RESUMO
Natural habitats contain dynamic elements, such as varying local illumination. Can such features mitigate the salience of organism movement? Dynamic illumination is particularly prevalent in coral reefs, where patterns known as 'water caustics' play chaotically in the shallows. In behavioural experiments with a wild-caught reef fish, the Picasso triggerfish (Rhinecanthus aculeatus), we demonstrate that the presence of dynamic water caustics negatively affects the detection of moving prey items, as measured by attack latency, relative to static water caustic controls. Manipulating two further features of water caustics (sharpness and scale) implies that the masking effect should be most effective in shallow water: scenes with fine scale and sharp water caustics induce the longest attack latencies. Due to the direct impact upon foraging efficiency, we expect the presence of dynamic water caustics to influence decisions about habitat choice and foraging by wild prey and predators.
Assuntos
Cáusticos/toxicidade , Peixes/fisiologia , Poluentes da Água/toxicidade , Animais , Comportamento Animal , Recifes de Corais , Estimulação Luminosa , TetraodontiformesRESUMO
Coral reefs belong to the most diverse ecosystems on our planet. The diversity in coloration and lifestyles of coral reef fishes makes them a particularly promising system to study the role of visual communication and adaptation. Here, we investigated the evolution of visual pigment genes (opsins) in damselfish (Pomacentridae) and examined whether structural and expression variation of opsins can be linked to ecology. Using DNA sequence data of a phylogenetically representative set of 31 damselfish species, we show that all but one visual opsin are evolving under positive selection. In addition, selection on opsin tuning sites, including cases of divergent, parallel, convergent and reversed evolution, has been strong throughout the radiation of damselfish, emphasizing the importance of visual tuning for this group. The highest functional variation in opsin protein sequences was observed in the short- followed by the long-wavelength end of the visual spectrum. Comparative gene expression analyses of a subset of the same species revealed that with SWS1, RH2B and RH2A always being expressed, damselfish use an overall short-wavelength shifted expression profile. Interestingly, not only did all species express SWS1 - a UV-sensitive opsin - and possess UV-transmitting lenses, most species also feature UV-reflective body parts. This suggests that damsels might benefit from a close-range UV-based 'private' communication channel, which is likely to be hidden from 'UV-blind' predators. Finally, we found that LWS expression is highly correlated to feeding strategy in damsels with herbivorous feeders having an increased LWS expression, possibly enhancing the detection of benthic algae.
Assuntos
Proteínas de Peixes/genética , Opsinas/genética , Perciformes/genética , Visão Ocular , Animais , Perciformes/fisiologia , FilogeniaRESUMO
We describe the bi-directed eyes of a mesopelagic teleost fish, Rhynchohyalus natalensis, that possesses an extensive lateral diverticulum to each tubular eye. Each diverticulum contains a mirror that focuses light from the ventro-lateral visual field. This species can thereby visualize both downwelling sunlight and bioluminescence over a wide field of view. Modelling shows that the mirror is very likely to be capable of producing a bright, well focused image. After Dolichopteryx longipes, this is only the second description of an eye in a vertebrate having both reflective and refractive optics. Although superficially similar, the optics of the diverticular eyes of these two species of fish differ in some important respects. Firstly, the reflective crystals in the D. longipes mirror are derived from a tapetum within the retinal pigment epithelium, whereas in R. natalensis they develop from the choroidal argentea. Secondly, in D. longipes the angle of the reflective crystals varies depending on their position within the mirror, forming a Fresnel-type reflector, but in R. natalensis the crystals are orientated almost parallel to the mirror's surface and image formation is dependent on the gross morphology of the diverticular mirror. Two remarkably different developmental solutions have thus evolved in these two closely related species of opisthoproctid teleosts to extend the restricted visual field of a tubular eye and provide a well-focused image with reflective optics.
