RESUMO
Despite the suboptimal aspects of domestic breeding conditions compared with the natural conditions under which their reproductive behavior evolved, most domestic stallions can adapt to management and breeding programs. Most respond adequately or quickly learn to safely abide the restraint and direction of a human handler, and can adapt to changes in methods of breeding for semen collection. If not, the problems can range from inadequate or variable sexual interest and response to overenthusiastic or aggressive response beyond the ability of the handlers to safely direct and control. This article discusses veterinary evaluation as well as housing and handling strategies for addressing stallion breeding behavior problems.
Assuntos
Criação de Animais Domésticos , Cruzamento , Doenças dos Cavalos/diagnóstico , Transtornos Mentais/veterinária , Animais , Feminino , Doenças dos Cavalos/terapia , Cavalos , Masculino , Transtornos Mentais/diagnóstico , Transtornos Mentais/terapiaRESUMO
OBJECTIVE: To describe a standing hand-assisted laparoscopic ovariohysterectomy in a mare. ANIMAL: A 15-year-old maiden Oldenburg mare. CLINICAL PRESENTATION, PROGRESSION, AND PROCEDURES: The mare was presented for evaluation of bucking under saddle and uncharacteristic aggressive behavior. Evaluation of a 24-hour video of the mare in a stall showed behavior consistent with caudal visceral abdominal discomfort. Reproductive evaluation revealed a pyometra secondary to complete transluminal cervical adhesions. The mare was initially managed medically with disruption of the adhesions and uterine lavage, but the adhesions reformed within 6 weeks and could not be manually disrupted. TREATMENT AND OUTCOME: To eliminate the recurrence of pyometra, the mare underwent standing hand-assisted laparoscopic ovariohysterectomy through bilateral flank incisions. The only complication was a seroma at 1 flank incision that resolved after drainage. CLINICAL RELEVANCE: Complete ovariohysterectomy in the mare is a challenging procedure and has previously been performed under general anesthesia. This is the first report of the procedure being performed completely in the standing mare without inversion of the uterus through the cervix.
Assuntos
Laparoscopia Assistida com a Mão , Doenças dos Cavalos , Piometra , Cavalos , Feminino , Animais , Piometra/cirurgia , Piometra/veterinária , Laparoscopia Assistida com a Mão/efeitos adversos , Laparoscopia Assistida com a Mão/veterinária , Histerectomia/veterinária , Histerectomia/métodos , Ovariectomia/veterinária , Ovariectomia/métodos , Reprodução , Doenças dos Cavalos/cirurgiaRESUMO
Pairing of the sex chromosomes during mammalian meiosis is characterized by the formation of a unique heterochromatin structure at the XY body. The mechanisms underlying the formation of this nuclear domain are reportedly highly conserved from marsupials to mammals. In this study, we demonstrate that in contrast to all eutherian species studied to date, partial synapsis of the heterologous sex chromosomes during pachytene stage in the horse is not associated with the formation of a typical macrochromatin domain at the XY body. While phosphorylated histone H2AX (γH2AX) and macroH2A1.2 are present as a diffuse signal over the entire macrochromatin domain in mouse pachytene spermatocytes, γH2AX, macroH2A1.2, and the cohesin subunit SMC3 are preferentially enriched at meiotic sex chromosome cores in equine spermatocytes. Moreover, although several histone modifications associated with this nuclear domain in the mouse such as H3K4me2 and ubH2A are conspicuously absent in the equine XY body, prominent RNA polymerase II foci persist at the sex chromosomes. Thus, the localization of key marker proteins and histone modifications associated with the XY body in the horse differs significantly from all other mammalian systems described. These results demonstrate that the epigenetic landscape and heterochromatinization of the equine XY body might be regulated by alternative mechanisms and that some features of XY body formation may be evolutionary divergent in the domestic horse. We propose equine spermatogenesis as a unique model system for the study of the regulatory networks leading to the epigenetic control of gene expression during XY body formation.
