RESUMO
The origin of the vertebrate head is one of the great unresolved issues in vertebrate evolutionary developmental biology. Although many of the novelties in the vertebrate head and pharynx derive from the neural crest, it is still unknown how early vertebrates patterned the neural crest within the ancestral body plan they inherited from invertebrate chordates. Here, using a basal vertebrate, the sea lamprey, we show that homologs of Semaphorin3F (Sema3F) ligand and its Neuropilin (Nrp) receptors show complementary and dynamic patterns of expression that correlate with key periods of neural crest development (migration and patterning of cranial neural crest-derived structures). Using CRISPR/Cas9-mediated mutagenesis, we demonstrate that lamprey Sema3F is essential for patterning of neural crest-derived melanocytes, cranial ganglia and the head skeleton, but is not required for neural crest migration or patterning of trunk neural crest derivatives. Based on comparisons with jawed vertebrates, our results suggest that the deployment of Nrp-Sema3F signaling, along with other intercellular guidance cues, was pivotal in allowing early vertebrates to organize and pattern cranial neural crest cells into many of the hallmark structures that define the vertebrate head.
Assuntos
Padronização Corporal , Cabeça/embriologia , Crista Neural/embriologia , Neuropilinas/metabolismo , Semaforinas/metabolismo , Transdução de Sinais , Animais , Sistemas CRISPR-Cas , Movimento Celular , Regulação da Expressão Gênica no Desenvolvimento , Técnicas de Inativação de Genes , Lampreias/genética , Melanócitos/citologia , Melanócitos/metabolismo , Crista Neural/citologia , Neuropilinas/genética , Filogenia , Semaforinas/genética , Células Receptoras Sensoriais/metabolismo , Crânio/citologiaRESUMO
Vertebrates possess paired cranial sensory ganglia derived from two embryonic cell populations, neural crest and placodes. Cranial sensory ganglia arose prior to the divergence of jawed and jawless vertebrates, but the developmental mechanisms that facilitated their evolution are unknown. Using gene expression and cell lineage tracing experiments in embryos of the sea lamprey, Petromyzon marinus, we find that in the cranial ganglia we targeted, development consists of placode-derived neuron clusters in the core of ganglia, with neural crest cells mostly surrounding these neuronal clusters. To dissect functional roles of neural crest and placode cell associations in these developing cranial ganglia, we used CRISPR/Cas9 gene editing experiments to target genes critical for the development of each population. Genetic ablation of SoxE2 and FoxD-A in neural crest cells resulted in differentiated cranial sensory neurons with abnormal morphologies, whereas deletion of DlxB in cranial placodes resulted in near-total loss of cranial sensory neurons. Taken together, our cell-lineage, gene expression, and gene editing results suggest that cranial neural crest cells may not be required for cranial ganglia specification but are essential for shaping the morphology of these sensory structures. We propose that the association of neural crest and placodes in the head of early vertebrates was a key step in the organization of neurons and glia into paired sensory ganglia.