Assuntos
Olho/anatomia & histologia , Peixes/anatomia & histologia , Fenômenos Ópticos , Animais , Peixes/fisiologia , Oceanos e Mares , Visão Ocular , Campos VisuaisRESUMO
Many taxa use conspicuous colouration to attract mates, signal chemical defences (aposematism) or for thermoregulation. Conspicuousness is a key feature of aposematic signals, and experimental evidence suggests that predators avoid conspicuous prey more readily when they exhibit larger body size and/or pattern elements. Aposematic prey species may therefore evolve a larger body size due to predatory selection pressures, or alternatively, larger prey species may be more likely to evolve aposematic colouration. Therefore, a positive correlation between conspicuousness and body size should exist. Here, we investigated whether there was a phylogenetic correlation between the conspicuousness of animal patterns and body size using an intriguing, understudied model system to examine questions on the evolution of animal signals, namely nudibranchs (opisthobranch molluscs). We also used new ways to compare animal patterns quantitatively with their background habitat in terms of intensity variance and spatial frequency power spectra. In studies of aposematism, conspicuousness is usually quantified using the spectral contrast of animal colour patches against its background; however, other components of visual signals, such as pattern, luminance and spectral sensitivities of potential observers, are largely ignored. Contrary to our prediction, we found that the conspicuousness of body patterns in over 70 nudibranch species decreased as body size increased, indicating that crypsis was not limited to a smaller body size. Therefore, alternative selective pressures on body size and development of colour patterns, other than those inflicted by visual hunting predators, may act more strongly on the evolution of aposematism in nudibranch molluscs.
Assuntos
Comunicação Animal , Evolução Biológica , Tamanho Corporal , Gastrópodes/fisiologia , Pigmentação , Animais , Teorema de Bayes , Análise de RegressãoRESUMO
It might seem obvious that a camouflaged animal must generally match its background whereas to be conspicuous an organism must differ from the background. However, the image parameters (or statistics) that evaluate the conspicuousness of patterns and textures are seldom well defined, and animal coloration patterns are rarely compared quantitatively with their respective backgrounds. Here we examine this issue in the Australian giant cuttlefish Sepia apama. We confine our analysis to the best-known and simplest image statistic, the correlation in intensity between neighboring pixels. Sepia apama can rapidly change their body patterns from assumed conspicuous signaling to assumed camouflage, thus providing an excellent and unique opportunity to investigate how such patterns differ in a single visual habitat. We describe the intensity variance and spatial frequency power spectra of these differing body patterns and compare these patterns with the backgrounds against which they are viewed. The measured image statistics of camouflaged animals closely resemble their backgrounds, while signaling animals differ significantly from their backgrounds. Our findings may provide the basis for a set of general rules for crypsis and signals. Furthermore, our methods may be widely applicable to the quantitative study of animal coloration.
Assuntos
Fenômenos Ópticos , Pigmentação , Sepia , Adaptação Biológica , Comunicação Animal , Animais , Feminino , Análise de Fourier , Masculino , Percepção VisualRESUMO
The expression of cone opsin genes is a primary determinant of the characteristics of colour vision. Interspecific variation in opsin expression is common in African cichlids. It is correlated with foraging among cichlids from Lake Malawi, and with ambient light environment among cichlids from Lake Victoria. In this study, we tested whether gene expression varied within species such that it might be important in contributing to divergence. We hypothesized that light attenuation with depth would be correlated with predictable changes in gene expression in Lake Malawi, and that this variation would tune visual sensitivities to match the ambient light environment. We observed significant differences in cone opsin expression in three different comparisons among populations of the same species. Higher LWS expression was found in shallow versus deep Copadichromis eucinostomus. In Metriaclima zebra, individuals from Zimbawe Rock expressed significantly more SWS2B than those from Thumbi West Island, although these locales have similar ambient light environments. Finally, Tropheops gracilior from deeper water had significantly more variation in expression than their shallow counterparts. These results support that gene expression varies significantly between populations of the same species. Surprisingly, these results could not be explained by predicted visual performance as models predicted that differential expression patterns did not confer sensitivity advantages at different depths. This suggested that expression variation did not confer a local sensitivity advantage. Therefore, our findings were contrary to a primary requirement of the sensory bias hypothesis. As such, other explanations for intraspecific gene expression variation need to be tested.
