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Concentrated polymer brushes (CPBs), which are significantly denser and thicker than conventional semidilute polymer brushes, have received increasing attention in the field of tribology because of their superlow friction properties. However, despite numerous studies aimed at enhancing CPBs for mechanical applications, the relationship between the specific layered structure and lubrication mechanisms of CPBs is still not completely understood. In this study, to reveal the relationship, simultaneous time-resolved measurements of the interfacial gap, static mechanical response, and dynamic mechanical response of the CPB at the contact interface were conducted using optical interference and precise force measuring methods. Two types of tests (i.e., the "indentation" and "sliding" tests) were alternately performed on a glass substrate coated with the CPB against a steel ball immersed in an ionic liquid. The indentation tests measuring the time-resolved interfacial gap and changes in static and dynamic mechanical responses quantitatively confirmed the presence of dilute, middle, and concentrated layers in the CPB. In the sliding tests, the wear of the CPB was detected by observing a decrease in the interfacial gap at the contact interface. Moreover, the thickness of the dilute layer remained constant with sliding, whereas the thicknesses of the other layers decreased, indicating that the dilute layer was continuously formed due to sliding. Therefore, CPB wear occurs randomly at the friction interface alongside the formation of a dilute layer with low density and stiffness on the surface.
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BACKGROUND: Mobile health (mHealth) apps are considered to be potentially powerful tools for improving lifestyles and preventing cardiovascular disease (CVD), although only few have undergone large, well-designed epidemiological research. "kencom" is a novel mHealth app with integrated functions for healthy lifestyles such as monitoring daily health/step data, providing tailored health information, or facilitating physical activity through group-based game events. The app is linked to large-scale Japanese insurance claims databases and annual health check-up databases, thus comprising a large longitudinal cohort. OBJECTIVE: We aimed to assess the effects of kencom on physical activity levels and CVD risk factors such as obesity, hypertension, dyslipidemia, and diabetes mellitus in a large population in Japan. METHODS: Daily step count, annual health check-up data, and insurance claim data of the kencom users were integrated within the kencom system. Step analysis was conducted by comparing the 1-year average daily step count before and after kencom registration. In the CVD risk analysis, changes in CVD biomarkers following kencom registration were evaluated among the users grouped into the quintile according to their change in step count. RESULTS: A total of 12,602 kencom users were included for the step analysis and 5473 for the CVD risk analysis. The participants were generally healthy and their mean age was 44.1 (SD 10.2) years. The daily step count significantly increased following kencom registration by a mean of 510 steps/day (P<.001). In particular, participation in "Arukatsu" events held twice a year within the app was associated with a remarkable increase in step counts. In the CVD risk analysis, the users of the highest quintile in daily step change had, compared with those of the lowest quartile, a significant reduction in weight (-0.92 kg, P<.001), low-density lipoprotein cholesterol (-2.78 mg/dL, P=.004), hemoglobin A1c (HbA1c; -0.04%, P=.004), and increase in high-density lipoprotein cholesterol (+1.91 mg/dL, P<.001) after adjustment of confounders. CONCLUSIONS: The framework of kencom successfully integrated the Japanese health data from multiple data sources to generate a large, longitudinal data set. The use of the kencom app was significantly associated with enhanced physical activity, which might lead to weight loss and improvement in lipid profile.
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Doenças Cardiovasculares , Aplicativos Móveis , Telemedicina , Adulto , Biomarcadores , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/prevenção & controle , Exercício Físico , Estilo de Vida Saudável , Fatores de Risco de Doenças Cardíacas , Humanos , Fatores de RiscoRESUMO
Both neuromyelitis optica spectrum disorder (NMOSD) and Wernicke's encephalopathy (WE) involve brain lesions. However, their treatments are quite different. In this report, we describe the case of a 29-year-old woman with NMOSD, who presented with clinical and imaging findings similar to those of WE. She was admitted to our hospital with a headache, vomiting, and loss of appetite for two weeks and diplopia for nine days. Magnetic resonance imaging revealed lesions in the area postrema, periaqueductal gray matter, thalamus, and right frontal lobe. Vitamin B1 supplementation was ineffective. The patient was diagnosed with NMOSD because serum aquaporin-4 antibody was detected after admission. Her symptoms improved with immunotherapy. The possibility of NMOSD should be considered in patients with suspected WE.
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Adsorbed molecular films, referred to as boundary films in tribology, are widely used in various industrial products as a keyway for surface functionalisation, such as lubricity, wettability, and adhesion. Because boundary films are thin nanometre-scale molecular layers and can easily be removed, their formation process cannot be elucidated in detail. In this study, to analyse the growth dynamics of boundary films, the film thickness and molecular orientation of the boundary film of a fatty acid used as an additive in rolling contact as mechanical stimuli were measured in situ. The measurements were performed on simple test lubricants, which were composed of n-hexadecane and stearic acid, at rolling tribological condition between steel and glass (or sapphire) surfaces by ultrathin film interferometry combined with sum-frequency generation spectroscopy according to a unique protocol. The results quantitatively demonstrate shear-induced boundary film formation. The insight gained from these results is anticipated to enable the formulation of high-performance lubricant additives to further reduce friction loss and high-performance glues that can be freely designed for removability.