Assuntos
Gânglios Sensitivos/crescimento & desenvolvimento , Lampreias/crescimento & desenvolvimento , Crista Neural/crescimento & desenvolvimento , Neurogênese , Animais , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Gânglios Sensitivos/citologia , Gânglios Sensitivos/metabolismo , Lampreias/metabolismo , Neuroglia/citologia , Neuroglia/metabolismo , Neurônios/citologia , Neurônios/metabolismo , Fatores de Transcrição SOX/genética , Fatores de Transcrição SOX/metabolismo , Crânio/crescimento & desenvolvimentoRESUMO
A major challenge in vertebrate evolution is to identify the gene regulatory mechanisms that facilitated the origin of neural crest cells and placodes from ancestral precursors in invertebrates. Here, we show in lamprey, a primitively jawless vertebrate, that the transcription factor Snail is expressed simultaneously throughout the neural plate, neural plate border, and pre-placodal ectoderm in the early embryo and is then upregulated in the CNS throughout neurogenesis. Using CRISPR/Cas9-mediated genome editing, we demonstrate that Snail plays functional roles in all of these embryonic domains or their derivatives. We first show that Snail patterns the neural plate border by repressing lateral expansion of Pax3/7 and activating nMyc and ZicA. We also present evidence that Snail is essential for DlxB-mediated establishment of the pre-placodal ectoderm but is not required for SoxB1a expression during formation of the neural plate proper. At later stages, Snail regulates formation of neural crest-derived and placode-derived PNS neurons and controls CNS neural differentiation in part by promoting cell survival. Taken together with established functions of invertebrate Snail genes, we identify a pan-bilaterian mechanism that extends to jawless vertebrates for regulating neurogenesis that is dependent on Snail transcription factors. We propose that ancestral vertebrates deployed an evolutionarily conserved Snail expression domain in the CNS and PNS for neurogenesis and then acquired derived functions in neural crest and placode development by recruitment of regulatory genes downstream of neuroectodermal Snail activity. Our results suggest that Snail regulatory mechanisms in vertebrate novelties such as the neural crest and placodes may have emerged from neurogenic roles that originated early in bilaterian evolution.
Assuntos
Evolução Biológica , Lampreias/embriologia , Lampreias/genética , Crista Neural/metabolismo , Neurogênese , Fatores de Transcrição da Família Snail/metabolismo , Animais , Diferenciação Celular/genética , Sobrevivência Celular/genética , Ectoderma/embriologia , Ectoderma/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Modelos Biológicos , Sistema Nervoso/embriologia , Sistema Nervoso/metabolismo , Neurogênese/genética , Neurônios/citologia , Filogenia , Fatores de Transcrição da Família Snail/genéticaRESUMO
Lampreys and hagfishes are the only surviving relicts of an ancient but ecologically dominant group of jawless fishes that evolved in the seas of the Cambrian era over half a billion years ago. Because of their phylogenetic position as the sister group to all other vertebrates (jawed vertebrates), comparisons of embryonic development between jawless and jawed vertebrates offers researchers in the field of evolutionary developmental biology the unique opportunity to address fundamental questions related to the nature of our earliest vertebrate ancestors. Here, we describe how genetic analysis of embryogenesis in the sea lamprey (Petromyzon marinus) has provided insight into the origin and evolution of developmental-genetic programs in vertebrates. We focus on recent work involving CRISPR/Cas9-mediated genome editing to study gene regulatory mechanisms involved in the development and evolution of neural crest cells and new cell types in the vertebrate nervous system, and transient transgenic assays that have been instrumental in dissecting the evolution of cis-regulatory control of gene expression in vertebrates. Finally, we discuss the broad potential for these functional genomic tools to address previously unanswerable questions related to the evolution of genomic regulatory mechanisms as well as issues related to invasive sea lamprey population control.
Assuntos
Feiticeiras (Peixe) , Petromyzon , Animais , Evolução Molecular , Lampreias/genética , Petromyzon/genética , Filogenia , VertebradosRESUMO
Glial cells in the nervous system regulate and support many functions related to neuronal activity. Understanding how the vertebrate nervous system has evolved demands a greater understanding of the mechanisms controlling evolution and development of glial cells in basal vertebrates. Among vertebrate glia, oligodendrocytes form an insulating myelin layer surrounding axons of the central nervous system (CNS) in jawed vertebrates. Jawless vertebrates lack myelinated axons but it is unclear when oligodendrocytes or the regulatory mechanisms controlling their development evolved. To begin to investigate the evolution of mechanisms controlling glial development, we identified key genes required for the differentiation of oligodendrocytes in gnathostomes, including Nkx2.2, SoxE genes, and PDGFR, analyzed their expression, and used CRISPR/Cas9 genome editing to perturb their functions in a primitively jawless vertebrate, the sea lamprey. We show in lamprey that orthologs required for oligodendrocyte development in jawed vertebrates are expressed in the lamprey ventral neural tube, in similar locations where gnathostome oligodendrocyte precursor cells (OPC) originate. In addition, they appear to be under the control of conserved mechanisms that regulate OPC development in jawed vertebrates and may also function in gliogenesis. Our results suggest that although oligodendrocytes first emerged in jawed vertebrates, regulatory mechanisms required for their development predate the divergence of jawless and jawed vertebrates.