Assuntos
Ciclídeos/genética , Opsinas dos Cones/genética , Expressão Gênica , Células Fotorreceptoras Retinianas Cones/metabolismo , Acuidade Visual/genética , África , Animais , Ciclídeos/fisiologia , Visão de Cores , Evolução Molecular , Água Doce , Variação Genética , Luz , Filogenia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
To investigate the distribution of thyroid-stimulating antibody (TSAb) activity between IgG subclasses, sera from 11 patients with Graves disease (including the National Institute of Biological Standards and Control (NIBSC) Research Standard, long acting thyroid stimulator-B) were fractionated by chromatography on affinity columns of monoclonal IgG subclass antibodies or protein A to deplete all but a single subclass. The resulting fractions were 98% or more pure for a single subclass. In all 11 patients, TSAb activity appeared to be confined to the IgG1 fraction as determined by cAMP production on addition of the fractions to the FRTL-5 rat thyroid cell line. In all of eight specimens from seven patients so tested, the whole serum activity was recovered in the IgG1 fraction, after adjusting for the recovery of the isotype from the column. TSAb activity in one serum comprised both lambda and kappa light chains but was IgG1 restricted. This IgG subclass restriction was not found when the same fractions were tested for thyroglobulin, microsomal/thyroid peroxidase, or tetanus toxoid antibody activity. Together with previous results showing marked restriction of both light chain usage and isoelectric point of TSAb, these results support the idea that Graves' disease may be the result of an oligo- or possibly monoclonal response at the B cell level.
Assuntos
Doença de Graves/imunologia , Imunoglobulina G/imunologia , Receptores da Tireotropina/imunologia , Glândula Tireoide/imunologia , AMP Cíclico/biossíntese , Humanos , Imunoglobulina G/classificação , Iodeto Peroxidase/imunologia , Toxoide Tetânico/imunologia , Tireoglobulina/imunologiaRESUMO
From patients with untreated Graves' disease 11 sera showing high cAMP release in the FRTL-5 cell assay were studied for relative proportions of kappa or lambda Ig molecules showing cAMP releasing activity. Immunoabsorption of gamma-globulins was performed using monoclonal murine anti-kappa or anti-lambda antibodies linked to cyanogen bromide-activated sepharose. Specific kappa- or lambda-adsorbed fractions were also eluted from immunoabsorbents using chaotrophic thiocyanate buffers and equilibrated with pH 7.4 low salt buffer by dialysis. Immunoabsorption and elution experiments showed that five Graves' sera contained predominant cAMP-releasing activity within lambda Ig fractions, whereas two Graves' sera showed predominant cAMP-releasing activity in kappa Ig fractions. Four sera showed cAMP release approximately equally divided between kappa and lambda Ig both after immunoabsorption and specific anti-kappa or anti-lambda eluates were studied. C lambda genotypes were examined by Southern blotting and restriction fragment length polymorphism analysis of Eco RI-digested genomic DNA from 158 patients with Graves' disease in parallel with 112 normal controls and 29 patients with autoimmune hypothyroidism. Notable shifts in proportions of 8/8 and 18/18 genotypes were present when Graves' patients were compared with normal controls. Allelic frequencies and ratios of genotype 8 to 18 were significantly different (P less than 0.05) when Graves' patients were compared either to normal controls or to patients with autoimmune hypothyroidism.
Assuntos
Genes de Imunoglobulinas , Doença de Graves/imunologia , Regiões Constantes de Imunoglobulina/genética , Imunoglobulina G/análise , Cadeias kappa de Imunoglobulina/análise , Cadeias lambda de Imunoglobulina/análise , Absorção , Autoanticorpos/análise , Autoanticorpos/genética , Genótipo , Doença de Graves/genética , Humanos , Regiões Constantes de Imunoglobulina/isolamento & purificação , Imunoglobulina G/genética , Cadeias kappa de Imunoglobulina/genética , Cadeias lambda de Imunoglobulina/genética , Imunoglobulinas Estimuladoras da Glândula Tireoide , Polimorfismo de Fragmento de RestriçãoRESUMO
After hatching, larvae of coral reef fishes experience a pelagic phase during which they are diurnal planktivores. It has been suggested that ultraviolet (UV) vision is beneficial for the detection of planktonic prey. Aims were therefore to investigate whether ocular media of pre-settlement reef fish differ from those of respective adults, and whether larvae have UV-transparent ocular media required for UV vision. The ocular media of 84 pre-settlement and 98 adult species belonging to the same families were measured and compared. We suggest that adult lifestyle rather than planktivory in general shapes the ocular media properties of pre-settlement larvae.
