RESUMO
BACKGROUND: In resource-limited settings in which child malnutrition is prevalent, humans live in close proximity to household livestock. However, the relation between household livestock and child nutrition represents a considerable knowledge gap. OBJECTIVE: We assessed whether household livestock ownership or livestock disease episodes were associated with growth in young children in western Kenya. METHODS: We incorporated monthly anthropometric measurements for children <5 y of age into an ongoing linked human and animal surveillance cohort in rural western Kenya. Using linear mixed models adjusted for age, sex, and household wealth, we tested whether baseline household livestock ownership was related to baseline child height for age or prospective growth rate. We also evaluated whether livestock disease episodes were associated with child growth rate over 11 mo of follow-up. RESULTS: We collected data on 925 children over the course of follow-up. Greater household livestock ownership at baseline was not related to baseline child height-for-age z score (adjusted ß: 0.01 SD; 95% CI: -0.02, 0.04 SD) or child growth rate (adjusted ß: 0.02 cm/y; 95% CI: -0.03, 0.07 cm/y). Livestock disease episodes were not significantly associated with child growth across the entire cohort (adjusted ß: -0.007 cm/mo; 95% CI: -0.02, 0.006 cm/mo). However, children in households with livestock digestive disease between June and November gained less height than did children in households that did not report livestock disease (ß: -0.063 cm/mo; 95% CI: -0.112, -0.016 cm/mo). Children <2 y of age in households with livestock digestive disease gained less weight than did those who did not report disease (ß: -0.033 kg/mo; 95% CI: -0.063, -0.003 kg/mo). CONCLUSION: In this cohort of young children in western Kenya, we did not find an association between ownership of livestock and child growth status. However, disease episodes in household livestock may be related to a lower child growth rate in some groups.
Assuntos
Doenças dos Animais , Transtornos da Nutrição Infantil/complicações , Características da Família , Transtornos do Crescimento/etiologia , Crescimento , Gado , Propriedade , Animais , Estatura , Criança , Pré-Escolar , Doenças do Sistema Digestório/veterinária , Feminino , Humanos , Lactente , Quênia , Masculino , Estado Nutricional , Estudos Prospectivos , População Rural , Aumento de PesoRESUMO
BACKGROUND: The epidemiology and burden of influenza remain poorly defined in sub-Saharan Africa. Since 2005, the Kenya Medical Research Institute and Centers for Disease Control and Prevention-Kenya have conducted population-based infectious disease surveillance in Kibera, an urban informal settlement in Nairobi, and in Lwak, a rural community in western Kenya. METHODS: Nasopharyngeal and oropharyngeal swab specimens were obtained from patients who attended the study clinic and had acute lower respiratory tract (LRT) illness. Specimens were tested for influenza virus by real-time reverse-transcription polymerase chain reaction. We adjusted the incidence of influenza-associated acute LRT illness to account for patients with acute LRT illness who attended the clinic but were not sampled. RESULTS: From March 2007 through February 2010, 4140 cases of acute LRT illness were evaluated in Kibera, and specimens were collected from 1197 (27%); 319 (27%) were positive for influenza virus. In Lwak, there were 6733 cases of acute LRT illness, and specimens were collected from 1641 (24%); 359 (22%) were positive for influenza virus. The crude and adjusted rates of medically attended influenza-associated acute LRT illness were 6.9 and 13.6 cases per 1000 person-years, respectively, in Kibera, and 5.6 and 23.0 cases per 1000 person-years, respectively, in Lwak. In both sites, rates of influenza-associated acute LRT illness were highest among children <2 years old and lowest among adults ≥50 years old. CONCLUSION: In Kenya, the incidence of influenza-associated acute LRT illness was high in both rural and urban settings, particularly among the most vulnerable age groups.