Assuntos
Embrião não Mamífero/embriologia , Proteínas de Peixes , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Lampreias , Tubo Neural/embriologia , Neuroglia/metabolismo , Oligodendroglia/metabolismo , Animais , Embrião não Mamífero/citologia , Proteínas de Peixes/biossíntese , Proteínas de Peixes/genética , Lampreias/embriologia , Lampreias/genética , Tubo Neural/citologia , Neuroglia/citologia , Oligodendroglia/citologiaRESUMO
The acquisition of neural crest cells was a key step in the origin of the vertebrate body plan. An outstanding question is how neural crest cells acquired their ability to undergo an epithelial-mesenchymal transition (EMT) and migrate extensively throughout the vertebrate embryo. We tested if differential regulation of classical cadherins-a highly conserved feature of neural crest EMT and migration in jawed vertebrates-mediates these cellular behaviors in lamprey, a basal jawless vertebrate. Lamprey has single copies of the type I and type II classical cadherins (CadIA and CadIIA). CadIIA is expressed in premigratory neural crest, and requires the transcription factor Snail for proper expression, yet CadIA is never expressed in the neural tube during neural crest development, suggesting that differential regulation of classical cadherin expression is not required to initiate neural crest migration in basal vertebrates. We hypothesize that neural crest cells evolved by retention of regulatory programs linking distinct mesenchymal and multipotency properties, and emigrated from the neural tube without differentially regulating type I/type II cadherins. Our results point to the coupling of mesenchymal state and multipotency as a key event facilitating the origin of migratory neural crest cells.
Assuntos
Caderinas/metabolismo , Movimento Celular/fisiologia , Lampreias/embriologia , Crista Neural/embriologia , Fatores de Transcrição da Família Snail/metabolismo , Sequência de Aminoácidos , Animais , Caderinas/genética , Diferenciação Celular/fisiologia , Transição Epitelial-Mesenquimal/fisiologia , Regulação da Expressão Gênica no Desenvolvimento , Crista Neural/citologia , Tubo Neural/metabolismo , Alinhamento de SequênciaRESUMO
Vertebrate SoxE genes (Sox8, 9, and 10) are key regulators of neural crest cell (NCC) development. These genes arose by duplication from a single SoxE gene in the vertebrate ancestor. Although SoxE paralogs are coexpressed early in NCC development, later, Sox9 is restricted to skeletogenic lineages in the head, and Sox10 to non-skeletogenic NCC in the trunk and head. When this subfunctionalization evolved and its possible role in the evolution of the neural crest are unknown. Sea lampreys are basal vertebrates that also possess three SoxE genes, while only a single SoxE is present in the cephalochordate amphioxus. In order to address the functional divergence of SoxE genes, and to determine if differences in their biochemical functions may be linked to changes in neural crest developmental potential, we examined the ability of lamprey and amphioxus SoxE genes to regulate differentiation of NCC derivatives in zebrafish colourless (cls) mutants lacking expression of sox10. Our findings suggest that the proto-vertebrate SoxE gene possessed both melanogenic and neurogenic capabilities prior to SoxE gene duplication. Following the agnathan-gnathostome split, lamprey SoxE1 and SoxE3 largely lost their melanogenic and/or enteric neurogenic properties, while gnathostome SoxE paralogs have retained functional conservation. We posit that this difference in protein subfunctionalization is a direct consequence of the independent regulation of SoxE paralog expression between the two lineages. Specifically, we propose that the overlapping expression of gnathostome SoxE paralogs in early neural crest largely constrained the function of gnathostome SoxE proteins. In contrast, the largely non-overlapping expression of lamprey SoxE paralogs allowed them to specialize with regard to their DNA-binding and/or protein interaction properties. Restriction of developmental potential among cranial and trunk neural crest in lampreys may be related to constraints on SoxE activity among duplicates, but such specialization does not appear to have occurred in gnathostomes. This highlights an important difference in the evolution of SoxE activity between these two divergent vertebrate lineages and provides insights for understanding how cell fate restriction in different NCC populations may be dependent on subfunctionalization among SoxE duplicates.