Assuntos
Percepção de Cores/fisiologia , Peixes/fisiologia , Raios Ultravioleta , Animais , Comportamento Alimentar , Peixes/crescimento & desenvolvimento , Larva/fisiologia , Cristalino/anatomia & histologia , Cristalino/fisiologia , Comportamento Predatório , Espalhamento de Radiação , Especificidade da EspécieRESUMO
Coral reef fishes are among the most colourful animals in the world. Given the diversity of lifestyles and habitats on the reef, it is probable that in many instances coloration is a compromise between crypsis and communication. However, human observation of this coloration is biased by our primate visual system. Most animals have visual systems that are 'tuned' differently to humans; optimized for different parts of the visible spectrum. To understand reef fish colours, we need to reconstruct the appearance of colourful patterns and backgrounds as they are seen through the eyes of fish. Here, the coral reef associated triggerfish, Rhinecanthus aculeatus, was tested behaviourally to determine the limits of its colour vision. This is the first demonstration of behavioural colour discrimination thresholds in a coral reef species and is a critical step in our understanding of communication and speciation in this vibrant colourful habitat. Fish were trained to discriminate between a reward colour stimulus and series of non-reward colour stimuli and the discrimination thresholds were found to correspond well with predictions based on the receptor noise limited visual model and anatomy of the eye. Colour discrimination abilities of both reef fish and a variety of animals can therefore now be predicted using the parameters described here.
RESUMO
Peptide transporters are present in all species to absorb the small peptides that occur ubiquitously as products of proteolysis. The broad substrate specificities of these systems allow them to be exploited therapeutically for delivery of peptidomimetic drugs in microbes and man. To this end, glycylsarcosine is currently used as a standard substrate for assaying peptidomimetic transport by peptide transporters. However, in this study we find it is unsuitable as a general substrate, based on assays of its transport by model bacterial peptide transporters and computer-based conformational analysis of its structure. Of the two generic transporters for di- and tripeptides, exemplified by Dpp and Tpp in Escherichia coli, only Dpp can transport glycylsarcosine. The explanation for this finding came from molecular modelling, which indicated that glycylsarcosine can adopt only a restricted range of conformers compared with typical dipeptides, and that of the conformers with a trans peptide bond, the majority have the specific psi and phi backbone torsion angles needed for molecular recognition and transport by Dpp but none possessed psi and phi torsions required for recognition by Tpp; moreover, 38% of its conformers have cis peptide bonds that are not substrates for any peptide transporter. Thus, using glycylsarcosine as substrate in competition assays with compounds that typically form conformers recognised by both types of peptide transporter will underestimate their transport. These findings have implications for assays of oral availability of peptidomimetic drugs such as beta-lactams, ACE inhibitors and anti-viral compounds, for which glycylsarcosine is routinely used.
Assuntos
Transportadores de Cassetes de Ligação de ATP/química , Dipeptídeos/química , Simulação por Computador , Escherichia coli , Conformação Proteica , Especificidade por Substrato , TermodinâmicaRESUMO
Deep-sea fish, defined as those living below 200 m, inhabit a most unusual photic environment, being exposed to two sources of visible radiation; very dim downwelling sunlight and bioluminescence, both of which are, in most cases, maximal at wavelengths around 450-500 nm. This paper summarises the reflective properties of the ocular tapeta often found in these animals, the pigmentation of their lenses and the absorption characteristics of their visual pigments. Deep-sea tapeta usually appear blue to the human observer, reflecting mainly shortwave radiation. However, reflection in other parts of the spectrum is not uncommon and uneven tapetal distribution across the retina is widespread. Perhaps surprisingly, given the fact that they live in a photon limited environment, the lenses of some deep-sea teleosts are bright yellow, absorbing much of the shortwave part of the spectrum. Such lenses contain a variety of biochemically distinct pigments which most likely serve to enhance the visibility of bioluminescent signals. Of the 195 different visual pigments characterised by either detergent extract or microspectrophotometry in the retinae of deep-sea fishes, ca. 87% have peak absorbances within the range 468-494 nm. Modelling shows that this is most likely an adaptation for the detection of bioluminescence. Around 13% of deep-sea fish have retinae containing more than one visual pigment. Of these, we highlight three genera of stomiid dragonfishes, which uniquely produce far red bioluminescence from suborbital photophores. Using a combination of longwave-shifted visual pigments and in one species (Malacosteus niger) a chlorophyll-related photosensitizer, these fish have evolved extreme red sensitivity enabling them to see their own bioluminescence and giving them a private spectral waveband invisible to other inhabitants of the deep-ocean.
