RESUMO
Collective cell migration is a key process during the development of multicellular organisms, in which the migrations of individual cells are coordinated through chemical guidance and physical contact between cells. Talin has been implicated in mechanical linkage between actin-based motile machinery and adhesion molecules, but how talin contributes to collective cell migration is unclear. Here we show that talin B is involved in chemical coordination between cells for collective cell migration at the multicellular mound stage in the development of Dictyostelium discoideum. From early aggregation to the mound formation, talB-null cells exhibited collective migration normally with cAMP relay. Subsequently, talB-null cells showed developmental arrest at the mound stage, and at the same time, they had impaired collective migration and cAMP relay, while wild-type cells exhibited rotational cell migration continuously in concert with cAMP relay during the mound stage. Genetic suppression of PI3K activity partially restored talB-null phenotypes in collective cell migration and cAMP relay. Overall, our observations suggest that talin B regulates chemical coordination via PI3K-mediated signaling in a stage-specific manner for the multicellular development of Dictyostelium cells.
Assuntos
Movimento Celular , Dictyostelium/citologia , Fosfatidilinositol 3-Quinases/metabolismo , Talina/fisiologia , Agregação Celular , AMP Cíclico/metabolismo , Dictyostelium/metabolismo , Proteínas de ProtozoáriosRESUMO
Chemotactic eukaryote cells can sense chemical gradients over a wide range of concentrations via heterotrimeric G-protein signaling; however, the underlying wide-range sensing mechanisms are only partially understood. Here we report that a novel regulator of G proteins, G protein-interacting protein 1 (Gip1), is essential for extending the chemotactic range ofDictyosteliumcells. Genetic disruption of Gip1 caused severe defects in gradient sensing and directed cell migration at high but not low concentrations of chemoattractant. Also, Gip1 was found to bind and sequester G proteins in cytosolic pools. Receptor activation induced G-protein translocation to the plasma membrane from the cytosol in a Gip1-dependent manner, causing a biased redistribution of G protein on the membrane along a chemoattractant gradient. These findings suggest that Gip1 regulates G-protein shuttling between the cytosol and the membrane to ensure the availability and biased redistribution of G protein on the membrane for receptor-mediated chemotactic signaling. This mechanism offers an explanation for the wide-range sensing seen in eukaryotic chemotaxis.
Assuntos
Membrana Celular/metabolismo , Quimiotaxia/fisiologia , Dictyostelium/metabolismo , Reguladores de Proteínas de Ligação ao GTP/metabolismo , Proteínas Heterotriméricas de Ligação ao GTP/metabolismo , Transdução de Sinais/fisiologia , Membrana Celular/genética , Dictyostelium/genética , Reguladores de Proteínas de Ligação ao GTP/genética , Proteínas Heterotriméricas de Ligação ao GTP/genéticaRESUMO
The wide range sensing of extracellular signals is a common feature of various sensory cells. Eukaryotic chemotactic cells driven by GPCRs and their cognate G proteins are one example. This system endows the cells directional motility towards their destination over long distances. There are several mechanisms to achieve the long dynamic range, including negative regulation of the receptors upon ligand interaction and spatial regulation of G proteins, as we found recently. However, these mechanisms are insufficient to explain the 105-fold range of chemotaxis seen in Dictyostelium. Here, we reveal that the receptor-mediated activation, recruitment, and capturing of G proteins mediate chemotactic signaling at the lower, middle and higher concentration ranges, respectively. These multiple mechanisms of G protein dynamics can successfully cover distinct ranges of ligand concentrations, resulting in seamless and broad chemotaxis. Furthermore, single-molecule imaging analysis showed that the activated Gα subunit forms an unconventional complex with the agonist-bound receptor. This complex formation of GPCR-Gα increased the membrane-binding time of individual Gα molecules and therefore resulted in the local accumulation of Gα. Our findings provide an additional chemotactic dynamic range mechanism in which multiple G protein dynamics positively contribute to the production of gradient information.
Assuntos
Quimiotaxia , Dictyostelium/citologia , Dictyostelium/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , AMP Cíclico/metabolismo , Espaço Intracelular/metabolismo , Proteínas de Protozoários/metabolismo , Transdução de SinaisRESUMO
Integrin LFA-1 regulates immune cell adhesion and trafficking by binding to ICAM-1 upon chemokine stimulation. Integrin-mediated clutch formation between extracellular ICAM-1 and the intracellular actin cytoskeleton is important for cell adhesion. We applied single-molecule tracking analysis to LFA-1 and ICAM-1 in living cells to examine the ligand-binding kinetics and mobility of the molecular clutch under chemokine-induced physiological adhesion and Mn(2+)-induced tight adhesion. Our results show a transient LFA-1-mediated clutch formation that lasts a few seconds and leads to a transient lower-mobility is sufficient to promote cell adhesion. Stable clutch formation was observed for Mn(2+)-induced high affinity LFA-1, but was not required for physiological adhesion. We propose that fast cycling of the clutch formation by intermediate-affinity integrin enables dynamic cell adhesion and migration.