Assuntos
Cromatina/química , Pareamento Cromossômico , Cavalos/genética , Cromossomos Sexuais/genética , Espermatogênese/genética , Animais , Proteínas Cromossômicas não Histona/metabolismo , Epigenômica , Heterocromatina/genética , Histonas/genética , Camundongos , Miose/genética , Estágio Paquíteno/genéticaRESUMO
CASE DESCRIPTION: 2 Standardbred racehorses that had been winning races while competing as mares underwent postrace drug testing and had serum testosterone concentrations above the acceptable limit for female racehorses. CLINICAL FINDINGS: Initial physical examinations by the referring veterinarian revealed ambiguous external genitalia and suspected intra-abdominally located testes leading to a preliminary diagnosis of male pseudohermaphroditism. Horses were referred for further evaluation of sex. Physical examination of the external genitalia confirmed the findings of the referring veterinarian. Transrectal palpation and ultrasonography revealed gonads with an ultrasonographic appearance of testes. On cytogenetic analysis, both horses were determined to have a 64,XY karyotype and 8 intact Y chromosome markers and 5 SRY gene markers, which were indicative of a genetic male and confirmed an intersex condition. Additionally, both horses had some male-type behavior and endocrinologic findings consistent with those of sexually intact males. TREATMENT AND OUTCOME: Taken together, these findings confirmed that both horses were male pseudohermaphrodites. Both horses returned to racing competition as males. CLINICAL RELEVANCE: As of October 1, 2008, the Pennsylvania Horse and Harness Racing Commissions implemented a postrace drug testing policy that included analysis of blood samples for anabolic and androgenic steroids and set maximum allowable concentrations of testosterone for racing geldings and females. Within 8 months of initiation of this drug testing policy, the 2 horses of this report were identified as having an intersex condition. This raises the possibility that intersex conditions may be more common in racing Standardbreds than was previously suspected.
Assuntos
Transtorno 46,XY do Desenvolvimento Sexual/veterinária , Cavalos/genética , Cavalos/fisiologia , Cariotipagem , Testosterona/sangue , Animais , Comportamento Animal , Transtorno 46,XY do Desenvolvimento Sexual/diagnóstico , Feminino , Masculino , EsportesRESUMO
Proper pain therapy requires adequate pain assessment. This study evaluated the reliability and validity of the Unesp-Botucatu horse acute pain scale (UHAPS), the Orthopedic Composite Pain Scale (CPS) and unidimensional scales in horses admitted for orthopedic and soft tissue surgery. Forty-two horses were assessed and videotaped before surgery, up to 4 hours postoperatively, up to 3 hours after analgesic treatment, and 24 hours postoperatively (168 video clips). After six evaluators viewing each edited video clip twice in random order at a 20-day interval, they chose whether analgesia would be indicated and applied the Simple Descriptive, Numeric and Visual Analog scales, CPS, and UHAPS. For all evaluators, intra-observer reliability of UHAPS and CPS ranged from 0.70 to 0.97. Reproducibility was variable among the evaluators and ranged from poor to very good for all scales. Principal component analysis showed a weak association among 50% and 62% of the UHAPS and CPS items, respectively. Criterion validity based on Spearman correlation among all scales was above 0.67. Internal consistency was minimally acceptable (0.51-0.64). Item-total correlation was acceptable (0.3-0.7) for 50% and 38% of UHAPS and CPS items, respectively. UHAPS and CPS were specific (90% and 79% respectively), but both were not sensitive (43 and 38%, respectively). Construct validity (responsiveness) was confirmed for all scales because pain scores increased after surgery. The cut-off point for rescue analgesia was ≥ 5 and ≥ 7 for the UHAPS and CPS, respectively. All scales presented adequate repeatability, criterion validity, and partial responsiveness. Both composite scales showed poor association among items, minimally acceptable internal consistency, and weak sensitivity, indicating that they are suboptimal instruments for assessing postoperative pain. Both composite scales require further refinement with the exclusion of redundant or needless items and reduction of their maximum score applied to each item or should be replaced by other tools.
