RESUMO
OBJECTIVE: To investigate the expression of bcl-2 and bax genes in the liver cancer cell line HepG2 after apoptosis induced by essential oils from Rosmarinus officinalis. METHODS: Essential oils from Rosmarinus officinalis were collected by steam distillation, then its chemical composition was determined by GC-MS. Expression of bcl-2 and bax genes were detected by immunohistochemical method. RESULTS: The liver cancer cell line HepG2 was treated with essential oils from Rosmarinus officinalis at various concentrations for different duration. Expression of bcl-2 gene reduced and expression of bax gene increased in a dose and time dependent manner. CONCLUSION: Essential oils from Rosmarinus officinalis can affect the pattern of bcl-2 and bax genes expressions, and this may increase the apoptosis of liver cancer cell line HepG2.
Assuntos
Apoptose/efeitos dos fármacos , Óleos Voláteis/farmacologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Rosmarinus/química , Proteína X Associada a bcl-2/metabolismo , Antineoplásicos/farmacologia , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Imuno-Histoquímica , Neoplasias Hepáticas/patologia , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Folhas de Planta/química , Fatores de TempoRESUMO
To help geneticists look up genetics journals more quickly and exactly,this paper developed this search engine of genetics journals. The paper exploited the search engine with genetics journals sources of Medline database in NCBI, indexed and listed 173 names of genetics journals from Medline. Then obtained one search engine concerning genetics journals after making every journal's super linkage. The search engine can search out not only all articles' titles of 173 genetics journals but also some articles' titles of the journals each year quickly, exactly and dynamically. We can further obtain all articles' abstracts and some full texts in PubMed.