Assuntos
Adesão Celular/fisiologia , Antígeno-1 Associado à Função Linfocitária/fisiologia , Humanos , Molécula 1 de Adesão Intercelular/metabolismo , Cinética , Antígeno-1 Associado à Função Linfocitária/metabolismoRESUMO
To detect single molecules within the optical diffraction limit (< ca. 200 nm), a multicolored imaging technique is developed using Halo-ligand conjugated quantum dots (Halo-QDs; <6 nm in diameter). Using three types of Halo-QDs, multicolored single-molecule fluorescence imaging of GPCR proteins in Dictyostelium cells is achieved.
Assuntos
Membrana Celular/metabolismo , Halogênios/química , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Imagem Molecular/métodos , Pontos Quânticos , Receptores Acoplados a Proteínas G/metabolismo , Membrana Celular/ultraestrutura , Corantes Fluorescentes/química , Ligantes , Nanoconjugados/química , Nanoconjugados/ultraestrutura , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
There is no confocal microscope optimized for single-molecule imaging in live cells and superresolution fluorescence imaging. By combining the swiftness of the line-scanning method and the high sensitivity of wide-field detection, we have developed a, to our knowledge, novel confocal fluorescence microscope with a good optical-sectioning capability (1.0 µm), fast frame rates (<33 fps), and superior fluorescence detection efficiency. Full compatibility of the microscope with conventional cell-imaging techniques allowed us to do single-molecule imaging with a great ease at arbitrary depths of live cells. With the new microscope, we monitored diffusion motion of fluorescently labeled cAMP receptors of Dictyostelium discoideum at both the basal and apical surfaces and obtained superresolution fluorescence images of microtubules of COS-7 cells at depths in the range 0-85 µm from the surface of a coverglass.
Assuntos
Microscopia Confocal/métodos , Microscopia de Fluorescência/métodos , Imagem Óptica/métodos , Animais , Células COS , Chlorocebus aethiops , Dictyostelium , Transferência Ressonante de Energia de Fluorescência , Microscopia Confocal/instrumentação , Microscopia de Fluorescência/instrumentação , Microtúbulos/química , Imagem Óptica/instrumentação , Receptores de AMP Cíclico/análiseRESUMO
G protein interacting protein 1 (Gip1) binds and sequesters heterotrimeric G proteins in the cytosolic pool, thus regulating G protein-coupled receptor (GPCR) signalling for eukaryotic chemotaxis. Here, we report the underlying structural basis of Gip1 function. The crystal structure reveals that the region of Gip1 that binds to the G protein has a cylinder-like fold with a central hydrophobic cavity composed of six α-helices. Mutagenesis and biochemical analyses indicate that the hydrophobic cavity and the hydrogen bond network at the entrance of the cavity are essential for complex formation with the geranylgeranyl modification on the Gγ subunit. Mutations of the cavity impair G protein sequestration and translocation to the membrane from the cytosol upon receptor stimulation, leading to defects in chemotaxis at higher chemoattractant concentrations. These results demonstrate that the Gip1-dependent regulation of G protein shuttling ensures wide-range gradient sensing in eukaryotic chemotaxis.
Assuntos
Quimiotaxia/fisiologia , Citosol/metabolismo , Proteínas de Ligação ao GTP/química , Proteínas Heterotriméricas de Ligação ao GTP/química , Receptores Acoplados a Proteínas G/química , Fatores Quimiotáticos/química , Cristalografia por Raios X , Dictyostelium , Eucariotos , Proteínas de Ligação ao GTP/genética , Ligação de Hidrogênio , Modelos Moleculares , Mutagênese , Mutação , Ligação Proteica , Conformação Proteica , Domínios e Motivos de Interação entre Proteínas , Proteína Fosfatase 1 , Proteínas de Saccharomyces cerevisiae , Transdução de SinaisRESUMO
Chemotactic cells can exhibit extreme sensitivity to chemical gradients. Theoretical estimations of the signal inputs required for chemotaxis suggest that the response can be achieved under the strong influence of stochastic input noise generated by the receptors during the transmembrane signaling. This arises a fundamental question regarding the mechanisms for directional sensing: how do cells obtain reliable information regarding gradient direction by using stochastically operating receptors and the downstream molecules? To address this question, we have developed single molecule imaging techniques to visualize signaling molecules responsible for chemotaxis in living Dictyostelium cells, allowing us to monitor the stochastic signaling processes directly. Single molecule imaging of a chemoattractant bound to a receptor demonstrates that signal inputs fluctuate with time and space. Downstream signaling molecules, such as PTEN and a PH domain-containing protein that are constituent parts of chemotactic signaling system, can also be followed at single molecule level in living cells, illuminating the stochastic nature of chemotactic signaling processes. In this report, we start with a brief introduction of chemotactic response of the eukaryotic cells, followed by an explanation for single molecule imaging techniques, and finally discuss these applications to chemotactic signaling system of Dictyostelium cells.