Assuntos
Doenças dos Cavalos/diagnóstico , Manejo da Dor/veterinária , Medição da Dor/veterinária , Dor Pós-Operatória/veterinária , Animais , Feminino , Doenças dos Cavalos/cirurgia , Cavalos , Masculino , Manejo da Dor/métodos , Medição da Dor/métodos , Dor Pós-Operatória/diagnóstico , Dor Pós-Operatória/cirurgiaRESUMO
BACKGROUND: Early development of the gut microbiome is an essential part of neonate health in animals. It is unclear whether the acquisition of gut microbes is different between domesticated animals and their wild counterparts. In this study, fecal samples from ten domestic conventionally managed (DCM) Standardbred and ten semi-feral managed (SFM) Shetland-type pony foals and dams were compared using 16S rRNA sequencing to identify differences in the development of the foal hindgut microbiome related to time and management. RESULTS: Gut microbiome diversity of dams was lower than foals overall and within groups, and foals from both groups at Week 1 had less diverse gut microbiomes than subsequent weeks. The core microbiomes of SFM dams and foals had more taxa overall, and greater numbers of taxa within species groups when compared to DCM dams and foals. The gut microbiomes of SFM foals demonstrated enhanced diversity of key groups: Verrucomicrobia (RFP12), Ruminococcaceae, Fusobacterium spp., and Bacteroides spp., based on age and management. Lactic acid bacteria Lactobacillus spp. and other Lactobacillaceae genera were enriched only in DCM foals, specifically during their second and third week of life. Predicted microbiome functions estimated computationally suggested that SFM foals had higher mean sequence counts for taxa contributing to the digestion of lipids, simple and complex carbohydrates, and protein. DCM foal microbiomes were more similar to their dams in week five and six than were SFM foals at the same age. CONCLUSIONS: This study demonstrates the impact of management on the development of the foal gut microbiome in the first 6 weeks of life. The higher numbers of taxa within and between bacterial groups found in SFM dams and foals suggests more diversity and functional redundancy in their gut microbiomes, which could lend greater stability and resiliency to these communities. The colonization of lactic acid bacteria in the early life of DCM foals suggests enrichment in response to the availability of dams' feed. Thus, management type is an important driver of gut microbiome establishment on horses, and we may look to semi-feral horses for guidance in defining a healthy gut microbiome for domestic horses.
RESUMO
Infanticide, related to a stallion's aggression toward a foal sired by another stallion, and feticide related to a new stallion's aggression and/or pheromonal influence (the Bruce effect) inducing loss of a fetus sired by another stallion, a female's counteraction to infanticide, have been proposed for domestic horses (Equus caballus) in human-managed conditions. The aim of the present study was, in conditions close to natural, to investigate the influence of the natural succession of a harem stallion on the mares' subsequent reproductive performance. In a population of semi-feral Konik polski horses observed for 31 years (reproductive seasons) in 8 bands, harem stallion changed 10 times. These changes involved 26 out of 48 mares and 60 out of 609 observed mare-seasons (MS, a year in which a mare experienced a reproductive event). Binary distribution and log link function were assumed. The marginal model included the classification variable (SCH) and the continuous variables (age of the mare and calendar year of reproductive event (birth of a live foal, abortion, foals lost or barrenness) in a given MS was analyzed with generalized linear mixed model. The reproductive fitness of mares and their reproductive success (foal surviving ≥ 1 year), did not differ between MS with and without SCH. Older females were more likely to stay barren, with chances increasing by 21% with each successive year; and less likely to give birth to a foal (13% decrease of chance), and rear a foal to one year of age (12% decrease of chance). The age did not affect the probability of abortions. Of the 26 MS when mares were pregnant when the stallion had changed, there were 25 healthy foals born. For the entire 31 years of monitoring, no aggression toward any foal was observed and all foals that were born in the harem of a new, succeeding stallion successfully reached adulthood. Due to the lack of incidents of infanticide and the lack of evidence suggesting that the presence of a new harem stallion leads to the termination of pregnancies sired by another stallion, the Bruce effect was not confirmed as a biological strategy to reduce investment in pregnancy and potential infanticide in studied population of semi-feral horses.