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Lampreias/embriologia , Anfioxos/embriologia , Crista Neural/embriologia , Fatores de Transcrição SOXE/genética , Proteínas de Peixe-Zebra/genética , Peixe-Zebra/embriologia , Animais , Evolução Biológica , Diferenciação Celular/genética , Duplicação Gênica/genética , Fator de Transcrição Associado à Microftalmia/biossíntese , Crista Neural/citologia , Neurogênese/genética , Proteínas de Peixe-Zebra/biossínteseRESUMO
Classical hypotheses regarding the evolutionary origin of paired appendages propose transformation of precursor structures (gill arches and lateral fin folds) into paired fins. During development, gnathostome paired appendages form as outgrowths of body wall somatopleure, a tissue composed of somatic lateral plate mesoderm (LPM) and overlying ectoderm. In amniotes, LPM contributes connective tissue to abaxial musculature and forms ventrolateral dermis of the interlimb body wall. The phylogenetic distribution of this character is uncertain because lineage analyses of LPM have not been generated in anamniotes. We focus on the evolutionary history of the somatopleure to gain insight into the tissue context in which paired fins first appeared. Lampreys diverged from other vertebrates before the acquisition of paired fins and provide a model for investigating the preappendicular condition. We present vital dye fate maps that suggest the somatopleure is eliminated in lamprey as the LPM is separated from the ectoderm and sequestered to the coelomic linings during myotome extension. We also examine the distribution of postcranial mesoderm in catshark and axolotl. In contrast to lamprey, our findings support an LPM contribution to the trunk body wall of these taxa, which is similar to published data for amniotes. Collectively, these data lead us to hypothesize that a persistent somatopleure in the lateral body wall is a gnathostome synapomorphy, and the redistribution of LPM was a key step in generating the novel developmental module that ultimately produced paired fins. These embryological criteria can refocus arguments on paired fin origins and generate hypotheses testable by comparative studies on the source, sequence, and extent of genetic redeployment.
Assuntos
Nadadeiras de Animais/embriologia , Evolução Biológica , Derme/embriologia , Lampreias/embriologia , Mesoderma/embriologia , Ambystoma mexicanum/embriologia , Animais , Linhagem da Célula/fisiologia , Crioultramicrotomia , Proteínas de Fluorescência Verde/metabolismo , Imuno-Histoquímica , Hibridização In Situ , Filogenia , Tubarões/embriologiaRESUMO
Lampreys, one of the two surviving groups of ancient vertebrates, have become important models for study in diverse fields of biology. Lampreys (of which there are approximately 40 species) are being studied, for example, (a) to control pest sea lamprey in the North American Great Lakes and to restore declining populations of native species elsewhere; (b) in biomedical research, focusing particularly on the regenerative capability of lampreys; and (c) by developmental biologists studying the evolution of key vertebrate characters. Although a lack of genetic resources has hindered research on the mechanisms regulating many aspects of lamprey life history and development, formerly intractable questions are now amenable to investigation following the recent publication of the sea lamprey genome. Here, we provide an overview of the ways in which genomic tools are currently being deployed to tackle diverse research questions and suggest several areas that may benefit from the availability of the sea lamprey genome.