Assuntos
Quimiotaxia/fisiologia , Dictyostelium/fisiologia , Animais , AMP Cíclico/fisiologia , Cinética , Microscopia de Fluorescência , PTEN Fosfo-Hidrolase , Transdução de Sinais/fisiologia , Processos Estocásticos , Biologia de SistemasRESUMO
The chemotactic signaling of eukaryotic cells is based on a chain of interactions between signaling molecules diffusing on the cell membrane and those shuttling between the membrane and cytoplasm. In this chapter, we describe methods to quantify lateral diffusion and reaction kinetics on the cell membrane. By the direct visualization and statistic analyses of molecular Brownian movement achieved by single-molecule imaging techniques, multiple states of membrane-bound molecules are successfully revealed with state transition kinetics. Using PTEN, a phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3) 3'-phosphatase, in Dictyostelium discoideum undergoing chemotaxis as a model, each process of the analysis is described in detail. The identified multiple state kinetics provides an essential clue to elucidating the molecular mechanism of chemoattractant-induced dynamic redistribution of the signaling molecule asymmetrically on the cell membrane. Quantitative parameters for molecular reactions and diffusion complement a conventional view of the chemotactic signaling system, where changing a static network of molecules connected by causal relationships into a spatiotemporally dynamic one permits a mathematical description of stochastic migration of the cell along a shallow chemoattractant gradient.
Assuntos
Quimiotaxia , Transdução de Sinais , Imagem Individual de Molécula , Algoritmos , Membrana Celular/metabolismo , Microscopia de Fluorescência , Modelos Biológicos , PTEN Fosfo-Hidrolase/metabolismo , Imagem Individual de Molécula/métodosAssuntos
Quimiotaxia/fisiologia , Diagnóstico por Imagem/métodos , Dictyostelium/fisiologia , Proteínas de Ligação ao GTP/fisiologia , Proteínas de Ligação ao GTP/ultraestrutura , Interpretação de Imagem Assistida por Computador/métodos , Microscopia de Fluorescência/métodos , Biologia Molecular/métodos , Receptores Acoplados a Proteínas G/fisiologia , Receptores Acoplados a Proteínas G/ultraestrutura , Transdução de Sinais/fisiologiaRESUMO
BACKGROUND: The life-cycle of cellular slime molds comprises chronobiologically regulated processes. During the growth phase, the amoeboid cells proliferate at a definite rate. Upon starvation, they synthesize cAMP as both first and second messengers in signalling pathways and form aggregates, migrating slugs, and fruiting bodies, consisting of spores and stalk cells, within 24 h. In Dictyostelium discoideum, because most growth-specific events cease during development, proliferative and heterochronic mutations are not considered to be interrelated and no genetic factor governing the entire life-cycle duration has ever been identified. METHODOLOGY/PRINCIPAL FINDINGS: Using yeast 2-hybrid library screening, we isolated a Dictyostelium discoideum RabGAP, Dd Rbg-3, as a candidate molecule by which the Dictyostelium Gα2 subunit directs its effects. Rab GTPase-activating protein, RabGAP, acts as a negative regulator of Rab small GTPases, which orchestrate the intracellular membrane trafficking involved in cell proliferation. Deletion mutants of Dd rbg-3 exhibited an increased growth rate and a shortened developmental period, while an overexpression mutant demonstrated the opposite effects. We also show that Dd Rbg-3 interacts with 2 Gα subunits in an activity-dependent manner in vitro. Furthermore, both human and Caenorhabditis elegans rbg-3 homologs complemented the Dd rbg-3-deletion phenotype in D. discoideum, indicating that similar pathways may be generally conserved in multicellular organisms. CONCLUSIONS/SIGNIFICANCE: Our findings suggest that Dd Rbg-3 acts as a key element regulating the duration of D. discoideum life-span potentially via trimeric G-protein cascades.