Assuntos
Cristalinas/fisiologia , Peixes/fisiologia , Cristalino/fisiologia , Pigmentos Biológicos/fisiologia , Pigmentos da Retina/fisiologia , Animais , Cristalinas/química , Peixes/anatomia & histologia , Cristalino/química , Oceanos e Mares , Pigmentos Biológicos/química , Pigmentos da Retina/químicaRESUMO
TSH bound to human fat membranes is shown to be unusually sensitive to the addition of immunoglobulin G (IgG). Significant displacement was observed with 10 microgram/ml, and 50% displacement was obtained with 50-100 microgram/ml IgG prepared from control sera pooled from euthyroid subjects. It was not possible to demonstrate increased TSH displacement by IgG preparations from sera of thyrotoxic compared to that of euthyroid subjects using human fat membranes, as was demonstrable with human thyroid membranes. The increased sensitivity of fat membranes to IgG was not due to nonspecific protein interactions and is suggested to derive from interactions with Fc receptors associated with fat membranes.
Assuntos
Tecido Adiposo/metabolismo , Imunoglobulina G , Receptores de Superfície Celular/metabolismo , Hormônio Liberador de Tireotropina/metabolismo , Animais , Membrana Celular/metabolismo , Cobaias , Humanos , Hipertireoidismo/imunologia , Cinética , Masculino , Receptores Fc/metabolismo , Receptores da Tireotropina , Glândula Tireoide/metabolismoRESUMO
Propranolol (1 mM) was found to inhibit TSH stimulation of adenyl cyclase activity in a subcellular fraction from bovine thyroid enriched in plasma membranes. However, stimulation due to PGE1 or NaF was not similarly inhibited. Since (i) and inhibition was observed at concentrations of propranolol between 10-minus 4 and 10- minus 3M, and appeared to be noncompetitive (ii) the optical isomers of propranolol were equipotent, (iii) inhibition was specific for propranolol since it was not observed with the closely related drug practolol (1 mM), and (iv) quinidine (1 mM) and the local anaesthetics lignocaine and aptocaine also proved inhibitory, we concluded that propranolol inhibition of TSH stimulation was due to its "quinidine-like" properties (i.e., relatively specific and characteristic membrane-active properties) and not to its action as a beta-adrenergic antagonist.
Assuntos
Adenilil Ciclases/metabolismo , Propranolol/farmacologia , Glândula Tireoide/enzimologia , Tireotropina/fisiologia , Adenosina Trifosfatases/metabolismo , Anestésicos Locais/farmacologia , Animais , Bovinos , Membrana Celular/efeitos dos fármacos , Membrana Celular/enzimologia , Ativação Enzimática/efeitos dos fármacos , Fluoretos/farmacologia , Técnicas In Vitro , Lidocaína/farmacologia , Magnésio/farmacologia , Monoéster Fosfórico Hidrolases/metabolismo , Potássio/farmacologia , Practolol/farmacologia , Prostaglandinas E/farmacologia , Quinidina/farmacologia , Sódio/farmacologia , Estereoisomerismo , Tireotropina/farmacologiaRESUMO
Catecholamine stimulation of adenyl cyclase activity associated with bovine subcellular fractions enriched in plasma membranes is described. The relative potencies of isoproterenol (IPNA), epinephrine (E), and norepinephrine (NE) were 4.7:1.0:0.02, which is characteristic for a beta-adrenergic receptor system. Stimulation with IPNA (5 times 10- minus 6 M) was inhibited by propranolol. The inhibition was stereospecific for the L-isomer of propranolol and a concentration as low as 2 times 10- minus 8 M was required to effect 50% inhibition. Both these observations, and the competitive kinetics of inhibition which applied to the system, confirmed the classification of a beta-adrenergic receptor system. Phentolamine, an alpha-antagonist, also inhibited IPNA stimulation, although high doses (5 times 10- minus M) were required to cause total inhibition. Inhibition was also observed with quinidine (1 mM) and lignocaine (10- minus 2 M).