Assuntos
Influenza Humana/epidemiologia , Influenza Humana/virologia , Pneumonia Viral/epidemiologia , Pneumonia Viral/virologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Quênia/epidemiologia , Masculino , Pessoa de Meia-Idade , Nasofaringe/virologia , Orofaringe/virologia , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , População Rural , Estações do Ano , Adulto JovemRESUMO
SETTING: In sub-Saharan Africa, high rates of tuberculosis (TB) and human immunodeficiency virus (HIV) infection pose a serious threat for occupationally acquired TB among health care workers. OBJECTIVE: To identify factors associated with TB disease among staff of an 1800-bed hospital in Kenya. DESIGN: We calculated TB incidence among staff and conducted a case-control study where cases (n = 65) were staff diagnosed with TB and controls (n = 316) were randomly selected staff without recent TB. RESULTS: The annual incidence of TB from 2001 to 2005 ranged from 645 to 1115 per 100000 population. Factors associated with TB disease were additional daily hours spent in rooms with patients (adjusted odds ratio [aOR] 1.3, 95%CI 1.2-1.5), working in areas where TB patients received care (aOR 2.1, 95%CI 1.1-4.2), HIV infection (aOR 29.1, 95%CI 5.1-167) and living in a slum (aOR 4.7, 95%CI 1.8-12.5) or hospital-provided low-income housing (aOR 2.6, 95%CI 1.2-5.6). CONCLUSION: Hospital exposures were associated with TB disease among staff at this hospital regardless of their job designation, even after controlling for living conditions, suggesting transmission from patients. Health care facilities should improve infection control practices, provide quality occupational health services and encourage staff testing for HIV infection to address the TB burden in hospital staff.
Assuntos
Pessoal de Saúde , Transmissão de Doença Infecciosa do Paciente para o Profissional , Tuberculose/transmissão , Adulto , Feminino , Infecções por HIV/complicações , Hospitais Públicos , Habitação , Humanos , Quênia , Masculino , Fatores de Risco , Tuberculose/epidemiologia , Adulto JovemRESUMO
We previously showed that Theiler's murine encephalomyelitis virus (TMEV)-infected major histocompatibility complex (MHC) class II-deficient mice develop both demyelination and neurologic deficits, whereas MHC class I-deficient mice develop demyelination but no neurologic deficits. The absence of neurologic deficits in the class I-deficient mice was associated with preserved sodium channel densities in demyelinated lesions, a relative preservation of axons, and extensive spontaneous remyelination. In this study, we investigated whether TMEV-infected class II-deficient mice, which have an identical genetic background (C57BL/6 x 129) as the class I-deficient mice, have preserved axons and spontaneous myelin repair following chronic TMEV-infection. Both class I- and class II-deficient mice showed similar extents of demyelination of the spinal cord white matter 4 months after TMEV infection. However, the class I-deficient mice demonstrated remyelination by oligodendrocytes, whereas class II-deficient mice showed minimal if any myelin repair. Demyelinated lesions, characterized by inflammatory infiltrates in both mutants, revealed disruption of axons in class II- but not class I-deficient mice. Further characterization revealed that even though class II-deficient mice lacked TMEV-specific IgG, they had virus-specific IgM, which, however, did not neutralize TMEV in vitro. In addition, class II-deficient mice developed TMEV-specific cytotoxic T-lymphocytes in the CNS during the acute (7 days) disease, but these cytotoxic lymphocytes were not present in the chronic stage of disease, despite a high titer of infectious virus throughout the disease. We envision that the presence of demyelination, high virus titer, absence of remyelination, and axonal disruption in chronically infected class II-deficient mice contributes to the development of paralytic disease.
Assuntos
Infecções por Cardiovirus/fisiopatologia , Antígenos de Histocompatibilidade Classe II/fisiologia , Bainha de Mielina/fisiologia , Regeneração Nervosa , Medula Espinal/fisiologia , Theilovirus , Doença Aguda , Animais , Antígenos Virais/imunologia , Axônios/ultraestrutura , Infecções por Cardiovirus/imunologia , Doença Crônica , Imunoglobulina M/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Medula Espinal/imunologia , Linfócitos T Citotóxicos/imunologiaRESUMO
Mice with targeted deletion of L-selectin gene (L-sel(-/-)) were used to investigate the role of adhesion molecule in immunologic responses following virus infection in the central nervous system (CNS). L-Sel(-/-) mice from a resistant H-2(b) genetic background and parental wild-type H-2(b) (C57BL/6) mice were infected with Theiler's murine encephalomyelitis virus (TMEV) intracerebrally and the kinetics of virus replication and infiltration of immune cells in the CNS determined. The levels of infectious TMEV, as measured by plaque assay at 3, 7, 14, and 28 days after infection were between 4 and 6 log(10) PFU of virus per gram of CNS tissues at days 3 and 7 post-infection, and then decreased to undetectable levels by day 14 after infection in both strains of mice. The L-sel(-/-) mice had decreased numbers of CD8(+) T lymphocytes (17.72%+/-2.4) infiltrating into the CNS at 7 days post-infection when compared to wild-type mice (31.02%+/-7.5). In addition, the L-sel(-/-) mice had significantly lower levels of TMEV-specific serum IgG resulting in lower virus neutralizing activity of the serum when compared to wild-type mice. However, the L-sel(-/-) mice had 2.5-fold increase in B lymphocytes in the CNS (8.29%+/-1.1) when compared to wild-type mice (3.2%+/-0.4). Taken together, these data indicate that L-selectin plays a role in recruitment of B and CD8(+) T lymphocytes into the CNS following virus infection, which, however, did not affect the ability of the mice to clear TMEV infection.