Assuntos
Criação de Animais Domésticos , Cavalos/fisiologia , Infertilidade Feminina , Envelhecimento , Animais , Feminino , Masculino , Gravidez , Comportamento Sexual AnimalRESUMO
Mushroom is a unique coat color phenotype in Shetland Ponies characterized by the dilution of the chestnut coat color to a sepia tone and is hypothesized to be a recessive trait. A genome wide association study (GWAS), utilizing the Affymetrix 670K array (MNEc670k) and a single locus mixed linear model analysis (EMMAX), identified a locus on ECA7 for further investigation (Pcorrected = 2.08 × 10-10). This locus contained a 3 Mb run of homozygosity in the 12 mushroom ponies tested. Analysis of high throughput Illumina sequencing data from one mushroom Shetland pony compared to 87 genomes from horses of various breeds, uncovered a frameshift variant, p.Asp201fs, in the MFSD12 gene encoding the major facilitator superfamily domain containing 12 protein. This variant was perfectly concordant with phenotype in 96 Shetland Ponies (P = 1.15 × 10-22), was identified in the closely related Miniature Horse for which the mushroom phenotype is suspected to occur (fmu = 0.02), and was absent in 252 individuals from seven additional breeds not reported to have the mushroom phenotype. MFSD12 is highly expressed in melanocytes and variants in this gene in humans, mice, and dogs impact pigmentation. Given the role of MFSD12 in melanogenesis, we propose that p.Asp201fs is causal for the dilution observed in mushroom ponies.
Assuntos
Mutação da Fase de Leitura , Cavalos/genética , Pigmentação/genética , Pelo Animal/metabolismo , Animais , Proteínas de Membrana Transportadoras/genéticaRESUMO
Self-mutilation in horses includes biting, stomping and kicking, rubbing, and lunging into objects. Based on the author's clinical experience, three distinct types of self-mutilation are proposed and described. Type I represents normal behavioral response to continuous or intermittent physical discomfort. Type II, seen in stallions and geldings, can be recognized as self-directed intermale aggression. The behavior includes the elements and order of the natural interactive sequence typical of encounters between two stallions, except that the stallion himself is the target of his intermale behavior. Type III involves a more quiet, often rhythmically repetitive or methodical behavioral sequence of a stereotypy, for example nipping at various areas of the body in a relatively invariant pattern, stomping, or kicking rhythmically against an object. The prevalence of the various types of self-mutilation in horses is not known. In one survey, results suggested that self-mutilation of one form or another has been observed in as many as 2% of domestic stallions. Among and between stallions, self-mutilation varies in frequency and intensity, and can reach levels that are fertility and/or life threatening. Careful evaluation of the horse's behavior is often necessary to distinguish the specific type. Type I self-mutilation, where physical discomfort is the root cause, can be eliminated by relieving the discomfort. For Types II and III, understanding of intermale interactive behavior of horses and the environmental factors that may trigger or exacerbate the self-mutilative form, can be useful in guiding humane management or behavior modification. Pharmacologic interventions may be a useful adjunct to management and nutritional changes.
Assuntos
Doenças dos Cavalos/etiologia , Cavalos/fisiologia , Automutilação/etiologia , Agressão/fisiologia , Animais , Doenças dos Cavalos/epidemiologia , Doenças dos Cavalos/terapia , Masculino , Automutilação/epidemiologia , Automutilação/terapiaRESUMO
Horses naturally exposed to West Nile Virus (WNV) or vaccinated against WNV develop humoral immunity thought to be protective against development of clinical disease in exposed or infected animals. No reports evaluate the efficacy of passive transfer of naturally acquired specific WNV humoral immunity from dam to foal. The purpose of this study was to investigate passive transfer of naturally acquired immunity to WNV to foals born in a herd of semi-feral ponies, not vaccinated against WNV, in an endemic area, with many dams having seroconverted because of natural exposure. Microwell serum neutralization titers against WNV were determined in all mares and foals. Serum IgG concentration was determined in foals by serial radial immunodiffusion. Differences in IgG concentration between seropositive and seronegative foals were examined by means of the Mann-Whitney U-test. Linear regression was used to evaluate the association between mare and foal titers. Seventeen mare-foal pairs were studied; 1 foal had inadequate IgG concentration. IgG concentration was not different between seronegative and seropositive foals (P = .24). Mare and foal titers were significantly correlated in foals with adequate passive transfer of immunity (Spearman's rho = .84; P < .001); >90% of the foal's titer was explained by the mare's titer (R2 = 0.91; P < .001). Passive transfer of specific immunity to WNV is present in pony foals with adequate passive transfer of immunity born to seroconverted mares.
