Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Filtrar
1.
J Clin Invest ; 102(2): 379-85, 1998 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-9664079

RESUMO

HDL metabolism and atherosclerosis were studied in apo E knockout (KO) mice overexpressing human apo AI, a des- (190-243)-apo AI carboxyl-terminal deletion mutant of human apo AI or an apo AI-(1-189)-apo AII-(12-77) chimera in which the carboxyl-terminal domain of apo AI was substituted with the pair of helices of apo AII. HDL cholesterol levels ranked: apo AI/apo E KO approximately apo AI-(1-189)-apo AII- (12-77)/apo E KO > > des-(190-243)-apo AI/apo E KO > apo E KO mice. Progression of atherosclerosis ranked: apo E KO > des-(190-243)-apo AI/apo E KO > > apo AI-(1-189)- apo AII-(12-77)/apo E KO approximately apo AI/apo E KO mice. Whereas the total capacity to induce cholesterol efflux from lipid-loaded THP-1 macrophages was higher for HDL of mice overexpressing human apo AI or the apo AI/apo AII chimera, the fractional cholesterol efflux rate, expressed in percent cholesterol efflux/microg apolipoprotein/h, for HDL of these mice was similar to that for HDL of mice overexpressing the deletion mutant and for HDL of apo E KO mice. This study demonstrates that the tertiary structure of apo AI, e.g., the number and organization of its helices, and not its amino sequence is essential for protection against atherosclerosis because it determines HDL cholesterol levels and not cholesterol efflux. Amino acid sequences of apo AII, which is considered to be less antiatherogenic, can be used to restore the structure of apo AI and thereby its antiatherogenicity.


Assuntos
Apolipoproteína A-II/metabolismo , Apolipoproteína A-I/metabolismo , Apolipoproteínas E/fisiologia , Arteriosclerose/metabolismo , HDL-Colesterol/metabolismo , Substituição de Aminoácidos , Animais , Apolipoproteína A-I/genética , Apolipoproteína A-II/genética , Apolipoproteínas E/genética , Arteriosclerose/patologia , Sítios de Ligação , Ácidos Carboxílicos , Linhagem Celular , Colesterol/metabolismo , Progressão da Doença , Feminino , Genótipo , Humanos , Lipoproteínas/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout
2.
Circulation ; 103(20): 2495-500, 2001 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-11369691

RESUMO

BACKGROUND: Atherosclerosis is characterized by an early inflammatory response involving proinflammatory mediators such as platelet-activating factor (PAF)-like phospholipids, which are inactivated by PAF-acetylhydrolase (PAF-AH). The effect of adenovirus-mediated expression of PAF-AH on injury-induced neointima formation and spontaneous atherosclerosis was studied in apolipoprotein E-deficient mice. METHODS AND RESULTS: Intravenous administration of an adenovirus (5 x 10(8) plaque-forming units) directing liver-specific expression of human PAF-AH resulted in a 3.5-fold increase of plasma PAF-AH activity at day 7 (P<0.001); this was associated with a 2.4- and 2.3-fold decrease in malondialdehyde-modified LDL autoantibodies and the lysophosphatidylcholine/phosphatidylcholine ratio, respectively (P<0.001 for both). Non-HDL and HDL cholesterol levels in PAF-AH-treated mice were similar to those of control virus-treated mice. Seven days after virus injection, endothelial denudation of the common left carotid artery was induced with a guidewire. Neointima formation was assessed 18 days later. PAF-AH gene transfer reduced oxidized lipoproteins by 82% (P<0.001), macrophages by 69% (P=0.006), and smooth muscle cells by 84% (P=0.002) in the arterial wall. This resulted in a 77% reduction (P<0.001) of neointimal area. Six weeks after adenovirus-mediated gene transfer, spontaneous atherosclerotic lesions in the aortic root were analyzed. PAF-AH gene transfer reduced atherosclerotic lesions by 42% (P=0.02) in male mice, whereas a nonsignificant 14% reduction was observed in female mice. Basal and PAF-AH activity after gene transfer were higher in male mice than in female mice (P=0.01 and P=0.04, respectively). CONCLUSIONS: Gene transfer of PAF-AH inhibited injury-induced neointima formation and spontaneous atherosclerosis in apolipoprotein E-deficient mice. Our data indicate that PAF-AH, by reducing oxidized lipoprotein accumulation, is a potent protective enzyme against atherosclerosis.