Assuntos
Linfócitos T CD8-Positivos/virologia , Selectina L/genética , Esclerose Múltipla/imunologia , Poliomielite/imunologia , Theilovirus , Animais , Anticorpos Antivirais/sangue , Linfócitos B/imunologia , Linfócitos B/virologia , Linfócitos T CD8-Positivos/imunologia , Sistema Nervoso Central/imunologia , Sistema Nervoso Central/virologia , Modelos Animais de Doenças , Citometria de Fluxo , Imunoglobulina G/sangue , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Esclerose Múltipla/virologia , Poliomielite/genética , Baço/citologia , Replicação ViralRESUMO
A contemporary view of autoimmunity suggests that self-reactivity is a normal phenomenon, in contrast to the classical association between autoimmunity and immunopathology. We have previously demonstrated that monoclonal antibody SCH94.03, a natural autoantibody with polyreactivity towards several purified protein and hapten antigens, promotes central nervous system remyelination when passively transferred to SJL/J mice chronically infected with Theiler's murine encephalomyelitis virus, an established experimental model of multiple sclerosis. In this study we characterized the autoreactivity of SCH94.03 with endogenous mouse tissue using immunoperoxide and multiple-color immunofluorescence staining techniques on frozen tissue sections. Within the nervous system, SCH94.03 labeled fibrous astrocytes, ependymal cells, ganglion satellite cells, and a sub-population of microglia, oligodendrocytes, and peripheral nervous system neurons. Outside the nervous system, SCH94.03 labeled gastrointestinal tract smooth muscle and luminal epithelium, erythrocytes, and interdigitating dendritic cells in peripheral lymphoid organs. These data indicate that SCH94.03 is a multi-organ reactive autoantibody and support the hypothesis that autoantibodies can have a beneficial rather than a pathogenic function in central nervous system demyelinating diseases.
Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/farmacologia , Autoanticorpos/imunologia , Esclerose Múltipla/imunologia , Bainha de Mielina/imunologia , Bainha de Mielina/fisiologia , Animais , Autoimunidade/imunologia , Autoimunidade/fisiologia , Sistema Nervoso Central/imunologia , Epitélio/imunologia , Imunofluorescência , Imuno-Histoquímica , Tecido Linfoide/imunologia , Camundongos , Camundongos Endogâmicos , Músculo Liso/imunologia , Sistema Nervoso Periférico/imunologia , Ratos , Ratos Sprague-DawleyRESUMO
Recombinant proteins to the LP, VP1, VP2, VP3, VP4, 2A, 2B, 2C, 3A, and 3D genes of Theiler's murine encephalomyelitis virus (TMEV) were generated and antibodies were produced against them for use in analysis of the TMEV epitopes responsible for eliciting the antibody responses observed during acute and chronic disease. Antibodies against recombinant VP1, VP2, and VP3 recognized the corresponding proteins from purified TMEV particles. In immunohistochemical analysis, antibodies against recombinant capsid (VP1, VP2, and VP3), and non-capsid (2A, 2C, 3A) proteins were reactive with PO-2D cells (astrocytes) infected with TMEV in vitro and with brain tissues of acutely infected mice. Antibodies against VP4, 2B, and 3D antigens were not reactive with corresponding viral proteins in infected astrocytes cells or brain tissues, but they reacted with TMEV precursor proteins produced during the early viral replication phase. Sera from SJL/J mice infected with TMEV acutely (14 days) and chronically (45 days) reacted with VP1, VP2, VP4, 2A, and 2C proteins. In an in vitro assay for neutralization, only anti-VP1 antibodies neutralized TMEV infection. These findings suggest that both capsid and non-capsid proteins of TMEV play a role in the immunopathology of the TMEV disease in the central nervous system.