RESUMO
SoxE genes are multifunctional transcriptional regulators that play key roles in specification and differentiation of neural crest. Three members (Sox8, Sox9, Sox10) are expressed in the neural crest and are thought to modulate the expression and activity of each other. In addition to regulating the expression of other early neural crest marker genes, SoxE genes are required for development of cartilage. Here we investigated the role of SoxE genes in development of the neural crest-derived branchial skeleton in the sea lamprey. Using a morpholino knockdown approach, we show that all three SoxE genes described in lamprey are required for branchial basket development. Our results suggest that SoxE1 and SoxE2 are required for specification of the chondrogenic neural crest. SoxE3 plays a morphogenetic role in patterning of the branchial basket and may be required for the development of mucocartilage, a tissue unique to larval lampreys. While the lamprey branchial basket develops primarily from an elastin-like major extracellular matrix protein that is specific to lampreys, fibrillar collagen is also expressed in developing branchial cartilage and may be regulated by the lamprey SoxE genes. Our data suggest that the regulation of Type II collagen by Sox9 might have been co-opted by the neural crest in development of the branchial skeleton following the divergence of agnathan and gnathostome vertebrates. Finally, our results also have implications for understanding the independent evolution of duplicated SoxE genes among agnathan and gnathostome vertebrates.
Assuntos
Duplicação Gênica , Lampreias/genética , Fatores de Transcrição SOXE/genética , Animais , Sequência de Bases , Colágeno Tipo II/metabolismo , Primers do DNA , Técnicas de Silenciamento de Genes , Lampreias/embriologia , Reação em Cadeia da Polimerase em Tempo RealRESUMO
The neural crest, a defining character of vertebrates, is of prime importance to their evolutionary origin. To understand neural crest evolution, we explored molecular mechanisms underlying craniofacial development in the basal jawless vertebrate, sea lamprey (Petromyzon marinus), focusing on the SoxE (Sox8, Sox9 and Sox10) gene family. In jawed vertebrates, these are important transcriptional regulators of the neural crest, and the loss of Sox9 causes abnormal craniofacial development. Here we report that two lamprey SoxE genes are expressed in migrating neural crest and crest-derived prechondrocytes in posterior branchial arches, whereas a third paralogue is expressed later in the perichondrium and mandibular arch. Morpholino knock-down of SoxE1 reveals that it is essential for posterior branchial arch development, although the mandibular arch is unaffected. The results show that chondrogenic function of SoxE regulators can be traced to the lamprey-gnathostome common ancestor and indicate that lamprey SoxE genes might have undergone independent duplication to have distinct functions in mandibular versus caudal branchial arches. This work sheds light on the homology of vertebrate branchial arches and supports their common origin at the base of vertebrates.
Assuntos
Evolução Biológica , Proteínas de Peixes/metabolismo , Crista Neural/embriologia , Crista Neural/metabolismo , Petromyzon/embriologia , Faringe/embriologia , Faringe/metabolismo , Animais , Região Branquial/citologia , Região Branquial/embriologia , Região Branquial/metabolismo , Proteínas de Peixes/genética , Dados de Sequência Molecular , Crista Neural/citologia , Petromyzon/genética , Faringe/citologiaRESUMO
Evolution of the skeleton was a key transition in early vertebrates. Lampreys lack a mineralized skeleton but possess cartilaginous neurocranial and viscerocranial elements. In lampreys, the visceral skeleton develops as a fused branchial basket supporting the pharynx. Here, we have adapted Alcian blue staining of lamprey cartilage and show this method results in cartilage fluorescence that we used to describe development of the branchial skeleton in Petromyzon marinus between 17 and 63 days of development. We show that skeletal rods develop from condensations of flattened discoidal chondrocytes and may involve cellular intercalation. Lamprey trabecular, parachordal, and subchordal cartilages consist of aggregations of polygonal chondrocytes positioned on the ventral and lateral surfaces of the notochord. We speculate that morphological differences relate to functional differences in the cartilage. We show that differentiated skeletal rods are derived from neural crest. Finally, we show how branchial muscles intercalate with skeletal rods of the branchial basket.
