Establishing a prognostic model of chromatin modulators and identifying potential drug candidates in renal clear cell patients.

BACKGROUND: Renal carcinoma is a common malignant tumor of the urinary system. Advanced renal carcinoma has a low 5-year survival rate and a poor prognosis. More and more studies have confirmed that chromatin regulators (CRs) can regulate the occurrence and development of cancer. This article investigates the functional and prognostic value of CRs in renal carcinoma patients. METHODS: mRNA expression and clinical information were obtained from The Cancer Genome Atlas database. Univariate Cox regression analysis and LASSO regression analysis were used to select prognostic chromatin-regulated genes and use them to construct a risk model for predicting the prognosis of renal cancer. Differences in prognosis between high-risk and low-risk groups were compared using Kaplan-Meier analysis. In addition, we analyzed the relationship between chromatin regulators and tumor immune infiltration, and explored differences in drug sensitivity between risk groups. RESULTS: We constructed a model consisting of 11 CRs to predict the prognosis of renal cancer patients. We not only successfully validated its feasibility, but also found that the 11 CR-based model was an independent prognostic factor. Functional analysis showed that CRs were mainly enriched in cancer development-related signalling pathways. We also found through the TIMER database that CR-based models were also associated with immune cell infiltration and immune checkpoints. At the same time, the genomics of drug sensitivity in cancer database was used to analyze the commonly used drugs of renal clear cell carcinoma patients. It was found that patients in the low-risk group were sensitive to medicines such as axitinib, pazopanib, sorafenib, and gemcitabine. In contrast, those in the high-risk group may be sensitive to sunitinib. CONCLUSION: The chromatin regulator-related prognostic model we constructed can be used to assess the prognostic risk of patients with clear cell renal cell carcinoma. The results of this study can bring new ideas for targeted therapy of clear cell renal carcinoma, helping doctors to take corresponding measures in advance for patients with different risks.

Improvement and stabilization of rice production by delaying sowing date in irrigated rice system in central China.

BACKGROUND: Climate change has posed great challenges to rice production. Temperature and solar radiation show significant variations in central China. This study aims to analyze the responses of different rice genotypes to the variations of temperature and solar radiation in central China, and to find the way of identifying the optimal sowing date to improve and stabilize rice production. For this end, four rice genotypes (two Indica and two Japonica cultivars) were cultivated at two locations under irrigation conditions in 2 years with six sowing dates. RESULTS: We investigated variations of rice grain yield, resource use efficiency, average daily temperature and solar radiation during different phenological stages. Rice grain yield could increase by about 2-17% in central China. Compared with solar radiation, temperature was a more important factor affecting rice grain yield in central China. The grain yield showed great correlation with the means temperature during different phenological stages, especially during the first 20 days after heading (GT20). Besides our results demonstrated that the grain yield displayed slender variations when the GT20 was within 24.9-26.4 °C. However, GT20 was higher than 26.4 °C in most cases, which became more frequent due to climate changes. Analysis of climate change during the last 25 years revealed that the frequency of GT20 within 24.9-26.4 °C was increased by the delay of sowing date. CONCLUSION: We propose that delaying sowing date to achieve the optimal GT20 (24.9 °C-26.4 °C) can be an effective strategy to stabilize and improve rice grain yield and resource use efficiency in central China. © 2019 Society of Chemical Industry.

MicroRNA-384-3p inhibits retinal neovascularization through targeting hexokinase 2 in mice with diabetic retinopathy.

