RESUMO
We report a case of a 13-year-old girl with soft tissue sarcoma of the hand, which showed muscle and neuroectodermal immunophenotypes. Molecular studies were performed on RNA collected from fine-needle aspiration (FNA) cytology and peripheral blood samples by nested reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blot analysis. This biphenotypic tumor showed simultaneous expression of EWS-FLI1 and PAX3-FKHR transcripts, specific of Ewing family tumors and alveolar rhabdomyosarcoma, respectively. Although childhood sarcomas with simultaneous muscle and neural differentiation have been described to have EWS-FLI1 transcripts, there are no reports of tumors with both transcripts. Cytological specimens are a good source of RNA for molecular studies.
Assuntos
Neoplasias Musculares/patologia , Tumores Neuroectodérmicos Primitivos Periféricos/patologia , Sarcoma de Células Pequenas/patologia , Adolescente , Biomarcadores Tumorais/análise , Southern Blotting , Quimera/genética , Feminino , Mãos , Humanos , Imuno-Histoquímica , Imunofenotipagem , Hibridização in Situ Fluorescente , Imageamento por Ressonância Magnética , Neoplasias Musculares/genética , Neoplasias Musculares/imunologia , Músculo Esquelético/imunologia , Músculo Esquelético/patologia , Tumores Neuroectodérmicos Primitivos Periféricos/genética , Tumores Neuroectodérmicos Primitivos Periféricos/imunologia , Reação em Cadeia da Polimerase , RNA Neoplásico/análise , Sarcoma de Células Pequenas/genética , Sarcoma de Células Pequenas/imunologiaRESUMO
Medullary thyroid carcinoma (MTC) is a rare malignancy which spreads frequently to cervical lymph nodes. We report the CT findings of MTC metastatic cervical adenopathies in two patients with previously resected MTC. The CT scans showed calcifications (one patient) and massive homogeneous postcontrast nodal enhancement. Medullary thyroid carcinoma should be included in the differential diagnosis of entities showing calcifications and intense homogeneous adenopathic enhancement on CT studies.