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Friction continues to account for the bulk of energy losses in mechanical systems, with an estimated 23% of the world's total energy consumption used to overcome friction. Concentrated polymer brushes (CPBs) have recently attracted significant scientific and industrial attention, given their ability to achieve superlubricity (i.e., coefficients of friction below 0.01); however, understanding the mechanistic interactions underlying their wear performance has been largely overlooked. Herein, we employ a custom-built optical test apparatus to investigate the inter-dependencies between CPBs and laser-produced surface texture (LST), assessing for the first time the friction, film thickness, and wear behavior in situ and simultaneously. Recent developments in picosecond laser etching allowed us to graft CPBs atop the finest laser-etched matrix of micron-sized dimples reported in literature to date. At low sliding speeds, combined CPB-LST reduces the coefficient of friction to 0.0006, while increasing the CPB durability by up to 34% through a lateral support mechanism offered by the textured micro-features. Furthermore, the imaging results shed light on CPB failure mechanisms. Both these mechanisms of lateral support and failure propagation impact the wear resistance of CPBs and are important in the development of CPBs for future applications (e.g., in low-speed bearings functioning under controlled abrasive wear conditions).
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Since the discovery of synchronous pulsations in cardiomyocytes (CMs), electrical communication between CMs has been emphasized; however, recent studies suggest the possibility of mechanical communication. Here, we demonstrate that spherical self-beating CM aggregates, termed cardiac spheroids (CSs), produce enhanced mechanical energy under mechanical compression and work cooperatively via mechanical communication. For single CSs between parallel plates, compression increased both beating frequency and beating energy. Contact mechanics revealed a scaling law on the beating energy, indicating that the most intensively stressed cells in the compressed CSs predominantly contributed to the performance of mechanical work against mechanical compression. For pairs of CSs between parallel plates, compression immediately caused synchronous beating with mechanical coupling. Compression tended to strengthen and stabilize the synchronous beating, although some irregularity and temporary arrest were observed. These results suggest that mechanical compression is an indispensable control parameter when evaluating the activities of CMs and their aggregates.
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Modelos Cardiovasculares , Miócitos Cardíacos/fisiologia , Fenômenos Biomecânicos , Agregação Celular/fisiologia , Comunicação Celular/fisiologia , Células Cultivadas , Força Compressiva/fisiologia , Humanos , Contração Miocárdica/fisiologia , Miócitos Cardíacos/citologia , Esferoides Celulares/citologia , Esferoides Celulares/fisiologia , Estresse MecânicoRESUMO
In the textbook formulation of dry friction laws, static and dynamic friction (stick and slip) are qualitatively different and sharply separated phenomena. However, accurate measurements of stick-slip motion generally show that static friction is not truly static but characterized by a slow creep that, upon increasing tangential load, smoothly accelerates into bulk sliding. Microscopic, contact-mechanical, and phenomenological models have been previously developed to account for this behavior. In the present work, we show that it may instead be a systemic property of the measurement apparatus. Using a mechanical model that exhibits the characteristics of typical setups of measuring friction forces-which usually have very high transverse stiffness-and assuming a small but nonzero misalignment angle in the contact plane, we observe some fairly counterintuitive behavior: Under increasing longitudinal loading, the system almost immediately starts sliding perpendicularly to the pulling direction. Then the friction force vector begins to rotate in the plane, gradually approaching the pulling direction. When the angle between the two becomes small, bulk sliding sets in quickly. Although the system is sliding the entire time, macroscopic stick-slip behavior is reproduced very well, as is the accelerated creep during the "stick" phase. The misalignment angle is identified as a key parameter governing the stick-to-slip transition. Numerical results and theoretical considerations also reveal the presence of high-frequency transverse oscillations during the "static" phase, which are also transmitted into the longitudinal direction by nonlinear processes. Stability analysis is carried out and suggests dynamic probing methods for the approaching moment of bulk slip and the possibility of suppressing stick-slip instabilities by changing the misalignment angle and other system parameters.
