RESUMO
Hydrogen sulfide (H2S), as an important small molecule bioregulator, plays a key role in many physiological activities and signaling, and abnormal fluctuations in H2S concentration can lead to a variety of diseases. Therefore, it is of great significance to develop a near-infrared fluorescence probe to visualize fluctuations in H2S levels. This work is based on Sulfur-substituted dicyanomethylene-4 H-chromene (DCM), A novel NIR fluorescent probe (E) -3 - (2 - (4 - (dicyanomethylene) -6-methyl-4 H-Thiochromen-2-yl)vinyl-1-methylquinolin-1-ium (DMT) was synthesized successfully. Research has found that in weakly alkaline environments, the probe DMT reacts rapidly with H2S (only 10 s), the fluorescence intensity at 684 nm is enhanced by about 60 fold, the detection limit is as low as 0.1623 µM, the Stokes shift is large (94 nm), and strong selectivity as well as anti-interference ability towards H2S. This will provide a new method for the rapid detection and further application of H2S.
RESUMO
Bisulfite (HSO3-) and biological thiols molecules, such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), play important roles in organisms. Developing a fluorescent probe that can simultaneously detect and distinguish HSO3- and biological thiols is of great significance. In this study, ethyl(2E,4Z)-5-chloro-2-cyano-5-(7-(diethylamino)-2-oxo-2 H-chromen-3-yl)penta-2,4-dienoate (CCO) as a novel enhanced fluorescence probe was synthesized by integrating coumarin derivatives and ethyl cyanoacetate, which can simultaneous detection and discrimination of hydrogen bisulfite anions and glutathione. The sensing mechanism was elucidated through spectral analysis and some control experiments. In weakly alkaline environments, the probe not only has good selectivity for HSO3- and GSH, but also has a lower detection limits of 0.0179 µM and 0.2034 µM. The probe exhibited fuorescent turn-on for distinguishing with 296 and 28 fold the fluorescent intensity increase at 486 and 505 nm, respectively, through diferent excitation wavelengths. This provides a new method for simultaneous detection and discrimination of HSO3- and biological thiol cell levels and further applications.