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1.
An Acad Bras Cienc ; 93(suppl 3): e20210134, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34787172

RESUMO

Campomanesia xanthocarpa (Mart.) O. Berg is a South American fruit tree species with important ecological and medicinal properties, which remnants are currently found mainly in isolated forest fragments. In this study, SSR markers from three different genomic origins (gene-linked, nuclear neutral, and organellar) were used to evaluate the patterns of genetic diversity, fine-scale spatial genetic structure and historical gene flow in fragmented forest formations of C. xanthocarpa from the Atlantic Forest in southern Brazil. Our results show that the forest fragments present moderate to high levels of genetic diversity in comparison to species presenting similar life traits, although a trend opposite to expected was observed concerning gene-linked and neutral SSR markers. The fine-scale spatial genetic structure revealed different patterns in short and large distance classes, with a distinct influence of gene-linked and neutral markers in driving the genetic structure in each distance class. The presence of an isolation-by-adaptation pattern implies the need for maintenance of the current remnants to assure the conservation of the private alleles. Finally, as the genetic diversity is found predominantly within forest fragments, programs of seed collection and/or genetic rescue should prioritize a larger number of individuals within each fragment, to increase the sampled diversity.


Assuntos
Fluxo Gênico , Variação Genética , Alelos , Fluxo Gênico/genética , Variação Genética/genética , Genômica , Humanos , Árvores/genética
2.
Planta ; 247(4): 1011-1030, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29340796

RESUMO

MAIN CONCLUSION: The plastome of macaw palm was sequenced allowing analyses of evolution and molecular markers. Additionally, we demonstrated that more than half of plastid protein-coding genes in Arecaceae underwent positive selection. Macaw palm is a native species from tropical and subtropical Americas. It shows high production of oil per hectare reaching up to 70% of oil content in fruits and an interesting plasticity to grow in different ecosystems. Its domestication and breeding are still in the beginning, which makes the development of molecular markers essential to assess natural populations and germplasm collections. Therefore, we sequenced and characterized in detail the plastome of macaw palm. A total of 221 SSR loci were identified in the plastome of macaw palm. Additionally, eight polymorphism hotspots were characterized at level of subfamily and tribe. Moreover, several events of gain and loss of RNA editing sites were found within the subfamily Arecoideae. Aiming to uncover evolutionary events in Arecaceae, we also analyzed extensively the evolution of plastid genes. The analyses show that highly divergent genes seem to evolve in a species-specific manner, suggesting that gene degeneration events may be occurring within Arecaceae at the level of genus or species. Unexpectedly, we found that more than half of plastid protein-coding genes are under positive selection, including genes for photosynthesis, gene expression machinery and other essential plastid functions. Furthermore, we performed a phylogenomic analysis using whole plastomes of 40 taxa, representing all subfamilies of Arecaceae, which placed the macaw palm within the tribe Cocoseae. Finally, the data showed here are important for genetic studies in macaw palm and provide new insights into the evolution of plastid genes and environmental adaptation in Arecaceae.


Assuntos
Arecaceae/genética , Genes de Plantas/genética , Plastídeos/genética , Evolução Molecular , Filogenia
3.
PLoS Genet ; 7(5): e1002064, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21589895

RESUMO

The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme--GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species.


Assuntos
Genoma de Planta , Herbaspirillum/genética , Cromossomos de Plantas , Herbaspirillum/metabolismo , Interações Hospedeiro-Patógeno , Fixação de Nitrogênio , Pressão Osmótica , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo
4.
3 Biotech ; 10(6): 273, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32523867

RESUMO

The aim of this study was to develop simple sequence repeat (SSR) markers for genetic studies on G. chacoensis, as well as to evaluate their transferability to other bamboo species. Genomic DNA was isolated from G. chacoensis and its partial sequencing was used to find SSR loci. The obtained sequencing data were de novo assembled using the software CLC Genomics Workbench® 8.0v. The SSR loci primers were identified and designed with the software SSRLocator. The selected markers were validated using 56 plants sampled in seven populations from southern Brazil. The markers with potential polymorphism were selected and fluorescently labeled for characterization by capillary electrophoresis. In total, 92 SSR loci were found in G. chacoensis contigs. Suitable primers were designed for 70 SSR loci, and the remaining 22 SSR loci did not have sequences for primer development. Out of 35 selected SSR markers, after PCR optimization, 10 with high polymorphism potential were characterized. These loci can be used in genetic analyses of G. chacoensis and all of them were successfully transferred to other bamboo species. Non-polymorphic loci require further tests with additional plants, from different populations, to identify possibilities of their use.

