RESUMO
Pyridine nucleotides such as a nicotinamide adenine dinucleotide (NAD) are known as plant defense activators. We previously reported that nicotinamide mononucleotide (NMN) enhanced disease resistance against fungal pathogen Fusarium graminearum in barley and Arabidopsis. In this study, we reveal that the pretreatment of nicotinamide (NIM), which does not contain nucleotides, effectively suppresses disease development of Fusarium Head Blight (FHB) in wheat plants. Correspondingly, deoxynivalenol (DON) mycotoxin accumulation was also significantly decreased by NIM pretreatment. A metabolome analysis showed that several antioxidant and antifungal compounds such as trigonelline were significantly accumulated in the NIM-pretreated spikes after inoculation of F. graminearum. In addition, some metabolites involved in the DNA hypomethylation were accumulated in the NIM-pretreated spikes. On the other hand, fungal metabolites DON and ergosterol peroxide were significantly reduced by the NIM pretreatment. Since NIM is relative stable and inexpensive compared with NMN and NAD, it may be more useful for the control of symptoms of FHB and DON accumulation in wheat and other crops.
Assuntos
Fusarium/efeitos dos fármacos , Niacinamida/farmacologia , Doenças das Plantas/prevenção & controle , Triticum/microbiologia , Fusarium/metabolismo , Fusarium/patogenicidade , Doenças das Plantas/microbiologia , Tricotecenos/metabolismo , Triticum/efeitos dos fármacosRESUMO
BACKGROUND/AIMS: The aim of this study was to comparatively examine the convenience of use of the QUEST and GerdQ questionnaires as self-administered diagnostic instruments. METHODOLOGY: This was a two-way crossover study conducted from December 2011 to April 2012. The subjects were 70 third-year nursing students of Yokohama Soei University in Yokohama, Japan. They were randomly assigned to fill in either of the study questionnaires first, and then the other on a later. RESULTS: A significant difference was observed in the questionnaire completion time between the QUEST and GerdQ questionnaires (125.5 vs. 44 seconds, P < 0.0001) and also in the number of subjects asking questions while completing the questionnaires (26 vs. 1 subject, respectively: P < 0.0001). To detect GERD based on a QUEST score of ≥4, ROC analysis revealed an area under the curve for the GerdQ score of 0.616. The optimal cutoff value of the GerdQ score was 6, and the sensitivity and specificity calculated using this cutoff value were 0.842 and 0.312, respectively. CONCLUSIONS: This study revealed that Japanese subjects may find it easier to complete the GerdQ than the QUEST questionnaire.
Assuntos
Refluxo Gastroesofágico/diagnóstico , Estudantes de Enfermagem , Inquéritos e Questionários , Adulto , Compreensão , Estudos Cross-Over , Feminino , Humanos , Japão , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Fatores de Tempo , Adulto JovemRESUMO
Primary aldosteronism (PA) is typically managed with mineralocorticoid receptor antagonists (MRAs) barring adrenalectomy. The efficacy of esaxerenone, a nonsteroidal MRA, were explored in patients with PA. Various parameters such as the urinary albumin to creatinine ratio (UACR) and serum levels of N-terminal prohormone of brain natriuretic peptide (NT-proBNP) were evaluated in 25 PA patients before and 3 and 6 months after esaxerenone treatment. Systolic and diastolic blood pressure (BP), and the estimated glomerular filtration rate decreased after treatment, while serum levels of potassium and active renin increased. Significant reductions were observed in UACR 3 and 6 months after treatment. A significant decrease in NT-proBNP was evident at 6 months but not 3 months after treatment. Correlation analysis indicated that the reductions in BP and UACR at 3 months were independent of estimated daily salt intake. Furthermore, the effect of esaxerenone treatment on lowering UACR and NT-proBNP levels was independent of BP reduction. Responders whose systolic BP decreased 6 months after esaxerenone treatment by more than 10 mmHg compared to pretreatment had higher pretreatment NT-proBNP and similar UACR before and after treatment when compared with nonresponders. Esaxerenone improved mental, physical, and social quality of life (QOL) 6 months after treatment compared to healthy controls and increased over time. No patients discontinued treatment due to severe hyperkalemia or renal dysfunction. In conclusion, esaxerenone is a safe and effective MRA for PA treatment, offering significant benefits in terms of hypertension, albuminuria, NT-proBNP levels, and QOL improvement. Esaxerenone effectively lowers BP, UACR, and serum levels of NT-proBNP independent of dietary salt intake in mild PA patients. ARC active renin concentration, DBP diastolic blood pressure, MR mineralocorticoid receptor, MRA mineralocorticoid receptor antagonist, NT-proBNP N-terminal pro-brain natriuretic peptide, PA primary aldosteronism, QOL quality of life, SBP systolic blood pressure, SF-36 Medical Outcomes Study 36-Item Short-Form Health Survey, UACR urinary albumin to creatinine ratio.
