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1.
Dokl Biochem Biophys ; 481(1): 190-194, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30168056

RESUMO

The PepP protein has been purified in vitro and characterized for the first time. It is encoded by the sll0136 gene of the unicellular cyanobacterium Synechocystis sp. PCC6803. It is established that the PepP protein is a Mn2+-dependent Xaa-Pro-specific aminopeptidase. The protein in the reaction of hydrolysis of the fluorescent peptide Lys(N-Abz)-Pro-Pro-pNA has a maximal activity at pH 7.6 and 32°C.


Assuntos
Aminopeptidases/química , Aminopeptidases/metabolismo , Synechocystis/enzimologia , Hidrólise , Cinética , Metais/farmacologia , Modelos Moleculares , Conformação Proteica , Especificidade por Substrato
2.
Mol Biol (Mosk) ; 46(1): 147-55, 2012.
Artigo em Russo | MEDLINE | ID: mdl-22642112

RESUMO

Prokaryotic cells, including cyanobacteria, respond to a decrease in ambient temperature by activation of numerous cold shock genes. Low temperatures cause a decrease in membrane fluidity, which is maintained at some optimal level mainly by fatty acid (FA) desaturases. Here, temperature-dependent expression of the desB gene for the omega3-desaturase in Synechocystis, which synthesized polyunsaturated FAs, and in its mutant, desA-/desD-, which is defective in genes for delta12- and delta6-desaturases and is capable of synthesizing only monounsaturated FAs was studied. Low temperatures caused the increase in the amount of the desB mRNA in the wild-type cells with the maximum observed at 24 degrees C. In the double mutant desA-/desD- cells, the maximum amount of this mRNA was accumulated at 28-30 degrees C. Thus, our studies of the desB gene for the omega3-desaturase demonstrated that temperature-dependent expression of genes, which are responsible for the maintenance of the optimal membrane fluidity, depends on physical state of these membranes and is regulated by a feedback mode.


Assuntos
Cianobactérias/genética , Cianobactérias/metabolismo , Ácidos Graxos Dessaturases/genética , Ácidos Graxos Dessaturases/metabolismo , Cianobactérias/fisiologia , Ácidos Graxos Dessaturases/isolamento & purificação , Ácidos Graxos Ômega-3/biossíntese , Ácidos Graxos Ômega-3/genética , Retroalimentação Fisiológica , Regulação da Expressão Gênica/fisiologia , Fluidez de Membrana/genética , Fluidez de Membrana/fisiologia , Mutação , Temperatura
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