Rickettsia Infection Benefits Its Whitefly Hosts by Manipulating Their Nutrition and Defense.

Endosymbionts play an essential role in the biology, physiology and immunity of insects. Many insects, including the whitefly Bemisia tabaci, are infected with the facultative endosymbiont Rickettsia. However, the mutualism between Rickettsia and its whitefly host remains unclear. This study investigated the biological and physiological benefits of Rickettsia infection to B. tabaci. Results revealed that infection of Rickettsia increased the fertility, the survival rate from nymph to adult and the number of female whiteflies. In addition, this facilitation caused a significant reduction in nymphal developmental duration but did not affect percentage rate of egg hatching. Rickettsia infected B. tabaci had significantly higher glycogen, soluble sugar and trehalose contents than Rickettsia negative B. tabaci individuals. Rickettsia also improved the immunity of its whitefly hosts. Rickettsia infested B. tabaci had lower mortality rates and higher semi-lethal concentrations (LC50) when exposed to the fungus Akanthomyces attenuatus and the insecticides imidacloprid and spirotetramat. The percentage of parasitism by Encarsia formosa was also reduced by Rickettsia infection. Overall, Rickettsia infection benefits B. tabaci by improving the nutritional composition of its host, and also protects B. tabaci by enhancing its resistance towards insecticides (imidacloprid and spirotetramat), entomopathogenic fungi (A. attenuatus) and its main parasitoid (E. formosa); all of which could significantly impact on current management strategies.

Effects of time after last herbivory on the attraction of corn plants infested with common arymworms to a parasitic wasp Cotesia kariyai.

Females of the gregarious endoparasitoid Cotesia kariyai were attracted to a blend of volatiles released from corn plants infested with larvae of their host, the common armyworm (Mythimna separata). We investigated the effects of time after the last infestation (1-168 h) on the attractiveness of corn plants infested by host larvae by using a wind tunnel under laboratory conditions. Immediately after the removal of the larvae, parasitoids were attracted more to plants that had been infested with the larvae than to intact plants (control). This attractiveness gradually decreased with time after the last infestation. The attractiveness of the infested plants was significantly higher than that of intact plants when the time after the last infestation was within 1 day. Fifteen herbivore-induced volatiles were recorded in the headspace of infested corn plants irrespective of time. The amounts of some compounds including (Z)-3-hexen-1-yl acetate, which have already been reported to attract C. kariyai, correlated with the attractiveness. The ecological meaning of the duration of production of C. kariyai attractants is discussed.

[Effectiveness of Eretmocerus sp. and Aschersonia aleyrodis in controlling Bemsia tabaci populations].

The effectiveness of Eretmocerus sp. and Aschersonia aleyrodis in controlling Bemisia tabaci populations on cucumber were determined in separated and combined greenhouse experiments. The results showed that after spraying A. aleyrodis (5 x 10(6) spores per ml, twice per generation) and releasing Eretmocerus sp. (3 female per plant, twice per generation), 95.74% and 57.58% of whitefly populations were controlled, respectively. However, when the two natural enemies were used together with the same rate mentioned above (A. aleyrodis spraying twice followed by releasing Eretmocerus sp. once or twice in one generation), 97.02%-97.91% of whitefly populations were controlled. Moreover, the increasing index of whitefly population (I) subjected to both natural enemies together was below I, indicating the substantial reduction in whitefly population. The combined treatments provided a better control effect of whitefly than separated ones, and there was no contradicted effect between both natural enemies.