Assuntos
Anticorpos Antivirais/sangue , Encéfalo/virologia , Capsídeo/imunologia , Infecções por Cardiovirus/virologia , Proteínas Virais/imunologia , Animais , Anticorpos Antivirais/imunologia , Astrócitos/virologia , Western Blotting , Encéfalo/imunologia , Capsídeo/análise , Capsídeo/genética , Infecções por Cardiovirus/imunologia , Linhagem Celular , Reações Cruzadas , Camundongos , Camundongos Endogâmicos C57BL , Coelhos , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Theilovirus/genética , Theilovirus/imunologia , Theilovirus/isolamento & purificação , Proteínas Virais/análise , Proteínas Virais/genéticaRESUMO
Avian pneumovirus (APV) causes a respiratory disease in turkeys. The virus has been associated with morbidity and mortality due to secondary infections. Our objective was to determine if APV caused immunosuppression in the T-cell or B-cell compartments and to study the pathogenesis of the disease in APV maternal antibody-lacking 2-wk-old commercial turkeys. APV was administered by the eyedrop/intranasal route. Observations were made for gross lesions, viral genome, and T-cell mitogenesis and cytokine secretion at 3, 5, 7, 14, and 21 days postinoculation (DPI). During the acute phase of the disease that lasted for about 1 wk, the turkeys exposed to APV showed clinical signs characterized by nasal discharge and sinus swelling. Virus genome was detected by in situ hybridization in cells of turbinates and trachea at 3 and 5 DPI. At 3 and 5 DPI, spleen cells of the birds infected with APV markedly decreased proliferative response to concanavalin A (Con A). Con A and lipopolysaccharide stimulation of spleen cells from virus-exposed turkeys resulted in accumulation of nitric oxide-inducing factors (NOIF) in the culture fluid. NOIF were not detected in culture fluids of Con A-stimulated spleen cells of virus-free turkeys. APV did not compromise the antibody-producing ability of turkeys against several extraneous antigens such as Brucella abortus and tetanus toxoid.
Assuntos
Infecções por Pneumovirus/veterinária , Pneumovirus/imunologia , Pneumovirus/patogenicidade , Doenças das Aves Domésticas/virologia , Perus , Administração Intranasal , Animais , Anticorpos Antivirais/biossíntese , Citocinas/metabolismo , Ensaio de Imunoadsorção Enzimática/veterinária , Hibridização In Situ/veterinária , Pneumovirus/isolamento & purificação , Infecções por Pneumovirus/imunologia , Infecções por Pneumovirus/patologia , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/patologia , Baço/citologia , Baço/virologia , Linfócitos T/imunologia , Fatores de TempoRESUMO
Choanal cleft swab samples from 770 wild Canada geese (Branta canadensis) and 358 blue-winged teal (Anas discors), captured for relocation or banding, were examined for the presence of avian pneumovirus (APV) RNA by reverse transcription (RT)-polymerase chain reaction (PCR) and for virus isolation. The swab samples were pooled into groups of 5 or 10. Sixty eight of 102 (66.7%) pooled goose samples were RT-PCR positive for APV RNA. Thirteen of 52 (25.0%) pooled blue-winged teal samples were RT-PCR positive for APV RNA. APV RNA-positive samples were inoculated onto chick embryo fibroblasts (CEF) and QT-35 cells. Infectious APV was isolated from five Canada goose pooled samples in CEF and from one Canada goose pool in QT-35 cells but not from blue-winged teal.
Assuntos
Patos/virologia , Gansos/virologia , Pneumovirus/isolamento & purificação , Animais , Animais Selvagens/virologia , Técnica Indireta de Fluorescência para Anticorpo , Pneumovirus/classificação , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Eleven market turkey flocks developed a respiratory disease characterized by coughing, swollen sinuses and nasal discharge. These symptoms first appeared between 3 and 16 days of age. Avian pneumovirus (APV) RNA was detected by reverse transcriptase (RT)-polymerase chain reaction (PCR) in six of six flocks tested. APV was detected by immunohistochemistry in turbinates of three of three affected flocks tested. Virus isolation attempts were negative. Ten of 11 flocks became seropositive on the APV enzyme-linked immunosorbent assay. Five weeks prior to hatch of these affected market turkeys, several breeder flocks in one geographic area had developed clinical signs and experienced decline in egg production typical of APV infection. In two breeder flocks, acute and convalescent sera indicated APV infection during the period of declining egg production. Attempts to detect APV RNA by RT-PCR from choanal cleft swabs of newly hatched poults were successful. Attempts to isolate the virus from these PCR-positive samples were negative.