Diabetic retinopathy (DR) presents a microvascular complication of diabetes, which may contribute to visual impairment. The treatment of DR is still controversial. Accumulating studies have reported the role of microRNAs (miRs) in DR. This study aims to explore the functions of microRNA-384-3p (miR-384-3p) in retinal neovascularization by targeting hexokinase 2 (HK2) in mice with DR. A total of 43 C57BL/6 male mice were selected and divided into normal ( n = 16) and DR ( n = 27) groups. Retinal microvascular endothelial cells (RMECs) were collected from the normal and DR mice and mainly treated with a miR-384-3p mimic, a miR-384-3p inhibitor, small interfering RNA (siRNA) against HK2 and HK2 overexpression plasmids to understand the underlying regulatory mechanisms of miR-384-3p. The relationship between miR-384-3p and HK2 was determined by dual-luciferase reporter assay. The miR-384-3p expression and the mRNA and the protein expressions of HK2 and CD31 in retinal tissues and cells were evaluated using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot assay. Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Tube formation was observed by conducting a tube formation experiment. HK2 is a target gene of miR-384-3p. The DR mice showed higher expression of HK2 and CD31 but lower expression of miR-384-3p. The miR-384-3p mimic and siRNA-HK2 reduced the expression of HK2, decreased cell proliferation and tube formation of RMECs, whereas the miR-384-3p inhibitor could reverse these trends. Our study demonstrates that overexpression of miR-384-3p inhibits retinal neovascularization in DR mice via inhibition of HK2.

Linking the branchpoint helix to a newly found receptor allows lariat formation by a group II intron.

Like spliceosomal introns, the ribozyme-containing group II introns are excised as branched, lariat structures: a 2'-5' bond is created between the first nucleotide of the intron and an adenosine in domain VI, a component which is missing from available crystal structures of the ribozyme. Comparative sequence analysis, modelling and nucleotide substitutions point to the existence, and probable location, of a specific RNA receptor for the section of domain VI that lies just distal to the branchpoint adenosine. By designing oligonucleotides that tether domain VI to this novel binding site, we have been able to specifically activate lariat formation in an engineered, defective group II ribozyme. The location of the newly identified receptor implies that prior to exon ligation, the distal part of domain VI undergoes a major translocation, which can now be brought under control by the system of anchoring oligonucleotides we have developed. Interestingly, these oligonucleotides, which link the branchpoint helix and the binding site for intron nucleotides 3-4, may be viewed as counterparts of U2-U6 helix III in the spliceosome.

High-throughput detection of common sequence variations of Fabry disease in Taiwan using DNA mass spectrometry.

BACKGROUND: In view of the therapeutic benefits resulting from early intervention for Fabry disease, our team has implemented an enzyme-based newborn screening in Taiwan since 2008. However, we found that most heterozygous females cannot be detected. To improve the screening efficiency, a more effective method for GLA gene genotyping is necessary. METHODS: As the suspected mutations are limited to only 29 different spots in Taiwanese, a panel of Sequenom iPLEX assay was designed for rapid screening of GLA variations. To determine the accuracy and sensitivity of this assay, previously diagnosed and undiagnosed DNA samples were analyzed by this genotyping assay and Sanger sequencing. In addition, DNA extracted from dried blood spots was also tested. RESULTS: Sequenom iPLEX assay is accurate and cost-effective, identifying the sequence variations, which were designated in the panel. It identified common GLA variants in DNA samples extracted from whole blood or dried blood spots with 100% accuracy and sensitivity. CONCLUSIONS: Sequenom iPLEX assay is suitable for Fabry newborn screening when hotspot mutations and common variations are known in a well-studied population. In addition, this assay can also be applied for first-line determination of GLA variant sequences in suspected subjects of high-risk patients, or newborns.

The effects of rape residue mulching on net global warming potential and greenhouse gas intensity from no-tillage paddy fields.

A field experiment was conducted to provide a complete greenhouse gas (GHG) accounting for global warming potential (GWP), net GWP, and greenhouse gas intensity (GHGI) from no-tillage (NT) paddy fields with different amounts of oilseed rape residue mulch (0, 3000, 4000, and 6000 kg dry matter (DM) ha(-1)) during a rice-growing season after 3 years of oilseed rape-rice cultivation. Residue mulching treatments showed significantly more organic carbon (C) density for the 0-20 cm soil layer at harvesting than no residue treatment. During a rice-growing season, residue mulching treatments sequestered significantly more organic C from 687 kg C ha(-1) season(-1) to 1654 kg C ha(-1) season(-1) than no residue treatment. Residue mulching significantly increased emissions of CO2 and N2O but decreased CH4 emissions. Residue mulching treatments significantly increased GWP by 9-30% but significantly decreased net GWP by 33-71% and GHGI by 35-72% relative to no residue treatment. These results suggest that agricultural economic viability and GHG mitigation can be achieved simultaneously by residue mulching on NT paddy fields in central China.

