the frequency of smoking among hospitalized patients in a general hospital, and to evaluate their profile. METHODS: A random representative sample of 111 patients, classified as nonsmokers, former smokers or smokers, was evaluated. The smokers were submitted to the Fagerström test and measurement of expired carbon monoxide. Expired carbon monoxide higher than 6 ppm was considered a significant indicator of recent smoking. RESULTS: Of the 111 patients in the sample, 60 (54%) were female. The mean age was 70 years. Of the 111 patients, 56 (51%) had never smoked, 36 (32%) were former smokers, and 19 (17%) were smokers. All of the smokers were male. The smokers were younger (58 ± 17 years) than the nonsmokers (68 ± 14 years) and the former smokers (73 ± 14 years)-ANOVA: F = 6.57 (p = 0.002). Among the smokers, the mean tobacco intake was 43 pack-years and the mean Fagerström score was 5.0. Of the 19smokers, 11 (58%) had respiratory symptoms and 3 had withdrawal symptoms. The mean expired carbon monoxide in the smokers was 5.0 ppm. Expired carbon monoxide levels were higher than 6 ppm in 8 (42%) of the smokers. There was a higher prevalence of smokers in some wards: 70% of all smokers were hospitalized on only five wards. CONCLUSIONS: In a large tertiary hospital, 17% of the hospitalized patients were smokers, and 7% had smoked within the last 8 hours. The smokers were younger men, hospitalized on specific wards.