Assuntos
Dictyostelium/genética , Proteínas Ativadoras de GTPase/genética , Estágios do Ciclo de Vida/genética , Proteínas Associadas aos Microtúbulos/genética , Proteínas de Protozoários/genética , Sequência de Aminoácidos , Animais , Caenorhabditis elegans , Proliferação de Células , Dictyostelium/metabolismo , Proteínas Ativadoras de GTPase/metabolismo , Deleção de Genes , Regulação da Expressão Gênica , Teste de Complementação Genética , Humanos , Proteínas Associadas aos Microtúbulos/metabolismo , Dados de Sequência Molecular , Ligação Proteica , Multimerização Proteica , Proteínas de Protozoários/metabolismo , Alinhamento de Sequência , Transdução de Sinais , Técnicas do Sistema de Duplo-HíbridoRESUMO
Environmental changes result in signaling events at cell membranes. To develop the means to understand these events at the molecular level, it is essential to become familiar with the stochastic nature of signaling molecules in living cells. Using total internal reflection fluorescent microscopy (TIRFM), these signaling events can be directly observed at the single-molecule level. This article explains the basis of TIRFM and how it is set up. It then describes how to visualize cell membrane signaling events. It also explains how to prove that detected fluorescence is emitted from single dye molecules and how to analyze the data from TIRFM experiments.
Assuntos
Membrana Celular/química , Técnicas Citológicas/métodos , Dictyostelium/fisiologia , Processamento de Imagem Assistida por Computador/métodos , Microscopia de Fluorescência/métodos , Transdução de Sinais , Animais , Células Cultivadas , Corantes Fluorescentes/metabolismoRESUMO
Environmental changes result in signaling events in cell membranes. To develop the means to understand these events at the molecular level, it is essential to become familiar with the stochastic nature of signaling molecules in living cells. Using total internal reflection fluorescent microscopy (TIRFM), these signaling events can be directly observed at the single-molecule level. This protocol describes the preparation of chemotactically competent Dictyostelium cells. These cells are highly sensitive to chemoattractant stimulation and can be used to visualize single molecules with TIRFM.
Assuntos
Quimiotaxia , Técnicas Citológicas/métodos , Dictyostelium/citologia , Transdução de Sinais , Animais , Técnicas de Cultura de Células/métodos , Células CultivadasRESUMO
Environmental changes result in signaling events at cell membranes. To develop the means to understand these events at the molecular level, it is essential to become familiar with the stochastic nature of signaling molecules in living cells. Using total internal reflection fluorescent microscopy (TIRFM), these signaling events can be directly observed at the single-molecule level. This protocol describes the preparation of an imaging chamber to visualize single molecules in living Dictyostelium cells, which are highly sensitive to chemoattractant stimulation. It also describes treatment of the cells to allow visualization. Chemotactically competent cells are treated with the desired chemical, and the cell suspension is delivered onto coverslips and then overlaid with a thin agarose sheet in preparation for imaging. The cells are typically treated with a fluorescently labeled stimulant or inhibitor. Alternatively, the cells can be stimulated with photoreactive chemicals by using caged compounds. A caged compound is photoactivatable by irradiation with ultraviolet (UV) light, which cleaves a linker conjugating a caging moiety and a chemical. Thus, at a desired moment during recording of the single-molecule images, the concentration of the chemicals can be increased by photolysis of the caged compounds included in the buffer.
Assuntos
Fatores Quimiotáticos/metabolismo , Quimiotaxia , Técnicas Citológicas/métodos , Dictyostelium/fisiologia , Processamento de Imagem Assistida por Computador/métodos , Microscopia de Fluorescência/métodos , Animais , Células Cultivadas , Dictyostelium/efeitos dos fármacos , Corantes Fluorescentes/metabolismo , Transdução de SinaisRESUMO
Environmental changes result in signaling events at cell membranes. To develop the means to understand these events at the molecular level, it is essential to become familiar with the stochastic nature of signaling molecules in living cells. Using total internal reflection fluorescent microscopy (TIRFM), these signaling events can be directly observed at the single-molecule level. This protocol describes the procedure used for objective-type TIRFM to visualize single fluorescent molecules in living Dictyostelium cells, which are highly sensitive to chemoattractant stimulation. This method can also be applied to other cell types.
Assuntos
Membrana Celular/química , Técnicas Citológicas/métodos , Dictyostelium/fisiologia , Processamento de Imagem Assistida por Computador/métodos , Microscopia de Fluorescência/métodos , Transdução de Sinais , Animais , Células Cultivadas , Corantes Fluorescentes/metabolismoRESUMO
In this chapter, we describe methods to monitor signaling events at the single-molecule level on the membrane of living cells by using total internal reflection fluorescence microscopy (TIRFM). The techniques provide a powerful tool for elucidating the stochastic properties of signaling molecules involved in chemotaxis of the cellular slime mold Dictyostelium discoideum. Taking cAMP receptor 1 (cAR1) as an example of a target protein for single-molecule imaging, we describe the experimental setup of TIRFM, a method for labeling cAR1 with a fluorescent dye, and a method for investigating the receptor's lateral mobility. We discuss how the developmental progression of cells modulates both cAR1 behavior and the phenotypic variability in cAR1 mobility for different cell populations.