Comprehensive impacts of different integrated rice-animal co-culture systems on rice yield, nitrogen fertilizer partial factor productivity and nitrogen losses: A global meta-analysis.

Integrated rice-animal co-culture (IRAC) is an ecological agricultural system combining rice cultivation with animal farming, which holds significant implications for food security and agriculture sustainable development. However, the comprehensive impacts of the co-culture on rice yield, nitrogen (N) losses, and N fertilizer partial factor productivity (NPFP) remain elusive and may vary under different environmental conditions and N management. Here, we conducted a meta-analysis of data from various IRAC systems on a global scale, including 371, 298, and 115 sets of data for rice yield, NPFP, and N losses, respectively. The results showed that IRAC could significantly increase rice yield (by 3.47 %) and NPFP (by 4.26 %), and reduce N2O emissions (by 16.69 %), NH3 volatilization (by 11.03 %), N runoff (by 17.72 %), and N leaching (by 19.10 %). Furthermore, there were significant differences in rice yield, NPFP, and N loss among different IRAC systems, which may be ascribed to variations in regional climate, soil variables, and N fertilizer management practices. The effect sizes of rice yield and NPFP were notably correlated with the rate and frequency of N application and the soil clay content. Moreover, a higher amount of precipitation corresponded to a larger effect size on rice NPFP. N2O emissions were closely associated with mean annual air temperature, annual precipitation, N application frequency, soil pH level, soil organic matter content, soil clay content, and soil bulk density. However, NH3 volatilization, N runoff, and N leaching exhibited no correlation with either the environmental conditions or the N management. Multivariate regression analysis further demonstrated that the soil clay content and N application rate are pivotal in predicting the effect sizes of rice yield, NPFP, and N2O emissions under IRAC. Specifically, IRAC with a low N application rate in soils with a high clay content could augment the effect size to increase rice NPFP and yield and reduce N2O emissions. In conclusion, IRAC offers a potent strategy to optimize rice yield and NPFP as well as mitigate N losses.

Decoding Bilingual EEG Signals With Complex Semantics Using Adaptive Graph Attention Convolutional Network.

Decoding neural signals of silent reading with Brain-Computer Interface (BCI) techniques presents a fast and intuitive communication method for severely aphasia patients. Electroencephalogram (EEG) acquisition is convenient and easily wearable with high temporal resolution. However, existing EEG-based decoding units primarily concentrate on individual words due to their low signal-to-noise ratio, rendering them insufficient for facilitating daily communication. Decoding at the word level is less efficient than decoding at the phrase or sentence level. Furthermore, with the popularity of multilingualism, decoding EEG signals with complex semantics under multiple languages is highly urgent and necessary. To the best of our knowledge, there is currently no research on decoding EEG signals during silent reading of complex semantics, let alone decoding silent reading EEG signals with complex semantics for bilingualism. Moreover, the feasibility of decoding such signals remains to be investigated. In this work, we collect silent reading EEG signals of 9 English Phrases (EP), 7 English Sentences (ES), 10 Chinese Phrases (CP), and 7 Chinese Sentences (CS) from the subject within 26 days. We propose a novel Adaptive Graph Attention Convolution Network (AGACN) for classification. Experimental results demonstrate that our proposed method outperforms state-of-the-art methods, achieving the highest classification accuracy of 54.70%, 62.26%, 44.55%, and 57.14% for silent reading EEG signals of EP, ES, CP, and CS, respectively. Moreover, our results prove the feasibility of complex semantics EEG signal decoding. This work will aid aphasic patients in achieving regular communication while providing novel ideas for neural signal decoding research.

Causal relationship between several autoimmune diseases and renal malignancies: A two-sample mendelian randomization study.

