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Biochem Cell Biol ; 102(3): 238-251, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38408323

RESUMO

Insects are the largest group of animals when it comes to the number and diversity of species. Yet, with the exception of Drosophila, no information is currently available on the primary structure of their sperm nuclear basic proteins (SNBPs). This paper represents the first attempt in this regard and provides information about six species of Neoptera: Poecillimon thessalicus, Graptosaltria nigrofuscata, Apis mellifera, Nasonia vitripennis, Parachauliodes continentalis, and Tribolium castaneum. The SNBPs of these species were characterized by acetic acid urea gel electrophoresis (AU-PAGE) and high-performance liquid chromatography fractionated. Protein sequencing was obtained using a combination of mass spectrometry sequencing, Edman N-terminal degradation sequencing and genome mining. While the SNBPs of several of these species exhibit a canonical arginine-rich protamine nature, a few of them exhibit a protamine-like composition. They appear to be the products of extensive cleavage processing from a precursor protein which are sometimes further processed by other post-translational modifications that are likely involved in the chromatin transitions observed during spermiogenesis in these organisms.


Assuntos
Sequência de Aminoácidos , Protaminas , Animais , Masculino , Protaminas/metabolismo , Protaminas/química , Proteínas Nucleares/metabolismo , Proteínas Nucleares/genética , Proteínas de Insetos/metabolismo , Proteínas de Insetos/química , Proteínas de Insetos/genética , Insetos/metabolismo , Dados de Sequência Molecular , Espermatozoides/metabolismo
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