Assuntos
Hiperaldosteronismo , Peptídeo Natriurético Encefálico , Humanos , Pressão Sanguínea , Qualidade de Vida , Renina , Creatinina , Albuminas/farmacologia , Hiperaldosteronismo/tratamento farmacológico , Antagonistas de Receptores de Mineralocorticoides/uso terapêutico , Antagonistas de Receptores de Mineralocorticoides/farmacologiaRESUMO
This study investigated the spatiotemporal abundance and diversity of the α-subunit of the dissimilatory sulfite reductase gene (dsrA) in the meromictic Lake Suigetsu for assessing the sulfur-oxidizing bacterial community. The density of dsrA in the chemocline reached up to 3.1 × 10(6) copies ml(-1) in summer by means of quantitative real-time PCR and it was generally higher than deeper layers. Most of the dsrA clones sequenced were related to green sulfur bacteria such as Chlorobium phaeovibrioides, C. limicola, and C. luteolum. Below the chemocline of the lake, we also detected other dsrA clones related to the purple sulfur bacterium Halochromatium salexigens and some branching lineages of diverse sequences that were related to chemotrophic sulfur bacterial species such as Magnetospirillum gryphiswaldense, Candidatus Ruthia magnifica, and Candidatus Thiobios zoothamnicoli. The abundance and community compositions of sulfur-oxidizing bacteria changed depending on the water depth and season. This study indicated that the green sulfur bacteria dominated among sulfur-oxidizing bacterial population in the chemocline of Lake Suigetsu and that certain abiotic environmental variables were important factors that determined sulfur bacterial abundance and community structure.
Assuntos
Chlorobi/isolamento & purificação , Sulfito de Hidrogênio Redutase/genética , Lagos/microbiologia , Chlorobi/genética , Chromatiaceae/genética , Japão , Lagos/química , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase em Tempo RealRESUMO
We measured dietary salt intake in 26 patients with primary aldosteronism treated with mineralocorticoid receptor antagonists and evaluated whether plasma renin levels were affected by dietary salt intake pre-treatment and post 6 months of mineralocorticoid receptor antagonist treatment. The dietary salt intake level was calculated using spot urine sodium and creatinine concentrations, body weight, height, and age. The clinical parameters pre- and post- treatment were compared. The systolic and diastolic blood pressure levels decreased, and the serum potassium and active renin concentration increased significantly. Although the dietary salt intake did not change after treatment, the differences in dietary salt intake and active renin concentration pre- and post- treatment were inversely correlated (r = -0.418, p = 0.03). The 26 patients were divided into two groups with active renin concentration levels ≥5 pg/mL (Group 1) and <5 pg/mL (Group 2) after treatment. The Group parameters did not differ pre- and post- treatment. Group 1 evidenced improvements in systolic and diastolic blood pressures, and the potassium level and active renin concentration over time; Group 2 did not. Group 1 evidenced no significant correlation between the differences in dietary salt intake and active renin concentration levels (r = -0.481, p = 0.11) but Group 2 showed a strong inverse correlation (r = -0.7599, p = 0.01). In conclusion, we found that an active renin concentration level <5 pg/mL post-mineralocorticoid receptor antagonist treatment may indicate that salt sensitivity has not adequately improved, emphasizing the importance of measuring plasma renin levels after such treatment.