Assuntos
Adenilil Ciclases/metabolismo , Isoproterenol/farmacologia , Glândula Tireoide/enzimologia , Animais , Bovinos , Membrana Celular/efeitos dos fármacos , Membrana Celular/enzimologia , Ativação Enzimática/efeitos dos fármacos , Epinefrina/farmacologia , Técnicas In Vitro , Cinética , Lidocaína/farmacologia , Norepinefrina/farmacologia , Fentolamina/farmacologia , Propranolol/farmacologia , Quinidina/farmacologia , Fatores de TempoRESUMO
Selected clones of monoclonal antibodies from mice immunized with solubilized preparations of bovine TSH receptors have been characterized in a cytochemical bioassay (CBA) for thyroid stimulators. This assay is based upon quantification of changes in naphthylamidase activity of sections of guinea pig thyroids with use of a chromogenic substrate. Monoclonal 22A6 is a thyroid-stimulating antibody directed at a site within the TSH receptor. Thus, although it is a weak inhibitor of 125I-TSH binding to thyroid membranes, 22A6 is inhibited from binding to membranes by TSH, exhibits a more than additive agonist effect on adenylate cyclase activity when tested at low TSH concentrations in thyroid cells, and is a competitive antagonist of TSH enhancement of adenylate cyclase activity at high TSH concentrations. In the CBA, 22A6 is a stimulator whose maximal activity is obtained with 77 pg/ml (3-min exposure). Dose-response curves of a long acting thyroid stimulator (LATS)-B standard and 22A6 have slopes which are not significantly different; as anticipated, the response to LATS-B is inhibited by antihuman immunoglobulin G (IgG) and that due to 22A6 by antimouse IgG. In contrast to 22A6, monoclonal 11E8 is a relatively potent inhibitor of 125I-TSH binding as well as TSH stimulation of adenylate cyclase activity, while failing to act as a stimulator itself. 11E8 is itself inactive as a stimulator in the CBA over a wide dose range; it does, however, inhibit TSH stimulation in the CBA. This inhibition is abolished by antimouse IgG. The transient peak of response observed in time courses to TSH occurs later in the presence of 11E8. Unlike its effect on TSH 11E8 shows relatively low potency (greater than 10,000-fold lower) when inhibiting stimulation by the thyroid stimulating antibodies, 22A6 or LATS-B. Since this difference cannot be explained by quantitative differences in the ability of 22A6 or 11E8 to bind to thyroid membranes, the CBA data suggest that the stimulating antibodies, 22A6 and LATS-B, may interact with different determinants on TSH receptors then either TSH or the blocking antibody, 11E8. This also implies that in Graves' disease blocking antibodies may be incompletely expressed in the presence of stimulating antibodies, although they may be potent inhibitors of TSH binding, as measured in receptor assays.
Assuntos
Anticorpos Monoclonais/fisiologia , Anticorpos/análise , Bioensaio/métodos , Receptores de Superfície Celular/imunologia , Adenilil Ciclases/metabolismo , Animais , Anticorpos/imunologia , Anticorpos/fisiologia , Anticorpos Anti-Idiotípicos/imunologia , Anticorpos Monoclonais/imunologia , Bovinos , AMP Cíclico/biossíntese , Doença de Graves/imunologia , Cobaias , Imunoglobulina G/imunologia , Imunoglobulinas Estimuladoras da Glândula Tireoide , Estimulador Tireóideo de Ação Prolongada/farmacologia , Camundongos , Ratos , Receptores da Tireotropina , Glândula Tireoide/imunologia , Glândula Tireoide/metabolismo , Tireotropina/antagonistas & inibidores , Tireotropina/farmacologiaRESUMO
The effects of ionic zinc (Zn2+) on human (h) GH bioactivity have been examined using a lactogenic bioassay. The potencies of pituitary-derived hGH (IRP 80/505), recombinant 22K hGH (IRP 88/624), pituitary-derived human PRL (IRP 84/500), and a recombinant methionyl 20-kilodalton variant of hGH in the presence of selected concentrations of ZnCl2 were investigated with an eluted stain assay that uses Nb2 rat lymphoma cells. This precise colorimetric bioassay is based upon the reduction of a yellow tetrazolium salt, 3-[4,5-dimethyl-thiazol-2-yl]2,5-di-phenyl-tetrazolium bromide, to its purple formazan by lactogen-activated Nb2 cells. Zinc (6-100 microM) enhanced the bioactivity of low doses (< 0.045 nM) of both pituitary-derived and recombinant 22K hGH, although the magnitude of enhancement was considerably less than might have been anticipated from previous binding studies (13). Higher concentrations of pituitary-derived hGH (> 0.045 nM) were inhibited by Zn2+. The bioactivity of recombinant methionyl 20K hGH was greatly enhanced by zinc (3-100 microM). In contrast to hGH, the bioactivity of hPRL was not potentiated by Zn2+. These discriminatory effects of Zn2+ when stimulating via the lactogenic receptor are in concordance with the results of previous radioligand binding studies (13). The striking enhancement of 20K hGH lactogenic bioactivity was observed at Zn2+ concentrations within the physiological range for normal human serum (5-20 microM).
