Mechanistic insights into the interactions of TAX1BP1 with RB1CC1 and mammalian ATG8 family proteins.

TAX1BP1, a multifunctional autophagy adaptor, plays critical roles in different autophagy processes. As an autophagy receptor, TAX1BP1 can interact with RB1CC1, NAP1, and mammalian ATG8 family proteins to drive selective autophagy for relevant substrates. However, the mechanistic bases underpinning the specific interactions of TAX1BP1 with RB1CC1 and mammalian ATG8 family proteins remain elusive. Here, we find that there are two distinct binding sites between TAX1BP1 and RB1CC1. In addition to the previously reported TAX1BP1 SKICH (skeletal muscle and kidney enriched inositol phosphatase (SKIP) carboxyl homology)/RB1CC1 coiled-coil interaction, the first coiled-coil domain of TAX1BP1 can directly bind to the extreme C-terminal coiled-coil and Claw region of RB1CC1. We determine the crystal structure of the TAX1BP1 SKICH/RB1CC1 coiled-coil complex and unravel the detailed binding mechanism of TAX1BP1 SKICH with RB1CC1. Moreover, we demonstrate that RB1CC1 and NAP1 are competitive in binding to the TAX1BP1 SKICH domain, but the presence of NAP1's FIP200-interacting region (FIR) motif can stabilize the ternary TAX1BP1/NAP1/RB1CC1 complex formation. Finally, we elucidate the molecular mechanism governing the selective interactions of TAX1BP1 with ATG8 family members by solving the structure of GABARAP in complex with the non-canonical LIR (LC3-interacting region) motif of TAX1BP1, which unveils a unique binding mode between LIR and ATG8 family protein. Collectively, our findings provide mechanistic insights into the interactions of TAX1BP1 with RB1CC1 and mammalian ATG8 family proteins and are valuable for further understanding the working mode and function of TAX1BP1 in autophagy.

Interfacial Charge Transport in 1D TiO2 Based Photoelectrodes for Photoelectrochemical Water Splitting.

1D nanostructured photoelectrodes are promising for application as photoelectrochemical (PEC) devices for solar energy conversion into hydrogen (H2 ) owing to the optical, structural, and electronic advantages. Titanium dioxide (TiO2 ) is the most investigated candidate as a photoelectrode due to its good photostability, low production cost, and eco-friendliness. The obstacle for TiO2 's practical application is the inherent wide bandgap (UV-lights response), poor conductivity, and limited hole diffusion length. Here, a comprehensive review of the current research efforts toward the development of 1D TiO2 based photoelectrodes for heterogeneous PEC water splitting is provided along with a discussion of nanoarchitectures and energy band engineering influences on interfacial charge transfer and separation of 1D TiO2 composited with different dimensional photoactive materials. The key focus of this review is to understand the charge transfer processes at interfaces and the relationship between photogenerated charge separation and photoelectrochemical performance. It is anticipated that this review will afford enriched information on the rational designs of nanoarchitectures, doping, and heterojunction interfaces for 1D TiO2 based photoelectrodes to achieve highly efficient solar energy conversion.

Surface electromyographic characteristics of forearm muscles after ulnar and radius fracture inchildren.

Objective: To evaluate the characteristics of forearm muscle activity in children with ulnar and radius fractures during different follow-up periods by surface electromyography. Methods: A retrospective analysis was performed on 20 children with ulnar and radius fractures treated with an elastic intramedullary nail from October 2020 to December 2021. All children were treated with transcubital casts after surgery. At 2 months and before taking out the elastic intramedullary nail, surface electromyographic signals were collected on the flexor/extension of the wrist and the maximum arbitrary isometric contraction of the grip strength in the forearm flexor and extensor muscles of the forearm. The root-mean-square values and integrated EMG values of the superficial flexor and extensor digitalis of the healthy side and the affected side were collected at the last follow-up and 2 months after surgery, and the co-systolic ratio was calculated. The root-mean-square values and co-systolic ratio were compared and analyzed, and the Mayo wrist function score was evaluated. Results: The mean follow-up time was (8.4 ± 2.85) months. Mayo scores were (87.42 ± 13.01) and (97.69 ± 4.50) points at the last follow-up and two months after surgery, respectively (p < 0.05). In the test of grip strength, 2 months after surgery, the grip strength of the affected side was lower than that of the healthy side (p < 0.05), and the maximum and mean values of the superficial flexor of the affected side were lower than those of the healthy side (p < 0.05). At the last follow-up, there was no difference in the grip strength between the affected side and the healthy side (p > 0.05), and no difference in the maximum RMS, mean RMS and cooperative contraction ratio of the superficial flexor and digital extensor muscles between the affected side and the healthy side (p > 0.05). Conclusion: Satisfactory results can be obtained after elastic intramedullary napping in children with ulnar and radius fractures. However, 2 months after surgery, the grip strength of the affected side is small, and the electrical activity of the forearm muscle is low during flexion and extension activities of the wrist joint, which has not returned to normal, suggesting that children orthopaedic clinicians should remind children to conduct timely and effective rehabilitation training after the removal of the cast.