Assuntos
Hiperaldosteronismo , Hipertensão , Humanos , Cloreto de Sódio na Dieta , Antagonistas de Receptores de Mineralocorticoides/uso terapêutico , Antagonistas de Receptores de Mineralocorticoides/farmacologia , Renina , Pressão Sanguínea/fisiologia , Potássio , Hiperaldosteronismo/tratamento farmacológico , Hipertensão/tratamento farmacológico , AldosteronaRESUMO
Protein-tyrosine phosphorylation plays a significant role in multiple cellular functions in bacteria. Bacterial tyrosine phosphatases catalyse the dephosphorylation of tyrosyl-phosphorylated proteins. Myxococcus xanthus PhpA shares homology with DNA polymerase and histidinol phosphatase family members. Recombinant His-tagged PhpA requires Mn(2+) or Co(2+) for phosphatase activity, and shows strict specificity for phosphorylated tyrosine residues. The k(m) values of PhpA for p-nitrophenyl phosphate (pNPP) and phosphotyrosine peptide, RRLIEDAEpYAARG, were 803 and 139 µM, respectively. The phosphatase activity of PhpA was inhibited by sodium orthovanadate with a k(i) of 33 µM. phpA gene expression was observed under both vegetative and developmental conditions, but peaked during late fruiting body formation. A phpA mutant exhibited an elevated level of tyrosine phosphorylation of a 79 kDa protein and cytoplasmic tyrosine kinase, BtkA. In M. xanthus, exopolysaccharide (EPS) is essential for cell-cell adhesion and fruiting body formation. phpA mutant cells exhibited enhanced capacity for cell-cell agglutination in agglutination buffer. Under starvation conditions, phpA mutation caused early aggregation and sporulation. The EPS production assay showed that the phpA mutant produced an increased amount of EPS in comparison with the wild-type. These results indicate that PhpA may negatively regulate the production of EPS in M. xanthus.
Assuntos
Myxococcus xanthus/enzimologia , Polissacarídeos/biossíntese , Proteínas Tirosina Fosfatases/metabolismo , Aderência Bacteriana , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Cinética , Mutação , Myxococcus xanthus/genética , Myxococcus xanthus/crescimento & desenvolvimento , Myxococcus xanthus/fisiologia , Proteínas Tirosina Fosfatases/genética , Esporos Bacterianos/fisiologiaRESUMO
Staphylococcal toxic shock syndrome (TSS) is a rare but potentially life-threatening systemic bacterial intoxication. TSS is characterized by fever, hypotension, rash, digestive symptoms such as vomiting and diarrhea, multiorgan system involvement, and desquamation in the initial recovery period. We report a case of a 35-year-old woman who developed TSS caused by methicillin-resistant Staphylococcus aureus following deep inferior epigastric perforator flap breast reconstruction. Local findings are often not obvious in a case of TSS, which can make early diagnosis and appropriate initial treatment difficult. If a patient presents with characteristic symptoms of TSS after autologous breast reconstruction, TSS should be suspected and exploration of surgical wounds should be initiated as soon as possible.
RESUMO
A protein phosphatase, designated Pph3, from Myxococcus xanthus showed the enzymatic characteristics of PP2C-type serine/threonine protein phosphatases, which are metal ion-dependent, okadaic acid-insensitive protein phosphatases. The pph3 mutant under starvation conditions formed immature fruiting bodies and reduced sporulation.
Assuntos
Myxococcus xanthus/enzimologia , Fosfoproteínas Fosfatases/metabolismo , Sequência de Aminoácidos , Cátions/metabolismo , Coenzimas/metabolismo , Inibidores Enzimáticos/metabolismo , Carpóforos/crescimento & desenvolvimento , Metais/metabolismo , Dados de Sequência Molecular , Proteínas Mutantes/metabolismo , Ácido Okadáico/metabolismo , Homologia de Sequência de Aminoácidos , Esporos Fúngicos/crescimento & desenvolvimentoRESUMO
A Myxococcus xanthus cytoplasmic bacterial tyrosine kinase, BtkA, showed phosphorylation activity in the presence of Exo. Phosphorylated BtkA was expressed late after starvation induction and early after glycerol induction. The btkA mutant was unable to complete maturation to heat- and sonication-resistant spores under both starvation- and glycerol-induced developmental conditions.