Assuntos
Surtos de Doenças/veterinária , Infecções por Pneumovirus/veterinária , Pneumovirus/isolamento & purificação , Doenças das Aves Domésticas/epidemiologia , Perus , Fatores Etários , Animais , Ensaio de Imunoadsorção Enzimática/veterinária , Imuno-Histoquímica/veterinária , Pneumovirus/genética , Infecções por Pneumovirus/diagnóstico , Infecções por Pneumovirus/epidemiologia , Infecções por Pneumovirus/virologia , Doenças das Aves Domésticas/diagnóstico , Doenças das Aves Domésticas/virologia , RNA Viral/genética , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Conchas Nasais/virologiaRESUMO
To detect avian pneumovirus (APV) in central North America, nasal turbinates or choanal deft tissues from domestic turkeys and wild birds were examined for the presence of APV RNA by reverse transcriptase-polymerase chain reaction (RT-PCR), whereas serum samples from domestic turkeys were analyzed for APV antibodies by enzyme-linked immunosorbent assay (ELISA). In 2002, the seroprevalence of disease in domestic turkeys in Minnesota remained high (42.3% of the flocks). In addition, there is evidence the disease has spread to turkey flocks in North Dakota (8.2%), South Dakota (7%), Iowa (10%), and Wisconsin (8.6%) as detected by RT-PCR and/or ELISA. House sparrows and ring-billed gulls sampled in Minnesota and snow geese from Saskatchewan, Canada, were found to harbor APV RNA. Sequence analysis of wild bird APV strains showed high amino acid sequence identity among wild bird isolates (<97%) and between wild bird and turkey viral isolates (93.2%-99.3%). This study demonstrated that APV infections were present in domestic turkey flocks and wild birds outside the state of Minnesota; however, the role of wild birds in spreading APV to domestic turkeys remains unclear.
Assuntos
Doenças das Aves/epidemiologia , Metapneumovirus , Infecções por Paramyxoviridae/veterinária , Sequência de Aminoácidos , Animais , Animais Selvagens/virologia , Anticorpos Antivirais/sangue , Doenças das Aves/virologia , Aves/virologia , Metapneumovirus/genética , Metapneumovirus/imunologia , Dados de Sequência Molecular , América do Norte/epidemiologia , Infecções por Paramyxoviridae/epidemiologia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/virologia , RNA Viral/isolamento & purificação , Alinhamento de Sequência , Perus/virologia , Proteínas da Matriz Viral/químicaRESUMO
OBJECTIVE: To determine the susceptibility of ducks to avian pneumovirus (APV) of turkey origin. ANIMALS: 30 Pekin ducks that were 2 weeks old. PROCEDURE: Ducks were assigned to 3 groups (10 ducks/group). Ducks of groups 1 and 2 were inoculated (day 0) with 200 microl of cell-culture fluid containing APV of turkey origin (10(5.5) median tissue-culture infective dose/ml) by the oculonasal (group 1) or oral (group 2) route. Ducks of group 3 served as noninoculated control birds. Two ducks from each group were euthanatized 3, 6, 9, 15, and 21 days after inoculation. Blood samples, tissue samples from the lungs, trachea, nasal turbinates, duodenum, diverticulum vitellinum (Meckel's diverticulum), and cecum, and swab specimens from the choana, cloaca, and trachea were obtained from all birds during necropsy and examined for APV by use of reverse transcriptase-polymerase chain reaction (RT-PCR), virus isolation, and histologic examination. Blood samples also were examined for APV antibodies, using an ELISA. RESULTS: Tissue samples obtained up to 21 days after inoculation had positive results when tested by use of RT-PCR. Virus was isolated from nasal turbinates of birds inoculated via the oculonasal route. Serum samples obtained 15 and 21 days after inoculation had positive results when tested for APV-specific antibody. Clinical signs of disease were not observed in ducks inoculated with APV of turkey origin. CONCLUSIONS AND CLINICAL RELEVANCE: Ducks inoculated with APV of turkey origin may not develop clinical signs of disease, but they are suspected to play a role as nonclinical carriers of APV.
Assuntos
Patos/virologia , Infecções por Pneumovirus/veterinária , Pneumovirus/crescimento & desenvolvimento , Doenças das Aves Domésticas/virologia , Perus/virologia , Animais , Anticorpos Antivirais/biossíntese , Anticorpos Antivirais/sangue , Chlorocebus aethiops , DNA Viral/química , Patos/imunologia , Ensaio de Imunoadsorção Enzimática , Histocitoquímica/veterinária , Minnesota , Pneumovirus/imunologia , Infecções por Pneumovirus/sangue , Infecções por Pneumovirus/imunologia , Infecções por Pneumovirus/virologia , Doenças das Aves Domésticas/imunologia , RNA Viral/química , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Conchas Nasais/virologia , Células VeroRESUMO
In a survey of intestinal canine zoonotic helminths in Nairobi, 156 dogs were autopsied. Of these, 10% were infected with Echinococcus granulosus, 88% with Ancylostoma caninum, 45% with Dipylidium caninum and 3% with Toxocara canis. All the infected dogs with E. granulosus originated from around abottoirs in Dagoretti. The risk of infection in man with hydatid disease is considered to be high around Dagoretti and control measures are recommended.