OBJECTIVE: Observational studies have shown an association between systemic autoimmune disease (AD) and multiple malignancies. However, due to the difficulty indetermining the temporal nature of the order, their causal relationship remains elusive. Based on pooled data from a large population-wide genome-wide association study (GWAS), this study explores the genetic causality between systemic autoimmune disease and renal malignancy. METHODS: We took a series of quality control steps from a large-scale genome-wide association study to select single nucleotide polymorphisms (SNPs) associated with systemic autoimmune disease as instrumental variables(IVs) to analyze genetic causality with renal malignancies. Inverse variance weighting (IVW), MR- Egger, weighted median, simple model and weighted model were used for analysis. The results were mainly based on IVW (Random Effects), followed by sensitivity analysis. Inverse-Variance Weighted(IVW) and MR-Egger were used to test for heterogeneity. MR- Egger is also used for pleiotropic testing. A single SNP analysis was used to identify single nucleotide polymorphisms (SNPs) with potential impact. Odds ratio (OR) and 95% confidence interval (CI) were used to evaluate causality, and sensitivity analysis was performed to evaluate pleiotropy and instrumental validity. RESULTS: Acute and subacute iridocylitis (P = 0.006, OR = 1.077), Ankylosing spondylitis (P = 0.002, OR = 1.051), and spondyloarthritis (P = 0.009, OR = 1.073) were positively associated with an increased risk of renal malignancy. Coxarthrosis (P = 0.008, OR = 0.483), Juvenile rheumatism (P = 0.011, OR = 0.897), and Systemic lupus erythematosus (P = 0.014, OR = 0.869) were negatively associated with an increased risk of renal malignancy. The results of sensitivity analysis were consistent without heterogeneity or pleiotropy. CONCLUSION: Our study suggests a causal relationship between different systemic autoimmune diseases and renal malignancies. These findings prompt health care providers to take seriously the potential risk of systemic autoimmune disease and provide new insights into the genetics of kidney malignancies.

2D Z-scheme ZnIn2S4/g-C3N4 heterojunction based on photoelectrochemical immunosensor with enhanced carrier separation for sensitive detection of CEA.

Semiconducting materials based on photoelectrochemical (PEC) sensors have been widely utilized for detection. Meanwhile, the sensitivity of the PEC sensor was limited by low-efficiency carrier separation. Thus, a novel sandwich-type PEC bioimmunosensing based on 2D Z-scheme ZnIn2S4/g-C3N4 heterojunction as a photosensitive material and BiVO4 as a photoquencher was designed for the sensitive detection of carcinoembryonic antigen (CEA). Firstly, the 2D ZnIn2S4/g-C3N4 structure provided a multitude of activated sites which facilitated the loading of the capture antibody (Ab1). Secondly, the Z-scheme heterojunction had a high redox capacity while promoting the rapid separation and migration of photogenerated electron-hole pairs (e-/h+). Thus it was able to consume more electron donors to a certain extent, resulting in a higher initial photocurrent. In addition, BiVO4 with large spatial potential resistance was introduced for the first time to realize signal amplification. BiVO4 could not only compete with substrate materials for electron donors, but also effectively prevent electron donors from contacting the substrate, further reducing the photocurrent signal. Under optimized conditions, the sensor had a favorable detection range (0.0001-100 ng/mL) to CEA and a low detection limit of 0.03 pg/mL. With high specificity, excellent stability, and remarkable reproducibility, this sensor provided a new perspective for constructing accurate and convenient PEC immunosensor for bioanalysis and early disease diagnosisdisease diagnosis.

Recurrent insertion of 5'-terminal nucleotides and loss of the branchpoint motif in lineages of group II introns inserted in mitochondrial preribosomal RNAs.

A survey of sequence databases revealed 10 instances of subgroup IIB1 mitochondrial ribosomal introns with 1 to 33 additional nucleotides inserted between the 5' exon and the consensus sequence at the intron 5' end. These 10 introns depart further from the IIB1 consensus in their predicted domain VI structure: In contrast to its basal helix and distal GNRA terminal loop, the middle part of domain VI is highly variable and lacks the bulging A that serves as the branchpoint in lariat formation. In vitro experiments using two closely related IIB1 members inserted at the same ribosomal RNA site in the basidiomycete fungi Grifola frondosa and Pycnoporellus fulgens revealed that both ribozymes are capable of efficient self-splicing. However, whereas the Grifola intron was excised predominantly as a lariat, the Pycnoporellus intron, which possesses six additional nucleotides at the 5' end, yielded only linear products, consistent with its predicted domain VI structure. Strikingly, all of the introns with 5' terminal insertions lack the EBS2 exon-binding site. Moreover, several of them are part of the small subset of group II introns that encode potentially functional homing endonucleases of the LAGLIDADG family rather than reverse transcriptases. Such coincidences suggest causal relationships between the shift to DNA-based mobility, the loss of one of the two ribozyme sites for binding the 5' exon, and the exclusive use of hydrolysis to initiate splicing.