Assuntos
Doenças dos Cavalos/imunologia , Imunidade Materno-Adquirida/imunologia , Febre do Nilo Ocidental/veterinária , Vírus do Nilo Ocidental/imunologia , Animais , Cavalos , Febre do Nilo Ocidental/imunologiaRESUMO
Periodic spontaneous erection and penile movements known as masturbation (SEAM) occur normally at approximately 90 min intervals in awake equids. SEAM in horses has traditionally been misunderstood by many horsemen as aberrant behavior that should be eliminated. Accordingly, it is not uncommon for trainers of performance stallions or managers of breeding stallions to punish SEAM in an attempt to eliminate the behavior. Previous clinical observations and preliminary unsystematic trials had suggested that attempts to stop stallion SEAM may lead to an increase rather than a decrease in SEAM, and at the same time may suppress sexual behavior (SB) in a breeding stallion. The present work evaluated the effects of aversive conditioning of SEAM on SEAM, SB, and semen. In Experiment 1, four mature pony stallions were subjected to aversive conditioning of SEAM in a within- and between-subjects half cross-over design. The SEAM erection interval tended to be less after aversive conditioning, suggesting an increase in SEAM frequency. Eleven other SEAM measures were each similar before and after aversive conditioning of SEAM. In standard sexual behavior trials with a stimulus mare and dummy mount, erection latency, ejaculation latency, mount readiness latency, and number of mounts to ejaculation increased after aversive conditioning of SEAM; erection rigidity score, number of ejaculatory pulses, and vocalization rate decreased. Number of thrusts to ejaculation was similar before and after aversive conditioning of SEAM. All affected SB measures indicated suppressed sexual arousal and breeding efficiency after SEAM. In Experiment 1, ejaculated semen was not evaluated. Because in Experiment 1, the number of ejaculatory urethral pulses was less after aversive conditioning, Experiment 2 was similarly designed, but included evaluation of semen, both immediately and again 1 week after aversive conditioning was completed. Experiment 2 included 12 aversively conditioned stallions, and 4 yoked controls. In Experiment 2, masturbation episode duration tended to be less after aversive conditioning, while the remaining 11 SEAM measures were unaffected by aversive conditioning of SEAM. Of SB measures, erection latency, mount readiness latency, thrusts to ejaculation, and ejaculation latency were significantly greater after aversive conditioning. Erection rigidity score and number of ejaculatory pulses were less after aversive conditioning. These differences are consistent with suppressed sexual arousal and reduced breeding efficiency. Semen volume and total number of sperm per ejaculate were significantly less after aversive conditioning. These findings are consistent with clinical anecdotes and preliminary trials indicating that aversive conditioning of SEAM in stallions suppresses sexual arousal and breeding behavior. Of considerable interest both clinically and theoretically, is the finding that aversive conditioning target behavior of SEAM was not suppressed by aversive conditioning, while SB and semen during semen collection trials were both adversely affected.
Assuntos
Condicionamento Psicológico , Cavalos/fisiologia , Ereção Peniana/fisiologia , Sêmen/fisiologia , Comportamento Sexual Animal/fisiologia , Animais , Terapia Aversiva , Cruzamento , Ejaculação , Eletrochoque , Masculino , MasturbaçãoRESUMO
The purpose of this study was to evaluate the variation in plasma adrenocorticotropic hormone (ACTH) concentration and dexamethasone suppression test (DST) results with season, age, and sex in healthy, pony mares (n=15) and pony stallions (n=14) living under semiferal conditions and horse mares (n=10) living at pasture. Plasma ACTH concentrations were measured in September 2002, and in January, May, and September 2003. DSTs were performed in January and September 2003. Plasma ACTH concentrations in September 2002 and September 2003 were similar and were significantly greater than in January and May (P < .001). Plasma ACTH concentration was within the reference range for 38 (97%) of 39 subjects in January, for 39 (100%) of 39 subjects in May, for 2 (5%) of 39 subjects in September 2002, and for 3 (8%) of 39 subjects in September 2003. DST results were within the reference range in all subjects in January and were within the reference range for 29 (74%) of 39 subjects in September 2003. Plasma cortisol concentration at the end of the DST was significantly greater in September than in January (P = .002). Age was positively correlated with plasma ACTH and plasma cortisol concentration at the beginning and end of the DST Within the same season, plasma ACTH concentration in pony mares, pony stallions, and horse mares was not significantly different (P > .05). Seasonal changes in plasma ACTH concentration and DST results should be considered when interpreting endocrine test results.
