Anti-Inflammatory and Analgesic Effects of the Marine-Derived Compound Excavatolide B Isolated from the Culture-Type Formosan Gorgonian Briareum excavatum.

In recent years, several marine-derived compounds have been clinically evaluated. Diterpenes are secondary metabolites from soft coral that exhibit anti-inflammatory, anti-tumor and cytotoxic activities. In the present study, we isolated a natural diterpene product, excavatolide B, from cultured Formosan gorgonian Briareum excavatum and investigated its anti-inflammatory activities. We found that excavatolide B significantly inhibited the mRNA expression of the proinflammatory mediators, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in lipopolysaccharide (LPS)-challenged murine macrophages (RAW 264.7). We also examined the anti-inflammatory and anti-nociceptive effects of excavatolide B on intraplantar carrageenan-induced inflammatory responses. Excavatolide B was found to significantly attenuate carrageenan-induced nociceptive behaviors, mechanical allodynia, thermal hyperalgesia, weight bearing deficits and paw edema. In addition, excavatolide B inhibited iNOS, as well as the infiltration of immune cells in carrageenan-induced inflammatory paw tissue.

Removal of chlorpheniramine and variations of nitrosamine formation potentials in municipal wastewaters by adsorption onto the GO-Fe3O4.

Chlorpheniramine is a pharmaceutical pollutant and a precursor of carcinogenic nitrosamines during disinfection/oxidation. In our previous study, graphene oxide coated with magnetite (GO-Fe3O4) was capable of removing chlorpheniramine in deionized water by adsorption. This study investigated the removal of chlorpheniramine and its nitrosamine formation potentials (FPs) by adsorption onto magnetic GO-Fe3O4, with respect to the influence by using real municipal wastewaters as the background. In the results, the adsorption performances of chlorpheniramine in wastewaters decreased in the order: GO-Fe3O4 suspension > GO-Fe3O4 particles > activated carbon. Chlorpheniramine adsorptions on GO-Fe3O4 particles and activated carbon were reduced by using real wastewaters as the background, whereas chlorpheniramine adsorption on GO-Fe3O4 suspension was enhanced due to the effects of surface charge on GO-Fe3O4 and ionic strength variation in water. The fittings of adsorption isotherms indicated that the wastewater background reduced the surface heterogeneity of GO-Fe3O4 suspension and improved the adsorption performance. Appreciable removal efficiencies of NDMA and other nitrosamine FPs were observed when GO-Fe3O4 particles were added in real wastewaters. However, when chlorpheniramine was present in wastewaters, chlorpheniramine adsorption and degradation reaction simultaneously occurred on the surface of GO-Fe3O4, increasing NDMA and other nitrosamine FPs in wastewaters after GO-Fe3O4 addition for chlorpheniramine adsorption. The assumption was further demonstrated by observing the NDMA-FP increase during chlorpheniramine adsorption on GO-Fe3O4 in deionized water. GO-Fe3O4 is a potential adsorbent for chlorpheniramine removal. Nevertheless, the low treatment efficiencies at high doses limit its application for nitrosamine FP adsorptions in real wastewaters.

Effect of molecular characteristics on the formation of nitrosamines during chlor(am)ination of phenylurea herbicides.

The objective of this study was to investigate the formation of different nitrosamines during chlorination or chloramination (chlor(am)ination) of five phenylurea herbicides (fluometuron, diuron, linuron, metobromuron, and propanil), with the effects of disinfection approaches, additional inorganic nitrogen, and reaction pH being studied. By analyzing six nitrosamines, N-nitrosodimethylamine (NDMA) and N-nitrosopyrrolidine (NPYR) formation was observed. The dimethylamine functional group was the key to determining whether a particular phenylurea herbicide was an important nitrosamine precursor, as the NDMA conversion ratio was much higher. Chlorination with ammonium or dichloramination enhanced the NDMA formation. NPYR formation from the herbicides that did not form NDMA was detected and was more vigorous during dichloramination or in the presence of either ammonium or nitrite. The NPYR formation was possibly related to the aniline molecular fragment from the phenylurea herbicides. Both NDMA and NPYR formation were higher at pH 8. Overall, the maximum nitrosamine conversions decreased in the order: fluometuron > diuron > propanil > metobromuron > linuron (up to 0.99%, 0.46%, 0.005%, 0.004%, and 0.003% molar conversion rates, respectively) during chlorination or chloramination and dichloramine > free chlorine > monochloramine (up to 0.99%, 0.41%, and 0.005% molar conversion rates, respectively) for given herbicide, chlorine, and nitrogen doses. Applying the results of this study, phenylurea herbicide concentrations ranging from several to tens of µg L(-1) will yield a NDMA concentration in drinking water above the level for a theoretical 10(-6) lifetime cancer risk. NPYR formation will increase the risk of these phenylurea herbicide concentrations to downstream water users. The true adverse environmental impacts of these phenylurea herbicides are important to emphasize given their high loadings as non-point source pollutants and their typical environmental scenarios (e.g., at neutral pH or with the co-occurrence of inorganic nitrogen), likely resulting in more efficient nitrosamine formation.