Assuntos
Osso e Ossos/embriologia , Petromyzon/embriologia , Crânio/embriologia , Vísceras/embriologia , Animais , Aquicultura , Desenvolvimento Ósseo/fisiologia , Região Branquial/embriologia , Cartilagem/embriologia , Condrogênese/fisiologia , Embrião não Mamífero , Desenvolvimento Embrionário/fisiologia , Modelos Biológicos , Espectrometria de FluorescênciaRESUMO
The neural crest is a vertebrate-specific migratory stem cell population that generates a remarkably diverse set of cell types and structures. Because many of the morphological, physiological and behavioural novelties of vertebrates are derived from neural crest cells, it is thought that the origin of this cell population was an important milestone in early vertebrate history. An outstanding question in the field of vertebrate evolutionary-developmental biology (evo-devo) is how this cell type evolved in ancestral vertebrates. In this review, we briefly summarize neural crest developmental genetics in vertebrates, focusing in particular on the gene regulatory interactions instructing their early formation within and migration from the dorsal neural tube. We then discuss how studies searching for homologues of neural crest cells in invertebrate chordates led to the discovery of neural crest-like cells in tunicates and the potential implications this has for tracing the pre-vertebrate origins of the neural crest population. Finally, we synthesize this information to propose a model to explain the origin of neural crest cells. We suggest that at least some of the regulatory components of early stages of neural crest development long pre-date vertebrate origins, perhaps dating back to the last common bilaterian ancestor. These components, originally directing neuroectodermal patterning and cell migration, served as a gene regulatory 'scaffold' upon which neural crest-like cells with limited migration and potency evolved in the last common ancestor of tunicates and vertebrates. Finally, the acquisition of regulatory programmes controlling multipotency and long-range, directed migration led to the transition from neural crest-like cells in invertebrate chordates to multipotent migratory neural crest in the first vertebrates.
Assuntos
Evolução Biológica , Crista Neural/citologia , Crista Neural/embriologia , Neurogênese , Animais , Diferenciação Celular , Movimento Celular , Invertebrados , VertebradosRESUMO
Neural crest and placodes are key innovations of the vertebrate clade. These cells arise within the dorsal ectoderm of all vertebrate embryos and have the developmental potential to form many of the morphological novelties within the vertebrate head. Each cell population has its own distinct developmental features and generates unique cell types. However, it is essential that neural crest and placodes associate together throughout embryonic development to coordinate the emergence of several features in the head, including almost all of the cranial peripheral sensory nervous system and organs of special sense. Despite the significance of this developmental feat, its evolutionary origins have remained unclear, owing largely to the fact that there has been little comparative (evolutionary) work done on this topic between the jawed vertebrates and cyclostomes-the jawless lampreys and hagfishes. In this review, we briefly summarize the developmental mechanisms and genetics of neural crest and placodes in both jawed and jawless vertebrates. We then discuss recent studies on the role of neural crest and placodes-and their developmental association-in the head of lamprey embryos, and how comparisons with jawed vertebrates can provide insights into the causes and consequences of this event in early vertebrate evolution.