Assuntos
Doenças do Cão/epidemiologia , Helmintíase Animal , Enteropatias Parasitárias/veterinária , Saúde Pública , Zoonoses , Matadouros , Animais , Doenças do Cão/parasitologia , Doenças do Cão/prevenção & controle , Doenças do Cão/transmissão , Cães , Inquéritos Epidemiológicos , Helmintíase/epidemiologia , Helmintíase/parasitologia , Helmintíase/prevenção & controle , Helmintíase/transmissão , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Enteropatias Parasitárias/prevenção & controle , Quênia/epidemiologia , Vigilância da População , Prevalência , Fatores de Risco , Saúde da População UrbanaRESUMO
In the past year, animal studies on central nervous system demyelination have focused on therapeutic strategies and the functional characterization of T-cells, cytokines, and adhesion molecules. Significant progress has been realized in the treatment of animals with experimentally induced demyelinating disease using glial cell transplants, autoantibodies, cytokines, growth factors, and by inhibiting adhesion molecules and cytokines. In this review, we summarize the important contributions made in those respective areas.
Assuntos
Doenças Desmielinizantes/fisiopatologia , Modelos Animais de Doenças , Animais , Autoantígenos/metabolismo , Citocinas/metabolismo , Modelos NeurológicosRESUMO
In our previous studies of the early pathogenesis of the Marek's disease virus (MDV)-associated model of atherosclerosis, the brachiocephalic arteries and ascending aortas of MDV-inoculated chickens failed to develop lipid accretion and intimal/medial proliferation consistent with atherosclerotic lesions, as described in the original reports of this model. The role of cholesterol supplementation in the formation of MDV-associated atherosclerotic lesions was reexamined. At 3 days of age, 40 chicks were inoculated with the CU-2 strain of MDV. Another 40 chicks were sham inoculated. At 15 weeks postinfection, half of the sham- and MDV-inoculated birds received 2% cholesterol supplementation in the diet for the rest of the experimental period. At 30 weeks postinfection, the aortas and brachiocephalic arteries were evaluated. Several observations were different from the original description of the model. None of the chickens among the four experimental groups developed atherosclerotic lesions, regardless of MDV inoculation or cholesterol supplementation. However, intimal thickening and marked oil red O-positive foam cell accumulation were observed in all 11 MDV-inoculated, cholesterol-supplemented chickens. None of the nine MDV-inoculated, unsupplemented chickens manifested arterial lipid accumulation, despite the presence of an intimal cellular infiltrate. Also in contrast to the original model description, both cholesterol-supplemented and unsupplemented MDV-inoculated chickens manifested significant increases in serum cholesterol in comparison with the respective sham-inoculated groups.
Assuntos
Galinhas/virologia , Colesterol/sangue , Herpesvirus Galináceo 2/isolamento & purificação , Metabolismo dos Lipídeos , Doença de Marek/metabolismo , Doenças das Aves Domésticas/metabolismo , Túnica Íntima/metabolismo , Animais , Aorta/metabolismo , Aorta/patologia , Arteriosclerose/etiologia , Arteriosclerose/patologia , Arteriosclerose/veterinária , Tronco Braquiocefálico/metabolismo , Tronco Braquiocefálico/patologia , Colesterol na Dieta/farmacologia , Modelos Animais de Doenças , Alimentos Fortificados , Herpesvirus Galináceo 2/fisiologia , Lipídeos/análise , Doença de Marek/sangue , Doença de Marek/patologia , Doenças das Aves Domésticas/etiologia , Doenças das Aves Domésticas/patologia , Distribuição Aleatória , Túnica Íntima/químicaRESUMO
Nasal turbinates or swabs were collected from wild ducks, geese, owls, sparrows, swallows, and starlings and from sentinel ducks placed next to turkey farms experiencing avian pneumovirus (APV) infections and were analyzed for APV genome and infectious particles. APV RNA was detected in samples examined from geese, sparrows, and starlings. APV RNA and antibodies were also detected in two different groups of sentinel ducks. Infectious APV was recovered from sentinel duck samples. The APV M gene isolated from the wild birds had over 96% predicted amino acid identity with APV/Minnesota 2A, which was isolated earlier from domestic turkeys showing respiratory illness, suggesting that wild birds may be involved in spreading APV infection.