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Tributyltin (TBT) compounds have been detected in fish and shellfish. One of the targets of TBT compounds is the central nervous system. Alterations in the levels of neurotransmitters and their metabolites, and ratios of the levels of neurotransmitters to those of their metabolites have been used as indexes of neurotoxicity. We evaluated the neurotoxicity of TBT compounds in mice following subacute oral exposure by determining the levels of neurotransmitters and their metabolites in discrete brain regions. Male BALB/c mice were exposed to 0, 1, 5, 25, or 125 ppm TBT chloride in their feed for one month. Following the treatment period, their brains were removed and dissected into the cerebrum, cerebellum, medulla oblongata, midbrain, corpus striatum and hypothalamus. The levels of norepinephrine, dopamine (DA), dihydoxyphenylacetic acid, homovanillic acid (HVA), serotonin, and 5-hydroxyindolacetic acid were determined in different brain regions by high-performance liquid chromatography (HPLC). The mean body weight of mice treated with 125 ppm TBT was significantly lower than that of the control from day 5 to day 16 during the treatment period. The HVA/DA ratio in the midbrain of the 125 ppm-treated group was significantly higher than those of other treatment groups, and tended to be higher than that of the control. TBT may affect DA metabolism in the brain, especially in the midbrain.
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Encéfalo/efeitos dos fármacos , Dopamina/metabolismo , Poluentes Ambientais/toxicidade , Compostos de Trialquitina/toxicidade , Ácido 3,4-Di-Hidroxifenilacético/metabolismo , Administração Oral , Animais , Encéfalo/metabolismo , Química Encefálica , Ingestão de Alimentos/efeitos dos fármacos , Ácido Homovanílico/metabolismo , Ácido Hidroxi-Indolacético/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Neurotransmissores/metabolismo , Norepinefrina/metabolismo , Serotonina/metabolismo , Fatores de Tempo , Redução de Peso/efeitos dos fármacosRESUMO
The sliding of a solid object on a solid substrate requires a shear force that is larger than the maximum static friction force. It is commonly believed that the maximum static friction force is proportional to the loading force and does not depend on the apparent contact area. The ratio of the maximum static friction force to the loading force is called the static friction coefficient µM, which is considered to be a constant. Here, we conduct experiments demonstrating that the static friction force of a slider on a substrate follows a novel friction law under certain conditions. The magnitude of µM decreases as the loading force increases or as the apparent contact area decreases. This behavior is caused by the slip of local precursors before the onset of bulk sliding and is consistent with recent theory. The results of this study will develop novel methods for static friction control.
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Diffuse alveolar hemorrhage (DAH) syndrome is potentially fatal. We encountered a nearly fatal case of DAH possibly due to intravenous itraconazole (ITCZ). A 53-year-old man with chronic pulmonary aspergillosis underwent pneumonectomy of the left lung 15 days prior to the onset of DAH, which was confirmed by bronchoalveloar lavage. The battery of diagnostic evaluations performed revealed no other positive etiological factor, leading to the diagnosis of DAH possibly induced by intravenous ITCZ with a positive drug lymphocyte stimulation test. The patient did not respond to pulse methylprednisolone therapy, but responded dramatically to direct hemoperfusion using a polymyxin B-immobilized fiber column (PMX) therapy.
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Antifúngicos/efeitos adversos , Hemorragia/induzido quimicamente , Itraconazol/efeitos adversos , Pneumopatias/induzido quimicamente , Antifúngicos/administração & dosagem , Antifúngicos/uso terapêutico , Doença Crônica , Hemoperfusão , Hemorragia/terapia , Humanos , Injeções Intravenosas , Itraconazol/administração & dosagem , Itraconazol/uso terapêutico , Pneumopatias/terapia , Masculino , Pessoa de Meia-Idade , Polimixina B , Aspergilose Pulmonar/tratamento farmacológicoRESUMO
OBJECTIVE: Tributyltin (TBT) compounds have been widely used as antifouling agents for shipbottom paint. The immune system is a target of TBT intoxication. We evaluated the effects of TBT chloride in macrophages, which have critical roles in the immune system, using a murine macrophage lineage cell line, J774.1,in vitro. METHODS: We examined tumor necrosis factor α (TNFα), interleukin-1ß (IL-1ß) andc-jun mRNA expression in J774.1 cells. The effects of TBT on the apoptosis of J774.1 cells were examined by determining the percentage of TUNEL-positive cells and caspase-3 activity. RESULTS: The mean values of the viabilities of J774.1 cells exposed to TBT decreased dose-dependently. The relative mRNA expression of TNFα increased dose-dependently, however, that of IL-1ß was not significantly different among the groups. The mean percentage of TUNEL-positive cells increased dose-dependently. Increases in the caspase-3 activities of J774.1 cells were observed in the groups exposed to higher concentrations of TBT. The mean value of relative mRNA expression of c-Jun transcription factor increased dose-dependently. CONCLUSIONS: The increases in the percentage of TUNEL-positive cells and in caspase-3 activity suggested that exposure to TBT induces apoptosis of J774.1 cells. The increases in the mRNA expression of TNFα andc-jun by TBT may be related to apoptosis in macrophages.