Integrated rice-crayfish farming system does not mitigate the global warming potential during rice season.

Integrated rice-crayfish farming system (RCS) has become increasingly popular in China. However, previous research has largely ignored the effect of trench around the paddy field on GHG emissions, which may cause inaccurate estimation of the global warming potential (GWP) from the system. This study compared the GWP between rice monoculture (RM) and RCS. The results indicated that the field of RCS had significantly lower CH4 emissions compared with RM due to lower mcrA abundance and higher pmoA abundance, while there was no difference in N2O emissions. In addition, the trench resulted in remarkably more CH4 emissions due to higher mcrA abundance and lower pmoA abundance and less N2O emissions than the field in RCS. In general, RCS seems not to mitigate GWP compared with RM due to more CH4 emissions from the trench in the current mode. Furthermore, our results indicate that RCS can reduce GWP relative to RM only when the area ratio of the trench to the system is controlled to be lower than 13.9 %.

Rethinking Saliency Map: A Context-Aware Perturbation Method to Explain EEG-Based Deep Learning Model.

Deep learning is widely used to decode the electroencephalogram (EEG) signal. However, there are few attempts to specifically study how to explain EEG-based deep learning models. In this paper, we review the related works that attempt to explain EEG-based models. And we find that the existing methods are not perfect enough to explain the EEG-based model due to the non-stationary nature, high inter-subject variability and dependency of EEG data. The characteristics of the EEG data require the explanation to incorporate the instance-level saliency identification and the context information of EEG data. Recently, mask perturbation is proposed to explain deep learning model. Inspired by the mask perturbation, we propose a new context-aware perturbation method to address these concerns. Our method not only extends the scope to the instance level but can capture the representative context information when estimating the saliency map. In addition, we point out another role of context information in explaining the EEG-based model. The context information can also help suppress the artifacts in the EEG-based deep learning model. In practice, some users might want a simple version of the explanation, which only indicates a few features as salient points. To further improve the usability of our method, we propose an optional area limitation strategy to restrict the highlighted region. In the experiment section, we select three representative EEG-based models and implement them on the emotional EEG dataset DEAP. The results of the experiments support the advantages of our method.

Endometriosis of the lung: A case report and review of literature.

BACKGROUND: Lung endometriosis is an extremely rare gynecological disease. Current literature reports suggest that the majority of patients will present with only generic symptoms, such as hemoptysis, pneumothorax, and hemopneumothorax, which often leads to misdiagnosis. To date, there are 18 case reports of lung endometriosis that describe the clinical manifestation, imaging changes, treatment, and prognosis of the disease. To provide further insights into this rare disease, we present a new case report and a brief review of pulmonary endometriosis. CASE SUMMARY: We report here about a 19-year-old woman who was admitted to the hospital for repeated catamenial hemoptysis over a 3-mo period. computed tomography (CT) imaging during menstruation revealed patchy high-density shadows, approximately 0.5 cm3 in size, in the right middle lobe of the lung. The patient's hemoptysis and changes in the CT scans resolved after menstruation. Thoracoscopic right middle lobectomy, right lower lung repair, and closed thoracic drainage were performed. Postoperative histopathology confirmed lung endometriosis. There was no recurrence of symptoms at the 6 mo follow-up. CONCLUSION: We propose diagnosing lung endometriosis by thoroughly taking reproductive history, clinical details, imaging, and histopathology followed by treatment with surgical resection.

Near-infrared light-induced photoelectrochemical biosensor based on plasmon-enhanced upconversion nanocomposites for microRNA-155 detection with cascade amplifications.