Assuntos
Doenças das Aves/virologia , Infecções por Pneumovirus/veterinária , Pneumovirus/isolamento & purificação , Doenças das Aves Domésticas/virologia , RNA Viral/genética , Perus/virologia , Sequência de Aminoácidos , Animais , Animais Selvagens/virologia , Aves/virologia , Dados de Sequência Molecular , Pneumovirus/genética , Infecções por Pneumovirus/virologia , RNA Viral/análise , Vigilância de Evento Sentinela/veterinária , Homologia de Sequência de Aminoácidos , Estados Unidos , Proteínas da Matriz Viral/química , Proteínas da Matriz Viral/genéticaRESUMO
Cells of the central nervous system (CNS) normally do not express detectable levels of major histocompatibility complex (MHC) Class I antigens. However, MHC Class I expression can be induced after virus infection. We tested the hypothesis that virus-induced Class I expression is mediated by lymphocytes or cytokines using lymphocyte- and cytokine-deficient mice. We used Theiler's murine encephalomyelitis virus (TMEV), which induces CNS demyelination that maps genetically to the D region of MHC Class I and is associated with high levels of Class I products. TMEV infection of severe combined immunodeficiency (SCID) and recombination activation gene-1-deficient mice, which lack B and T lymphocytes, resulted in equivalent H-2D and H-2K expression in brain and spinal cord, according to analysis of the area and intensity of immunoperoxidase staining. Class I antigens were demonstrated as early as 6 hours after infection, and they were more widely distributed than viral RNA, indicating that expression was induced indirectly via a soluble factor. To determine whether cytokines induced the expression, we infected mice lacking receptors for interferon-alpha/beta (IFN-alpha/beta R (-/-)), interferon-gamma (IFN-gamma R(-/-)), and tumor necrosis factor-alpha (TNFRp55(-/-)). TMEV-infected IFN-gamma R(-/-) and TN-FRp55(-/-) mice expressed Class I antigens in the CNS, whereas IFN-alpha/beta R(-/-) mice did not, establishing that IFN-alpha/beta mediated the expression. In contrast to the equivalent expression in SCID mice, we observed greater area and higher intensity of H-2D versus H-2K antigens in infected SCID mice reconstituted with normal spleen cells. Collectively, the data indicate that after TMEV infection, early generalized MHC Class I expression is mediated by IFN-alpha/beta independently of lymphocytes, but the differential regulation of H-2D over H-2K may be controlled by B and/or T lymphocytes.
Assuntos
Sistema Nervoso Central/imunologia , Antígenos H-2/biossíntese , Proteínas de Homeodomínio , Interferon-alfa/metabolismo , Interferon beta/metabolismo , Poliomielite/imunologia , Theilovirus , Animais , Encéfalo/metabolismo , Plexo Corióideo/imunologia , Proteínas de Ligação a DNA/imunologia , Proteínas de Ligação a DNA/fisiologia , Citometria de Fluxo , Processamento de Imagem Assistida por Computador , Técnicas Imunoenzimáticas , Hibridização In Situ , Camundongos , Camundongos Endogâmicos C57BL , Camundongos SCID , Neuroglia/metabolismo , Neurônios/metabolismo , RNA Viral/metabolismo , Imunodeficiência Combinada Severa/imunologia , Medula Espinal/metabolismo , Theilovirus/patogenicidade , Regulação para CimaRESUMO
The mechanisms by which type I interferons (IFN) reduce the rate and severity of exacerbations in multiple sclerosis are unknown. We utilized a model of multiple sclerosis to determine the extent of demyelination and remyelination in Theiler's murine encephalomyelitis virus (TMEV)-infected SJL/J mice treated with mouse IFN-alpha/beta for a short (5 weeks) or a long (16 weeks) period. All mice were chronically infected with TMEV to simulate the clinical situation in multiple sclerosis. Short-term IFN-alpha/beta treatment increased the percent of remyelinated spinal cord white matter by threefold when compared with phosphate-buffered saline (PBS) treatment (P < 0.02), but it did not affect the extent of demyelination. In contrast, long-term IFN-alpha/beta treatment increased the extent of demyelination by twofold (P < 0.03). Long-term treatment increased the absolute area of remyelination, but the percent remyelination as a function of area of demyelination was not changed because of increased demyelination. An immunomodulatory mechanism may have contributed to the effect of IFN-alpha/beta on white matter pathology because treated mice had higher anti-TMEV IgGs in serum and demonstrated decreased numbers of B and T lymphocytes infiltrating the central nervous system (CNS). There was no correlation between the level of anti- IFN-alpha/beta antibodies and the extent of demyelination or remyelination. These results indicate that the length of type I IFN treatment may have paradoxical effects on demyelination and remyelination.