Mechanistic insights into the enzymatic activity of E3 ligase HOIL-1L and its regulation by the linear ubiquitin chain binding.

Heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1L) serves as a unique E3 ligase to catalyze the mono-ubiquitination of relevant protein or sugar substrates and plays vital roles in numerous cellular processes in mammals. However, the molecular mechanism underpinning the E3 activity of HOIL-1L and the related regulatory mechanism remain elusive. Here, we report the crystal structure of the catalytic core region of HOIL-1L and unveil the key catalytic triad residues of HOIL-1L. Moreover, we discover that HOIL-1L contains two distinct linear di-ubiquitin binding sites that can synergistically bind to linear tetra-ubiquitin, and the binding of HOIL-1L with linear tetra-ubiquitin can promote its E3 activity. The determined HOIL-1L/linear tetra-ubiquitin complex structure not only elucidates the detailed binding mechanism of HOIL-1L with linear tetra-ubiquitin but also uncovers a unique allosteric ubiquitin-binding site for the activation of HOIL-1L. In all, our findings provide mechanistic insights into the E3 activity of HOIL-1L and its regulation by the linear ubiquitin chain binding.

Design of the Physical Fitness Evaluation Information Management System of Sports Athletes Based on Artificial Intelligence.

With the rapid development of science and technology in recent years, more and more researchers began to explore the basic disciplines of sports, namely, biomechanics and physiology, and further update and improve the traditional sports training theory. However, the developed countries, i.e., Europe and the United States, have dedicatedly worked on the effective role of physical training particularly in middle-class farmers in sports powers. They have realized that the continuous improvement of the physical training evaluation system is directly related to the reforming in the evaluation and monitoring methods of physical training. At the same time, there is a demand for athletes' physical fitness evaluation, which requires a large amount of data support during the evaluation. These data collection and analysis are difficult and consume a lot of manpower. Therefore, this paper uses artificial intelligence technology to design the sports athletes' physical fitness evaluation information management system to improve the comprehensiveness of system data collection and the accuracy of data analysis. Through the physical attenuation calculation and physical analysis of the system developed in this paper, data accuracy is high. The coach can make a training plan according to the physical consumption of athletes in sports training or competition, which can greatly improve the ability of athletes.

PavGA2ox-2L inhibits the plant growth and development interacting with PavDWARF in sweet cherry (Prunus avium L.).

Dwarf dense planting is helpful to improve the yield and quality of sweet cherry, which has enormous market demand. GA2oxs (GA oxidases) affect plant height, dormancy release, flower development, and seed germination by participating in the metabolic regulation and signal transduction of GA (Gibberellin). However, the research on GA2ox in sweet cherry is little and worthy of further investigation. Therefore, we identified the PavGA2ox-2L gene from sweet cherry, close to PynGA2ox-2 from Prunus yedoensis var. Nudiflora. The phylogenetic analysis indicated conserved functions with these evolutionarily closer GA2ox subfamily genes. Subcellular localization forecast analysis indicated that PavGA2ox-2L was localized in the nucleus or cytoplasm. The expression levels of PavGA2ox-2L were higher in winter, indicating that PavGA2ox-2L promoted maintained flower bud dormancy. The expression levels of PavGA2ox-2L were significantly increased after GA4+7 treatment while decreased after GR24 (a synthetic analog of SLs (Strigolactones)) or TIS108 (a triazole-type SL-biosynthesis inhibitor) treatments. Over-expression of PavGA2ox-2L resulted in decreased plant height, delayed flowering time, and low seed germination rate in Arabidopsis thaliana. Furthermore, the interaction between PavGA2ox-2L and PavDWARF was verified by Y2H and BiFC assays. In the current investigation, PavGA2ox-2L functions as a GA metabolic gene that promotes dwarf dense planting, delays flowering time, and inhibits seed germination. In addition, it also participates in regulating plant growth and development through the interaction with the critical negative regulator PavDWARF of Gibberellin. These results will help us better explore the molecular mechanism of GA2ox-mediated dwarf and late-maturing varieties for fruit trees.

Carbon-Coating Layers on Boron Generated High Critical Current Density in MgB2 Superconductor.

Boron particles with a homogeneous carbon-coating layer were employed as the precursor to fabricate MgB2 superconductors to generate artificial two-dimensional (2D) flux-pinning centers. Systematic microstructure investigation reveals that the carbon layers are well-distributed in the MgB2 matrix without agglomeration. The thickness of the carbon layers is smaller than the MgB2 coherent length, which makes them transparent to supercurrent. The critical current density is increased because of the strong flux-pinning effects of the 2D carbon layers in the superconductor as highly efficient flux-pinning centers and the increased irreversibility field due to the carbon-doping effects.