[Multifactorial analysis of the risk factors of chronic prostatitis].

OBJECTIVE: To investigate the risk factors of chronic prostatitis. METHODS: An epidemiological survey of 1,168 patients with chronic prostatitis was conducted and both single factor and multifactorial logistic regression analyses were performed. RESULTS AND CONCLUSION: Many risk factors were identified, including urethritis, uncontrolled sexual activity, frequent masturbation, urinary system injury, fixed body posture (especially long-time riding), excessive drinking, long-time holding urine. The findings may provide reference for the prevention, treatment and measures for coping with recurrence of chronic prostatitis.

[Preparation and functional identification of testicular Sertoli cells].

OBJECTIVE: To simplify the method for separation and cultivation of rat testicular Sertoli cells with high viability, quantity and expression efficiency. METHODS: Testicular Sertoli cells from 2 to 3-week-old male Wistar rats were prepared by digestion with collagenase, trypsin and DNase and cultured together with active lymphocytes to observe their killing effect against lymphocytes. After cell culture for 72 h, the Sertoli cells were morphologically observed by different means and identified with transmission electron microscope. Fas ligand and follicle-stimulating hormone receptor (FSHR) were examined immunohistochemically to identify testicular Sertoli cells. SABC method was used for labeling the Fas ligand on the testicular Sertoli cells. RESULTS: The viability of the isolated and cultured Sertoli cells was more than 90%, and in in vitro culture, Sertoli cells, which expressed the Fas ligand, could kill the active lymphocytes. CONCLUSION: This method improves the efficiency in acquisition of rat testicular Sertoli cells expressing Fas ligand, which are believed to be a potential donor for co-transplantation with parathyroid cells to offer immune privilege.