Assuntos
Antivirais/uso terapêutico , Interferon-alfa/uso terapêutico , Interferon beta/uso terapêutico , Esclerose Múltipla/tratamento farmacológico , Esclerose Múltipla/fisiopatologia , Bainha de Mielina/efeitos dos fármacos , Bainha de Mielina/fisiologia , Animais , Anorexia/induzido quimicamente , Antivirais/efeitos adversos , Linfócitos B/patologia , Infecções por Cardiovirus/patologia , Infecções por Cardiovirus/virologia , Epitopos , Hipersensibilidade Tardia/imunologia , Imunoglobulina G/imunologia , Imunoglobulina G/metabolismo , Interferon-alfa/efeitos adversos , Interferon beta/efeitos adversos , Camundongos , Camundongos Endogâmicos , Esclerose Múltipla/patologia , Oligodendroglia/fisiologia , Medula Espinal/efeitos dos fármacos , Medula Espinal/metabolismo , Medula Espinal/patologia , Medula Espinal/virologia , Linfócitos T/patologia , Theilovirus/imunologia , Theilovirus/isolamento & purificação , Fatores de TempoRESUMO
Animal models with selective genetic immunodeficiencies are useful tools to identify pathogenic mechanisms of disease. Resistant (C57BL/6F 129/J) (H-2b) mice are rendered susceptible to Theiler's murine encephalomyelitis virus-induced demyelination by genetic disruption of the beta 2 microglobulin gene [beta 2 m(-l-)]. The absence of beta 2 m prevents the expression of major histocompatibility complex class I molecules and normal levels of functional CD8+ T cells. We tested whether genetic depletion of beta 2 m would permit CNS remyelination after chronic demyelination induced by the Daniel's strain of Theiler's virus. In contrast to the minimal spontaneous remyelination observed in SJL/J mice after infection with the Daniel's strain of Theiler's virus, chronically infected beta 2 m(-I-) mice showed extensive and progressive spontaneous CNS remyelination at 6, 12, and 18 months after infection. Spontaneous remyelination by both oligodendrocytes and Schwann cells occurred despite the presence of persistent virus antigen and RNA, but was associated with diminished virus-specific humoral and delayed-type hypersensitivity responses. These experiments support the hypothesis that the immune response inhibits myelin regeneration after virus-induced CNS demyelination.
Assuntos
Doenças Desmielinizantes/fisiopatologia , Bainha de Mielina/fisiologia , Poliomielite/fisiopatologia , Medula Espinal/fisiopatologia , Theilovirus , Microglobulina beta-2/deficiência , Animais , Anticorpos Antivirais/imunologia , Antígenos Virais/análise , Doença Crônica , Doenças Desmielinizantes/patologia , Feminino , Hipersensibilidade Tardia/etiologia , Hipersensibilidade Tardia/fisiopatologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Poliomielite/imunologia , Poliomielite/patologia , RNA Viral/metabolismo , Medula Espinal/metabolismo , Medula Espinal/patologia , Theilovirus/imunologiaRESUMO
We addressed the contributions of persistent virus infection and immune cells to the pathogenesis of Theiler's virus-induced demyelination, a model for human multiple sclerosis. We developed a model involving the transfer of spleen cells into immunodeficient C.B-17-scid (SCID) mice, which normally die of overwhelming virus encephalitis without demyelination when infected with Theiler's virus. Adoptive transfer of nonimmune spleen cells from BALB/c mice into SCID mice resulted in the survival of all mice. However, these mice developed extensive demyelination and virus Ag/RNA persistence in the spinal cord white matter. The most demyelination was observed when mice received an intermediate number of spleen cells (1.8-7.5 x 10(6)), whereas too few cells (0.5 x 10(6)) did not ameliorate the SCID phenotype, and too many cells (30 x 10(6)) resulted in almost complete viral clearance with minimal demyelination. Adoptive transfer of spleen cells depleted of either CD4+ or CD8+ T cells produced vacuolar demyelination associated with virus persistence. In contrast, reconstitution with both CD4+ and CD8+ T cells produced less severe demyelination and partial clearance of virus. These experiments support the hypothesis that demyelination is the result of a balance between persistent virus infection and immune injury mediated by either CD4+ or CD8+ T cells.