5.
3 Biotech ; 9(11): 423, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31696028

RESUMO

Repetitive genomic elements were prospected in Campomanesia xanthocarpa, aiming to characterize these elements in a non-model plant species and to develop species-specific microsatellite markers. Approximately 4.12% of the partial genome of C. xanthocarpa is composed of repetitive elements, being retrotransposons the most widely represented. A total of nine polymorphic microsatellite markers were obtained: four nuclear-neutral, two nuclear EST, two plastidial and one mitochondrial. Levels of population genetic diversity of four natural populations of C. xanthocarpa were characterized using these markers. In addition, the cross-species amplification of the microsatellite markers was tested in seven species of tribe Myrteae (Myrtaceae). The characterized microsatellite markers revealed low to moderate levels of genetic diversity (expected heterozygosity range: 0.33-0.57; observed heterozygosity: 0.26-0.74 and number of alleles: 2.25-4.25). Cross-species amplification was successful for all loci, except Cxant76. These nine markers will contribute for studies on genetic diversity, gene flow, plant selection and breeding of this species, towards the conservation of natural populations, as well as its commercial use.

6.
Sci Rep ; 9(1): 4727, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30874613

RESUMO

A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper.

7.
Ecol Evol ; 7(22): 9461-9472, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-29187982

RESUMO

The flow of transgenes into landraces and wild relatives is an important biosafety concern. The case of transgene flow into local maize varieties in Mexico (the center of origin of maize) has been intensively debated over the past 15 years, including legal, political, and environmental disputes fanned by the existence of a significant scientific controversy over the methods used for the detection of transgenes. The use of diverse approaches and a lack of harmonized methods specific to the detection and monitoring of transgenes in landraces have generated both positive and negative results regarding contamination of Mexican maize with genetically modified material over the years. In this paper, we revisit the case of transgene contamination in Mexican maize and present a novel research approach based on socio-biological analysis of contrasting communities and seed management systems. Two communities were used to investigate how different social and biological factors can affect transgene flow and impact transgene spread in Mexico. Our results show the presence of transgenes in one community and thus support the position that transgenes are highly likely to be present in Mexican maize landraces. However, our work also demonstrates that the extent and frequency with which transgenes can be found will significantly depend on the societal characteristics and seed management systems of the local communities. Therefore, we argue that future analysis of transgene presence should include social research on the seed management practices in the sampling area so that more robust and comprehensive understandings and conclusions can be drawn.

8.
Sci Rep ; 6: 37855, 2016 12 19.
Artigo em Inglês | MEDLINE | ID: mdl-27991589

RESUMO

Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as 'substantially equivalent' to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent.


Assuntos
Resistência a Herbicidas/genética , Herbicidas/química , Plantas Geneticamente Modificadas/metabolismo , Proteoma , Zea mays/metabolismo , Cadaverina/química , Cromatografia Líquida , Ciclo do Ácido Cítrico , Biologia Computacional , Genes de Plantas , Glicina/análogos & derivados , Glicólise , Espectrometria de Massas , Metaboloma , Estresse Oxidativo , Plantas Geneticamente Modificadas/genética , Poliaminas/química , Putrescina/análogos & derivados , Putrescina/química , Espectrometria de Massas em Tandem , Zea mays/genética , Glifosato
9.
Appl Plant Sci ; 2(6)2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25202632

RESUMO

PREMISE OF THE STUDY: Microsatellite primers were identified and characterized in Acca sellowiana in order to expand the limited number of pre-existing polymorphic markers for use in population genetic studies for conservation, phylogeography, breeding, and domestication. • METHODS AND RESULTS: A total of 10 polymorphic microsatellite primers were designed from clones obtained from a simple sequence repeat (SSR)-enriched genomic library. The primers amplified di- and trinucleotide repeats with four to 27 alleles per locus. In all tested populations, the observed heterozygosity ranged from 0.269 to 1.0. • CONCLUSIONS: These new polymorphic SSR markers will allow future genetic studies to be denser, either for genetic structure characterization of natural populations or for studies involving genetic breeding and domestication process in A. sellowiana.

10.
Ciênc. cult. (Säo Paulo) ; 50(6): 408-16, Nov.-Dec. 1998.
Artigo em Inglês | LILACS | ID: lil-234531

RESUMO

The emergence of biotechnologies and new plant breeding programs occurs in a contex of the green revolution based system. These programs could be based on the rational use of biodiversity and directed to sustainable agriculture. In Third World countries the system shows signs of exhaustion. Brazil is the country with the world's largest genetic diversity of plants, comprising over 55,000 identified species of an estimated total ranging from 350,000 to 550,000. The use of such biodiversity brings about the discussion on the interests of northern hemisphere countris - rich in financial resources and technology yet poor in genetic resources - and Southern Hemisphere countris - poor in financial resources and rechnology but very rich in biological diversity. In these countries the so-called appropriate biotechnologies may become "windows of opportunities" for the characterization, conservation and use of such diversity. It is urgent, however, that these countries create conditions and capacities to characterize, conserve and use their resources in the short term.


Assuntos
Agricultura , Biotecnologia , Ecossistema , Brasil , Variação Genética
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