Herein, a novel near-infrared (NIR) light-driven photoelectrochemical (PEC) biosensor based on NaYF4:Yb3+, Er3+@Bi2MoO6@Bi (NYF@BMO@Bi) nanocomposites was elaborately developed to achieve highly sensitive detection of microRNA-155 (miRNA-155). To realize signal enhancement, the coupled plasmonic bismuth (Bi) nanoparticles were constructed as an energy relay to facilitate the transfer of energy from NaYF4:Yb3+, Er3+ to Bi2MoO6, ultimately enabling the efficient separation of electron-hole pairs of Bi2MoO6 under the irradiation of a 980 nm laser. For constructing biosensing system, the initial signal was firstly amplified after the addition of alkaline phosphatase (ALP) in conjunction with the biofunctionalized NYF@BMO@Bi nanocomposites, which could catalyze the conversion of ascorbic acid 2-phosphate into ascorbic acid, and then consumed the photoacoustic holes created on the surface of Bi2MoO6 for the enlarging photocurrent production. Upon addition of target miRNA-155, the cascade signal amplification process was triggered while the ALP-modified DNA sequence was replaced and then followed by the initiation of a simulated biocatalytic precipitation reaction to attenuate the photocurrent response. On account of the NIR-light-driven and cascade amplifications strategy, the as-constructed biosensor was successfully utilized for the accurate determination of miRNA-155 ranging from 1 fM to 0.1 µM with a detection limit of 0.32 fM. We believed that the proposed nanocomposites-based NIR-triggered PEC biosensor could provide a promising platform for effective monitoring other tumor biomarkers in clinical diagnostics.

Response of Source-Sink Characteristics and Rice Quality to High Natural Field Temperature During Reproductive Stage in Irrigated Rice System.

Global warming greatly affects the development of rice at different growth stages, thereby deteriorating rice quality. However, the effect of high natural field temperature during reproductive stages on rice quality is unclear. Thus, grain filling dynamics, source-sink characteristics and quality-related traits were compared between two contrasting natural field temperature conditions namely normal (low temperature) (LRT) and hot (high temperature) growth season (HRT) during reproductive stage. Compared with LRT, HRT significantly increased chalky grain rate (about 1.6-3.1%), chalkiness level (about 4.7-22.4%), protein content (about 0.93-1.07%), pasting temperature, setback, and consistence, and decreased total starch content (about 4.6-6.2%). Moreover, HRT significantly reduced the leaf area index (LAI, about 0.54-1.11 m2 m-2), specific leaf weight (SLW, about 1.27-1.44 mg cm-2) and source-sink ratio (leaf-sink ratio and/or stem-sink ratio), shortened the active grain filling period by 3.1-3.2 days, and reduced the rations of dry matter translocation to grain (RDMs). In conclusion, we suggested that significant reduction in assimilate translocation after flowering, resulting in the reduced active grain-filling duration and the poor rice quality (high chalkiness and the poor eating and cooking quality), modulated by source-sink characteristics in response to high natural field temperature during reproductive stage. These results enriched the study of high temperature-stressed rice and served as an important reference for selecting high-quality, heat-tolerant varieties and protecting rice quality under high-temperature conditions.

Multiple primary lung cancer comprised of adenocarcinoma and adenoid cystic carcinoma: a case report.

Background: Multiple primary lung cancer (MPLC) is a rare type of tumor, and it is necessary to differentiate it from a metastatic tumor. The type of adenocarcinoma with adenoid cystic carcinoma (ACC) is extremely rare and has not yet been reported in the literature. The initial clinical symptoms of double primary lung cancer may be nonspecific; hence, the diagnosis is often missed or incorrect. Case Description: In this case presentation, we report the case of a 67-year-old female who had experienced persistent cough and expectoration for 8 days. Chest computed tomography (CT) revealed 2 nodules in the patient's lung. Radiographic findings could not distinguish between the 2 nodules and between primary and metastatic lesions. Thus, the patient underwent bronchoscopic biopsy and percutaneous lung puncture. We could not determine the type of the two tumors in hematoxylin and eosin (H&E) staining sections, and we subsequently performed specific immunohistochemical (IHC) staining. Combined with morphological and IHC results, we concluded that this was a case of MPLC, consisting of adenocarcinoma and ACC. The patient received symptomatic treatment because of the metastases. Conclusions: This report reports a rare combination of MPLC and shows that a definitive diagnosis of double primary lung cancer can be based on tissue biopsy and IHC techniques.