RESUMO
In the zebrafish embryo, expression of the prdm1 and patched1 genes in adaxial cells is indicative of their specification to give rise to slow twitch muscle fibers in response to Hedgehog (Hh) signaling. Subsets of these slow twitch muscle progenitors activate engrailed (eng) strongly in response to high-level Hh signaling, and differentiate into muscle pioneer cells, which are important for subsequent development of the horizontal myoseptum. In addition, eng is expressed more weakly in medial fast fibers in response to lower Hh levels. Somite morphology in the lamprey, an agnathan (jawless) vertebrate, differs significantly from that of teleosts. In particular, the lamprey does not have clear epaxial/hypaxial domains, lacks a horizontal myoseptum, and does not appear to possess distinct populations of fast and slow fibers in the embryonic somite. Nevertheless, Hh is expressed in the midline of the lamprey embryo, and we report here that, as in zebrafish, homologues of patched and prdm1 are expressed in adaxial regions of the lamprey somite, and an eng homologue is also expressed in the somite. However, the lamprey adaxial region does not exhibit the same distinct adaxial cell morphology as in the zebrafish. In addition, the expression of follistatin is not excluded from the adaxial region, and eng is not detected in discrete muscle pioneer-like cells. These data suggest the presence of conserved responses to Hh signaling in lamprey somites, although the full range of effects elicited by Hh in the zebrafish somite is not recapitulated.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento/genética , Genes Controladores do Desenvolvimento/genética , Proteínas Hedgehog/metabolismo , Lampreias/embriologia , Transdução de Sinais/genética , Somitos/metabolismo , Animais , Sequência de Bases , Análise por Conglomerados , DNA Complementar/genética , Hibridização In Situ , Lampreias/metabolismo , Proteínas de Membrana , Dados de Sequência Molecular , Músculo Esquelético/embriologia , Músculo Esquelético/metabolismo , Receptores Patched , Receptor Patched-1 , Filogenia , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Análise de Sequência de DNA , Somitos/anatomia & histologia , Proteínas de Peixe-ZebraRESUMO
snail genes mark presumptive mesoderm across bilaterian animals. In gnathostome vertebrates, snail genes are a multimember family that are also markers of premigratory neural crest (pnc) and some postmigratory neural crest derivatives in the pharyngeal arches. Previous studies of nonvertebrate chordates indicate that they have single snail genes that retain ancestral functions in mesoderm development and perhaps in specification of a pnc-like cell population. Lampreys are the most basal extant vertebrates, with well-defined developmental morphology. Here, we identify a single snail gene from the lamprey Petromyzon marinus that is the phylogenetic outgroup of all gnathostome snail genes. This single lamprey snail gene retains ancestral snail patterning domains present in nonvertebrate chordates. Lamprey snail is also expressed in tissues that are broadly equivalent to the combined sites of expression of all three gnathostome snail paralogy groups, excepting in embryonic tissues that are unique to gnathostomes. Importantly, while snail does not appear to demarcate an early neural crest population in lampreys as it does in gnathostomes, it may be involved in later neural crest development. Together, our results indicate that significant cis-regulatory innovation occurred in a single snail gene before the vertebrate radiation, and significant subfunctionalization occurred after snail gene duplications in the gnathostome lineages.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Lampreias/embriologia , Fatores de Transcrição/metabolismo , Animais , Padronização Corporal , Evolução Molecular , Duplicação Gênica , Crista Neural , Filogenia , Fatores de Transcrição da Família SnailRESUMO
Vertebrates are defined by the presence of the neural crest. These cells contribute to the increased complexity of vertebrates relative to non-vertebrate chordates. It is widely accepted that an increase in vertebrate complexity is also related to gene duplications that occurred at the base of vertebrates and may be related to the origin of the neural crest. Study of the development of one neural crest derivative, pharyngeal cartilage, in the lamprey and comparison to chondrogenesis in other chordates may provide clues regarding acquisition of a chondrogenic fate by the neural crest. The transcription factor Sox9 is thr product of a SoxE gene (Col2a1) that regulates expression of Type II collagenin development of vertebrate cartilage. Duplication of the ancestral SoxE transcription factor into the three SoxE genes present in vertebrates might have been important in partitioning the chondrogenic neural crest role of Sox9. In this review, I discuss evidence that duplicated SoxE genes might have been key in the evolution of chondrogenic neural crest. The recent identification of duplicated SoxE genes, and identification of two Type II collagen genes in lamprey, coupled with their expression in branchial arches suggested ancient regulation of chondrogenesis by a SoxE gene. Examination of SoxE and Type II collagen expression in the developing lamprey branchial arches shows SoxE genes are expressed in developing branchial arch cartilage and Col2a1 genes are expressed in surrounding mesenchymal cells. Lack of cellular co-expression of SoxE genes with Col2a1 suggests additional steps might have been required for direct regulation of Col2a1 by Sox9 in jawed vertebrates.