Assuntos
Adenoviridae/genética , Apolipoproteínas E/deficiência , Arteriosclerose/prevenção & controle , Fosfolipases A/genética , Túnica Íntima/patologia , 1-Alquil-2-acetilglicerofosfocolina Esterase , Animais , Apolipoproteínas E/genética , Arteriosclerose/genética , HDL-Colesterol/sangue , VLDL-Colesterol/sangue , Feminino , Expressão Gênica , Técnicas de Transferência de Genes , Vetores Genéticos/genética , Humanos , Fígado/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Estresse Oxidativo/genética , Fosfolipases A/sangue , RNA/genética , RNA/metabolismo , Fatores de Tempo , Túnica Íntima/metabolismo
3.
Arterioscler Thromb Vasc Biol ; 20(10): E68-75, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11031226

RESUMO

Various mechanisms may contribute to the antiatherogenic potential of apolipoprotein A-I (apo A-I) and high density lipoproteins (HDLs). Therefore, the effect of adenovirus-mediated human apo A-I gene transfer or human apo A-I transgenesis on platelet-activating factor acetylhydrolase (PAF-AH) and arylesterase/paraoxonase (PON1) was studied in C57BL/6 and C57BL/6 apo E(-/-) mice. Human apo A-I transgenesis in C57BL/6 mice resulted in a 4.2-fold (P<0.0001) increase of PAF-AH and a 1.7-fold (P=0.0012) increase of PON1 activity. The apo E deficiency was associated with a 1.6-fold (P=0.008) lower PAF-AH and a 2.0-fold (P=0.012) lower PON1 activity. Human apo A-I transgenesis in C57BL/6 apo E(-/-)mice increased PAF-AH and PON1 activity by 2.1-fold (P=0.01) and 2.5-fold (P=0.029), respectively. After adenovirus-mediated gene transfer of human apo A-I into C57BL/6 apo E(-/-)mice, a strong correlation between human apo A-I plasma levels and PAF-AH activity was observed at day 6 (r=0.92, P<0.0001). However, PON1 activity failed to increase, probably as a result of cytokine-mediated inhibition of PON 1 expression. In conclusion, this study indicates that overexpression of human apo A-I increases HDL-associated PAF-AH activity. PON1 activity was also increased in human apo A-I transgenic mice, but not after human apo A-I gene transfer, a result that was probably related to cytokine production induced in the liver by the adenoviral vectors. Increased levels of these HDL-associated enzymes may contribute to the anti-inflammatory and antioxidative potential of HDL and thereby to the protection conferred by HDL against atherothrombosis.


Assuntos
Apolipoproteína A-I/genética , Apolipoproteínas E/deficiência , Lipoproteínas HDL/sangue , 1-Alquil-2-acetilglicerofosfocolina Esterase , Adenoviridae/genética , Animais , Antioxidantes/metabolismo , Apolipoproteína A-I/biossíntese , Apolipoproteína A-I/sangue , Arildialquilfosfatase , Eletroforese das Proteínas Sanguíneas , HDL-Colesterol/sangue , Cromatografia em Gel , Complemento C3/análise , Citocinas/sangue , Esterases/genética , Esterases/metabolismo , Feminino , Regulação da Expressão Gênica , Técnicas de Transferência de Genes , Humanos , Lipoproteínas HDL/química , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Fosfolipases A/genética , Fosfolipases A/metabolismo , Fator de Ativação de Plaquetas/metabolismo , Albumina Sérica/análise , Fatores de Tempo , Regulação para Cima , alfa-Macroglobulinas/análise
4.
Arterioscler Thromb Vasc Biol ; 21(12): 1977-83, 2001 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11742873

RESUMO

Atherosclerosis was studied in apolipoprotein E (apoE) knockout mice expressing human apolipoprotein A-I (apoA-I) or an apoA-I/apolipoprotein A-II (apoA-II) chimera in which the Arg123-Tyr166 central domain of apoA-I was substituted with the Ser12-Ala75 segment of apoA-II. High density lipoprotein (HDL) cholesterol levels were identical in apoA-I and apoA-I/apoA-II mice, but at 4 months, plaques were 2.7-fold larger in the aortic root of the apoA-I/apoA-II mice (P<0.01). The macrophage-to-smooth muscle cell ratio of lesions was 2.1-fold higher in apo-I/apoA-II mice than in apoA-I mice (P<0.01). This was due to a 2.7-fold higher (P<0.001) in vivo macrophage homing in the aortic root of apoA-I/apoA-II mice. Plasma platelet-activating factor acetyl hydrolase activity was lower (P<0.01) in apoA-I/apoA-II mice, resulting in increased oxidative stress, as evidenced by the higher titer of antibodies against oxidized low density lipoprotein (P<0.01). Increased oxidative stress resulted in increased stimulation of ex vivo macrophage adhesion by apoA-I/apoA-II beta-very low density lipoprotein and decreased inhibition of beta-very low density lipoprotein-induced adhesion by HDL from apoA-I/apoA-II mice. The cellular cholesterol efflux capacity of HDL from apoA-I/apoA-II mice was very similar to that of apoA-I mice. Thus, the Arg123-Tyr166 central domain of apoA-I is critical for reducing oxidative stress, macrophage homing, and early atherosclerosis in apoE knockout mice independent of its role in HDL production and cholesterol efflux.


