Integrated behavioural and physiological responses of sand smelt larvae to the effects of warming and hypoxia as combined stressors.

Forecasts indicate that rising temperatures towards the future and the expansion of dead zones will change environmental suitability for fish early stages. Therefore, we assessed the chronic effects of warming (26 °C), hypoxia (<2-2.5 mg L-1) or their combination on mortality rate, growth, behaviour, energy metabolism and oxidative stress using Atherina presbyter larvae as a model species. There were no differences between the treatments in terms of mortality rate. The combination of warming and hypoxia induced faster loss of body mass (+22.7%). Warming, hypoxia or their combination enhanced boldness (+14.7-25.4%), but decreased exploration (-95%-121%), increased the time in frozen state (+60.6-80.5%) and depleted swimming speed (-45.6-50.5%). Moreover, routine metabolic rate was depleted under hypoxia or under the combination of warming and hypoxia (-56.6 and 57.2%, respectively). Under hypoxia, increased catalase activity (+56.3%) indicates some level of antioxidant defence capacity, although increased DNA damage (+25.2%) has also been observed. Larvae also exhibited a great capacity to maintain the anaerobic metabolism stable in all situations, but the aerobic metabolism is enhanced (+19.3%) when exposed to the combination of both stressors. The integrative approach showed that changes in most target responses can be explained physiologically by oxidative stress responses. Increased oxidative damages (lipid peroxidation and DNA damage) and increased interaction between antioxidant enzymes (superoxide dismutase and catalase) are associated to increased time in frozen state and decreased swimming activity, growth rates and boldness. Under all stressful situations, larvae reduced energy-consuming behaviours (e.g. depleted exploration and swimming activity) likely to stabilize or compensate for the aerobic and anaerobic metabolisms. Despite being an active small pelagic fish, we concluded that the sensitive larval phase exhibited complex coping strategies to physiologically acclimate under thermal and hypoxic stress via behavioural responses.

Early life stage mechanisms of an active fish species to cope with ocean warming and hypoxia as interacting stressors.

Ocean's characteristics are rapidly changing, modifying environmental suitability for early life stages of fish. We assessed whether the chronic effects of warming (24 °C) and hypoxia (<2-2.5 mg L-1) will be amplified by the combination of these stressors on mortality, growth, behaviour, metabolism and oxidative stress of early stages of the white seabream Diplodus sargus. Combined warming and hypoxia synergistically increased larval mortality by >51%. Warming induced faster growth in length and slower gains in weight when compared to other treatments. Boldness and exploration were not directly affected, but swimming activity increased under all test treatments. Under the combination of warming and hypoxia, routine metabolic rate (RMR) significantly decreases when compared to other treatments and shows a negative thermal dependence. Superoxide dismutase and catalase activities increased under warming and were maintained similar to control levels under hypoxia or under combined stressors. Under hypoxia, the enzymatic activities were not enough to prevent oxidative damages as lipid peroxidation and DNA damage increased above control levels. Hypoxia reduced electron transport system activity (cellular respiration) and isocitrate dehydrogenase activity (aerobic metabolism) below control levels. However, lactate dehydrogenase activity (anaerobic metabolism) did not differ among treatments. A Redundancy Analysis showed that ∼99% of the variability in mortality, growth, behaviour and RMR among treatments can be explained by molecular responses. Mortality and growth are highly influenced by oxidative stress and energy metabolism, exhibiting a positive relationship with reactive oxygen species and a negative relationship with aerobic metabolism, regardless of treatment. Under hypoxic condition, RMR, boldness and swimming activity have a positive relationship with anaerobic metabolism regardless of temperature. Thus, seabreams may use anaerobic reliance to counterbalance the effects of the stressors on RMR, activity and growth. The outcomes suggests that early life stages of white seabream overcame the single and combined effects of hypoxia and warming.

Forecasting shifts in habitat suitability across the distribution range of a temperate small pelagic fish under different scenarios of climate change.