Assuntos
Bioensaio/métodos , Hormônio do Crescimento/metabolismo , Zinco/farmacologia , Animais , Cobalto/farmacologia , Colorimetria , Cobre/farmacologia , Hormônio do Crescimento/farmacologia , Humanos , Concentração Osmolar , Hipófise/metabolismo , Prolactina/farmacologia , Proteínas Recombinantes , Células Tumorais Cultivadas/metabolismoRESUMO
The time course of response of thyroid sections in the cytochemical bioassay to either TSH or thyroid-stimulating antibodies is bell shaped. The maximal staining for lysosomal naphthylamidase activity achieved was found to be the same regardless of the dose of stimulator applied; however, the rate at which the maximum was attained was dose dependent. Sections exposed to 10(-1) mU/liter TSH showed a maximal response at 120 sec, and those exposed to 10(-3) mU/liter TSH showed a maximal response at 210 sec. A similar dose-time effect was seen with immunoglobulin G from a thyrotoxic patient. Thus, by selecting a specific exposure time, a dose-response curve to the stimulator was obtained. A dose-response curve to a range of concentrations from 10(-4)-10(-1) mU/liter TSH was obtained by exposing sections to the hormone for 90 sec. TSH (10(-3) mU/liter) produced a response significantly different (P less than 0.0025) from the control. However, 10(-5) mU/liter TSH produced a response significantly different (P less than 0.0025) from the control after an exposure time of 180 sec, and the range of the dose-response curve at this exposure time was 10(-6)-10(-3) mU/liter TSH. Each point on these two dose-response curves was determined in quintuplicate, and precision profiles were constructed. The assay performed at 90 sec had a lower relative precision of 30% at a dose of 10(-1) mU/liter TSH, and at 180 sec, the best lower relative precision achieved was 80%. Thus, the sensitivity of the assay was improved by increasing the exposure time of the sections to TSH, but with a resultant loss of relative precision.
Assuntos
Anticorpos/fisiologia , Glândula Tireoide/fisiologia , Tireotropina/farmacologia , Animais , Bioensaio , Relação Dose-Resposta a Droga , Relação Dose-Resposta Imunológica , Cobaias , Humanos , Hipertireoidismo/imunologia , Imunoglobulina G/farmacologia , Imunoglobulinas Estimuladoras da Glândula Tireoide , Fatores de TempoRESUMO
The cAMP response to TSH was investigated in slice preparations of normal human thyroid tissue maintained under in vitro conditions. In particular, dose-response relationships for tissue obtained from different thyroid glands were assessed, and the sensitivity and precision of this system were compared with the adenylate cyclase response to TSH in plasma membrane preparations derived from similar tissue. The effect of thyroid-stimulating antibodies (TSAb) on the thyroid slice cAMP response was investigated. After an incubation period of 120 min, 58% of a series of 43 immunoglobulin G (IgG) fractions prepared from the sera of Graves' disease patients significantly elevated the slice level of cAMP when assayed in triplicate at a level of 5 mg/ml. From a total of 22 preparations of IgG from untreated thyrotoxic patients, 11 (50%) stimulated the accumulation of cAMP in thyroid slices. Six out of a group of 8 patients with Graves' ophthalmopathy had demonstrable TSAb activity. After the initiation of antithyroid therapy with carbimazole, IgG preparations from 5 out of a group of 8 patients were without detectable TSAb activity. However, cAMP accumulation was stimulated by all 5 of a group of IgG preparations from previously thyrotoxic patients who had relapsed upon cessation of antithyroid therapy. The elevated cAMP levels in tissue slices in response to either TSH or TSAb were accompanied by an increase in cAMP in the incubation medium. The magnitude and significance of this extracellular cAMP accumulation were similar to those observed in the tissue and, accordingly, may form the basis of a simplified bioassay procedure for TSAb.