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1.
Beilstein J Org Chem ; 10: 858-862, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24778741

RESUMO

Two pairs of geometrical isomers - cuevaenes A (1) and C (3) as well as cuevaenes D (4) and E (5) - and cuevaene B (2) were isolated from gdmAI-disrupted Streptomyces sp. LZ35. The constitution of cuevaene C (3) was found to be identical to cuevaene A (1) by means of NMR spectroscopy and high resolution mass spectrometry. However, the relative configurations of the triene side chain moieties were determined to be different. It was established on the basis of spectroscopic data that cuevaenes D (4) and E (5) are amides and geometrical isomers. Cuevaenes A-C (1-3) displayed moderate activity against Gram-positive bacteria (e.g., Bacillus subtilis strain ATCC 11060) and modest activity against fungi (e.g., Fusarium verticillioides strain S68 and Rhizoctonia solani strain GXE4). However, cuevaenes D (4) and E (5) showed no inhibitory activity against any of the tested microbes.

2.
Chin J Nat Med ; 14(12): 931-933, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28262120

RESUMO

A new hexaketide acid esterified by the 17-hydroxyl group of 16,17-dihydroxycyclooctatin, namely 17-[16,17-dihydroxycyclooctatinyl]-hexaketide ester (1), a member of the group of rare bacterial diterpenes with a fused 5-8-5 ring system was isolated from strain Streptomyces sp. SR107. The structure was determined on the basis of its spectral data (1H NMR, 13C NMR, 1H-1H COSY, HSQC, HMBC, NOESY, IR and HR-ESI-MS). The antibacterial activity was also evaluated in this paper.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Diterpenos/química , Diterpenos/farmacologia , Streptomyces/química , Bactérias/efeitos dos fármacos , Diterpenos/metabolismo , Ésteres/química , Ésteres/farmacologia , Espectroscopia de Ressonância Magnética , Estrutura Molecular
3.
Gene ; 544(1): 93-9, 2014 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-24768719

RESUMO

Divergolides are a group of structurally unprecedented ansamacrolactam antibiotics with antibacterial and antitumor activities. A biosynthetic gene cluster predicted to encode the biosynthesis of divergolides was cloned and sequenced from endophytic Streptomyces sp. W112. The gene cluster of divergolides (div) spans a DNA region of 61-kb and consists of 20 open reading frames (ORFs) that encode polyketide synthases (PKSs), enzymes for the synthesis of AHBA and PKS extender units, and post-PKS modifications, proposed regulators, and putative transporters. Disruption of the AHBA synthase gene (divK) completely abolished the production of divergolides proved its involvement in the biosynthesis of divergolides. Bioinformatics analysis suggested that the regulatory gene div8 in div gene cluster might encode a positive regulator for the biosynthesis of divergolides. Constitutive overexpression of div8 improved the production of divergolides E, implying that div gene cluster maybe responsible for the biosynthesis of divergolides. These findings set the stage for fully investigating the biosynthesis of divergolides and rational engineering of new divergolide analogs by genetic modifications, and pave the way to further improve the production of divergolides.


Assuntos
Proteínas de Bactérias/genética , Macrolídeos/metabolismo , Família Multigênica , Streptomyces/genética , Aminobenzoatos/metabolismo , Antibacterianos/biossíntese , Antibacterianos/química , Proteínas de Bactérias/metabolismo , Vias Biossintéticas/genética , Clonagem Molecular , Regulação Bacteriana da Expressão Gênica , Biblioteca Genômica , Hidroliases/genética , Hidroliases/metabolismo , Hidroxibenzoatos/metabolismo , Macrolídeos/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Fases de Leitura Aberta/genética , Policetídeo Sintases/genética , Policetídeo Sintases/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Espectrometria de Massas por Ionização por Electrospray , Streptomyces/metabolismo
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