RESUMO
MicroRNAs (miRNAs) are small noncoding RNAs that play a vital role in plant responses to abiotic and biotic stresses. Recently, it has been discovered that some primary miRNAs (pri-miRNAs) encode regulatory short peptides called miPEPs. However, the presence of miPEPs in rice, and their functions in response to abiotic stresses, particularly stress induced by heavy metals, remain poorly understood. Here, we identified a functional small peptide (miPEP156e) encoded by pri-miR156e that regulates the expression of miR156 and its target SPL genes, thereby affecting miR156-mediated cadmium (Cd) tolerance in rice. Overexpression of miPEP156e led to decreased uptake and accumulation of Cd and reactive oxygen species (ROS) levels in plants under Cd stress, resulting in improved rice Cd tolerance, as observed in miR156-overexpressing lines. Conversely, miPEP156e mutants displayed sensitivity to Cd stress due to the elevated accumulation of Cd and ROS. Transcriptome analysis further revealed that miPEP156e improved rice Cd tolerance by modulating Cd transporter genes and ROS scavenging genes. Our study provides insights into the regulatory mechanism of miPEP156e in rice response to Cd stress and demonstrates the potential of miPEPs as an effective tool for improving crop abiotic stress tolerance.
Assuntos
MicroRNAs , Oryza , MicroRNAs/genética , MicroRNAs/metabolismo , Cádmio/metabolismo , Oryza/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Peptídeos/metabolismo , Regulação da Expressão Gênica de PlantasRESUMO
Promoting green technology innovation through environmental tax is an important tool to achieve green development. Based on the data of Chinese listed companies from 2010 to 2020, this research examines the impact mechanisms of environmental tax policies on the quality and quantity of enterprise green technology innovation from the micro-enterprise perspective. The underlying mechanisms and heterogeneous effects were analyzed empirically using pooled OLS model and the mediated effects model. The results indicate that the environmental tax policy has an inhibitory effect on the quantity and quality of green patents, and the impact on the quantity is more significant. The mechanism analysis suggests that the environmental tax inhibits green technology innovation via accelerating capital renewal and environmental investment. Heterogeneity analysis shows that environmental tax has an inhibitory effect on green technology innovation behavior of large-scale enterprises and eastern region enterprises, while it has a boosting effect on green technology innovation of western region enterprises, and its effect on quantity of innovation is greater than that on quality. This study helps Chinese enterprises to better achieve green development from the perspective of green taxation and provides important empirical basis for achieving the win-win goal of economic growth and environmental development.
Assuntos
Conservação dos Recursos Naturais , China , Desenvolvimento Econômico , Política Ambiental , TecnologiaRESUMO
To investigate the effect of Mn and other metal dopants on the photoelectronic performance of CsPbCl3 perovskites, we conducted a series of theoretical analyses. Our findings showed that after Mn mono-doping, the CsPbCl3 lattice contracted and the bonding strength increased, resulting in a more compact structure of the metal octahedral cage. The relaxation of the metal octahedral cage, along with the Jahn-Teller effect, results in a decrease in lattice strain between the octahedra and a reduction in the energy of the entire lattice due to the deformation of the metal octahedron. These three factors work together to reduce intrinsic defects and enhance the stability and electronic properties of CsPbCl3 perovskites. The solubility of the Mn dopant is significantly increased when co-doped with Ni, Fe, and Co dopants, as it compensates for the lattice strain induced by Mn. Doping CsPbCl3 perovskites reduces the band gap due to the decreased contributions of 3d orbitals from the dopants. Our analyses have revealed that strengthening the CsPbCl3 lattice and reducing intrinsic defects can result in improved stability and PL properties. Moreover, increasing Mn solubility and decreasing the bandgap can enhance the PLQY of orange luminescence in CsPbCl3 perovskites. These findings offer valuable insights for the development of effective strategies to enhance the photoelectronic properties of these materials.