Assuntos
Hormônio Adrenocorticotrópico/sangue , Dexametasona , Cavalos/sangue , Envelhecimento/sangue , Animais , Tamanho Corporal , Síndrome de Cushing/diagnóstico , Síndrome de Cushing/veterinária , Feminino , Doenças dos Cavalos/diagnóstico , Masculino , Estações do Ano , Fatores SexuaisRESUMO
OBJECTIVE: To evaluate behavioral compliance of horses and ponies with simulated intranasal vaccination and assess development of generalized aversion to veterinary manipulations. DESIGN: Clinical trial. ANIMALS: 28 light horse mares, 3 pony geldings, 2 light horse stallions, and 3 pony stallions that had a history of compliance with veterinary procedures. PROCEDURE: Behavioral compliance with 2 intranasal vaccine applicators was assessed. Compliance with standard physical examination procedures was assessed before and after a single experience with either of the applicators or a control manipulation to evaluate development of generalized aversion to veterinary manipulation. RESULTS: In all 30 horses, simulated intranasal vaccination or the control manipulation could be performed without problematic avoidance behavior, and simulated intranasal vaccination did not have any significant effect on duration of or compliance with a standardized physical examination that included manipulation of the ears, nose, and mouth. Results were similar for the 2 intranasal vaccine applicators, and no difference in compliance was seen between horses in which warm versus cold applicators were used. For 3 of the 6 ponies, substantial avoidance behavior was observed in association with simulated intranasal vaccination, and compliance with physical examination procedures decreased after simulated intranasal vaccination. CONCLUSIONS AND CLINICAL RELEVANCE: Although some compliance problems were seen with ponies, neither problems with compliance with simulated intranasal vaccination nor adverse effects on subsequent physical examination were identified in any of the horses. Further study is needed to understand factors involved in practitioner reports of aversion developing in association with intranasal vaccination.
Assuntos
Comportamento Animal , Cavalos/psicologia , Imunização/veterinária , Exame Físico/veterinária , Vacinas/administração & dosagem , Administração Intranasal , Animais , Feminino , Cavalos/fisiologia , Imunização/instrumentação , Imunização/métodos , Masculino , Cooperação do Paciente/psicologia , Exame Físico/psicologia , Gravação de VideoteipeRESUMO
OBJECTIVE: To investigate effects of sample handling, storage, and collection time and season on plasma alpha-melanocyte-stimulating hormone (alpha-MSH) concentration in healthy equids. ANIMALS: 11 healthy Standardbreds and 13 healthy semiferal ponies. PROCEDURE: Plasma alpha-MSH concentration was measured by use of radioimmunoassay. Effects of delayed processing were accessed by comparing alpha-MSH concentrations in plasma immediately separated with that of plasma obtained from blood samples that were stored at 4 degrees C for 8 or 48 hours before plasma was separated. Effects of suboptimal handling were accessed by comparing alpha-MSH concentrations in plasma immediately stored at -80 degrees C with plasma that was stored at 25 degrees C for 24 hours, 4 degrees C for 48 hours or 7 days, and -20 degrees C for 30 days prior to freezing at -80 degrees C. Plasma alpha-MSH concentrations were compared among blood samples collected at 8:00 AM, 12 noon, and 4:00 PM. Plasma alpha-MSH concentrations were compared among blood samples collected in January, March, April, June, September, and November from horses and in September and May from ponies. RESULTS: Storage of blood samples at 4 degrees C for 48 hours before plasma was separated and storage of plasma samples at 4 degrees C for 7 days prior to freezing at -80 degrees C resulted in significant decreases in plasma alpha-MSH concentrations. A significantly greater plasma alpha-MSH concentration was found in September in ponies (11-fold) and horses (2-fold), compared with plasma alpha-MSH concentrations in spring. CONCLUSIONS AND CLINICAL RELEVANCE: Handling and storage conditions minimally affected plasma alpha-MSH concentrations. Seasonal variation in plasma alpha-MSH concentrations must be considered when evaluating pituitary pars intermedia dysfunction in equids.