A case of death of patient with ovarian fibroma combined with Meigs Syndrome and literature review.

Ovarian fibroma is the most common benign pure stromal tumor. It has no specific clinical manifestation, most of which are pelvic or adnexal masses. 10-15% of cases with hydrothorax or ascites, after tumor resection, hydrothorax and ascites disappear, known as Meigs Syndrome. The elevated level of CA125 in a few patients was easily misdiagnosed as ovarian malignant tumor. A case of bilateral Ovarian fibroma associated with Meigs Syndrome is reported and the literature is reviewed in order to improve the understanding of the changes and avoid misdiagnosis.

Integrated effects of microbial decomposing inoculant on greenhouse gas emissions, grain yield and economic profit from paddy fields under different water regimes.

Few studies have comprehensively evaluated the impacts of microbial decomposing inoculants on greenhouse gas emissions and economic profit from paddy fields under different water regimes. Here, this study evaluated the effects of microbial decomposing inoculant treatments (straw returning without or with microbial decomposing inoculants (S and SMD)) on rice yield, CH4 and N2O emissions, economic profit and net ecosystem economic profit (NEEP) from paddy fields under different water regimes (continuous flooding (CF) and alternate wetting and drying irrigation (AWD)) in central China with a two-year field experiment. Compared with S treatment, SMD treatment significantly increased the rice yield and crop water productivity by 6.6-7.2% and 5.6-7.9%, respectively. AWD treatment significantly enhanced the crop water productivity by 56.9-73.7% while did not affect rice yield relative to CF treatment. Regardless of water regimes, SMD treatment did not affect N2O emissions, but significantly increased CH4 emissions by 13.8-39.6% relative to S treatment, resulting in a remarkable enhancement of global warming potential by 13.5-32.5%. Compared with S treatment, SMD treatment improved the economic profit and NEEP. By contrast, AWD treatment significantly increased N2O emissions by 19.1-64.8% compared with CF treatment, but significantly reduced CH4 emissions by 35.3-79.1%. Accordingly, AWD treatment significantly decreased the global warming potential by 33.4-73.9% compared with CF treatment. In addition, AWD treatment resulted in 39.9-96.4% higher economic profit and 48.0-124.4% higher NEEP relative to CF treatment. In summary, AWD treatment is a sustainable water regime that can maintain rice yield, mitigate global warming potential, and increase economic income. However, regardless of water regimes, SMD treatment led to higher rice yield and economic profit, as well as higher global warming potential than S treatment, suggesting that other appropriate treatments of crop straw are needed to mitigate CH4 emissions while improving economic profit for rice sustainable production.

Early hepatoid adenocarcinoma of the stomach with signet ring cell carcinoma: A case report and clinicopathological features.

Background: Hepatoid adenocarcinoma of the stomach (HAS) is a rare subtype of gastric cancer with poor prognosis, and its clinicopathological features are not well understood, so the pathology from the clinical biopsy is easily misdiagnosed, especially for special or atypical HAS. We present an extremely rare early HAS with signet ring cell carcinoma and evaluate its clinicopathological features. Case presentation: A 51-year-old female patient of Chinese Han ethnicity with upper abdominal pain for 5 years and worsened abdominal pain for 1 month was admitted to our hospital. Esophagogastroduodenoscopy showed a submucosal tumor-like elevated lesion with central depression in the greater curvature of the junction between the antrum and body. Histopathological examination from the biopsy revealed medium-low-differentiation adenocarcinoma with signet ring cell carcinoma. Radical gastrectomy was performed, and the final diagnosis was early HAS with signet ring cell carcinoma. Conclusions: HAS with signet ring cell carcinoma is a special type of HAS and extremely rare. It is first presented for this extremely rare type of HAS, which contributes to strengthen the understanding for the clinicopathological characteristics of HAS and especially promote early detection of HAS.