Climate change often leads to shifts in the distribution of small pelagic fish, likely by changing the match-mismatch dynamics between these sensitive species within their environmental optima. Using present-day habitat suitability, we projected how different scenarios of climate change (IPCC Representative Concentration Pathways 2.6, 4.5 and 8.5) may alter the large scale distribution of European sardine Sardina pilchardus (a model species) by 2050 and 2100. We evaluated the variability of species-specific environmental optima allowing a comparison between present-day and future scenarios. Regardless of the scenario, sea surface temperature and salinity and the interaction between current velocity and distance to the nearest coast were the main descriptors responsible for the main effects on sardine's distribution. Present-day and future potential "hotspots" for sardine were neritic zones (<250 km) with water currents <0.4 m s-1, where SST was between 10 and 22 °C and SSS > 20 (PSU), on average. Most variability in projected shifts among climatic scenarios was in habitats with moderate to low suitability. By the end of this century, habitat suitability was projected to increase in the Canary Islands, Iberian Peninsula, central North Sea, northern Mediterranean, and eastern Black Sea and to decrease in the Atlantic African coast, southwest Mediterranean, English Channel, northern North Sea and Western U.K. A gradual poleward-eastward shift in sardine distribution was also projected among scenarios. This shift was most pronounced in 2100 under RCP 8.5. In that scenario, sardines had a 9.6% range expansion which included waters along the entire coast of Norway up and into the White Sea. As habitat suitability is mediated by the synergic effects of climate variability and change on species fitness, it is critical to apply models with robust underlying species-habitat data that integrate knowledge on the full range of processes shaping species productivity and distribution.

Global patterns for the spatial distribution of floating microfibers: Arctic Ocean as a potential accumulation zone.

Despite their representativeness, most studies to date have underestimated the amount of microfibers (MFs) in the marine environment. Therefore, further research is still necessary to identify key processes governing MF distribution. Here, the interaction among surface water temperature, salinity, currents and winds explained the patterns of MF accumulation. The estimated density of floating MFs is ∼5900 ±â€¯6800 items m-3 in the global ocean; and three patterns of accumulation were predicted by the proposed model: (i) intermediate densities in ocean gyres, Seas of Japan and of Okhotsk, Mediterranean and around the Antarctic Ocean; (ii) high densities in the Arctic Ocean; and (iii) point zones of highest densities inside the Arctic Seas. Coastal areas and upwelling systems have low accumulation potential. At the same time, zones of divergences between westerlies and trade winds, located above the tropical oceanic gyres, are predicted to accumulate MFs. In addition, it is likely that the warm branch of the thermohaline circulation has an important role in the transport of MFs towards the Arctic Ocean, emphasizing that surface water masses are important predictors. This study highlights that the Arctic Ocean is a dead end for floating MFs.

Estuarine ecocline function and essential habitats for fish larvae in tropical South Western Atlantic estuaries.

Estuarine gradients rule the dispersal of larval fishes leading to community replenishment and the recruitment of juveniles to adult populations. Here, the variations in density and diversity of fish larvae communities were assessed to understand whether the seasonal variability of environmental forcings in two tropical estuaries express the estuarine function for larvae. Spatial differences ruled larval dispersal. Larval recruitment to the Caeté Estuary occurs in the upper estuary in the late-dry season. Species richness is higher when temperature, salinity and precipitation increase, while changes in diversity is more pronounced in the lower estuary due to salinity variability. Larval recruitment to the Goiana Estuary occurs in the lower estuary, with peaks during wet warmer conditions. Species richness and diversity are also higher seawards. Thus, the seasonal fluctuation of the salinity ecocline had a greater power to predict larvae distribution and diversity by retaining larvae in essential habitats with suitable environmental conditions.

Use of estuarine resources by top predator fishes. How do ecological patterns affect rates of contamination by microplastics?

