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1.
J Environ Public Health ; 2022: 2350522, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35256886

RESUMO

The COVID-19 pandemic led to the suspension of all university courses which was followed directly by the implementation of online learning in Thailand. However, online learning was not suitable for all of Thailand. Rangsit University is a famous private university in Thailand and has been affected by this crisis, so it attempted to eliminate online learning by offering vaccination and antigen rapid screening tests to the students and staff who had to attend the university from July to September 2021. 93.71% of the students and staff from Rangsit University who attended the university from July to September 2021 were vaccinated. Only 1.18% of the students and staff were infected. The vaccines used were CoronaVac and AstraZeneca at 66.02% and 33.98%, respectively. The percentage of individuals that were infected after vaccination did not differ between the two vaccines. The percentage of people infected was 0.31% for CoronaVac and 0.29% for AstraZeneca. Other important factors that influenced the infection rate were the initial symptoms and the environment. Individuals who had initial symptoms and had visited areas with high-risk factors had a high possibility of becoming infected. This research is intended to be useful for risk management during the COVID-19 crisis.


Assuntos
COVID-19 , COVID-19/diagnóstico , COVID-19/epidemiologia , Humanos , Pandemias , Prevalência , SARS-CoV-2 , Estudantes , Tailândia/epidemiologia , Universidades
2.
Asian Pac J Cancer Prev ; 8(2): 294-6, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17696749

RESUMO

Glutathione S-transferases (GSTs) are enzymes that involved in bio- transformation by conjugation of electrophillic compounds to glutathione. Polymorphisms within genes that encode GSTs may affect the function of the enzymes. Polymorphisms of GSTP1 at codon 105 residue forms GSTP1 active site for binding of hydrophobic electrophiles, and the Ile-Val substitution affect substrate specific catalytic activity of this enzyme and may associate with susceptibility to malignant human disease, especially acute lymphoblastic leukemia (ALL), which is the most common leukemia in children younger than 15 years old. Genetic polymorphisms within the GSTP1 gene of childhood ALL patients were studied. In addition, the association of genetic polymorphism of GSTP1 and genetic susceptibility of acute lymphoblastic leukemia (ALL) was also determined using Chi-square and Odds ratio. PCR-RFLP was used to study genetic polymorphism of GSTP1 in 100 ALL patients and 100 healthy individuals.The results show that there is no statistically significant association between each genotypes and genetic susceptibility of acute lymphoblastic leukemia (ALL) (OR=0.92, P -value=0.886). Moreover, there is no statistically significant association between each genotypes and demographic data of acute lymphoblastic leukemia (ALL). However, there are 2 cases of ALL with BM relapse show the polymorphic genotypes of GSTP1. It may suggest that GSTP1*V105 may be involved in relapse of ALL.


Assuntos
Predisposição Genética para Doença , Glutationa S-Transferase pi/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Adolescente , Substituição de Aminoácidos , Criança , Pré-Escolar , Primers do DNA , DNA de Neoplasias/genética , DNA de Neoplasias/isolamento & purificação , Feminino , Frequência do Gene , Genótipo , Humanos , Lactente , Isoleucina , Masculino , Polimorfismo de Fragmento de Restrição , Leucemia-Linfoma Linfoblástico de Células Precursoras/enzimologia , Resultado do Tratamento , Valina
3.
J Microbiol ; 51(4): 522-6, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23990305

RESUMO

Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei.


Assuntos
Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Burkholderia pseudomallei/genética , Burkholderia pseudomallei/metabolismo , Mutação , Actinas/metabolismo , Animais , Proteína 1 de Membrana Associada ao Lisossomo/metabolismo , Macrófagos/metabolismo , Macrófagos/microbiologia , Melioidose/metabolismo , Melioidose/microbiologia , Camundongos , Ligação Proteica
4.
FEMS Microbiol Lett ; 323(1): 75-82, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22092682

RESUMO

Burkholderia pseudomallei, the causative agent of melioidosis, exploits the Bsa type III secretion system (T3SS) to deliver effector proteins into host cells. These effectors manipulate host cell functions; thus, contributing to the ability of the bacteria to evade the immune response and cause disease. Only two Bsa-secreted effectors have been conclusively identified to date. Here, we report the identification of the third B. pseudomallei type III secreted effector protein, designated BopC. BopC is encoded by the bpss1516 gene abutting bpss1517, which encodes its putative chaperone. The genes are located in the close proximity to the bsa T3SS gene cluster of B. pseudomallei K96243 (Fig. 1). BopC was secreted into culture supernatant by the wild-type B. pseudomallei strain, but its secretion was abolished in the bsaZ T3SS mutant. Using pull down and co-purification assays, we confirmed that BopC interacts with its putative chaperone, BPSS1517, in vitro. Furthermore, the first 20 N-terminal amino acids of BopC were found to be sufficient to mediate the T3SS-dependent translocation of a reporter protein from a heterologous enteropathogenic Escherichia coli host into mammalian cells. Finally, bopC mutant was found to be less invasive than the wild-type strain in the epithelial cells.


Assuntos
Proteínas de Bactérias/metabolismo , Burkholderia pseudomallei/metabolismo , Burkholderia pseudomallei/patogenicidade , Fatores de Virulência/metabolismo , Proteínas de Bactérias/genética , Burkholderia pseudomallei/genética , Linhagem Celular , Células Epiteliais/microbiologia , Escherichia coli/genética , Deleção de Genes , Genes Reporter , Humanos , Chaperonas Moleculares/metabolismo , Ligação Proteica , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Fatores de Virulência/genética
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