Assuntos
Apolipoproteína A-I/genética , Arteriosclerose/fisiopatologia , HDL-Colesterol/metabolismo , Macrófagos/metabolismo , Animais , Autoanticorpos/análise , Sequência de Bases , Adesão Celular , Quimera , Progressão da Doença , Feminino , Lipoproteínas HDL/sangue , Lipoproteínas LDL/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Transgênicos , Estresse Oxidativo/genética
5.
Hum Gene Ther ; 11(1): 101-12, 2000 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-10646643

RESUMO

Elevation of HDL cholesterol, after adenoviral apolipoprotein A-I (apo A-I) gene transfer, may delay or revert ischemic cardiovascular disease, provided transgene expression is persistent. Previously, we observed transient human apo A-I expression after adenoviral gene transfer with a cytomegalovirus (CMV)-driven construct containing the human apo A-I cDNA. Therefore, the effects of promoters (CMV or 256 base pairs of the human apo A-I promoter), introns of the human apo A-I gene, and the liver-specific human apolipoprotein E (apo E) enhancer on adenovirus-mediated human apo A-I expression were evaluated in C57BL/6 mice. In the presence of the CMV promoter, human apo A-I introns prolonged expression above 20 mg/dl from 14 to 35 days. Addition of one, two, or four copies of the human apo E enhancer in these constructs resulted in a copy-dependent but transient increase in expression for 14 days. The apo A-I promoter induced 3.2-fold lower peak levels of human apo A-I than did the CMV promoter, but insertion of four apo E enhancers in the apo A-I promoter-driven construct resulted in human apo A-I levels above 20 mg/dl for 6 months. The decline between day 6 and day 35 of human apo A-I expression driven by the CMV promoter was due to (1) a 2.5-fold decline in transgene DNA levels that is not observed with apo A-I promoter-driven constructs, and (2) CMV promoter attenuation as evidenced by a 7.6-fold decline in the human apo A-I mRNA/human apo A-I DNA copy number ratio between day 6 and day 35. Hepatotoxicity, as evidenced by up to 10-fold higher serum levels of transaminases on day 6 after gene transfer with CMV promoter-driven constructs than with apo A-I promoter-driven constructs, probably caused the accelerated decline of transgene DNA. In conclusion, gene transfer with an adenovirus comprising the 256-bp apo A-I promoter, the genomic apo A-I DNA, and four apo E enhancers, all of human origin, is associated with low hepatotoxicity and with the absence of promoter shutoff resulting in human apo A-I expression above 20 mg/dl for up to 6 months.


Assuntos
Adenoviridae/genética , Apolipoproteína A-I/genética , Apolipoproteínas E/genética , Técnicas de Transferência de Genes , Animais , Sequência de Bases , Divisão Celular/genética , Primers do DNA , Elementos Facilitadores Genéticos , Feminino , Vetores Genéticos/efeitos adversos , Humanos , Íntrons , Fígado/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas , Recombinação Genética , Linfócitos T/citologia
6.
Gynecol Oncol ; 49(2): 243-6, 1993 May.
Artigo em Inglês | MEDLINE | ID: mdl-8389313

RESUMO

We sought to determine the prevalence of human immunodeficiency virus (HIV) infection in a population of women with human papillomavirus (HPV)-related diseases attending a colposcopy clinic who had no other CDC-defined risk factors for HIV. Study patients included all new patients attending our colposcopy clinic who were found to have histologic evidence of condyloma or cervical intraepithelial neoplasia. Those patients not already known to be HIV-positive were offered testing for HIV. Demographic information was obtained on all patients. Results were compared to data from anonymous testing of our own obstetrical population. One hundred forty of 208 women (67.3%) were either previously known to be HIV-positive or agreed to be tested. Sixteen (11.4%) were HIV-positive. Eight of the HIV-positive women were not previously known to be HIV-positive and 6 of the 8 had no definable risk factors for HIV infection. This is 4.6% of the women not already known to have a CDC-defined risk factor for HIV. The rate of HIV infection in our obstetrical population is 1.6%. In women without other definable risks for HIV infection and who had HPV-related disease the relative risk of HIV infection in our population was 2.94 (95% confidence interval 1.21-6.94; P < 0.031). In areas where HIV is endemic there is a high prevalence of HIV infection in women with HPV-related disease. Even in women without another definable risk factor for HIV, HPV-related disease may serve as a marker for an increased risk of HIV infection in this population.