This study assessed the seasonal patterns of habitat utilization, feeding ecology and microplastic contamination in different ontogenetic phases of sympatric snooks (Centropomus undecimalis and C. mexicanus) inhabiting a tropical estuary. More than 50% of snooks, in all ontogenetic phases, ingested microplastics (1.5 ±â€¯0.1 and 1.4 ±â€¯0.1 particles ind-1). Juveniles migrated to nursery grounds in the upper estuary, during the early dry (C. undecimalis 6.5 ±â€¯2.8 ind-1) (p < 0.01) and early rainy seasons (C. mexicanus 4.1 ±â€¯1.9 ind-1). There, they fed mostly on invertebrates (Polychaeta) (p < 0.01), and became contaminated by microplastics (C. undecimalis: 0.8 ±â€¯0.4 particles ind-1; C. mexicanus: 1.7 ±â€¯0.5 particles ind-1). Sub-adults of both species forage principally in the estuarine habitats after shifting their diet from invertebrates (shrimps) in the upper reaches (1806.4 ±â€¯1729.6 mg ind-1) to pelagic fishes (R. bahiensis) in seaward habitats (2507.7 ±â€¯1758.4 mg ind-1). During feeding continues the contamination by microplastics (3.1 ±â€¯0.8 part. ind-1). Adults use the adjacent coastal as feeding and spawning grounds during the rainy season. In this phase, snooks are mostly piscivorous (R. bahiensis: up to 5303.8 ±â€¯3213.4 mg ind-1), but also ingest penaeid shrimp as complementary item (up to 175.9 ±â€¯156.7). Microplastics contamination rates increased towards the adult phase, with maximum contamination coinciding with peaks of fish ingestion, suggesting trophic transfer of microplastics. The lower estuary and adjacent coastal zone were important contamination sites, especially during the rainy season (up to 3.1 ±â€¯0.8 part. ind-1) (p < 0.01), when fishery activities is intense and river basin runoff increases. Consequently, the availability of microplastics is higher during this time of year in the lower portion of the estuary. Snooks had similar prey preferences, but the use of different habitats along the life cycle of each species avoids overlaps in estuarine use and minimizes competition.

Distribution, sources and consequences of nutrients, persistent organic pollutants, metals and microplastics in South American estuaries.

Estuarine pollution imposes rapid, increasing and lasting environmental modifications. In the present review, especial attention is given to estuaries in South America (SA), where legislation, policies and actions to guarantee environmental quality remain ineffective. There, the majority of estuaries face uncontrolled occupation of its margins by urban and industrial centres, agriculture and aquaculture expansion, water extraction and flow control. The lack of basic sanitation and poor environmental management (including territories within Marine Protected Areas) often lead to hydrological alterations, high nutrient loads, and the presence and dynamics of pollutants (nutrient loads, persistent organic pollutants (POPs), metals and plastic debris) along the entire estuarine ecocline. Organic enrichment has increased dissolved oxygen consumption, with wide spatio-temporal variability along latitudes and estuarine gradients. The toxicity, biogeochemistry and availability of metals and POPs depend on the annual fluctuations of salinity, water renewal, dissolved oxygen levels, suspended particulate loads, sediment mobility, grain size and composition at the sink. Plastic debris from land sources are widespread in estuaries, where they continue to fragment into microplastics. River basins are the main contributors of plastics to estuaries, whose transportation and accumulation are subjected to interannual water flow variations. Although some systems seems to be in a better condition in relation to others around the world (e.g. Goiana and Negro estuaries), many others are among the most modified worldwide (e.g. Guanabara Bay and Estero Salado System). We propose that, estuarine conservation plans should consider year-round fluctuations of the ecocline and the resulting cycles of retention and flush of environmental signals and their influence on trophic webs over the whole extent of estuarine gradients.

Dynamics of Marine Debris Ingestion by Profitable Fishes Along The Estuarine Ecocline.