Assuntos
Cavalos/sangue , Estações do Ano , alfa-MSH/sangue , Análise de Variância , Animais , Radioimunoensaio , Manejo de Espécimes , Fatores de TempoRESUMO
The objective of this study was to determine whether an indenopyridine derivative RTI-4587-073(l) was a good candidate for male contraception in horses. We hypothesized that a single administration of RTI-4587-073(l) causes significant suppression of testicular function in stallions without affecting sexual behavior. Three Miniature horse stallions received a single dose of 12.5 mg/kg RTI-4587-073(l) orally (group "treated"), whereas three other Miniature horse stallions received placebo only (group "control"). Semen was collected and evaluated from all stallions twice a week for three baseline weeks and 13 post-treatment weeks. Sexual behavior was video-recorded and analyzed. Testicular dimensions were measured using ultrasonography, and blood samples were drawn for endocrine evaluation once before treatment and once a week during the post-treatment period. Single administration of RTI-4587-073(l) caused severe oligoasthenozoospermia (low sperm number and low motility), shedding large numbers of immature germ cells in semen, and increased FSH concentrations in treated stallions. These effects were fully reversible within â¼71 days. However, libido and copulatory behavior remained unchanged throughout the entire experiment. We concluded that RTI-4587-073(l) was a promising candidate for male contraceptive in domestic stallions. Further research should be performed to test this compound for fertility control in wildlife and humans.
Assuntos
Anticoncepção/veterinária , Anticoncepcionais Masculinos/farmacologia , Cavalos/fisiologia , Indenos/farmacologia , Piperidinas/farmacologia , Animais , Anticoncepção/métodos , Estradiol/sangue , Hormônio Foliculoestimulante/sangue , Inibinas/sangue , Hormônio Luteinizante/sangue , Masculino , Análise do Sêmen , Comportamento Sexual Animal/efeitos dos fármacos , Testículo/efeitos dos fármacos , Testosterona/sangueAssuntos
Cruzamento/métodos , Fertilidade/fisiologia , Cavalos/fisiologia , Reprodução/fisiologia , Esportes , Animais , Feminino , Masculino , Linhagem , Gravidez , Resultado da Gravidez , Taxa de GravidezAssuntos
Doenças dos Cavalos/diagnóstico por imagem , Cavalos/fisiologia , Cálculos Renais/veterinária , Contagem de Espermatozoides/veterinária , Motilidade dos Espermatozoides , Animais , Comportamento Animal , Doenças dos Cavalos/fisiopatologia , Doenças dos Cavalos/cirurgia , Cálculos Renais/diagnóstico por imagem , Cálculos Renais/fisiopatologia , Cálculos Renais/cirurgia , Masculino , Resultado do Tratamento , UltrassonografiaRESUMO
Enalapril is an angiotensin converting enzyme (ACE) inhibitor that is frequently used in human, feline and canine patients with cardiac disease. Its use has been associated with impotence in human patients. The purpose of this study was to evaluate if enalapril (0.5mg/kg PO, q24h) is likely to alter behavior in stallions and to assess its effect on ACE activity at the standard dose used in dogs and cats. Twelve pony stallions were evaluated by physical examination and echocardiography followed by treatment with enalapril (n=6) or placebo (n=6) for 2 months. After one month, blood was drawn and stored to evaluate ACE activity in the 2 groups. At the end of the study, repeat physical examination and echocardiography were performed. Physical examination, echocardiographic indices, and reproductive performance were unchanged and there was no suppression of ACE activity. Results of this study suggest that enalapril (0.5mg/kg PO, q24h) is either poorly absorbed in the horse or is inadequately converted to the active form of the drug, enalaprilat.