Assuntos
Evolução Biológica , Colágeno Tipo II/metabolismo , Lampreias/genética , Lampreias/metabolismo , Fatores de Transcrição SOXE/metabolismo , Animais , Colágeno Tipo II/genética , Duplicação Gênica , Regulação da Expressão Gênica , Fatores de Transcrição SOXE/genéticaRESUMO
The proceedings in this volume follow from the 15(th) Center for Developmental Biology meeting on "Advances in Cyclostome Research" that we organized. The meeting was held at the CDB RIKEN Kobe Institute on 24 and 25 January 2008 with support from the CDB. Jawless vertebrates have been of interest to embryologists and comparative morphologists for more than a century. While the comparative morphology among lampreys, hagfishes, and gnathostomes has long been recognized in contributing to understanding the origin of jaws and other gnathostome traits, the availability of modern molecular methods has rekindled interest in these topics, and evolutionary developmental biology coupled with paleontology has opened new avenues into the study of gnathostome origins. Within the last decade, because of renewed interest in evolutionary developmental biology, the importance of lampreys and hagfishes to our understanding of vertebrate evolution has undergone resurgence in interest, as evidenced by the sea lamprey genome project currently underway at the National Human Genome Research Institute. As new molecular and imaging techniques become available, both paleontological and neontological questions are being readdressed and are providing new insights and speculation into vertebrate evolution. Thus, we determined the timing was optimal to bring together many of the researchers currently contributing to our understanding of the biology of agnathans. The diversity of speakers at the meeting included evolutionary developmental biologists, phylogenetics and genomics investigators, paleontologists, and endocrinology researchers, because as we move into the 21(st) century, integration among these disciplines has encouraged synergistic activities to develop. The goal of this meeting was to highlight in a single setting the most recent advances in this important basal group of vertebrates to facilitate interactions among the cyclostome community. Secondarily, we also hope that this gathering will enhance the visibility of jawless vertebrates as important models in the vertebrate "evo-devo" community. Several topics for further discussion emerged at this symposium, including: genomic data that have spurred renewed interest in gene duplications and their contribution to our understanding of cyclostome phylogeny and vertebrate evolution; the use of paleontology coupled with modern imaging techniques to clarify vertebrate phylogeny; and the evolution of the neuroendocrine and adaptive immune systems. These were among the topics that led to fruitful discussion. Here we summarize key research topics from the symposium that continue to advance as we move forward in the 21(st) century.
Assuntos
Evolução Biológica , Feiticeiras (Peixe)/genética , Lampreias/genética , Animais , Cartilagem , Genômica , Arcada Osseodentária , EsqueletoRESUMO
In situ hybridization techniques typically employ chromogenic staining by enzymatic amplification to detect domains of gene expression. We demonstrate the previously unreported near infrared (NIR) fluorescence of the dark purple stain formed from the commonly used chromogens, nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). The solid reaction product has significant fluorescence that enables the use of confocal microscopy to generate high-resolution three-dimensional (3-D) imaging of gene expression.
Assuntos
Hibridização in Situ Fluorescente/métodos , Indóis/química , Nitroazul de Tetrazólio/química , Coloração e Rotulagem , Animais , Miosinas Cardíacas/genética , Miosinas Cardíacas/metabolismo , Embrião não Mamífero , Lampreias/embriologia , Microscopia Confocal , Peixe-Zebra/embriologiaRESUMO
Ectodermal placodes contribute to the cranial ganglia and sense organs of the head and, together with neural crest cells, represent defining features of the vertebrate embryo. The identity of different placodes appears to be specified in part by the expression of different Pax genes, with Pax-3/7 class genes being expressed in the trigeminal placode of mice, chick, frogs and fish, and Pax-2/5/8 class genes expressed in the otic placode. Here, we present the cloning and expression pattern of lamprey Pax-7 and Pax-2, which mark the trigeminal and otic placodes, respectively, as well as other structures characteristic of vertebrate Pax genes. These results suggest conservation of Pax genes and placodal structures in basal and derived vertebrates.