The dynamics of microfilament (<5 mm) ingestion were evaluated in three species of snooks. The ingestion of different colours and sizes of microfilaments were strongly associated with the spatio-temporal estuarine use and ontogenetic shifts of snooks. Their feeding ecology was also analysed to assess dietary relationships with patterns of contamination. All species were highly contaminated with microfilaments. The highest ingestion of microfilaments occurred in the adults, when fishes became the main prey item and also during the peak of fishing activities, in the rainy season. This suggests that trophic transfer, in addition to periods of high availability of microfilaments are important pathways for contamination. The ingestion of microfilaments of different colours and sizes was likely influenced by input sources. Blue microfilaments were frequently ingested, and appear to have both riverine and estuarine inputs, since they were ingested in all seasons and habitats. Purple and red microfilaments were more frequently ingested in the lower estuarine habitats. The length of microfilaments was also associated with environmental variability. Longer microfilaments were ingested in habitats with greater riverine influence, the opposite was observed for shorter microfilaments. Therefore, microfilament contamination in snooks are a consequence of their ecological patterns of estuarine uses through different seasons and life history stages.

Use of resources and microplastic contamination throughout the life cycle of grunts (Haemulidae) in a tropical estuary.

The distribution, feeding ecology and microplastic contamination were assessed in different ontogenetic phases of Haemulidae species inhabiting the Goiana Estuary, over a seasonal cycle. Pomadasys ramosus and Haemulopsis corvinaeformis are estuarine dependent species that use habitats with specific environmental conditions each season. Pomadasys ramosus was found in the upper and middle estuaries during the rainy season, when salinity showed the lowest values. Haemulopsis corvinaeformis was found in the lower estuary during the dry season, when salinity increased in the estuary. Juveniles of P. ramosus are zooplanktivores, feeding mainly on calanoid copepods. Sub-adults and adults are zoobenthivores, feeding on invertebrates associated to the bottom, mainly Polychaeta. Juveniles of H. corvinaeformis were not found in the main channel, but sub-adults and adults showed a zoobenthivore habit, feeding mainly on Anomalocardia flexuosa (Mollusca: Bivalvia). Dietary shifts along the life cycle and the spatio-temporal relationship between their distribution and the availability of microplastics along the estuary seem to have a strong influence in the ingestion of microfilaments. The highest average ingestion of microfilaments by P. ramosus coincided with the peak of ingestion of Polychaeta by sub-adults in the upper estuary during the late rainy season. For H. corvinaeformis the highest ingestion of microfilaments coincided with the peak of ingestion of A. flexuosa by adults in the lower estuary during the late dry season. Such contamination might be attributed to the time when these phases shifted to a more diverse diet and began to forage on benthic invertebrates. Research on microplastic contamination must consider species-specific behaviour, since the intake of microplastics is dependent on patterns of distribution and trophic guild within fish assemblages.

High intake rates of microplastics in a Western Atlantic predatory fish, and insights of a direct fishery effect.

Microplastic contamination was investigated in the gut contents of an economically important estuarine top predator, Cynoscion acoupa, according to spatiotemporal and ontogenetic use of a tropical estuary. Microplastic contamination was found in more than half of the analysed fish. Ingested microplastics were classified by type, colour and length with most of the particles consisting of filaments (<5 mm). Longer filaments were more frequently ingested in the upper estuary and smaller filaments in the lower estuary, as a result of differences in hydrodynamic forces and proximity to the probable input sources. The river is likely an important source of filaments to the estuary and filaments ingested in the upper estuary showed little sign of weathering, when compared with those from the lower estuary, which are subject to intense weathering and consequent break-up of particles to smaller sizes. Most filaments, of all colours, accumulated in adults of C. acoupa, which are more susceptible to contamination through both direct ingestion and trophic transference as they shift their feeding mode to piscivory. Moreover, the highest ingestion of filaments in adults occurred in the lower estuary, during the late rainy season, likely associated with the intense fishing activities in this habitat, which results in a greater input of filaments from fishing gear, which are mainly blue in colour. Overall, 44% of the ingested filaments were blue, 20% purple, 13% black, 10% red and 12% white. The next most common colour, the purple filaments, are most likely blue filaments whose colour has weathered to purple. Red filaments were proportionally more ingested in the lower estuary, indicating a coastal/oceanic source. White and black filaments were more commonly ingested in the inner estuary, suggesting that they have a riverine origin and/or were actively ingested by juveniles and sub-adults, which inhabit the inner estuary and have zooplankton as an important food resource.