Assuntos
Proteínas de Bactérias/metabolismo , Myxococcus xanthus/enzimologia , Proteínas Tirosina Quinases/metabolismo , Esporos Bacterianos/crescimento & desenvolvimento , Sequência de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Glicerol/metabolismo , Temperatura Alta , Dados de Sequência Molecular , Myxococcus xanthus/química , Myxococcus xanthus/genética , Myxococcus xanthus/crescimento & desenvolvimento , Proteínas Tirosina Quinases/química , Proteínas Tirosina Quinases/genética , Alinhamento de SequênciaRESUMO
Sulfur-oxidizing bacterial diversity at the surface of cattle manure was characterized throughout the composting process using a sulfur oxidation gene (soxB) clone library approach. In the mesophilic phase, clones related to the genera Hydrogenophaga and Hydrogenophilus were characteristically detected. In the thermophilic phase, clones related to the genera Hydrogenophaga and Thiohalobacter were predominant. In the cooling phase, the predominant soxB sequences were related to the genus Pseudaminobacter and a new sulfur-oxidizing bacterium belonging to the class Alphaproteobacteria. The present study showed changes in the community composition of sulfur-oxidizing bacteria at the surface of compost throughout the composting process.
Assuntos
Bactérias/metabolismo , Compostagem , Esterco/microbiologia , Enxofre/metabolismo , Animais , Bactérias/classificação , Bactérias/genética , Bactérias/crescimento & desenvolvimento , Biodiversidade , Bovinos , Genes Bacterianos/genética , Esterco/análise , Microbiota , Oxirredução , Filogenia , TemperaturaRESUMO
Reactive oxygen species (ROS) are generated in the cell through multiple mechanisms. Intracellular ROS are rapidly detoxified by various enzymatic and non-enzymatic mechanisms; however, disruption of the oxidant-antioxidant balance causes oxidative stress and elicits cell damage. The oxidative stress induced by chemotherapy is known to cause side effects in patients with cancer. However, few studies have examined whether anticancer drugs induce oxidative stress in cancer cells. Furthermore, the precise mechanism by which anticancer drugs induce the generation of ROS remains unclear. In the present study, to investigate whether anticancer drugs induce oxidative stress, DLD-1 human colorectal cancer cells were treated with 20 different anticancer drugs and then stained with CellROX® ROS detection reagent. Furthermore, an oxygen radical absorbance capacity assay in the presence of copper was performed to estimate the oxidative activities of the anticancer drugs in the absence of cells. The data of the present study using assay methods in the presence and absence of cells suggest that nimustine, actinomycin D, doxorubicin, mitomycin C, mitoxantrone, carmofur, gemcitabine, mercaptopurine, camptothecin, paclitaxel, vinblastine, and vinorelbine are able to induce oxidative stress.
RESUMO
We attempted to develop a pretreatment method for methane fermentation of lignocellulosic biomass using cattle rumen fluid, treated as slaughterhouse waste. When rapeseed (Brassica napus L.) was added to the methane fermentation after being solubilized with rumen fluid, 1.5 times more methane was produced compared with untreated rapeseed. Analysis of the bacterial flora during rumen fluid treatment using the MiSeq next-generation sequencer showed that the predominant phylum shifted from Bacteroidetes, composed of amylolytic Prevotella spp., to Firmicutes, composed of cellulolytic and xylanolytic Ruminococcus spp., in only 6 h. In total, 7 cellulolytic, 25 cello-oligosaccharolytic, and 11 xylanolytic bacteria were detected after investigating the most abundant sequences of detected taxa. The relative abundance of two Ruminococcus species (Ruminococcus albus and R. flavefaciens), known as cellulolytic, cello-oligosaccharolytic, and xylanolytic bacteria, increased with increasing cellulose and hemicellulose degradation rates, and, finally, comprised 48% of all operational taxonomic units. The chronological observation of enzyme activities showed that cellulolytic and xylanolytic activities increased 6 h later, and that oligosaccharolytic activity increased 24 h later. This study detected six bacteria that participate in the degradation of aromatics derived from lignin, which have rarely been reported in rumen fluid. The constitution of the detected bacteria suggests that the aromatics were converted into acetate via benzoate. The list of microbes that cover all lignocellulose-degrading candidates will provide fundamental knowledge for future studies focusing on rumen microbes.