Assuntos
Infecções por HIV/etiologia , Papillomaviridae/patogenicidade , Infecções Tumorais por Vírus/complicações , Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Fatores de Risco
7.
Arterioscler Thromb Vasc Biol ; 20(2): 459-66, 2000 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10669644

RESUMO

High density lipoprotein (HDL) metabolism and lecithin:cholesterol acyltransferase (LCAT)-induced HDL remodeling were investigated in transgenic mice expressing human apolipoprotein (apo) AI or an apoAI/apoAII chimera in which the Arg123-Tyr166 domain of apoAI was substituted with the Ser12-Ala75 domain of apoAII. Expression of apoAI and of the apoAI/apoAII chimera resulted in a respective 3. 5-fold and 2.9-fold increase of HDL cholesterol. Human LCAT gene transfer into apoAI-transgenic mice resulted in a 5.1-fold increase of endogenous LCAT activity. This increase was associated with a 2. 4-fold increase of the cholesterol ester-to-free cholesterol ratio of HDL, a shift from HDL(3) to HDL(2), and a 2.4-fold increase of HDL cholesterol levels. Agarose gel electrophoresis revealed that human LCAT gene transfer into human apoAI-transgenic mice resulted in an increase of pre-beta-HDL and of pre-alpha-HDL. In contrast, human LCAT gene transfer did not affect cholesterol levels and HDL distribution profile in mice expressing the apoAI/apoAII chimera. Mouse LCAT did not "see" a difference between wild-type and mutant human apoAI, whereas human LCAT did, thus localizing the species-specific interaction in the central domain of apoAI. In conclusion, the Arg123-Tyr166 central domain of apoAI is not critical for in vivo lipoprotein association. It is, however, critical for LCAT-induced hyperalphalipoproteinemia and HDL remodeling independent of the lipid-binding properties of apoAI.


Assuntos
Apolipoproteína A-I/genética , Hiperlipoproteinemias/induzido quimicamente , Hiperlipoproteinemias/genética , Lipoproteínas HDL/sangue , Fosfatidilcolina-Esterol O-Aciltransferase , Sequência de Aminoácidos/genética , Animais , Apolipoproteína A-I/sangue , Apolipoproteínas/sangue , Quimera , HDL-Colesterol/sangue , Técnicas de Transferência de Genes , Genótipo , Humanos , Lipoproteínas/sangue , Camundongos , Camundongos Transgênicos/genética , Fosfatidilcolina-Esterol O-Aciltransferase/sangue , Fosfatidilcolina-Esterol O-Aciltransferase/genética
8.
FASEB J ; 14(13): 2032-9, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11023987

RESUMO

Macrophage infiltration into the subendothelial space at lesion prone sites is the primary event in atherogenesis. Inhibition of macrophage homing might therefore prevent atherosclerosis. Since HDL levels are inversely correlated with cardiovascular risk, their effect on macrophage homing was assessed in apoE-deficient (apoE-/-) mice. Overexpression of human apolipoprotein AI in apoE-/- mice increased HDL levels 3-fold and reduced macrophage accumulation in an established assay of leukocyte homing to aortic root endothelium 3.2-fold (P<0.005). This was due to reduced in vivo betaVLDL oxidation, reduced betaVLDL triggered endothelial cytosolic Ca2+ signaling through PAF-like bioactivity, lower ICAM-1 and VCAM-1 expression, and diminished ex vivo leukocyte adhesion. Adenoviral gene transfer of human PAF-acetylhydrolase (PAF-AH) in apoE-/- mice increased PAF-AH activity 1.5-fold (P<0.001), reduced betaVLDL-induced ex vivo macrophage adhesion 3.5-fold (P<0.01), and reduced in vivo macrophage homing 2.6-fold (P<0.02). These inhibitory effects were observed in the absence of increased HDL cholesterol levels. In conclusion, HDL reduces macrophage homing to endothelium by reducing oxidative stress via its associated PAF-AH activity. This protective mechanism is independent of the function of HDL as cholesterol acceptor. Modulation of lipoprotein oxidation by PAF-AH may prevent leukocyte recruitment to the vessel wall, a key feature in atherogenesis.


Assuntos
Apolipoproteínas E/genética , Endotélio Vascular/fisiologia , Leucócitos/fisiologia , Lipoproteínas HDL/metabolismo , Macrófagos Peritoneais/fisiologia , Fosfolipases A/metabolismo , 1-Alquil-2-acetilglicerofosfocolina Esterase , Animais , Apolipoproteína A-I/biossíntese , Arteriosclerose , Sinalização do Cálcio , Adesão Celular , Colesterol/sangue , Citosol/metabolismo , Humanos , Lipoproteínas VLDL/metabolismo , Camundongos , Camundongos Mutantes , Modelos Biológicos , Estresse Oxidativo/fisiologia , Fosfolipases A/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA