RESUMO
The occurrence of Giardia and Cryptosporidium was investigated in cetacean specimens stranded on the northwestern coast of Spain (European Atlantic coast) by analysis of 65 samples of large intestine from eight species. The parasites were identified by direct immunofluorescence antibody test (IFAT) and by PCR amplification of the ß-giardin gene, the ITS1-5.8S-ITS2 region and the SSU-rDNA gene of Giardia and the SSU-rDNA gene of Cryptosporidium. Giardia and Cryptosporidium were detected in 7 (10.8 %) and 9 samples (13.8 %), respectively. In two samples, co-infection with both parasites was observed. Giardia duodenalis assemblages A, C, D and F, and Cryptosporidium parvum were identified. This is the first report of G. duodenalis in Balaenoptera acutorostrata, Kogia breviceps and Stenella coeruleoalba and also the first report of Cryptosporidium sp. in B. acutorostrata and of C. parvum in S. coeruleoalba and Tursiops truncatus. These results extend the known host range of these waterborne enteroparasites.
Assuntos
Coinfecção , Criptosporidiose/epidemiologia , Cryptosporidium/isolamento & purificação , Doenças dos Peixes/epidemiologia , Giardia/isolamento & purificação , Giardíase/veterinária , Animais , Oceano Atlântico/epidemiologia , Sequência de Bases , Cetáceos , Coinfecção/veterinária , Criptosporidiose/parasitologia , Cryptosporidium/classificação , Cryptosporidium/genética , Cryptosporidium parvum/genética , DNA de Protozoário/química , DNA de Protozoário/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Feminino , Doenças dos Peixes/parasitologia , Giardia/classificação , Giardia/genética , Giardia lamblia/genética , Giardíase/epidemiologia , Giardíase/parasitologia , Humanos , Masculino , Dados de Sequência Molecular , Análise de Sequência de DNA/veterinária , Espanha/epidemiologiaRESUMO
The cyamids collected from a True's beaked whale (Mesoplodon mirus), a common dolphin (Delphinus delphis), and a harbour porpoise (Phocoena phocoena) stranded in Galicia (NW Spain) were identified as Isocyamus deltobranchium, extending the range of this species to the Atlantic Ocean. The three cetacean species examined are new hosts for this parasite. The microscopic examination of female specimens reveals that certain important taxonomic characters can be present or absent depending on the maturation stage, an aspect which should be considered in future studies describing cyamid species.