Assuntos
Biomassa , Líquidos Corporais/enzimologia , Líquidos Corporais/microbiologia , Lignina/metabolismo , Metano/biossíntese , Rúmen/enzimologia , Rúmen/microbiologia , Matadouros , Animais , Bacteroidetes/isolamento & purificação , Bacteroidetes/metabolismo , Brassica napus/química , Bovinos , Celulose/metabolismo , Fermentação , Polissacarídeos/metabolismo , Ruminococcus/isolamento & purificação , Ruminococcus/metabolismo , Gerenciamento de ResíduosRESUMO
Under paddy field conditions, biological sulfur oxidation occurs in the oxidized surface soil layer and rhizosphere, in which oxygen leaks from the aerenchyma system of rice plants. In the present study, we examined community shifts in sulfur-oxidizing bacteria associated with the oxidized surface soil layer and rice roots under different sulfur fertilization conditions based on the 16S ribosomal RNA (rRNA) gene in order to explore the existence of oligotrophic sulfur-oxidizing bacteria in the paddy rice ecosystem. Rice plants were grown in pots with no fertilization (control) or CaCO3 or CaSO4 fertilization. A principal-coordinates analysis (PCoA) showed that CaSO4 fertilization markedly affected bacterial communities associated with rice roots and soil, whereas no significant differences were observed in plant growth among the fertilizer treatments examined. In rice roots, the relative abundance of Acidobacteria, Alphaproteobacteria, Gammaproteobacteria, and TM7 was significantly higher in CaSO4-fertilized pots than in control pots. Alphaproteobacteria, Bradyrhizobiaceae, and Methylocystaceae members were significantly more abundant in CaSO4-fertilized roots than in control roots. On the other hand, the abundance of Actinobacteria and Proteobacteria was lower in CaSO4-fertilized soil than in control soil. These results indicate that the bacteria associated with rice roots and soil responded to the sulfur amendment, suggesting that more diverse bacteria are involved in sulfur oxidation in the rice paddy ecosystem than previously considered.
Assuntos
Bactérias/classificação , Bactérias/efeitos dos fármacos , Biota/efeitos dos fármacos , Oryza/microbiologia , Raízes de Plantas/microbiologia , Microbiologia do Solo , Enxofre/metabolismo , Bactérias/genética , Sulfato de Cálcio/metabolismo , DNA Ribossômico/química , DNA Ribossômico/genética , Fertilizantes , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
Myxococcus xanthus MXAN_0575, ArsA, exhibited sequence homology to low-molecular-weight protein tyrosine phosphatases (LMWPTPs) and arsenate reductases. ArsA exhibited weak phosphatase activity toward p-nitrophenyl phosphate, and high arsenate reductase activity, suggesting that ArsA may play a role in arsenate reductase, but not LMWPTP.
Assuntos
Arseniato Redutases/metabolismo , Proteínas de Bactérias/metabolismo , Myxococcus xanthus/enzimologia , Arseniato Redutases/química , Proteínas de Bactérias/química , Peso Molecular , Proteínas Tirosina Fosfatases/química , Homologia de Sequência de AminoácidosRESUMO
Myxococcus xanthus BtkB is composed of an N-terminal periplasmic domain and a C-terminal cytoplasmic tyrosine kinase domain. The C-terminal cytoplasmic domain of BtkB was autophosphorylated in the presence of [γ-(32) P]ATP and MgCl(2), and the autophosphorylated BtkB was detected with antiphosphotyrosine antibody, suggesting that BtkB is a bacterial tyrosine (BY) kinase. BY kinases have been demonstrated in the production of extracellular polysaccharide (EPS), antibiotic resistance, stress response, and DNA metabolism. Myxococcus xanthus btkB gene was expressed mainly in the growth phase and early stages of fruiting body development. When cultured in nutrient medium at high temperature (37 °C), btkB mutant showed reduced maximum cell density as compared to the wild type. Under starvation conditions, btkB mutant cells formed fruiting bodies and spores about 24 h later than the wild-type strain. The btkB mutant overproduced yellow pigment during development. Also, btkB mutant showed a decrease in EPS production when compared with the wild-type strain. These results suggested that BtkB may play multiple roles in M. xanthus cells.
