Evaluation of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (EBUS-TBNA) Samples from Advanced Non-Small Cell Lung Cancer for Whole Genome, Whole Exome and Comprehensive Panel Sequencing.

Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is often the only source of tumor tissue from patients with advanced, inoperable lung cancer. EBUS-TBNA aspirates are used for the diagnosis, staging, and genomic testing to inform therapy options. Here we extracted DNA and RNA from 220 EBUS-TBNA aspirates to evaluate their suitability for whole genome (WGS), whole exome (WES), and comprehensive panel sequencing. For a subset of 40 cases, the same nucleic acid extraction was sequenced using WGS, WES, and the TruSight Oncology 500 assay. Genomic features were compared between sequencing platforms and compared with those reported by clinical testing. A total of 204 aspirates (92.7%) had sufficient DNA (100 ng) for comprehensive panel sequencing, and 109 aspirates (49.5%) had sufficient material for WGS. Comprehensive sequencing platforms detected all seven clinically reported tier 1 actionable mutations, an additional three (7%) tier 1 mutations, six (15%) tier 2-3 mutations, and biomarkers of potential immunotherapy benefit (tumor mutation burden and microsatellite instability). As expected, WGS was more suited for the detection and discovery of emerging novel biomarkers of treatment response. WGS could be performed in half of all EBUS-TBNA aspirates, which points to the enormous potential of EBUS-TBNA as source material for large, well-curated discovery-based studies for novel and more effective predictors of treatment response. Comprehensive panel sequencing is possible in the vast majority of fresh EBUS-TBNA aspirates and enhances the detection of actionable mutations over current clinical testing.

Genetic parameters for body weight, carcass chemical composition and yield in a broiler-layer cross developed for QTL mapping

The objective of this study was to estimate genetic and phenotypic correlations of body weight at 6 weeks of age (BW6), as well as final carcass yield, and moisture, protein, fat and ash contents, using data from 3,422 F2 chickens originated from reciprocal cross between a broiler and a layer line. Variance components were estimated by the REML method, using animal models for evaluating random additive genetic and fixed contemporary group (sex, hatch and genetic group) effects. The heritability estimates (h²) for BW6, carcass yield and percentage of carcass moisture were 0.31 ± 0.07, 0.20 ± 0.05 and 0.33 ± 0.07, respectively. The h² for the percentages of protein, fat and ash on a dry matter basis were 0.48 ± 0.09, 0.55 ± 0.10 and 0.36 ± 0.08, respectively. BW6 had a positive genetic correlation with fat percentage in the carcass, but a negative one with protein and ash contents. Carcass yield, thus, appears to have only low genetic association with carcass composition traits. The genetic correlations observed between traits, measured on a dry matter basis, indicated that selection for carcass protein content may favor higher ash content and a lower percentage of carcass fat.

Avaliaçäo das características nutricionais de duas variedades de soja BRM 9550293 (alta proteína bruta) e BRS 133 (convencional) através de ensaios de desempenho em camundongos / Evaluation of the nourishing features of two different kinds of soybeans BRM 9550293 (high brute protein) and BRS 133 (conventional) through mice performance

O objetivo deste trabalho foi avaliar as caractarísticas nutricionais de duas variedades de soja em diversas diluíçöes, através de ensaios de desempenho em camundongos. Neste século para os próximos anos, estima-se um aumento considerável da populaçäo mundial e que uma parte desta irá sofrer de subnutriçäo. Para contribuir com a diminuíçäo deste processo decadente é necessário rever a importância do maior investimento na produçäo científica, tanto na criaçäo de novas técnicas de produçäo de alimentos, como novas fontes de nutrientes de baixo custo e com valor nutricional adequado à alimentaçäo humana. Com o objetivo de minimizar os custos e viabilizar o experimento em produçäo animal pode-se utilizar animais de laboratório para tais fins. No presente estudo foram utilizados 60 camundongos heterogêneos com idade de 21 dias de peso médio de 11 gramas e distribuídos aleatoriamente. Foram testados os seis tratamentos, três de cada variedade de soja, BRS 133 (convencional) e BRM 9550293 (alta proteína bruta), cada uma com diluíçöes diferentes. As diluiçöes referidas no presente estudo näo interferiram no desempenho dos camundongos.(au)