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1.
Org Lett ; 19(6): 1318-1321, 2017 03 17.
Artigo em Inglês | MEDLINE | ID: mdl-28233489

RESUMO

The highly enantioselective synthesis of 4-aryl-3,4-dihydrocoumarins was realized through direct annulation of phenols with enals catalyzed by dihydroisoquinoline-type NHC (DHIQ-NHC), an N-heterocyclic carbene derived from l-phenylalanine. The catalytic reaction proceeds with a wide scope of electron-rich phenols and enals providing structurally diverse 4-aryl-3,4-dihydrocoumarins in good to excellent yields and enantioselectivity. This method was useful in the synthesis of natural products and biologically relevant compounds from readily available starting materials.

2.
Chem Sci ; 6(7): 4273-4278, 2015 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-29218195

RESUMO

A series of chiral triazolium salts have been synthesized from methyl l-phenylalaninate hydrochloride. The NHCs derived from this class of novel triazolium salts were found to be highly efficient catalysts in the annulation reaction of enals and 2-naphthols. These reactions proceeded with high chemoselectivity and wide substrate scope affording enantioenriched ß-arylsplitomicins in good yields with up to 96% ee.

4.
Artigo em Zh | MEDLINE | ID: mdl-20108765

RESUMO

OBJECTIVE: To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene. METHODS: Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4. RESULTS: The six JEV strains were clustered in genotype I. 87.5%-87.9% identity in nucleotide sequence and 96.8%-97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain. CONCLUSION: Genotype I JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.


Assuntos
Vírus da Encefalite Japonesa (Espécie)/genética , Vírus da Encefalite Japonesa (Espécie)/isolamento & purificação , Encefalite Japonesa/virologia , Sequência de Aminoácidos , Animais , Linhagem Celular , China , Cricetinae , Vírus da Encefalite Japonesa (Espécie)/química , Vírus da Encefalite Japonesa (Espécie)/classificação , Humanos , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Proteínas Virais/química , Proteínas Virais/genética
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