Assuntos
Proteínas de Bactérias/metabolismo , Myxococcus xanthus/enzimologia , Proteínas Tirosina Quinases/metabolismo , Sequência de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Regulação Bacteriana da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Dados de Sequência Molecular , Myxococcus xanthus/química , Myxococcus xanthus/genética , Myxococcus xanthus/crescimento & desenvolvimento , Fosforilação , Polissacarídeos Bacterianos/biossíntese , Proteínas Tirosina Quinases/química , Proteínas Tirosina Quinases/genética , Alinhamento de SequênciaRESUMO
The Myxococcus xanthus protein phosphatase Pph3 belongs to the Mg(2+)- or Mn(2+)-dependent protein phosphatase (PPM) family. Bacterial PPMs contain three divalent metal ions and a flap subdomain. Putative metal- or phosphate-ion binding site-specific mutations drastically reduced enzymatic activity. Pph3 contains a cyclic nucleotide monophosphate (cNMP)-binding domain in the C-terminal region, and it requires 2-mercaptoethanol for phosphatase activity; however, the C-terminal deletion mutant showed high activity in the absence of 2-mercaptoethanol. The phosphatase activity of the wild-type enzyme was higher in the presence of cAMP than in the absence of cAMP, whereas a triple mutant of the cNMP-binding domain showed slightly lower activities than those of wild-type, without addition of cAMP. In addition, mutational disruption of a disulphide bond in the wild-type enzyme increased the phosphatase activity in the absence of 2-mercaptoethanol, but not in the C-terminal deletion mutant. These results suggested that the presence of the C-terminal region may lead to the formation of the disulphide bond in the catalytic domain, and that disulphide bond cleavage of Pph3 by 2-mercaptoethanol may occur more easily with cAMP bound than with no cAMP bound.
Assuntos
Aminoácidos/genética , Proteínas de Bactérias/metabolismo , Sequência Conservada/genética , Manganês/metabolismo , Myxococcus xanthus/enzimologia , Fosfoproteínas Fosfatases/metabolismo , Sequência de Aminoácidos , Proteínas de Bactérias/química , AMP Cíclico/farmacologia , Dissulfetos/metabolismo , Cinética , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , Myxococcus xanthus/efeitos dos fármacos , Fosfoproteínas Fosfatases/química , Ligação Proteica/efeitos dos fármacos , Relação Estrutura-Atividade , Especificidade por Substrato/efeitos dos fármacosRESUMO
Fish farm sediments receive a large amount of organic matter from uneaten food and fecal material. This nutrient enrichment, or organic pollution, causes the accumulation of sulphide in the sediment from the action of sulphate-reducing bacteria (SRB). We investigated the effect of organic enrichment around coastal fish farms comparing the SRB community structure in these sediments. Sediment samples with different levels of organic pollution classified based upon the contents of acid-volatile sulphide and chemical oxygen demand were collected at three stations on the coast of western Japan. The SRB community composition was assessed using PCR amplification, cloning, sequencing and phylogenetic analysis of the dissimilatory sulphite reductase b-subunit gene (dsrB) fragments using directly extracted sediment DNA. Sequencing of the cloned PCR products of dsrB showed the existence of different SRB groups in the sediments. The majority of dsrB sequences were associated with the families Desulfobacteraceae and Desulfobulbaceae. Clones related to the phylum Firmicutes were also detected from all sediment samples. Statistical comparison of sequences revealed that community compositions of SRB from polluted sediments significantly differed from those of moderately polluted sediments and unpolluted sediments (LIBSHUFF, p<0.05), showing a different distribution of SRB in the fish farm sediments. There is evidence showing that the organic enrichment of sediments influences the composition of SRB communities in sediments at marine fish farms.
Assuntos
Bactérias/classificação , Bactérias/metabolismo , Biota , Sedimentos Geológicos/microbiologia , Sulfatos/metabolismo , Aquicultura , Bactérias/genética , Bactérias/isolamento & purificação , Clonagem Molecular , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , Sedimentos Geológicos/química , Sulfito de Hidrogênio Redutase/genética , Japão , Dados de Sequência Molecular , Compostos Orgânicos/análise , Oxirredução , Filogenia , Reação em Cadeia da Polimerase , Análise de Sequência de DNARESUMO
A new set of primers for the detection of phototrophic sulfur bacteria in natural environments is described. The primers target the α-subunit of the reverse dissimilatory sulfite reductase gene (dsrA). PCR-amplification resulted in products of the expected size from all the phototrophic strains tested, including purple sulfur and green sulfur bacteria. Seventy-nine clones obtained from environmental DNA using the primers were sequenced and all found to be closely related to the dsrA of purple sulfur bacteria and green sulfur bacteria. This newly developed PCR assay targeting dsrA is rapid and simple for the detection of phototrophic sulfur bacteria in situ and superior to the use of culture-dependent techniques.